International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13647 - 13647
Published: Dec. 20, 2024
We
aim
to
investigate
whether
chemical
inhibition
of
NRF2
transcriptional
activity
(TA)
influences
distal
colon
contractions,
particularly
in
an
age-dependent
manner
females,
and
it
impacts
oestrogen
receptor
signalling
female
mice.
This
study
was
performed
on
3
6-month-old
mice
treated
with
ML385
(30
mg/kg)
or
a
vehicle
for
7
days
(i.p.).
The
functionality
verified
bead
expulsion
test;
serum
samples
were
collected
oestradiol
levels,
stored
various
histological
analyses.
results
show
that
the
seven-day
treatment
significantly
downregulated
TA
(p
<
0.05)
impacted
its
contractility.
Additionally,
young
females
exhibited
increase
goblet
cell
number
increased
ERα,
but
not
ERβ,
especially
older
It
is
worth
noting
basal
level
membrane
GPR30
higher
within
epithelial
layer,
led
downregulation
In
summary,
decreases
contractility
numbers.
expression
receptors
colons
Antioxidants,
Journal Year:
2025,
Volume and Issue:
14(1), P. 104 - 104
Published: Jan. 17, 2025
Breast
cancer
is
one
of
the
most
prevalent
cancers
worldwide.
Recent
studies
have
increasingly
emphasized
role
oxidative
stress
in
initiation
and
progression
breast
cancer.
This
article
reviews
how
imbalance
influences
occurrence
advancement
cancer,
elucidating
intricate
mechanisms
through
which
reactive
oxygen
species
(ROS)
operate
this
context
their
potential
therapeutic
applications.
By
highlighting
these
critical
insights,
review
aims
to
enhance
our
understanding
as
a
target
for
innovative
strategies
management
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(1), P. 149 - 149
Published: Jan. 19, 2025
Parkinson’s
disease
(PD)
is
a
prevalent
neurodegenerative
disorder
marked
by
the
progressive
degeneration
of
midbrain
dopaminergic
neurons
and
resultant
locomotor
dysfunction.
Despite
over
two
centuries
recognition
as
chronic
disease,
exact
pathogenesis
PD
remains
elusive.
The
onset
progression
involve
multiple
complex
pathological
processes,
with
dysfunctional
autophagy
elevated
oxidative
stress
serving
critical
contributors.
Notably,
emerging
research
has
underscored
interplay
between
in
pathogenesis.
Given
limited
efficacy
therapies
targeting
either
dysfunction
or
stress,
it
crucial
to
elucidate
intricate
mechanisms
governing
their
develop
more
effective
therapeutics.
This
review
overviews
role
nuclear
factor
erythroid
2-related
2
(Nrf2),
pivotal
transcriptional
regulator
orchestrating
cellular
defense
against
these
processes.
By
elucidating
key
processes
PD,
this
will
deepen
our
comprehensive
understanding
multifaceted
underlying
may
uncover
potential
strategies
for
its
prevention
treatment.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(3), P. 1110 - 1110
Published: Jan. 27, 2025
High-altitude
cerebral
edema
(HACE)
is
a
disorder
caused
by
low
pressure
and
hypoxia
at
high
altitudes.
Nevertheless,
as
of
now,
there
still
scarcity
safe
effective
prevention
treatment
methods.
The
active
component
Ligusticum
Chuanxiong,
namely
Ligustrazine
hydrochloride
(LH),
has
shown
potential
in
the
HACE
due
to
its
anti-inflammatory,
antioxidant,
neuroprotective
effects
nervous
system
disorders.
Consequently,
protective
effect
LH
on
mechanism
need
be
further
explored.
Prior
modeling,
90
male
Sprague-Dawley
rats
were
pretreated
with
different
doses
drugs,
including
(100
mg/kg
50
mg/kg),
dexamethasone
(4
ML385
(30
mg/kg).
Subsequently,
placed
low-pressure
anoxic
chamber
simulating
plateau
environment
establish
rat
model.
mechanisms
elucidated
through
determination
brain
water
content,
HE
staining,
ELISA,
immunofluorescence,
molecular
docking,
dynamics
simulation,
western
blot,
other
techniques.
results
showed,
first
all,
that
pretreatment
can
effectively
reduce
content;
down-regulate
expression
AQP4,
HIF-1α,
VEGF
proteins;
alleviate
damage
tissue
nerve
cells.
Secondly,
compared
group,
significantly
MDA
levels
increase
GSH
SOD
levels.
Additionally,
decreased
inflammatory
factors
IL-1β,
IL-6,
TNF-α;
reduced
total
iron
content
tissue;
increased
ferroptosis-related
proteins
such
SLC7A11,
GPX4,
FTH1;
alleviated
ferroptosis
occurrence.
Molecular
docking
simulations
show
strong
binding
affinity
for
NRF2
signaling.
Western
blot
analysis
confirmed
promotes
translocation
from
cytoplasm
nucleus
activates
signaling
pathway
exert
an
antioxidant
effect.
inhibitor
reverse
anti-oxidative
stress
tissue.
In
summary,
may
have
activating
pathway,
inhibiting
ferroptosis,
resisting
oxidative
stress.
Antioxidants,
Journal Year:
2025,
Volume and Issue:
14(1), P. 81 - 81
Published: Jan. 12, 2025
This
narrative
review
explores
the
potential
effects
of
Propolis
and
its
bioactive
compounds
on
bone
health.
Propolis,
a
resinous
product
collected
by
bees,
is
renowned
for
antimicrobial,
anti-inflammatory,
antioxidant
properties.
Recent
research
emphasizes
positive
role
in
osteogenesis,
primarily
through
modulation
osteoclast
osteoblast
activity
via
molecular
pathways.
Key
mechanisms
include
reducing
inflammatory
cytokines,
protecting
against
oxidative
stress,
upregulating
growth
factor
essential
formation.
While
such
as
Caffeic
Acid
Phenethyl
Ester,
Apigenin,
Quercetin,
Ferulic
have
been
well-documented,
emerging
evidence
points
to
significant
roles
less-studied
like
Pinocembrin,
Kaempferol,
p-Coumaric
acid,
Galangin.
synthesizes
current
literature,
focusing
which
these
influence
osteogenesis.
Firstly,
it
techniques
characterizing
presented
propolis,
chemogeographic
variations
composition,
both
crude
extracts
isolated
tissue,
offering
comprehensive
analysis
recent
findings
across
different
experimental
models.
Further,
discusses
In
summary,
modulate
signaling
pathways,
including
nuclear
kappa
beta,
wingless-related
integration
site,
mitogen-activated
protein
kinase,
vascular
endothelial
factor,
reactive
oxygen
species.
These
pathways
receptor
activator
kappa-β/receptor
kappa-β
ligand/osteoprotegerin
system,
fostering
cell
differentiation.
regulation
mitigates
excessive
formation,
stimulates
activity,
ultimately
contributes
restoration
homeostasis
maintaining
balanced
remodeling
process.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(3), P. 902 - 902
Published: Jan. 22, 2025
Aging
and
pregnancy
are
often
considered
opposites
in
a
woman’s
biological
timeline.
is
defined
by
gradual
decline
the
functional
capabilities
of
an
organism
over
its
lifetime,
while
characterized
presence
transient
placenta,
which
fosters
cellular
fitness
necessary
to
support
fetal
growth.
However,
context
preeclampsia,
aging
share
common
hallmarks,
including
clinical
complications,
altered
phenotypes,
heightened
oxidative
stress.
Furthermore,
women
with
pregnancies
complicated
preeclampsia
tend
experience
age-related
disorders
earlier
than
those
healthy
pregnancies.
Klotho,
gene
discovered
fortuitously
1997
researchers
studying
mechanisms,
primarily
expressed
kidneys
but
also
lesser
extent
several
other
tissues,
placenta.
The
Klotho
protein
membrane-bound
that,
upon
cleavage
ADAM10/17,
released
into
circulation
as
soluble
(sKlotho)
where
it
plays
role
modulating
This
review
focuses
on
involvement
sKlotho
development
disorders,
well
expression
recently
Mytho
gene,
has
been
associated
skeletal
muscle
atrophy.
Pharmaceuticals,
Journal Year:
2025,
Volume and Issue:
18(3), P. 345 - 345
Published: Feb. 27, 2025
Background:
Ophioglossum
vulgatum
Linn.
is
a
medical
herb
widely
distributed
in
Southwest
China.
It
has
been
used
for
the
treatment
of
various
diseases,
including
wounds
or
dermatitis,
since
ancient
times,
but
little
known
about
its
pharmacological
and
pharmaceutical
chemistry.
Methods:
The
ethyl
acetate
fraction
O.
(OpvE)
was
prepared
with
reflex
extraction
fractional
method.
Its
components
were
detected
identified
UPLC-Q/TOF-MS
system
SCIEX
OS
database.
related
biological
activities
underlying
mechanisms
predicted
by
computational
analysis.
HaCaT
cells
treated
gradient
concentrations
OpvE,
CCK-8
assay
performed
to
determine
cell
viability.
OpvE-pretreated
exposed
H2O2
LPS
antioxidative
anti-inflammatory
assessment.
DPPH,
GSH,
SOD,
MDA
kits
evaluate
oxidative
stress.
A
serially
diluted
microbiota
disc
diffusion
anti-Staphylococcus
aureus
activities.
transcription
genes
semi-quantitatively
studied
reversed
real-time
PCR.
Protein
levels
determined
western
blotting.
Results:
extract
ratio
OpvE
2.00
±
0.12%
(g/g).
total
21
ingredients
identified.
analysis
found
that
PI3K/Akt
signaling
pathway
might
be
crucial
target
OpvE.
(7.5~125
μg/mL)
stimulated
proliferation
migration
stimulating
over-expressed
collagen
type
I
alpha
1
Chain
(COL1A1)
fibronectin
(FN1)
upregulating
PI3K/AKT/GSK3-β
pathway.
In
test,
250
μg/mL
scavenged
obvious
97.28%
DPPH-released
free
radicals.
Pretreatment
inhibited
H2O2-induced
stress
protected
against
LPS-induced
inflammatory
injury
respectively
regulating
Nrf2/HO-1/COX2
TLR4/MYD88
pathways.
also
showed
anti-S.
properties
MIC
1.2
mg/mL,
this
concentration,
produced
an
8.3
0.16
mm
inhibition
zone
on
bacterial
plate.
Conclusions:
This
work
highlighted
phytochemical
character
some
bioactivities,
as
well
underline
mechanism,
which
would
support
further
studies
application
Nutrients,
Journal Year:
2025,
Volume and Issue:
17(5), P. 907 - 907
Published: March 5, 2025
Background:
Sweet
almond
expeller
is
an
abundant
protein
resource,
but
there
are
few
studies
on
multifunctional
peptides.
The
purpose
of
this
study
to
improve
its
application
in
food
and
medical
industries.
Methods:
This
investigated
the
identification,
screening,
action
mechanisms
antihypertensive
peptides
with
antioxidant
ferrous
binding
activities
derived
from
sweet
globulin
hydrolysates
using
intergrade
vitro
silico
methods
animal
model.
Results:
Eight
novel
oligopeptides
were
identified
subfraction
D;
them,
Pro-Met-Tyr-Gly-Gly-Gly-Met-Val
(PMYGGGMV)
exhibited
ACE
inhibitory
activity
(IC50:
121.16
μmol/L),
ability
(11.01
mg/g),
quenching
capacities
hydroxyl
(93.06%)
ABTS
radicals
(83.67%).
phenolic
hydroxyl,
amino,
carboxyl
groups
PMYGGGMV
linked
Lys511,
Tyr520,
Tyr523
ACE's
substrate
center
through
four
short
hydrogen
bonds.
can
inhibit
Kelch-like
ECH-Associated
Protein
1
(Keap1)-nuclear
factor
erythroid
2-related
2
(Nrf2)
interaction
by
seven
residues
Keap1
(including
a
key
residue,
Arg415).
PMYGGMY
stable
during
gastrointestinal
digestion.
Ferrous
chelation
did
not
alter
effects
PMYGGMY,
it
reduced
radical
scavenging
(p
<
0.05).
Additionally,
blood
pressure
spontaneous
hypertension
rates
improved
iron
absorption
across
Caco-2
cells
Conclusions:
has
potential
prevent
oxidative
stress,
hypertension,
deficiency.
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(7), P. 2927 - 2927
Published: March 24, 2025
The
purpose
of
the
present
work
was
to
establish
transportation
conditions
for
keeping
Trachinotus
ovatus
alive
by
means
corona-induced
dormancy
in
combination
with
taurine.
It
also
investigated
synergistic
regulatory
effects
on
oxidative
stress
mitigation
and
immune
function
during
low-temperature
clarified
underlying
mechanism.
pretreatment
induced
pulsed
direct
current
could
reduce
accumulation
reactive
oxygen
species
fish
under
hypothermal
water-restricted
significantly
enhance
environmental
adaptability
ovatus.
survival
period
rate
were
increased
when
combined
taurine
at
a
concentration
70
mg/L,
activities
enzymes
related
significantly,
including
catalase,
superoxide
dismutase
(SOD),
glutathione
S-transferase,
so
on.
mechanism
involved
upregulation
mRNA
expression
Nrf2/Keap1
pathway
components.
Furthermore,
supplementation
found
bolster
Histological
examinations
revealed
that
exerted
protective
ultrastructural
integrity
liver
gills,
which
susceptible
stress-induced
damage
transportation.
Altogether,
concerted
action
corona
mitigated
responses
tissue
injuries
live
transportation,
thereby
providing
mechanistic
basis
enhancement
techniques.