Approaches to Enhance Abiotic and Biotic Stress Tolerance in Leguminous Crops and Microgreens

Published: Jan. 1, 2025

Language: Английский

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Harnessing endophytes: innovative strategies for sustainable agricultural practices

Discover Bacteria., Journal Year: 2025, Volume and Issue: 2(1)

Published: Feb. 22, 2025

Language: Английский

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Non-Rhizobial Endophytes (NREs) of the Nodule Microbiome Have Synergistic Roles in Beneficial Tripartite Plant–Microbe Interactions

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 518 - 518

Published: Feb. 26, 2025

Microbial symbioses deal with the symbiotic interactions between a given microorganism and another host. The most widely known investigated microbial symbiosis is association leguminous plants nitrogen-fixing rhizobia. It one of best-studied plant–microbe that occur in soil rhizosphere oldest extensively studied for past several decades globally. Until recently, it used to be common understanding among scientists field rhizobia ecology root nodules thousands species only contain With advancement molecular microbiology coming into being state-of-the-art biotechnology innovations, including next-generation sequencing, has now been revealed living legumes are not alone. Microbiome studies such as metagenomics nodule community showed that, addition rhizobia, other bacteria referred non-rhizobial endophytes (NREs) exist nodules. This review provides an insight occurrence legume beneficial roles tripartite legumes, (NREs).

Language: Английский

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Enhancing drought tolerance in Pisum sativum and Vicia faba through interspecific interactions with a mixed inoculum of Rhizobium laguerreae and non-host beneficial rhizobacteria

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 26, 2025

Harnessing plant growth-promoting rhizobia presents a sustainable and cost-effective method to enhance crop performance, particularly under drought stress. This study evaluates the variability of (PGP) traits among three strains Rhizobium laguerreae LMR575, LMR571, LMR655, two native PGP Bacillus LMR698 Enterobacter aerogenes LMR696. The primary objective was assess host range specificity these their effectiveness in improving tolerance legume species: Pisum sativum, Vicia faba, Phaseolus vulgaris. In-vitro experiments were conducted selected strains, including phosphate solubilization, indole-3-acetic acid (IAA) production, siderophore production. Greenhouse trials also performed using mixed inoculum performing evaluate effects on physiological biochemical conditions. Significant observed strains. R. LMR655 exhibited highest solubilization (113.85 mg mL-1 PO4 2-), while LMR571 produced IAA concentration (25.37 mL-1). E. LMR696 demonstrated 82% Symbiotic interactions varied, with forming associations P. sativum V. but none establishing compatibility showed that significantly improved proline, total soluble sugars, proteins, chlorophyll content stress, faba showing strongest response. These findings highlight importance strain selection based potential. enhanced suggests tailored microbial inoculants can improve resilience water-limited environments. provides valuable insights for optimizing bioinoculant formulations performance

Language: Английский

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Effect of Ascochyta rabiei on symbiotic efficiency and productivity of Cicer arietinum

Regulatory Mechanisms in Biosystems, Journal Year: 2025, Volume and Issue: 33(1), P. e25001 - e25001

Published: Jan. 9, 2025

The pathological process reduces the intensity of metabolic processes in plants but also negatively affects symbiotic activity legumes. Ascochyta blight is most common disease chickpeas Ukraine. study aimed to determine influence Asc o chyta rabiei (Pass.) on dynamics number and weight nodules root system during growing season formation grain yield. Pathogenic microflora had a significant impact development chickpea apparatus. Diseased smaller mass nodules. A comparative analysis results our exper i ment showed that YeS Alunt variety formed more We found nu m ber both experimental varieties were lower: Odysei lowest, with strong degree ascochyta damage 3 points. In flowering phase (BBCH 60 – 70), was 86% 89%, respectively, for control variant. active diseased this indicator behind by 25% budding 51 59) 40% 70) variety; 38% 28% variety. On average, over two years research, yield 2.25 t/ha 2.21 accordance pattern apparatus, parallel dependence infection plants. Chickpea weak at harvest decrease 5.3% (YeS Alunt) 7.2% (Odysei). average led shortfall yield, 12.0% 11.3%. variety, variant intense damage, 19.1%, 18.1%.

Language: Английский

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Enhancing Pisum sativum growth and symbiosis under heat stress: the synergistic impact of co-inoculated bacterial consortia and ACC deaminase-lacking Rhizobium

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 4, 2024

Abstract The 1-aminocyclopropane-1-carboxylate (ACC) deaminase is a crucial bacterial trait, yet it not widely distributed among rhizobia. Hence, employing co-inoculation approach that combines selected plant growth-promoting bacteria with compatible rhizobial strains, especially those lacking ACC deaminase, presents practical solution to alleviate the negative effects of diverse abiotic stresses on legume nodulation. Our objective was explore efficacy three non-rhizobial endophytes, Phyllobacterium salinisoli (PH), Starkeya sp. (ST) and Pseudomonas turukhanskensis (PS), isolated from native legumes grown in Tunisian arid regions, improving growth cool-season fostering symbiosis an deaminase-lacking strain under heat stress. Various combinations these endophytes (ST + PS, ST PH, PS PH) were co-inoculated Rhizobium leguminosarum 128C53 or its Δ acdS mutant derivative Pisum sativum plants exposed two-week stress period. findings revealed absence activity negatively impacted both pea Nevertheless, detrimental successfully mitigated specific consortia. results indicated significantly altered phenolic content root exudates. Despite this, there no impact IAA production. Interestingly, changes positively influenced biofilm formation consortia containing strain, indicating synergistic bacteria-bacteria interactions. Additionally, positive observed when endophytic combined wild-type strain. This study highlights potential improve symbiotic performance strains genetic mechanisms mitigate their host, holding promising enhance yield targeted by boosting symbiosis.

Language: Английский

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Harnessing chickpea bacterial endophytes for improved plant health and fitness

AIMS Microbiology, Journal Year: 2024, Volume and Issue: 10(3), P. 489 - 506

Published: Jan. 1, 2024

<p>Endophytic bacteria live asymptomatically inside the tissues of host plants without inflicting any damage. Endophytes can confer several beneficial traits to plants, which contribute their growth, development, and overall health. They have been found stimulate plant growth by enhancing nutrient uptake availability. produce growth-promoting substances such as auxins, cytokinins, gibberellins, regulate various aspects development. also improve root system architecture, leading increased water absorption. Some endophytes possess ability solubilize nutrients, phosphorus potassium, making them more available for uptake, fixing atmospheric nitrogen. Chickpea (<italic>Cicer arietinum</italic>) is a major legume crop that has mutualistic interactions with endophytes. These benefit chickpea in ways, including higher improved tolerance abiotic biotic stressors, disease suppression. enzymes metabolites scavenge harmful reactive oxygen species, thus reducing oxidative stress. Moreover, studies reported antimicrobial compounds, lytic enzymes, volatile organic compounds inhibit fungal pathogens trigger systemic defense responses resistance against broad range pathogens. activate pathways, production defense-related phytoalexins, pathogenesis-related proteins, thereby providing long-lasting protection. It important note diversity function chickpea-associated vary depending on factors variety, geographical location, environmental conditions. The mechanisms behind plant-beneficial are still being intensively explored. In this review, new biotechnologies agricultural ecosystem stability were presented. Thus, harnessing could be exploited developing drought-resistant cultivars maintain productivity arid semi-arid environments, crucial meeting global demand chickpeas.</p>

Language: Английский

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Sustainable Strategy to Boost Legumes Growth under Salinity and Drought Stress in Semi-Arid and Arid Regions

Soil Systems, Journal Year: 2024, Volume and Issue: 8(3), P. 84 - 84

Published: July 23, 2024

The escalating risks of drought and salinization due to climate change anthropogenic activities are a major global concern. Rhizobium–legume (herb or tree) symbiosis is proposed as an ideal solution for improving soil fertility rehabilitating arid lands, representing crucial direction future research. Consequently, several studies have focused on enhancing legume tolerance salinity stresses using various techniques, including molecular-based approaches. These methods, however, costly, time-consuming, cause some environmental issues. multiplicity beneficial effects microorganisms, particularly plant growth-promoting bacteria (PGPB) plant-associated microbiomes, can play role in performance productivity under harsh conditions zones. PGPB act directly indirectly through advanced mechanisms increase water uptake, reduce ion toxicity, induce resilience osmotic oxidative stress. For example, rhizobia with legumes enhance growth not only by fixing nitrogen but also solubilizing phosphates producing phytohormones, among other mechanisms. This underscores the need further strengthen research its application modern agriculture. In this review, we provide comprehensive description challenges faced nitrogen-fixing leguminous plants semi-arid environments, salinity. We highlight potential benefits legume–rhizobium combined establish more sustainable agricultural practices these regions legume–rhizobium–PGPB partnerships.

Language: Английский

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Rhizobia inoculation’s impact on the biomass and moisture content of leguminous Bambara groundnut (Vigna subterranean L. Verdc)

Discover Sustainability, Journal Year: 2024, Volume and Issue: 5(1)

Published: Oct. 9, 2024

Bambara groundnut (BG) (Vigna subterranean L. verdc) is a highly nutritious underutilized leguminous crop of African origin with high economic importance. Its production rate (300,000 metric tonnes/year) does not meet the current demand (800,000 tonnes/year). Despite it's useful properties, it has remained neglected, poor market and low yields because research about improving BG been neglected. rhizobia have proved to be in promoting growth legumes, yet, there unavailable information role indigenous BG-symbiont inoculant (which are better affordable alternatives chemical fertilizers) for increasing yield this needs explored, hence study. This experiment studied six rhizobial strains biomass plant vigour two selected varieties (TVSU 1248, TVSU 631). These were applied as (at concentrations > 0.85 × 1010 cfu/ml) on field compared alongside commercial USDA110 Urea (rates 60 kg/ha 20 kg/ha) treated plants analysed shoot, root, shaft seed, moisture etc. Rhizobia significantly influenced shoot (≥ 19.26 g), (79.81 ± 1.81 g 63.15 3.21 root 0.63 pod 155.3 seed 103.96 shaff 52.29 g) content plants, differences varietal response. Indigenous BG-symbiotic able influence biomass, health BG. Rhizobia, enhancing should recommended higher productivity.

Language: Английский

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