Antimicrobial, antioxidant, and cytotoxic properties of biosynthesized copper oxide nanoparticles (CuO-NPs) using Athrixia phylicoides DC

Heliyon, Journal Year: 2023, Volume and Issue: 9(4), P. e15265 - e15265

Published: April 1, 2023

Nanoparticles produced from various metal elements including copper have been used in the treatment of infectious diseases response to antibiotic failure due microbial resistance. Copper is recommended for use production nanoparticles largely because its accessibility and affordability. This study aimed synthesise oxide (CuO-NPs) using leaf extracts Athrixia phylicoides assess their antibacterial, antioxidant cytotoxicity properties. The characterization obtained NPs was done through X-ray diffraction (XRD), Fourier transforms infrared spectroscopy (FTIR), Scanning electron microscopy (SEM) Energy-dispersive (EDS). Our results showed that had a highly crystalline, quasi-spherical shape with an average diameter 42 nm. Also, gram-positive bacteria Bacillus cereus Staphylococcus aureus were most susceptible CuO-NPs MIC values 0.62 mg/mL 0.16 mg/mL, respectively, as shown by broth microdilution method. In addition, demonstrated strong radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibitory activity IC50 value 10.68 ± 0.03 μg/mL. However, determined (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) (MTT) assay revealed not toxic human embryonic kidney cells (HEK 293 cells) at LC50 66.08 0.55 synthesised high potency less toxicity. Therefore, they could be feasible alternative source therapeutic agents treating bacterial oxidative stress-induced diseases.

Language: Английский

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Green and cost-effective biofabrication of copper oxide nanoparticles: Exploring antimicrobial and anticancer applications

Biotechnology Reports, Journal Year: 2024, Volume and Issue: 41, P. e00828 - e00828

Published: Jan. 12, 2024

Nanotechnology has made remarkable advancements in recent years, revolutionizing various scientific fields, industries, and research institutions through the utilization of metal oxide nanoparticles. Among these nanoparticles, copper nanoparticles (CuO NPs) have garnered significant attention due to their versatile properties wide-range applications, particularly, as effective antimicrobial anticancer agents. CuO NPs can be synthesized using different methods, including physical, chemical, biological approaches. However, conventional chemical physical approaches are expensive, resource-intensive, involve use hazardous chemicals, which pose risks human health environment. In contrast, synthesis provides a sustainable cost-effective alternative by eliminating pollutants allowing for production tailored sizes shapes. This comprehensive review focused on green resources, such plants, microorganisms, other derivatives. Current knowledge trends methods discussed, with specific emphasis biomedical particularly combating cancer microbial infections. highlights potential addressing diseases. By capitalizing advantages synthesis, environmental safety ability customize nanoparticle characteristics, emerged promising therapeutic agents wide range conditions. presents compelling findings, demonstrating achievements biologically novel Their unique mechanisms enable against cells harmful exhibit potent activity diverse mechanisms, induction apoptosis, inhibition angiogenesis, modulation signaling pathways. Additionally, manifests disrupting membranes, generating reactive oxygen species, interfering enzymes. offers valuable insights into substantial an innovative approach future interventions

Language: Английский

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Synthesis, characterization, and advanced sustainable applications of copper oxide nanoparticles: a review

Clean Technologies and Environmental Policy, Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

Language: Английский

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Copper nanoparticles from chemical, physical, and green synthesis to medicinal application: A review

Plant Nano Biology, Journal Year: 2024, Volume and Issue: 8, P. 100070 - 100070

Published: April 16, 2024

This article examines the viability of utilizing plant biomolecules in environmentally sustainable synthesis copper nanoparticles (Cu NPs). By harnessing their intrinsic capabilities reduction and capping, plants present an friendly economically viable substitute for conventional techniques. Through manipulation diverse parameters throughout procedure, scientists are capable attaining Cu NPs desired shapes sizes, thereby customizing them to suit particular applications. review centers on owing its cost-effectiveness comparison other frequently utilized metals such as gold silver. It explores mechanisms that govern NPs. We conduct exhaustive analysis wide-ranging medical healthcare applications, emphasizing capacity fundamentally transform numerous treatment approaches. Furthermore, offers valuable perspectives alternative techniques, facilitating a comparative evaluation enabling well-informed choices be made regarding integration knowledge methods, wide range practical implementations, economic factors, objective this is furnish asset scientists, researchers, practitioners who investigating propel progress.

Language: Английский

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Evaluation of Anticancer Potential of Biogenic Copper Oxide Nanoparticles (CuO NPs) against Breast Cancer

Journal of Nanomaterials, Journal Year: 2022, Volume and Issue: 2022(1)

Published: Jan. 1, 2022

The present study evaluated the anticancer potential of copper oxide nanoparticles (CuO NPs) synthesized from pumpkin seed extract in human breast cancer cell line (MDA‐MB‐231) using a battery tests such as MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay, morphological alteration, reactive oxygen species (ROS) generation, and changes mitochondrial membrane (MMP). biogenic CuO NPs showed dose‐dependent decline viability with 50% inhibitory concentration (IC50) at 20 μ g/ml. Treatment an IC50 dose resulted considerable morphology changes, shrinkage, detachment, blebbing, deformed shape MDA‐MB‐231 cells. We also observed significant increase ROS production MMP modulation due to NP treatment. Overall, line. However, further validation our data is required ex vivo models before this nanoformulation could be exploited for treatment/management cancer.

Language: Английский

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Copper nanoparticles and their oxides: optical, anticancer and antibacterial properties

International nano letters., Journal Year: 2022, Volume and Issue: 12(4), P. 379 - 398

Published: Sept. 17, 2022

Language: Английский

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Synthesis of Nickel Oxide Nanoparticles and Copper-Doped Nickel Oxide Nanocomposites Using Phytolacca dodecandra L’Herit Leaf Extract and Evaluation of Its Antioxidant and Photocatalytic Activities

ACS Omega, Journal Year: 2022, Volume and Issue: 7(49), P. 44720 - 44732

Published: Dec. 1, 2022

Nanotechnology research is emerging as cutting-edge technology, and nanoparticles (NPs) nanocomposites (NCs) have played a significant role in the bioremediation treatment of polluted water by organic nonorganic materials. Nanoparticles produced plant extracts are more stable biocompatible comparison with those physical chemical methods. This focuses on synthesis NiO NPs Cu-NiO NCs using Phytolacca dodecandra L'Herit (P.d) leaf extract evaluation their antioxidant photocatalytic activities. were synthesized 50 mL 0.1 M nickel(II) nitrate hexahydrate, 10 copper(II) trihydrate, 20 extract. The characterized UV-vis, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) analyses to study energy band gap, average crystallite size, functional groups, morphology samples, respectively. UV-vis analysis showed red shift after copper doping, indicating decrease optical gap values. FT-IR characterization confirms presence various groups samples. Crystallite sizes formed particles obtained be 14.18 16.10 nm from XRD data for NCs, SEM crystallinity cubic structure. degradation methylene blue (MB) was found 78.3 97.8% In test, prevented oxidation 50% H2O2 molecules at concentration 363.96 350.29 μg/mL, Finally, samples good

Language: Английский

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Endophytic bacterial strain,Brevibacillus brevis-mediated green synthesis of copper oxide nanoparticles, characterization, antifungal,in vitrocytotoxicity, and larvicidal activity

Green Processing and Synthesis, Journal Year: 2022, Volume and Issue: 11(1), P. 931 - 950

Published: Jan. 1, 2022

Abstract The biomass filtrate containing various metabolites of endophytic bacterial strain, Brevibacillus brevis PI-5 was used as a biocatalyst for reducing and stabilizing copper oxide nanoparticles (CuO-NPs). UV-Vis spectroscopy, Fourier transform infrared, transmission electron microscopy, scanning microscopy with energy-dispersive X-ray (SEM-EDX), diffraction, photoelectron spectroscopy were CuO-NPs characterization. A spherical, well-dispersed, crystallographic structure sizes 2–28 nm formed. SEM-EDX confirmed the presence Cu O weight percentages 27.62% 48.88%, respectively. biological activities including antifungal, anticancer, larvicidal synthesized assessed using fungal radial growth inhibition, MTT assay method, mortality percentages, obtained data showed that exhibit high activity in dose-dependent manner. three phytopathogenic fungi, Fusarium oxysporum, Alternaria alternata , Aspergillus niger decreased by 64.5% ± 4.1%, 62.9% 0.3%, 70.2% 2.3%, respectively at 300 µg·mL –1 . Also, clinical Candida spp. successfully inhibited varied zones inhibition minimum inhibitory concentration values ranges 6.25–50 vitro cytotoxicity exhibits target-orientation to breast cancer cells (T47D) low compared normal (HFB4) IC 50 122.3 5.4 229.9 5.7 μg·mL I, II, III, IV instar larvae Culex antennatus 60% 1.4%, 43.1% 1.1%, 36.2% 1%, 32.1% 0.9%, 10 mg·L increased 86.9% 2.1%, 68.1% 1.7%, 64.4% 1.9%, 53.1% 1.4%

Language: Английский

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Green synthesis and characterization of copper nanoparticles using Phragmanthera austroarabica extract and their biological/environmental applications

Sustainable materials and technologies, Journal Year: 2022, Volume and Issue: 35, P. e00540 - e00540

Published: Dec. 5, 2022

Language: Английский

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Recyclability and catalytic characteristics of copper oxide nanoparticles derived from bougainvillea plant flower extract for biomedical application

Green Processing and Synthesis, Journal Year: 2023, Volume and Issue: 12(1)

Published: Jan. 1, 2023

Abstract This work aims to investigate the environmentally sustainable technique synthesize copper nanoparticles using bougainvillea flower ethanolic extract at ambient temperature. Copper have considerable potential for reducing environment’s harmful pigments and nitrogen contaminants. The oxidized nanoscale catalysts are enclosed inside nanomaterial, which as a benign resource capping agents. Ultraviolet spectroscopic, transmission electron microscopy (TEM), X-ray crystallography (XRD) techniques were used evaluate produced nanocrystals. particles been very robust, cylindrical in form, an outer diameter of 12 nm. Furthermore, under normal conditions, oxide (CuO) nanomaterials demonstrated strong photocatalytic efficiency liquid media oxidation Congo red, bromothymol blue, 4-nitrophenol acidic solution acetic anhydride. Moreover, CuO nanocrystalline enzyme could be readily vortexed or five cycles with exchange rate even over 90%. evaporation process caused around 18% loss weight between 25°C 190°C, while soil organic breakdown almost 31% 700°C. As result, little reduction enzymatic effectiveness recoverable multilayer substrate might attributed catalytic degradation throughout spinning processing.

Language: Английский

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