Thymoquinone, piperine, and sorafenib combinations attenuate liver and breast cancers progression: epigenetic and molecular docking approaches

BMC Complementary Medicine and Therapies, Journal Year: 2023, Volume and Issue: 23(1)

Published: March 4, 2023

Abstract Background Traditional herbal medicine has been used for centuries to cure many pathological disorders, including cancer. Thymoquinone (TQ) and piperine (PIP) are major bioactive constituents of the black seed ( Nigella sativa ) pepper Piper nigrum) , respectively. The current study aimed explore potential chemo-modulatory effects, mechanisms action, molecular targets, binding interactions after TQ PIP treatments their combination with sorafenib (SOR) against human triple-negative breast cancer (MDA-MB-231) liver (HepG2) cells. Methods We determined drug cytotoxicity by MTT assay, cell cycle, death mechanism flow cytometry. Besides, effect TQ, PIP, SOR treatment on genome methylation acetylation determination DNA methyltransferase DNMT3B ), histone deacetylase HDAC3 miRNA-29c expression levels. Finally, a docking was performed propose action affinity HDAC3. Results Collectively, our data show that combinations and/or have significantly enhanced anti-proliferative cytotoxic effects depending dose line enhancing G2/M phase arrest, inducing apoptosis, downregulation upregulation tumor suppressor, miRNA-29c. identified strong between SOR, HDAC3, inhibiting normal oncogenic activities leading growth arrest death. Conclusion This reported as enhancers antiproliferative addressed mechanisms, targets involved in action.

Language: Английский

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Pomegranate (Punica granatum L.) phytochemicals target the components of metabolic syndrome

The Journal of Nutritional Biochemistry, Journal Year: 2024, Volume and Issue: 131, P. 109670 - 109670

Published: May 19, 2024

Language: Английский

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Pomegranate Seeds and Peel Ethanolic Extracts Anticancer Potentials and Related Genetic, Histological, Immunohistochemical, Apoptotic and Oxidative Stress Profiles: In vitro Study

Journal of Experimental Pharmacology, Journal Year: 2023, Volume and Issue: Volume 15, P. 191 - 205

Published: April 1, 2023

Owing to their great quantity of hydrolyzable anthocyanins and tannins, the peel seeds pomegranate are edible possess potent anti-oxidant anti-inflammatory characteristics. This work aims trace seed ethanolic extracts' anticancer activity against liver cancer cell line, namely HepG2 related histopathological, immunohistochemical, genetic oxidative stress profile.In vitro study for both extract showed prevalence phenols, polyphenols acids, those have anti-proliferative potential line (HepG2) with 50% inhibitory concentration (IC50) significantly reduced that peel. Toxicity test extracts was dependent accompanied cycle arrest death at theG0/G1 S phases but not G2/M phase. Cell supplemented raised ROS, MDA decreased SOD, GSH Catalase.Apoptosis-related genes significant up-expression pro-apoptotic gene (P53), Cy-C, Bax, casp-3 down expression anti-apoptotic (Bcl-2). Also, Casp-3 P53 proteins were substantially expressed under effect extracts. Histopathological demonstrated untreated cells (control group) regular nuclear pleomorphism hyperchromatic nuclei, while extracts-treated necrosis, mixed euchromatin heterochromatin, intra-nuclear eosinophilic structures, burst membranes, shrunken apoptotic membranes irregular cells. Finally, PCNA detected by immunohistochemistry regulated treatment than in case medication extract.

Language: Английский

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Phytochemical properties and bioactive compounds of pomegranate ( Punica granatum L.)

The Journal of Horticultural Science and Biotechnology, Journal Year: 2024, Volume and Issue: 99(6), P. 639 - 652

Published: June 25, 2024

Language: Английский

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The preventive and therapeutic effects of anthocyanins on colorectal cancer: A comprehensive review based on up-to-date experimental studies

Food Research International, Journal Year: 2023, Volume and Issue: 170, P. 113028 - 113028

Published: May 24, 2023

Language: Английский

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Exploring Punicalagin Potential Against Cancers: A Comprehensive Review

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(3)

Published: Feb. 27, 2025

Punicalagin, being a bioactive polyphenol, has gained significant interest owing to its potential anticancer effects. Researchers are studying punicalagin determine ability inhibit cancer cell proliferation. This paper focuses on highlighting the therapeutic of against tumors and cancers. Punicalagin inhibits tumor growth within body through various cellular pathway interactions. compound effectively eliminates cells from liver, stomach, prostate, lungs. Different animal model studies have demonstrated in blocking MAPK/ERK PI3K/AKT/mTOR signaling pathways, fighting cancer. Through inhibition division, may be capable eradicating breast cells. strongly activities vascular endothelial factor (VEGF), matrix metalloproteinase-9 (MMP-9), uPA. androgen receptor (AR), protein essential for development metastasis prostate Lung can eliminated by initiating caspase cascade inhibiting synthesis, which is facilitated punicalagin. In numerous models, significantly reduces metastasis. Cyclokinase B1 cyclin-dependent kinase 2 promote colon cancer; when reach G2/M phase, induces apoptosis action these proteins. propagation blood vessel There still need further clinical trials fully reveal punicalagin's as well safety; despite fact that it decrease threat increasing provide an alternative treatment many

Language: Английский

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The Occurrence, Uses, Biosynthetic Pathway, and Biotechnological Production of Plumbagin, a Potent Antitumor Naphthoquinone

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1618 - 1618

Published: April 4, 2025

Plumbagin is an important naphthoquinone with potent anticancer properties besides multitudinous uses in healthcare. It produced a limited number of species and families but mostly the roots Plumbaginaceae family members. The biosynthetic pathway genes that regulate plumbagin synthesis are not completely known, details these being revealed. Several species, including Plumbago, Drosera, others, uprooted for extraction by pharmaceutical industries, leading to destruction natural habitats. industry therefore facing acute shortage plant material. This necessitates enhancing accumulation using suspensions hairy meet market demands. Many factors, such as aggregate size inoculum, stability culture, sequential effects elicitors, immobilization, permeabilization, have been demonstrated act synergistically markedly augment accumulation. Hairy root cultures can be used large-scale production, growth, accumulation, exploration their efficacy now imperative. secretion compounds into spent medium situ adsorption via resin has remarkable potential, this thoroughly exploited. Improvements quality biomass, selection cell lines, production bioreactors thus far sporadic, parameters need further In review, we report advances made relating importance stable line long-term cultures, plumbagin, its semicontinuous total recycling different types bioreactors. Such might pave way industrial exploitation. steps currently understood also aid us isolating relevant order examine overexpression or heterologous downregulation edit genome CRISPR-Cas9 technology enhance plumbagin. Its potential molecule mode action amply demonstrated, exploited clinics due insolubility water highly lipophilic nature. Plumbagin-loaded nanoemulsions, plumbagin–silver, albumin nanoparticle formulations overcome problems solubility tried improve bioavailability antiproliferative activities, discussed current paper.

Language: Английский

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Assessment of the Nutraceutical Properties of Wild Strawberry (Fragaria vesca L.) Extracts on Human Colorectal Cell Lines

Molecular Nutrition & Food Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

ABSTRACT Colorectal cancer is the third most common worldwide; its higher incidence in Western world, compared to rural areas of Africa and Asia, led classification among so‐called Westernized diseases. The wild strawberry Fragaria vesca L. endowed with several bioactive components, such as polyphenols, vitamins, terpenes, organic acids that can contribute prevention. In this study, we chemically characterized a extract through high‐resolution mass spectrometry evaluated antioxidant properties on two human colorectal cell lines: KRAS mutated SW480 cells E705 cells. We found treatment induced cycle arrest G2 phase cells, while it apoptosis significant increase reactive oxygen species level. Wild promising dietary supplement for both wild‐type KRAS‐mutated patients who exhibit more aggressive phenotype. addition, lack toxicity toward healthy makes food chemopreventive nutritional supplement.

Language: Английский

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Anticancer Potential of Tocopherols-Containing Plants and Semi-Synthetic Tocopherols

Plants, Journal Year: 2024, Volume and Issue: 13(21), P. 2994 - 2994

Published: Oct. 26, 2024

Tocopherols, potent bioactive compounds with anticancer properties, remain understudied in herbal medicinal plants, presenting a significant knowledge gap the field of natural agents. This review evaluates tocopherol-containing plants for their potential, analyzing Scopus publications from 2016 to 2024. Fifteen were identified as promising candidates, including Bulbine anguistifolia Poelln, Punica granatum L., Moringa oleifera, Kigelia pinnata, and Typhonium flagelliforme Lodd. The explores tocopherols’ mechanisms, apoptosis induction cell cycle arrest. Factors influencing effects are examined, such forms (α, β, γ, δ), concentrations, plant parts utilized, stability various plants. Additionally, emerging research on semi-synthetic tocopherol derivatives is analyzed, highlighting potential adjuvants chemotherapy role enhancing drug delivery reducing side effects. comprehensive analysis aims advance development plant-based pharmaceuticals improve cancer treatment strategies. By elucidating mechanisms this provides foundation future therapies. It emphasizes need further investigation into these plants’ potentially leading novel, more effective, less toxic findings presented here contribute nuanced understanding how can be leveraged drugs.

Language: Английский

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Antioxidants in Traditional Mexican Medicine and Their Applications as Antitumor Treatments

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(4), P. 482 - 482

Published: March 23, 2023

Traditional medicine in Latin America and mainly Mexico represents an essential alternative for treating different diseases. The use of plants as is the product a rich cultural tradition indigenous peoples, which great variety species are used treatment gastrointestinal, respiratory, mental diseases some other sicknesses; therapeutic efficacy that they possess due to properties derive from active ingredients principally antioxidants, such phenolic compounds, flavonoids, terpenes, tannins. An antioxidant substance that, at low concentrations, delays or prevents substrate oxidation through exchange electrons. Different methods determine activity most commonly described review. Cancer disease cells multiply uncontrollably spread parts body, process known metastasis. These can lead formation tumors, lumps tissue be cancerous (malignant) noncancerous (benign). Generally, this consists surgery, radiotherapy, chemotherapy, have side effects decrease quality life patients, so new treatments, focusing on natural resources plants, developed. This review aims gather scientific evidence compounds present traditional Mexican medicine, specifically antitumor common cancer types worldwide (e.g., breast, liver, colorectal cancer).

Language: Английский

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