Comparison of Evolutionary Relationships between Branchiostoma floridae, Ciona intestinalis, and Homo sapiens Globins Provide Evidence of Gene Co-Option and Convergent Evolution DOI Open Access

Nanako Yano,

Toshifumi Minamoto,

Hirosi Yamaguchi

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(21), P. 16009 - 16009

Published: Nov. 6, 2023

Globins have been studied as model proteins to elucidate the principles of protein evolution. This was achieved by understanding relationship between amino acid sequence, three-dimensional structure, physicochemical properties, and physiological function. Previous molecular phylogenies chordate globin genes revealed monophyletic evolution urochordate globins suggested convergent However, provide evidence evolution, it is necessary determine functional similarities vertebrates globins. In this study, we determined expression patterns Ciona using real-time RT-PCR. Two (Gb-1 Gb-2) were predominantly expressed in branchial sac, heart, hemocytes induced under hypoxia. Combined with sequence analysis, our findings suggest that Gb-1/-2 correspond vertebrate hemoglobin-α/-β. did not find a robust similarity Gb-3, Gb-4, These results that, even though obtained their unique functions differently from globins, two them shared some features functions. Our offer good example for mechanisms underlying gene co-option convergence, which could lead evolutionary innovations.

Language: Английский

Aortic Valve Stenosis Causes Accumulation of Extracellular Hemoglobin and Systemic Endothelial Dysfunction DOI Open Access
Christine Quast, Florian Bönner, Amin Polzin

et al.

Circulation, Journal Year: 2024, Volume and Issue: 150(12), P. 952 - 965

Published: June 5, 2024

BACKGROUND: Whether aortic valve stenosis (AS) can adversely affect systemic endothelial function independently of standard modifiable cardiovascular risk factors is unknown. METHODS: We therefore investigated and cardiac in an experimental model AS mice devoid human cohorts with scheduled for transcatheter replacement. Endothelial was determined by flow-mediated dilation using ultrasound. Extracellular hemoglobin (eHb) concentrations nitric oxide (NO) consumption were blood plasma humans ELISA chemiluminescence. This complemented measurements flow 4-dimensional acquisition magnetic resonance imaging computational fluid dynamics simulations. The effects red cell (RBC) suspensions on vascular transfer experiments a murine vasorelaxation bioassay system. RESULTS: In mice, the induction caused dysfunction. presence normal systolic left ventricular mild hypertrophy, increase transvalvular gradient associated elevated eryptosis, increased eHb, NO consumption; eHb sequestration haptoglobin restored function. Because orifice area patients decreased, postvalvular mechanical stress central ascending aorta increased. circulating RBC-derived microvesicles, eryptotic cells, lower levels without clinically relevant anemia, consecutive Transfer demonstrated that reduction treatment or elimination dynamic replacement subclinical RBC fragmentation, remaining RBCs before after exhibited intact membrane function, deformability, resistance to osmotic hypoxic stress. CONCLUSIONS: increases swirling aorta, triggering fragmentation accumulation plasma. blood, thereby limiting bioavailability. Thus, itself promotes dysfunction independent other established factors. Transcatheter capable scavenging rescuing realigning near physiological patterns. REGISTRATION: URL: https://www.clinicaltrials.gov ; Unique identifiers: NCT05603520 NCT01805739.

Language: Английский

Citations

5
Major heme proteins hemoglobin and myoglobin with respect to their roles in oxidative stress – a brief review DOI Creative Commons

Rajarshi Sil,

Abhay Sankar Chakraborti

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 25, 2025

Oxidative stress is considered as the root-cause of different pathological conditions. Transition metals, because their redox-active states, are capable free radical generation contributing oxidative stress. Hemoglobin and myoglobin two major heme proteins, involved in oxygen transport storage, respectively. Heme prosthetic group proteins a good reservoir iron, most abundant transition metal human body. Although iron tightly bound pocket these it liberated under specific circumstances yielding ferrous iron. This active can react with H2O2, secondary metabolite, forming hydroxyl via Fenton reaction. Hydroxyl harmful among all reactive species. It causes by damaging lipid membranes, nucleic acids, activating inflammatory pathways altering membrane channels, resulting disease In this review, we have discussed how heme-irons hemoglobin promote pathophysiological conditions including metabolic syndrome, diabetes, cardiovascular, neurodegenerative renal diseases. Understanding association to may be important for knowing complications well therapeutic management

Language: Английский

Citations

0
Development of a prognostic prediction model for non-smoking lung adenocarcinoma based on pathological information and laboratory hematologic indicators: a multicenter study DOI Creative Commons
Run Xiang,

Peihong Hu,

Xiaoxiong Xiao

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 14, 2025

To develop a simple and practical model to predict the prognostic survival of non-smoking patients with lung adenocarcinoma by combining general pathological information laboratory hematologic indicators. Cox univariate multivariate analyses were used identify variable A proportional hazards was constructed based on selected variables compare outcomes between high-and low-risk groups validate model's performance. Subsequently, nomogram established systematically evaluate impact prognosis. Data from four hospitals retrospectively collected. We enrolled 1,172 patients, this includes 372 external validation data. Multivariate analysis identified six significant (P < 0.05): tumor TNM stage, size, white blood cell count, neutrophil percentage, lymphocyte hemoglobin level. combined these build model. The C-index training set is 0.811 (0.780-0.842), value 0.786 (0.737-0.835) in,test 0.810 (0.772-0.847) in set. area under curve (AUC) results predicted 3-years overall (OS) three data sets 0.850, 0.819, 0.860, respectively. These values for 5-years 0.811, 0.771, 0.849. Stratified staging showed that effectively distinguished 0.0001). High-risk demonstrated significantly poorer prognosis compared 0.001). levels adenocarcinoma. Compared more studied markers at present, indicators our do not need conversion, Our provides useful reference personalized diagnosis treatment clinical practice.

Language: Английский

Citations

0
Hemoglobin in the brain frontal lobe tissue of patients with Alzheimer’s disease is susceptible to reactive nitrogen species-mediated oxidative damage DOI Creative Commons
Miranda J. Smallwood,

M. Abu Alghayth,

Annie R. Knight

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: unknown, P. 103612 - 103612

Published: March 1, 2025

Language: Английский

Citations

0
Allogenic Cord Blood Transfusion in Preterm Infants DOI
Luciana Teofili,

Patrizia Papacci,

Carmen Giannantonio

et al.

Clinics in Perinatology, Journal Year: 2023, Volume and Issue: 50(4), P. 881 - 893

Published: Aug. 17, 2023

Language: Английский

Citations

7
Changes in Hemoglobin Properties in Complex with Glutathione and after Glutathionylation DOI Open Access
Iuliia D. Kuleshova, П. И. Зарипов, Yuri M. Poluektov

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(17), P. 13557 - 13557

Published: Aug. 31, 2023

Hemoglobin is the main protein of red blood cells that provides oxygen transport to all human body. The ability hemoglobin bind low-molecular-weight thiol cell glutathione, both covalently and noncovalently, not only an important part antioxidant protection cells, but also affects its affinity for in cases. In this study, properties oxyhemoglobin complex with reduced glutathione (GSH) glutathionylated bound via SS bond were characterized. For purpose, methods circular dichroism, Raman spectroscopy, infrared tryptophan fluorescence, differential scanning fluorimetry, molecular modeling used. It was found glutathionylation caused changes secondary structure protein, reducing alpha helicity, did affect heme environment, thermostability protein. noncovalent remained almost unchanged; however, environment microenvironment tryptophans, as well a decrease protein's thermal stability, observed. Thus, formation makes more significant effect on tertiary quaternary than glutathionylation, which mainly obtained data are understanding functioning hemoglobin, marker oxidative stress, GSH, appears deposit GSH release it during deoxygenation increase cells.

Language: Английский

Citations

7
Erythrocytes Functionality in SARS-CoV-2 Infection: Potential Link with Alzheimer’s Disease DOI Open Access
Еlena Kosenko, Lyudmila Tikhonova, Gubidat Alilova

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5739 - 5739

Published: March 17, 2023

Coronavirus disease 2019 (COVID-19) is a rapidly spreading acute respiratory infection caused by SARS-CoV-2. The pathogenesis of the remains unclear. Recently, several hypotheses have emerged to explain mechanism interaction between SARS-CoV-2 and erythrocytes, its negative effect on oxygen-transport function that depends erythrocyte metabolism, which responsible for hemoglobin-oxygen affinity (Hb-O

Language: Английский

Citations

6
The artificial oxygen carrier erythrocruorin—characteristics and potential significance in medicine DOI Creative Commons
Weronika Kruczkowska, Mateusz Kciuk, Zbigniew Pasieka

et al.

Journal of Molecular Medicine, Journal Year: 2023, Volume and Issue: 101(8), P. 961 - 972

Published: July 18, 2023

Abstract The diminishing supply and increasing costs of donated blood have motivated research into novel hemoglobin-based oxygen carriers (HBOCs) that can serve as red cell (RBC) substitutes. HBOCs are versatile agents be used in the treatment hemorrhagic shock. However, many RBC substitutes based on mammalian hemoglobins presented key limitations such instability toxicity. In contrast, erythrocruorins (Ecs) other types may not suffer these disadvantages. Ecs giant metalloproteins found annelids, crustaceans, some invertebrates. Thus far, Lumbricus terrestris (LtEc) Arenicola marina (AmEc) most thoroughly studied. Based data from preclinical transfusion studies, it was compounds only efficiently transport anti-inflammatory properties, but also modified to further increase their effectiveness. This literature review focuses structure, application Ecs, well advantages over HBOCs. Development methods for both stabilization purification erythrocruorin could confer enhanced access artificial resources.

Language: Английский

Citations

6
Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment DOI Creative Commons

Lisa J. Neilson,

Douglas Cartwright, Maija Risteli

et al.

Matrix Biology Plus, Journal Year: 2023, Volume and Issue: 19-20, P. 100136 - 100136

Published: Nov. 22, 2023

High-grade serous (HGS) ovarian cancer is the most lethal gynaecological disease in world and metastases a major cause. The omentum preferential metastatic site HGS patients

Language: Английский

Citations

4
Bone Marrow Adipose Tissue: Impacts on Bone Marrow Stem Cell Niche and Hematopoietic System DOI
Drenka Trivanović, Ivana Drvenica

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 126 - 156

Published: Jan. 1, 2024

Language: Английский

Citations

1