Sculpting Secondary Structure of a Cyclic Peptide: Conformational Analysis of a Cyclic Hexapeptide Containing a Combination of l-Leu, d-Leu, and Aib Residues DOI Creative Commons
Takahito Ito, Hidetomo Yokoo, Takuma Kato

et al.

ACS Omega, Journal Year: 2023, Volume and Issue: 8(46), P. 44106 - 44111

Published: Nov. 8, 2023

We have previously reported that cyclo(l-Leu-d-Leu-Aib-l-Leu-d-Leu-Aib) (2), a cyclic hexapeptide consisting of heterochiral l-Leu and d-Leu (l-Leu-d-Leu) residues with achiral 2-aminoisobutyric acid (Aib) residues, forms figure-8 conformation. In this study, we newly designed cyclo(l-Leu-d-Leu-Aib-d-Leu-l-Leu-Aib)+ (4), an epimer 2, examined the conformational differences between 2 4 by X-ray crystallographic analysis. Peptide formed planar conformation antiparallel β-sheet hydrogen-bonding pattern. This investigation demonstrates potential to manipulate molecular peptides simply arranging l- d-amino acids emphasizes diverse conformations can be obtained using peptides. Harnessing as platforms for distinct structures is promising approach expanding chemical space various applications.

Language: Английский

Seaweed Proteins: A Step towards Sustainability? DOI Open Access
Leonel Pereira, João Cotas, Ana M. M. Gonçalves

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(8), P. 1123 - 1123

Published: April 10, 2024

This review delves into the burgeoning field of seaweed proteins as promising alternative sources protein. With global demand escalating and concerns over traditional protein sources' sustainability ethics, emerges a viable solution, offering high content minimal environmental impacts. Exploring nutritional composition, extraction methods, functional properties, potential health benefits proteins, this provides comprehensive understanding. Seaweed contains essential amino acids, vitamins, minerals, antioxidants. Its ranges from 11% to 32% dry weight, making it valuable for diverse dietary preferences, including vegetarian vegan diets. Furthermore, underscores advantages production compared sources. cultivation requires resources, mitigating issues like ocean acidification. As specific types, methodologies, highlights versatility in various food products, plant-based meats, dairy alternatives, supplements. Additionally, discusses associated with such their unique acid profile bioactive compounds. Overall, aims provide insights proteins' applications role addressing needs sustainably.

Language: Английский

Citations

16
Engineered Proteins and Materials Utilizing Residue-Specific Noncanonical Amino Acid Incorporation DOI Creative Commons

Temiloluwa Majekodunmi,

Dustin Britton, Jin Kim Montclare

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(15), P. 9113 - 9135

Published: July 15, 2024

The incorporation of noncanonical amino acids into proteins and protein-based materials has significantly expanded the repertoire available protein structures chemistries. Through residue-specific incorporation, properties can be globally modified, resulting in creation novel with diverse tailored characteristics. In this review, we highlight recent advancements techniques as well applications engineered materials. Specifically, discuss their utility bio-orthogonal acid tagging (BONCAT), fluorescent (FUNCAT), threonine-derived (THRONCAT), cross-linking, fluorination, enzyme engineering. This review underscores importance a tool for development to meet research industrial needs.

Language: Английский

Citations

7
Expanding the Scope of Azole-Based γ-Amino Acids DOI
Ludovic T. Maillard,

Samantha Chaise,

Audrey Gacogne

et al.

Synthesis, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Abstract The development of non-canonical amino acids is pivotal to peptide engineering, enabling the design molecules with novel structural features, improved activities, and optimized metabolic profiles. Among these, heteroaromatic γ-amino have attracted significant attention for their ability mimic native folds while accessing conformational spaces. In this study, chemical diversity was expanded by introducing two new monomers, ATC* AOC*, designed around a thiazole an oxazole scaffold, respectively. These analogues, characterized tunable substitution patterns precise stereochemical control significantly expand well-established ATC family.

Language: Английский

Citations

0
Identification of a Synthetic Peptide with Potential Angiotensin I-Converting Enzyme (ACE-1) Inhibitory Activity DOI
Camila Innocente-Alves,

Raul Izidoro Carneiro,

Bruna Stedile

et al.

International Journal of Peptide Research and Therapeutics, Journal Year: 2025, Volume and Issue: 31(2)

Published: Jan. 6, 2025

Language: Английский

Citations

0
177Lu-Labeled Heterodimeric Agent with High Stability Targeting Neovascularization for Tumor Radioligand Therapy DOI

Biao Yang,

Changyu Shan,

Xiaoying Lv

et al.

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Radiopharmaceutical theranostics holds significant promise in tumor diagnosis and treatment, but suboptimal uptake retention remain a persistent limitation. We have conjugated unique albumin binder to our previously developed heterodimeric precursor HX01 achieved novel L6, aiming prolong circulation time enhance accumulation retention. However, we observed that the NGR sequence of L6 was gradually rearranged iso-DGR under alkaline conditions, resulting decreased stability. In this study, further modified synthesize XH02, subsequently assessing their vitro vivo properties following radiolabeling. Utilizing positron emission tomography (PET)/computed (CT) imaging, single-photon computed (SPECT)/CT biodistribution study BxPC-3 xenograft mice, striking radiopharmaceutical within tumors. Two cycles administration 177Lu-XH02 displayed exceptional growth inhibition tumors while causing minimal side effects. This promising result underscores immense potential agent for clinical translation investigation.

Language: Английский

Citations

0
From Canonical to Unique: Extension of A Lipophilicity Scale of Amino Acids to Non-Standard Residues DOI Creative Commons
Antonio Viayna,

Paulina Matamoros,

David Blázquez-Ruano

et al.

Published: Jan. 31, 2024

The lipophilicity of amino acids plays a pivotal role in determining their physicochemical properties as it gives an estimate solubility, binding propensity, and bioavailability. Herein, we applied the IEFPCM/MST implicit solvation model to compute n-octanol/water partition coefficient lipophilic descriptor for non-standard acids. Thus, extending our previous work on hydrophobicity scale To this end, employed two structural models, named Model 1 2, differentiated solely by C-terminal capping groups using N- or O- methyl substituent, respectively. Our findings revealed substantial similarities between validating values side chains. Differences were observed fewer cases, indicating effect group chain hydrophobicity. This is expected one contains hydrogen bond donor (Model 1) while other uses acceptor 2). Overall, both models exhibit strong correlations with experimental values, showing lower statistical errors. In addition, predictions able correctly predict change due number acetylated lysines peptide pairs determined HPLC, suggesting that can be proteomics studies include post-translational modifications beyond acetylation.

Language: Английский

Citations

2
Conductometric investigation of nano MnSO4 association parameters and complex formation with glycylglycine in aqueous and methanol-water mixtures: Thermodynamic parameters and potential applications DOI

Radwa Rashad,

Hany Nasef, Esam A. Gomaa

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 267, P. 131648 - 131648

Published: April 15, 2024

Language: Английский

Citations

1
Lipid-Based Nanocarrier by Targeting with LHRH Peptide: A Promising Approach for Prostate Cancer Radio-Imaging and Therapy DOI
Kakali De, S. K. Eashayan Tanbir, Samarendu Sinha

et al.

Molecular Pharmaceutics, Journal Year: 2024, Volume and Issue: unknown

Published: June 26, 2024

Prostate cancer is a prevalently detected malignancy with dismal prognosis. Luteinizing-hormone-releasing-hormone (LHRH) receptors are overexpressed in such cells, to which the LHRH-decapeptide can specifically bind. A lipid-polyethylene glycol-conjugated new analogue (D-P-HLH) was synthesized and characterized. D-P-HLH-coated anticancer drug doxorubicin (DX)-loaded solid lipid nanoparticles (F-DX-SLN) were formulated by cold homogenization technique characterized Fourier transform infrared spectroscopy, X-ray diffraction, photoelectron differential scanning calorimetry, dynamic light scattering, electron microscopy, entrapment efficiency, drug-release profile studies. F-DX-SLN allows site-specific DX delivery reducing side effects of chemotherapy. Cancer cells could precisely take up targeting specific receptors, boosting cytotoxicity at tumor site. The efficacy on PC3/SKBR3 MTT assay revealed that more cytotoxic than and/or DX-SLN. Flow cytometry confocal microscopic studies further support F-DX-SLNs' increased intracellular absorption capability LHRH cells. ensured high apoptotic potential, noticeably larger mitochondrial transmembrane depolarization action, as well activation caspases, longer half-life, greater plasma concentration. F-DX-SLN/DX-SLN radiolabeled technetium-99m; scintigraphic imaging established its selectivity PC3 tumor-bearing nude mice. formulations treatment, vivo therapeutic tests, histopathological also conducted. Results clearly indicate exhibits sustained superior targeted administration drugs, thus opening possibility system precise control effects. provide nanotheranostic approach improved for prostate therapy.

Language: Английский

Citations

1
Integrating Computational Design and Experimental Approaches for Next-Generation Biologics DOI Open Access

Ahrum Son,

Jongham Park, Woojin Kim

et al.

Published: July 24, 2024

Therapeutic protein engineering has revolutionized medicine by enabling the development of highly specific and potent treatments for a wide range diseases. This review examines recent advances in computational experimental approaches improved therapeutics. Key areas focus include antibody engineering, enzyme replacement therapies, cytokine-based drugs. Computational methods like structure-based design, machine learning integration, language models have dramatically enhanced our ability to predict properties guide efforts. Experimental techniques such as directed evolution rational design continue evolve, with high-throughput accelerating discovery process. Applications these led breakthroughs affinity maturation, bispecific antibodies, stability enhancement, conditionally active cytokines. Emerging intracellular delivery, stimulus-responsive proteins, de novo designed therapeutic proteins offer exciting new possibilities. However, challenges remain predicting vivo behavior, scalable manufacturing, immunogenicity mitigation, targeted delivery. Addressing will require continued integration methods, well deeper understanding behavior complex physiological environments. As field advances, we can anticipate increasingly sophisticated effective therapeutics treating human

Language: Английский

Citations

1
Integrating Computational Design and Experimental Approaches for Next-Generation Biologics DOI Creative Commons

Ahrum Son,

Jongham Park, Woojin Kim

et al.

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1073 - 1073

Published: Aug. 27, 2024

Therapeutic protein engineering has revolutionized medicine by enabling the development of highly specific and potent treatments for a wide range diseases. This review examines recent advances in computational experimental approaches improved therapeutics. Key areas focus include antibody engineering, enzyme replacement therapies, cytokine-based drugs. Computational methods like structure-based design, machine learning integration, language models have dramatically enhanced our ability to predict properties guide efforts. Experimental techniques such as directed evolution rational design continue evolve, with high-throughput accelerating discovery process. Applications these led breakthroughs affinity maturation, bispecific antibodies, stability enhancement, conditionally active cytokines. Emerging intracellular delivery, stimulus-responsive proteins, de novo designed therapeutic proteins offer exciting new possibilities. However, challenges remain predicting vivo behavior, scalable manufacturing, immunogenicity mitigation, targeted delivery. Addressing will require continued integration methods, well deeper understanding behavior complex physiological environments. As field advances, we can anticipate increasingly sophisticated effective therapeutics treating human

Language: Английский

Citations

1