Real-time Optical Tracking of Protein Corona Formation on Single Nanoparticles in Serum DOI Creative Commons
Mathias Dolci, Yuyang Wang, Sjoerd W. Nooteboom

et al.

Published: June 7, 2023

Significant advances in synthesis and functionalization have provided state-of-the-art technology controlling the physico-chemical properties of nanomaterials. These are finding numerous applications including biomedical field whereby nanoparticles injected vivo for medical imaging, theranostics biosensing. However, interactions with proteins contained biological fluids lead to formation a shell on surface called "protein corona" (PC). PC plays detrimental role intended as it may modify interface nanoparticle thereby block functional groups needed recognition. It is therefore essential understand mechanisms these PCs order control chemistry complex fluids. Current characterization techniques can identify quantify composition using mass spectroscopy electrophoresis. most them do not enable real-time measurement media because they require washing steps remove excess protein. Finally, provide ensemble averages unable access inter-particle heterogeneity. Here, we demonstrate use single-particle scattering microscopy combined microfluidic system study at single-nanoparticle level. The method label-free operates undiluted blood serum. We probe both, metallic dielectric different chemistries. Analysis protein adsorption revealed unexpectedly strong heterogeneity amount accumulated varies by up factor 10 between particles. Furthermore, found that roughness affects kinetics formation. results this in-situ powerful tool optimize minimize thus increase efficiency such targeted drug delivery.

Language: Английский

The role of protein corona on nanodrugs for organ-targeting and its prospects of application DOI
Sijie Wang, Junlei Zhang, Huan‐Li Zhou

et al.

Journal of Controlled Release, Journal Year: 2023, Volume and Issue: 360, P. 15 - 43

Published: June 20, 2023

Language: Английский

Citations

35
Accelerating therapeutic protein design with computational approaches toward the clinical stage DOI Creative Commons
Zhidong Chen, Xinpei Wang, Xu Chen

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21, P. 2909 - 2926

Published: Jan. 1, 2023

Therapeutic protein, represented by antibodies, is of increasing interest in human medicine. However, clinical translation therapeutic protein still largely hindered different aspects developability, including affinity and selectivity, stability aggregation prevention, solubility viscosity reduction, deimmunization. Conventional optimization the developability with widely used methods, like display technologies library screening approaches, a time cost-intensive endeavor, efficiency finding suitable solutions not enough to meet needs. In recent years, accelerated advancement computational methodologies has ushered transformative era field design. Owing their remarkable capabilities feature extraction modeling, integration cutting-edge strategies conventional techniques presents promising avenue accelerate progression design toward implementation. Here, we compared differences between small molecules provided an overview approaches applicable or several issues.

Language: Английский

Citations

22
Real-Time Optical Tracking of Protein Corona Formation on Single Nanoparticles in Serum DOI Creative Commons
Mathias Dolci, Yuyang Wang, Sjoerd W. Nooteboom

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(20), P. 20167 - 20178

Published: Oct. 6, 2023

The formation of a protein corona, where proteins spontaneously adhere to the surface nanomaterials in biological environments, leads changes their physicochemical properties and subsequently affects intended biomedical functionalities. Most current methods study corona are ensemble-averaging either require fluorescent labeling, washing steps, or only applicable specific types particles. Here we introduce real-time all-optical nanoparticle analysis by scattering microscopy (RONAS) track full serum, at single-particle level, without any labeling. RONAS uses optical enables situ tracking adsorption on metallic dielectric nanoparticles with different geometries directly blood serum. We analyzed adsorbed mass, affinity, kinetics single particle level. While there is high degree heterogeneity from particle, predominant factor chemistry rather than underlying material size. offers an in-depth understanding mechanisms related coronas and, thus, development strategies engineer efficient bionanomaterials.

Language: Английский

Citations

21
Translational Challenges and Prospective Solutions in the Implementation of Biomimetic Delivery Systems DOI Creative Commons
Zhe Wang,

Xinpei Wang,

Wanting Xu

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(11), P. 2623 - 2623

Published: Nov. 14, 2023

Biomimetic delivery systems (BDSs), inspired by the intricate designs of biological systems, have emerged as a groundbreaking paradigm in nanomedicine, offering unparalleled advantages therapeutic delivery. These encompassing platforms such liposomes, protein-based nanoparticles, extracellular vesicles, and polysaccharides, are lauded for their targeted delivery, minimized side effects, enhanced outcomes. However, translation BDSs from research settings to clinical applications is fraught with challenges, including reproducibility concerns, physiological stability, rigorous efficacy safety evaluations. Furthermore, innovative nature demands reevaluation evolution existing regulatory ethical frameworks. This review provides an overview delves into multifaceted translational challenges present emerging solutions, underscored real-world case studies. Emphasizing potential redefine healthcare, we advocate sustained interdisciplinary collaboration research. As our understanding deepens, future appears promising, focus on personalized medicine refined patient-specific systems.

Language: Английский

Citations

20
Polymeric Nanoparticles and Nanogels: How Do They Interact with Proteins? DOI Creative Commons

Amirhossein Sadeghi,

Shadi PourEskandar,

Esfandyar Askari

et al.

Gels, Journal Year: 2023, Volume and Issue: 9(8), P. 632 - 632

Published: Aug. 6, 2023

Polymeric nanomaterials, nanogels, and solid nanoparticles can be fabricated using single or double emulsion methods. These materials hold great promise for various biomedical applications due to their biocompatibility, biodegradability, ability control interactions with body fluids cells. Despite the increasing use of in biomedicine plethora publications on topic, biological behavior efficacy polymeric (PNPs) have not been as extensively studied those other nanoparticles. The gap between potential PNPs mainly attributed incomplete understanding identity. Under physiological conditions, such specific temperatures adequate protein concentrations, become coated a “protein corona” (PC), rendering them potent tools proteomics studies. In this review, we initially investigate synthesis routes chemical composition conventional better comprehend how they interact proteins. Subsequently, comprehensively explore effects material parameters proteins, encompassing reactions hydrophobic bonding electrostatic interactions. Moreover, delve into recent advances PNP-based models that applied nanoproteomics, discussing new opportunities offer clinical translation early prediction diseases. By addressing these essential aspects, aim shed light foster further research critical area.

Language: Английский

Citations

16
Protein Aggregation on Metal Oxides Governs Catalytic Activity and Cellular Uptake DOI Creative Commons
Robert Nißler,

Lena Dennebouy,

Alexander Gogos

et al.

Small, Journal Year: 2024, Volume and Issue: 20(34)

Published: March 31, 2024

Abstract Engineering of catalytically active inorganic nanomaterials holds promising prospects for biomedicine. Catalytically metal oxides show applications in enhancing wound healing but have also been employed to induce cell death photodynamic or radiation therapy. Upon introduction into a biological system, are exposed complex fluids, causing interaction and adsorption ions proteins. While protein corona formation on is acknowledged, its modulation nanomaterial catalytic efficacy less understood. In this study, proteomic analyses nano‐analytic methodologies quantify characterize adsorbed proteins, correlating layer with oxide activity vitro vivo. The comprises up 280 different constituting 38% by weight. Enhanced complement factors other opsonins nanocatalyst surfaces lead their uptake macrophages when applied topically, localizing >99% the tissue‐resident macrophages. Initially, significantly reduces nanocatalysts' activity, can be partially recovered endosomal conditions due proteolytic degradation corona. Overall, research reveals relationship between physisorbed proteins characteristics specific nanoparticles, providing design parameters optimizing nanocatalysts environments.

Language: Английский

Citations

5
Erythrocyte−cancer hybrid membrane-coated reduction-sensitive nanoparticles for enhancing chemotherapy efficacy in breast cancer DOI Creative Commons
Somayeh Rezaei, Raimundo Fernandes de Araújo Júnior,

Isadora Luisa Gomes da Silva

et al.

Biomaterials Advances, Journal Year: 2023, Volume and Issue: 151, P. 213456 - 213456

Published: May 10, 2023

Cell-membrane-coated biomimetic nanoparticles (NPs) have attracted great attention due to their prolonged circulation time, immune escape mechanisms and homotypic targeting properties. Biomimetic nanosystems from different types of cell -membranes (CMs) can perform increasingly complex tasks in dynamic biological environments thanks specific proteins other properties inherited the source cells. Herein, we coated doxorubicin (DOX)-loaded reduction-sensitive chitosan (CS) NPs with 4T1 cancer (CCMs), red blood (RBCMs) hybrid erythrocyte-cancer membranes (RBC-4T1CMs) enhance delivery DOX breast The physicochemical (size, zeta potential morphology) resulting RBC@DOX/CS-NPs, 4T1@DOX/CS-NPs RBC-4T1@DOX/CS-NPs, as well cytotoxic effect cellular NP uptake vitro were thoroughly characterized. anti-cancer therapeutic efficacy was evaluated using orthotopic model vivo. experimental results showed that DOX/CS-NPs had a DOX-loading capacity 71.76 ± 0.87 %, coating 4T1CM significantly increased Interestingly, by optimizing ratio RBCMs:4T1CMs, it possible increase towards Moreover, vivo tumor studies compared control free DOX, both RBC@DOX/CS-NPs inhibited growth metastasis. However, more prominent. CM-coating reduced macrophages led rapid clearance liver lungs vivo, NPs. Our suggest self-recognition cells In conclusion, tumor-disguised CM-coated exhibited properties, superior over RBC-CM or RBC-4T1 membranes, suggesting presence 4T1-CM is critical for treatment outcome.

Language: Английский

Citations

13
Immune response recalibration using immune therapy and biomimetic nano-therapy against high-grade gliomas and brain metastases DOI Creative Commons

Puja Sandhbor,

Geofrey John,

Sakshi Bhat

et al.

Asian Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 101021 - 101021

Published: Jan. 1, 2025

Although with aggressive standards of care like surgical resection, chemotherapy, and radiation, high-grade gliomas (HGGs) brain metastases (BM) treatment has remained challenging for more than two decades. However, technological advances in this field immunotherapeutic strategies have revolutionized the HGGs BM. Immunotherapies immune checkpoint inhibitors, CAR-T targeting, oncolytic virus-based therapy, bispecific antibody treatment, vaccination approaches, etc., are emerging as promising avenues offering new hope refining patient's survival benefits. selective trafficking across blood-brain barrier (BBB), immunosuppressive tumor microenvironment (TME), metabolic alteration, heterogeneity limit therapeutic efficacy immunotherapy Furthermore, to address concern, NanoBioTechnology-based bioinspired delivery system been gaining tremendous attention recent years. With such Trojan horse targeting infusing/camouflaging nanoparticles surface biological molecules/cells immunocytes, erythrocytes, platelets, glioma cell lysate and/or integrating these get hybrid membrane homotypic recognition. These biomimetic nanotherapy offers advantages over conventional nanoparticles, focusing on greater target specificity, increased circulation stability, higher active loading capacity, BBB permeability (inherent inflammatory chemotaxis neutrophils), decreased immunogenicity, efficient metabolism-based combinatorial effects, prevention recurrence by induction immunological memory, etc. provide age improved immunotherapies outcomes against In review, we emphasize neuro-immunotherapy versatility nano-delivery precise hard-to-treat most lethal Moreover, challenges impeding clinical translatability approaches were addressed unmet medical needs cancers.

Language: Английский

Citations

0
Understanding nanoscale transport in confined spaces: Protecting encapsulated guests with metal-organic frameworks DOI
Abdelkarim Chaouiki,

Maryam Chafiq,

Noureddine Elboughdiri

et al.

Applied Materials Today, Journal Year: 2025, Volume and Issue: 44, P. 102676 - 102676

Published: March 15, 2025

Language: Английский

Citations

0
Optimal delivery strategies for nanoparticle-mediated mRNA delivery DOI
Xiaoyan Li,

Xiaocui Guo,

Mingdi Hu

et al.

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 11(10), P. 2063 - 2077

Published: Jan. 1, 2023

Messenger RNA (mRNA) has emerged as a new and efficient agent for the treatment of various diseases. The success lipid nanoparticle-mRNA against novel coronavirus (SARS-CoV-2) pneumonia epidemic proved clinical potential formulations. However, deficiency in effective biological distribution, high transfection efficiency good biosafety are still major challenges translation nanomedicine mRNA delivery. To date, variety promising nanoparticles have been constructed then gradually optimized to facilitate biodistribution carriers In this review, we describe design with an emphasis on nanoparticles, discuss manipulation strategies nanoparticle-biology (nano-bio) interactions delivery overcome barriers improve efficiency, because specific nano-bio interaction usually remoulds biomedical physiological properties especially biodistribution, mechanism cellular internalization immune response. Finally, give perspective future applications technology. We believe that regulation would be significant breakthrough cross barriers. This review may provide direction nanoparticle-mediated systems.

Language: Английский

Citations

9