OX26-cojugated gangliosilated liposomes to improve the post-ischemic therapeutic effect of CDP-choline DOI Creative Commons
Nicola d’Avanzo, Donatella Paolino, Antonella Barone

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 2, 2024

Abstract Cerebrovascular impairment still represents one of the main causes death worldwide with a mortality rate 5.5 million per year. Furthermore, disability 50% surviving patients factor both high social impact and costs for long periods time national healthcare systems. For these reasons, efficacious clinical treatment suffering brain ischemic stroke is medical need. To this aim, liposome nanomedicine having monosialic ganglioside type 1 (GM1) between its constituent bearing OX26 (an anti-transferrin receptor antibody) was prepared by entrapping CDP-choline (a neurotrophic drug) (CDP-choline/OX26Lip), characterized tested in vivo on an rat model. CDP-choline/OX26Lip were freeze thaw followed extrusion through polycarbonate filters, thus achieving ~80 nm mean size homogeneous distribution. It demonstrated that showed suitable stability presence human serum. also pharmacokinetic profile, 30.0±4.2 % administered dose blood stream 12 h after systemic administration. The post-ischemic therapeutic effect better than CDP-choline/Lip, showing significant greater survival re-perfused rats, i.e. 96% 78% 8 days, respectively. significantly decreased peroxidation almost 5-fold compared to as expressed amount conjugated dienes,i.e. 13.9 ± 1.1 3.1 0.8 mmol/mg proteins, increased could be attributed improved accumulation encapsulated delivered OX26-conjugated GM1-liposomes. Therefore, may represent strategy reassessment line option events caused stroke, responding needs.

Language: Английский

Enzyme-Mediated Bioorthogonal Cascade Catalytic Reaction for Metabolism Intervention and Enhanced Ferroptosis on Neuroblastoma DOI
Qi Wang, Xiangze Li,

Zhiyao Cao

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(12), P. 8228 - 8241

Published: March 12, 2024

It remains a tremendous challenge to explore effective therapeutic modalities against neuroblastoma, lethal cancer of the sympathetic nervous system with poor prognosis and disappointing treatment outcomes. Considering limitations conventional intrinsic vulnerability we herein develop pioneering sequential catalytic that utilizes lactate oxidase (LOx)/horseradish peroxidase (HRP)-loaded amorphous zinc metal–organic framework, named LOx/HRP-aZIF, in combination 3-indole-acetic acid (IAA) prodrug. On basis abnormal accumulation occurs tumor microenvironment, cascade reaction LOx HRP consumes endogenous glutathione reduced form nicotinamide adenine dinucleotide achieve first stage killing cells via antioxidative incapacitation electron transport chain interference. Furthermore, generation reactive oxygen species induced by IAA through bioorthogonal catalysis promotes ferritin degradation lipid peroxidation, ultimately provoking self-enhanced ferroptosis positive feedback initiating an Fenton reaction. This work highlights superiority natural enzyme-dependent reaction, offering paradigm for synergistically enzyme-based metabolism-ferroptosis anticancer therapy.

Language: Английский

Citations

10
Nanoplasmonics for Enhanced Fluorescence Detection of Nucleic Acids: From Fundamentals to Boosting Cancer Management DOI Creative Commons
David Botequim, Rui Oliveira‐Silva, Vanda Vaz Serra

et al.

Advanced NanoBiomed Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Cancer remains a leading cause of mortality and morbidity worldwide. Consequently, the scientific community continues to pursue improvements in diagnostic methods subsequent treatments. To enhance treatment efficacy reduce probability adverse outcomes, reliable sensitive are essential. A potential solution may lie synergy between nanotechnology optical biosensors, as they can provide exceptional sensitivity detection disease biomarkers. This review focuses on fluorescent DNA probes assembled onto metal nanoparticles for cancer‐related applications. These hybrid biosensors exhibit remarkable versatile properties enabling signal emission by orders magnitude. In these configurations, metallic particles function antennas dyes, significantly increasing their photon rates. presents novel perspective recent advancements cancer diagnostics utilizing biosensors. Current methodologies examined, toward elucidating advantages limitations. The subject matter is critically evaluated, fundamental concepts explored assess most promising avenues clinical potentially integrate into medical practice healthcare near future, both diagnosis prognosis cancer, well precision (P4) medicine.

Language: Английский

Citations

1
Splenic B cell-targeting lipid nanoparticles for safe and effective mRNA vaccine delivery DOI Creative Commons
Yuichi Suzuki,

Mai Yakuwa,

Mina Sato

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113687 - 113687

Published: April 1, 2025

Language: Английский

Citations

1
Real-Time Optical Tracking of Protein Corona Formation on Single Nanoparticles in Serum DOI Creative Commons
Mathias Dolci, Yuyang Wang, Sjoerd W. Nooteboom

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(20), P. 20167 - 20178

Published: Oct. 6, 2023

The formation of a protein corona, where proteins spontaneously adhere to the surface nanomaterials in biological environments, leads changes their physicochemical properties and subsequently affects intended biomedical functionalities. Most current methods study corona are ensemble-averaging either require fluorescent labeling, washing steps, or only applicable specific types particles. Here we introduce real-time all-optical nanoparticle analysis by scattering microscopy (RONAS) track full serum, at single-particle level, without any labeling. RONAS uses optical enables situ tracking adsorption on metallic dielectric nanoparticles with different geometries directly blood serum. We analyzed adsorbed mass, affinity, kinetics single particle level. While there is high degree heterogeneity from particle, predominant factor chemistry rather than underlying material size. offers an in-depth understanding mechanisms related coronas and, thus, development strategies engineer efficient bionanomaterials.

Language: Английский

Citations

21
The biomolecular corona of nanomedicines: effects on nanomedicine outcomes and emerging opportunities DOI Creative Commons
Anna Salvati

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103101 - 103101

Published: March 11, 2024

Upon administration, nanomedicines adsorb a corona of endogenous biomolecules on their surface, which can affect nanomedicine interactions with cells, targeting, and efficacy. While strategies to reduce protein binding are available, the high selectivity adsorbed is enabling novel applications, such as for biomarker discovery rare identification. Additionally, molecules promote specific cell receptors, thus conferring new targeting capabilities. This has been reported Onpattro, lipid nanoparticle hepatocytes via apolipoproteins in its corona. Recently, selective organ-targeting (SORT) nanoparticles have proposed, exploit corona-mediated deliver outside liver. Strategies seeding engineering emerging increase similar mechanisms.

Language: Английский

Citations

8
OX26-cojugated gangliosilated liposomes to improve the post-ischemic therapeutic effect of CDP-choline DOI Creative Commons
Nicola d’Avanzo, Donatella Paolino, Antonella Barone

et al.

Drug Delivery and Translational Research, Journal Year: 2024, Volume and Issue: 14(10), P. 2771 - 2787

Published: March 13, 2024

Abstract Cerebrovascular impairment represents one of the main causes death worldwide with a mortality rate 5.5 million per year. The disability 50% surviving patients has high social impacts and costs in long period treatment for national healthcare systems. For these reasons, efficacious clinical patients, brain ischemic stroke, remains medical need. To this aim, liposome nanomedicine, monosialic ganglioside type 1 (GM1), OX26 (an anti-transferrin receptor antibody), CDP-choline (a neurotrophic drug) (CDP-choline/OX26Lip) was prepared. CDP-choline/OX26Lip were prepared by freeze thaw method then extruded through polycarbonate filters, to have narrow size distributed liposomes ~80 nm. stable human serum, they had suitable pharmacokinetic properties, 30.0 ± 4.2% injected drug still present blood stream 12 h after its systemic injection. post-ischemic therapeutic effect is higher than CDP-choline/Lip, thus showing significantly survival re-perfused rats, i.e. 96% 78% 8 days. decreased peroxidation ~5-times compared CDP-choline/Lip; resulting conjugated dienes, that 13.9 1.1 mmol/mg proteins CDP-choline/Lip 3.1 0.8 CDP-choline/OX26Lip. increased accumulation GM1-liposomes tissues CDP-choline. Therefore, nanomedicine may represent strategy reassessment treat events caused respond significant Graphical abstract

Language: Английский

Citations

7
The nanoparticle-Protein Corona untold history (1907–2007) DOI
Eudald Casals, Michele Vitali, Víctor Puntes

et al.

Nano Today, Journal Year: 2024, Volume and Issue: 58, P. 102435 - 102435

Published: Aug. 5, 2024

Language: Английский

Citations

7
In situ characterization techniques of protein corona around nanomaterials DOI Creative Commons

Fangqin Fu,

Daniel Crespy, Katharina Landfester

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

We discuss here the in situ characterization methods for unraveling nanoparticle–protein interactions, highlighting challenges of protein corona and its significance nanomedicine development clinical translation.

Language: Английский

Citations

7
Navigating Neurotoxicity and Safety Assessment of Nanocarriers for Brain Delivery: Evaluation Models and Insights DOI Creative Commons
Maria Inês Teixeira, Carla M. Lopes, Maria Helena Amaral

et al.

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 189, P. 25 - 56

Published: Sept. 20, 2024

Language: Английский

Citations

5
Dual-Purpose Aptamer-Based Sensors for Real-Time, Multiplexable Monitoring of Metabolites in Cell Culture Media DOI
Yiling Yang, Xumei Gao,

Bryce Widdicombe

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 10, 2024

The availability of high-frequency, real-time measurements the concentrations specific metabolites in cell culture systems will enable a deeper understanding cellular metabolism and facilitate application good laboratory practice standards protocols. However, currently available approaches to this end either are constrained single-time-point single-parameter or limited range detectable analytes. Electrochemical aptamer-based (EAB) biosensors have demonstrated utility monitoring analytes

Language: Английский

Citations

4