Electrochemiluminescence biosensors for detection of cancer biomarkers in biofluids: Principles, opportunities, and challenges

Nano Today, Journal Year: 2023, Volume and Issue: 50, P. 101874 - 101874

Published: May 18, 2023

Language: Английский

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Recent advances in nanomaterial-based optical biosensors for food safety applications: Ochratoxin-A detection, as case study

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(18), P. 6318 - 6360

Published: Jan. 23, 2023

Global population growth tremendously impacts the global food industry, endangering safety and quality. Mycotoxins, particularly Ochratoxin-A (OTA), emerge as a chain production threat, since it is produced by fungus that contaminates different species products. Beyond this, OTA exhibits possible human toxicological risk can lead to carcinogenic neurological diseases. A selective, sensitive, reliable biodetection approach essential ensure safety. Current detection approaches rely on accurate time-consuming laboratory techniques performed at end of process, or lateral-flow technologies are rapid on-site, but do not provide quantitative precise concentration measurements. Nanoengineered optical biosensors arise an avant-garde solution, providing high sensing performance, fast screening, which attractive for industrial market. This review core presents discusses recent advancements in biosensing, considering engineered nanomaterials, transduction principle biorecognition methodologies. Finally, major challenges future trends discussed, current patented emphasized particular promising method.

Language: Английский

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Uncovering the Depths of the Human Proteome: Antibody-based Technologies for Ultrasensitive Multiplexed Protein Detection and Quantification

Molecular & Cellular Proteomics, Journal Year: 2021, Volume and Issue: 20, P. 100155 - 100155

Published: Jan. 1, 2021

Probing the human proteome in tissues and biofluids such as plasma is attractive for biomarker drug target discovery. Recent breakthroughs multiplex, antibody-based, proteomics technologies now enable simultaneous quantification of thousands proteins at low sub fg/ml concentrations with remarkable dynamic ranges up to 10-log. We herein provide a comprehensive guide methodologies, performance, technical comparisons, advantages, disadvantages established emerging multiplexed ultrasensitive measurement proteins. Gaining holistic knowledge on these innovations crucial choosing right tool applications hand critically complement traditional methods. This can bring researchers closer than ever before elucidating intricate inner workings cross talk that spans multitude disease mechanisms.

Language: Английский

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Recent developments and fabrication of the different electrochemical biosensors based on modified screen printed and glassy carbon electrodes for the early diagnosis of diverse breast cancer biomarkers

Materials Today Chemistry, Journal Year: 2022, Volume and Issue: 26, P. 101129 - 101129

Published: Sept. 6, 2022

Language: Английский

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Recent advances of nucleic acid-based cancer biomarkers and biosensors

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 497, P. 215456 - 215456

Published: Sept. 26, 2023

Language: Английский

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Ultrasensitive Protein Detection Technologies for Extracellular Vesicle Measurements

Molecular & Cellular Proteomics, Journal Year: 2023, Volume and Issue: 22(6), P. 100557 - 100557

Published: April 23, 2023

Extracellular vesicles (EVs) are nanoscopic, heterogenous, lipid-rich particles that carry a multitude of cargo biomolecules including proteins, nucleic acids, and metabolites. Although historically EVs were regarded as cellular debris with no intrinsic value, growing understanding EV biogenesis has led to the realization facilitate intercellular communication sources liquid biomarkers. can be isolated analyzed from wide variety accessible biofluids for biomarker discovery diagnostic applications. There is diversity different biological compartments (e.g., cells tissues), some these present at extremely low concentrations. Consequently, challenge in field find appropriate markers enable selective isolation rare EVs. Many conventional protein detection technologies have limited sensitivity detect abundance biomarkers EVs, limiting their use research. Advances ultrasensitive needed harness potential clinical application. This Perspective highlights current research focusing on technologies, limitations, areas growth future.

Language: Английский

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Application of Nanobiosensor Engineering in the Diagnosis of Neurodegenerative Disorders

Results in Engineering, Journal Year: 2024, Volume and Issue: 24, P. 102790 - 102790

Published: Sept. 5, 2024

Language: Английский

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Electrochemical Determination of Chlorogenic Acid in Nutraceuticals Using Voltammetric Sensors Based on Screen-Printed Carbon Electrode Modified with Graphene and Gold Nanoparticles

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(16), P. 8897 - 8897

Published: Aug. 18, 2021

The present study describes the electrochemical properties of three screen-printed electrodes (SPEs), first electrode being carbon-based (C), second graphene-based (GPH), and third based on GPH modified with gold nanoparticles (GNP). These were used for behavior chlorogenic acid in different aqueous solutions, at pH = 7. In solution, a redox process was noticed case all electrodes; GNP significantly improved sensor response regarding sensitivity reversibility, fact demonstrated by characterizing cyclic voltammetry potassium ferrocyanide, which corresponds to exchange two electrons protons. Moreover, calibration curves each developed, subsequently calculating detection limits (LOD) quantification (LOQ). Low LOD LOQ obtained, best—of order 10−7 M (LOD 0.62 × M; 1.97 M)—being obtained GPH-GNP-SPE, demonstrates that method may be applied determining real samples. Thus, sensors successfully quantitative determination nutraceutical products. validation results done using FTIR method. accordance those spectrometric method, without significant differences from statistical point view.

Language: Английский

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Carbon nanodot–based electrogenerated chemiluminescence biosensor for miRNA-21 detection

Microchimica Acta, Journal Year: 2021, Volume and Issue: 188(11)

Published: Oct. 30, 2021

A simple carbon nanodot-based electrogenerated chemiluminescence biosensor is described for sensitive and selective detection of microRNA-21 (miRNA-21), a biomarker several pathologies including cardiovascular diseases (CVDs). The photoluminescent nanodots (CNDs) were obtained using new synthesis method, simply by treating tiger nut milk in microwave reactor. environmentally friendly, simple, efficient. optical properties morphological characteristics the CNDs exhaustively investigated, confirming that they have oxygen nitrogen functional groups on their surfaces exhibit excitation-dependent fluorescence emission, as well photostability. They act co-reactant agents anodic electrochemiluminescence (ECL) [Ru(bpy)3]2+, producing different signals probe (single-stranded DNA) hybridized target (double-stranded DNA). These results paved way development ECL miRNA-21. This was developed immobilization thiolated oligonucleotide, fully complementary to miRNA-21 sequence, disposable gold electrode. with electrode surface, hybridization detected enhancement [Ru(bpy)3]2+/DNA signal CNDs. shows linear response concentration up 100.0 pM limit 0.721 fM. method does not require complex labeling steps, has rapid response. It successfully used detect directly serum samples from heart failure patients without previous RNA extraction neither amplification process.

Language: Английский

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Recent Advances in Nanomaterial‐based Optical Biosensors as Potential Point‐of‐Care Testing (PoCT) Probes in Carcinoembryonic Antigen Detection

Chemistry - An Asian Journal, Journal Year: 2022, Volume and Issue: 17(14)

Published: April 26, 2022

For the past decades, several cancer biomarkers have been exploited for rapid and accurate prognosis or diagnosis purposes. In this review, optical biosensor is targeted carcinoembryonic antigen (CEA) detection. The CEA level a prominent parameter currently used in clinical cases of cancer-related diseases. Many nanomaterial-based biosensors are invented as alternatives commonly enzyme-linked immunosorbent assays (ELISA) immunoassay method detection traditional approach. But they possess certain drawbacks such tedious procedure, high technical demand, costly. Nevertheless, effort appears to be wasted none them being actualised. Generally, sensor function was carried out by converting bio-signals generated upon interface receptor into light signals. These sensors were popular due specific advantages sensitivity, free from chemical electromagnetic interferences, wide dynamic range, easy monitored. features PoC diagnostics discussed associated various applications colorimetric-based chemiluminescent-based biosensors. roles nanomaterials each application also summarised comparing modification, incubation period, lowest limit (LOD) linear range amount. challenges future perspectives highlighted well at end review.

Language: Английский

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