Solid Electrochemiluminescence Sensor by Immobilization of Emitter Ruthenium(II)tris(bipyridine) in Bipolar Silica Nanochannel Film for Sensitive Detection of Oxalate in Serum and Urine

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(5), P. 390 - 390

Published: Feb. 20, 2024

Convenient and highly sensitive detection of oxalate ions in body fluids is crucial significance for disease prevention, diagnosis, monitoring treatment effectiveness. Establishing a simple solid-state electrochemiluminescence (ECL) sensing system desirable. In this work, solid ECL sensor was fabricated by immobilizing the commonly used emitter ruthenium(II)tris(bipyridine) (Ru(bpy)32+) on double-layered bipolar silica nanochannel array film (bp-SNA)-modified electrode, enabling serum or urine samples. Cost-effective readily available indium tin oxide (ITO) as supporting electrode. fabrication multiple negatively charged SNA (n-SNA)-modified ITO electrodes achieved through one-step Stöber solution growth method. Subsequently, positive outer layer (p-SNA) rapidly prepared using an electrochemical-assisted self-assembly The stable immobilization Ru(bpy)32+ electrode surface, facilitated electrostatic adsorption n-SNA repulsion p-SNA. Utilizing co-reactant Ru(bpy)32+, combined with enrichment p-SNA, constructed enabled ranging from 1 nM to 25 μM mM, limit (LOD) 0.8 nM. exhibited high selectivity good stability, making it suitable

Language: Английский

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Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 63 - 63

Published: Jan. 19, 2025

The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach fast and convenient biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains challenge. This study developed an immunosensor by engineering immunorecognition interface on outer surface mesoporous silica nanochannel film (SNF) confining Co3O4 nanocatalyst within SNF nanochannels to improve luminol efficiency. was grown indium tin oxide (ITO) electrode using simple Stöber solution growth method. A successfully confined through situ electrodeposition, confirmed X-ray photoelectron spectroscopy (XPS) electrochemical measurements. demonstrated excellent electrocatalytic activity, effectively enhancing H2O2 oxidation boosting signal conditions. Using interleukin-6 (IL-6) as proof-of-concept demonstration, epoxy functionalization enabled covalent immobilization capture antibodies, forming specific interface. IL-6 binding induced immunocomplex formation, which reduced allowed quantitative detection. showed linear range from 1 fg mL−1 10 ng mL−1, with limit (LOD) 0.64 mL−1. It also good selectivity anti-interference capabilities, enabling successful artificial GCF samples.

Language: Английский

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Homogeneous Aptasensor with Electrochemical and Electrochemiluminescence Dual Detection Channels Enabled by Nanochannel-Based Probe Enrichment and DNase I Cleavage for Tumor Biomarker Detection

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 746 - 746

Published: Feb. 6, 2025

Homogeneous aptasensors that eliminate the need for probe labeling or immobilization hold significant potential rapid detection of tumor biomarkers. Herein, a homogeneous aptasensor with electrochemical (EC) and electrochemiluminescence (ECL) dual channels was developed by integrating nanochannel-based enrichment DNase I cleavage selective biomarker, carbohydrate antigen 125 (CA125). A two-dimensional (2D) composite prepared assembling CA125-specific aptamer cationic tris(2,2′-bipyridyl)Ru(II) (Ru(bpy)32+), which exhibited both EC ECL properties, onto graphene oxide (GO) nanosheets (Ru(bpy)32+/Apt@GO). vertically ordered mesoporous silica film (VMSF) ultrasmall, uniform, aligned nanochannel arrays rapidly grown on inexpensive disposable indium tin (ITO) electrode, forming interface. Due to size exclusion effect ultrasmall nanochannels in VMSF, Ru(bpy)32+/Apt@GO unable penetrate nanochannels, resulting no detectable Ru(bpy)32+ signal electrode. Upon specific recognition CA125 aptamer, an aptamer-CA125 complex formed subsequently detached from GO. then cleaved complex, releasing allowing dissociate into solution. This enzymatic enabled re-enter binding cycle, amplifying release The electrostatic adsorption VMSF significantly enhanced signals. constructed linear range 0.1 U/mL 100 ng/mL, limit (LOD) 91 mU/mL. For detection, detected over 0.001 U/mL, LOD as low 0.4 demonstrated excellent selectivity successfully applied dual-mode EC/ECL fetal bovine serum samples.

Language: Английский

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Enhanced electrochemiluminescence of luminol at neutral medium using nanochannel-confined Co3O4 nanozyme for highly sensitive detection of tumor biomarker

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112903 - 112903

Published: Jan. 1, 2025

Language: Английский

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Homogeneous Electrochemical Aptamer Sensor Based on Two-Dimensional Nanocomposite Probe and Nanochannel Modified Electrode for Sensitive Detection of Carcinoembryonic Antigen

Molecules, Journal Year: 2023, Volume and Issue: 28(13), P. 5186 - 5186

Published: July 3, 2023

A rapid and convenient homogeneous aptamer sensor with high sensitivity is highly desirable for the electrochemical detection of tumor biomarkers. In this work, a demonstrated based on two-dimensional (2D) nanocomposite probe nanochannel modified electrode, which can realize sensitive carcinoembryonic antigen (CEA). Using π-π stacking electrostatic interaction, CEA (Apt) cationic redox (hexaammineruthenium(III), Ru(NH3)63+) are co-loaded graphite oxide (GO), leading to 2D (Ru(NH3)63+/Apt@GO). Vertically ordered mesoporous silica-nanochannel film (VMSF) easily grown supporting indium tin (ITO) electrode (VMSF/ITO) using assisted self-assembly (EASA) method within 10 s. The ultrasmall nanochannels VMSF exhibits enrichment towards Ru(NH3)63+ size exclusion material. When added in Ru(NH3)63+/Apt@GO solution, DNA recognizes binds releases be enriched detected by VMSF/ITO electrodes. Based mechanism, an ranging from 60 fg/mL 100 ng/mL limit (LOD) 14 fg/mL. Detection human serum also realized. constructed system does not require fixation recognitive surface or magnetic separation before detection, demonstrating potential applications rapid, sensing

Language: Английский

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Sensitive Electrochemical Detection of Carcinoembryonic Antigen Based on Biofunctionalized Nanochannel Modified Carbonaceous Electrode

Molecules, Journal Year: 2024, Volume and Issue: 29(4), P. 858 - 858

Published: Feb. 15, 2024

The convenient construction of carbon-based electrochemical immunosensors with high performance is highly desirable for the efficient detection tumor biomarkers. In this work, an immunosensor was fabricated by integrating a biofunctionalized mesoporous silica nanochannel film electrode, which can enable sensitive determination carcinoembryonic antigen (CEA) in serum. commonly used carbonaceous glassy carbon electrode (GCE), employed as supporting and pre-treated through polarization to achieve stable binding vertically ordered amino groups (NH2-VMSF) without use any adhesive layer. To fabricate immunorecognition interface, antibodies were covalently immobilized after on outer surface NH2-VMSF derivatized aldehyde groups. presence sites within high-density nanochannels facilitate migration negatively charged redox probes (Fe(CN)63-/4-) electrostatic adsorption, leading generation signals. CEA, formation immunocomplexes recognitive interface reduce signal Fe(CN)63-/4- electrode. Based principle, CEA achieved. be determined range from 0.01 ng mL−1 100 limit 6.3 pg mL−1. exhibited selectivity, fetal bovine serum

Language: Английский

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Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(9), P. 3276 - 3293

Published: Aug. 3, 2023

Electrochemical affinity biosensors are evolving at breakneck speed, strengthening and colonizing more niches drawing unimaginable roadmaps that increasingly make them protagonists of our daily lives. They achieve this by combining their intrinsic attributes with those acquired leveraging the significant advances occurred in (nano)materials technology, bio(nano)materials nature-inspired receptors, gene editing amplification technologies, signal detection processing techniques. The aim Perspective is to provide, support recent representative illustrative literature, an updated critical view repertoire opportunities, innovations, applications offered electrochemical fueled key alliances indicated. In addition, imminent challenges these biodevices must face new directions which they envisioned as players discussed.

Language: Английский

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Pronounced signal enhancement with gourd-shaped hollow PtCoNi bunched nanochains for electrochemical immunosensing of alpha-fetoprotein

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 422, P. 136608 - 136608

Published: Sept. 12, 2024

Language: Английский

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A highly sensitive immunosensor based on nanochannel-confined nano-gold enhanced electrochemiluminescence for procalcitonin detection

Frontiers in Chemistry, Journal Year: 2023, Volume and Issue: 11

Published: Oct. 9, 2023

Sensitive detection of procalcitonin (PCT) in serum is crucial for the timely diagnosis and treatment rheumatoid arthritis. In this work, an electrochemiluminescence (ECL) platform developed based on in-situ growth Au nanoparticles (AuNPs) nanochannels analyte-gated signal, which can realize ECL determination PCT with high sensitivity. Vertically ordered mesoporous silica films amine groups uniform nanochannel array (NH 2 -VMSF) easily grown supporting indium tin oxide (ITO) electrode through electrochemical assisted self-assembly method (EASA). Anchored by amino groups, AuNPs were within to catalyze generation reactive oxygen species (ROS) amplify signal luminol. An immuno-recognitive interface constructed outer surface NH -VMSF, covalent immobilization antibodies. presence PCT, immunocomplex will hinder diffusion luminol co-reactants, leading a gating effect decreased signals. Based principle, immunosensor detect range from 10 pg/mL 100 ng mL -1 limit (LOD) 7 pg . The also be used detecting serum. sensor has advantages simple fabrication sensitive detection, demonstrating great potential real sample analysis.

Language: Английский

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The Fabrication of a Probe-Integrated Electrochemiluminescence Aptasensor Based on Double-Layered Nanochannel Array with Opposite Charges for the Sensitive Determination of C-Reactive Protein

Molecules, Journal Year: 2023, Volume and Issue: 28(23), P. 7867 - 7867

Published: Nov. 30, 2023

The effective and sensitive detection of the important biomarker, C-reactive protein (CRP), is great significance in clinical diagnosis. development a convenient highly electrochemiluminescence (ECL) aptasensor with an immobilized emitter probe desirable. In this work, probe-integrated ECL aptamer sensor was constructed based on bipolar silica nanochannel film (bp-SNF) modified electrode for CRP. bp-SNF, ITO electrode, consisted dual-layered SNF film, including negatively charged inner (n-SNF) outer positive charge amino groups (p-SNF). emitter, tris(bipyridine) ruthenium (II) (Ru(bpy)32+), stably bp-SNF using dual electrostatic interactions n-SNF attracting p-SNF repelling. surface were aldehyde derivatized, allowing covalent immobilization recognitive aptamers (5′-NH2-CGAAGGGGATTCGAGGGGTGATTGCGTGCTCCATTTGGTG-3′), leading to recognition interface. When CRP bound interface, formed complex increased interface resistance reduced diffusion co-reactant tripropylamine (TPA) into nanochannels, decrease signal. Based mechanism, could achieve ranging from 0.01 1000 ng/mL, limit (DL) 8.5 pg/mL. method constructing simple, it offers high stability, good selectivity, sensitivity.

Language: Английский

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