Phytomedicine,
Journal Year:
2023,
Volume and Issue:
125, P. 155290 - 155290
Published: Dec. 20, 2023
In
our
previous
study,
we
provided
evidence
that
Astragalus
mongholicus
Bunge(AM)
and
its
extracts
possess
a
protective
capability
against
radiation-induced
damage,
potentially
mediated
through
the
reduction
of
reactive
oxygen
species
(ROS)
nitric
oxide
(NO).
However,
were
pleasantly
surprised
to
discover
during
experimentation
AM
not
only
offers
protection
radiation
damage
but
also
exhibits
sensitization
effect.
This
effect
may
be
attributed
specific
small
molecule
present
in
known
as
ononin.
Currently,
sensitizers
are
predominantly
found
nitrazole
drugs
nanomaterials,
with
no
existing
reports
on
properties
ononin,
nor
underlying
mechanism.
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
176, P. 116891 - 116891
Published: June 12, 2024
Ulcerative
colitis
(UC)
is
a
chronic
inflammatory
bowel
disease
primarily
affecting
the
mucosa
of
colon
and
rectum.
UC
characterized
by
recurrent
episodes,
often
necessitating
lifelong
medication
use,
imposing
significant
burden
on
patients.
Current
conventional
advanced
treatments
for
have
disadvantages
insufficient
efficiency,
susceptibility
to
drug
resistance,
notable
adverse
effects.
Therefore,
developing
effective
safe
drugs
has
become
an
urgent
need.
Autophagy
intracellular
degradation
process
that
plays
important
role
in
intestinal
homeostasis.
Emerging
evidence
suggests
aberrant
autophagy
involved
development
UC,
modulating
can
effectively
alleviate
experimental
colitis.
A
growing
number
studies
established
interplay
with
endoplasmic
reticulum
stress,
gut
microbiota,
apoptosis,
NLRP3
inflammasome,
all
which
contribute
pathogenesis
UC.
In
addition,
variety
epithelial
cells,
including
absorptive
goblet
Paneth
as
well
other
cell
types
like
neutrophils,
antigen-presenting
stem
cells
gut,
mediate
through
autophagy.
To
date,
many
found
natural
products
hold
potential
exert
therapeutic
effects
regulating
This
review
focuses
possible
pharmacological
mechanisms
target
recent
years,
aiming
provide
basis
new
development.
Journal of Biochemical and Molecular Toxicology,
Journal Year:
2024,
Volume and Issue:
38(6)
Published: May 21, 2024
Abstract
Cancer
is
one
of
the
major
causes
death
worldwide,
with
more
than
10
million
deaths
annually.
Despite
tremendous
advances
in
health
sciences,
cancer
continues
to
be
a
substantial
global
contributor
mortality.
The
current
treatment
methods
demand
paradigm
shift
that
not
only
improves
therapeutic
efficacy
but
also
minimizes
side
effects
conventional
medications.
Recently,
an
increased
interest
potential
natural
bioactive
compounds
several
types
has
been
observed.
Ononin,
referred
as
formononetin‐7‐O‐β‐d‐glucoside,
isoflavone
glycoside,
derived
from
roots,
stems,
and
rhizomes
various
plants.
It
exhibits
variety
pharmacological
effects,
including
Antiangiogenic,
anti‐inflammatory,
antiproliferative,
proapoptotic,
antimetastatic
activities.
review
presents
thorough
overview
sources,
chemistry,
pharmacokinetics,
role
ononin
affecting
mechanisms
involved
cancer.
discusses
synergistic
interactions
other
therapies.
combined
effect
methods.
Finally,
safety
studies,
comprising
both
vitro
vivo
assessments
ononin's
anticancer
activities,
are
described.
Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics,
Journal Year:
2024,
Volume and Issue:
32(5), P. 899 - 910
Published: Jan. 1, 2024
Osteosarcoma
is
a
very
serious
primary
bone
cancer
with
high
death
rate
and
dismal
prognosis.
Since
there
no
permanent
therapy
for
this
condition,
it
necessary
to
develop
cure.
Therefore,
investigation
was
carried
out
assess
the
impacts
biological
functions
of
hydroxysafflor
yellow
A
(HYSA)
in
osteosarcoma
cell
lines
(MG63).
In
investigational
study,
MG63
cells
were
utilized.
Microarray
experiments,
quantitative
polymerase
chain
reaction
(qPCR),
immunofluorescent
staining,
extracellular
acidification
(ECAR),
oxygen
consumption
(OCR),
glucose
consumption,
lactate
production,
ATP
levels,
proliferation
assay,
5-Ethynyl-2′-deoxyuridine
(EDU)
Western
blot
performed.
cells,
HYSA
lowered
metastasis
rates,
suppressed
EDU
number,
enhanced
caspase-3/9
activity
levels.
reduced
Warburg
effect
induced
ferroptosis
(FPT)
cells.
Inhibiting
diminished
HYSA's
anti-cancer
activities
The
stimulation
HIF-1α/SLC7A11
pathway
decreased
HIF-1α
one
target
spot
model
(OC).
altered
HIF-1α's
thermophoretic
activity;
following
binding
HYSA,
melting
point
increased
from
~55°C
~60°C.
significantly
thermal
stability
exogenous
WT
while
not
affecting
Mut
HIF-1α,
suggesting
that
ARG-311,
GLY-312,
GLN-347,
GLN-387
may
be
involved
interaction
between
HYSA.
Conclusively,
our
study
revealed
FPT
OC
through
mitochondrial
damage
by
HIF-1α/HK2/SLC7A11
pathway.
possible
therapeutic
option
or
other
cancers.
Cell Death and Disease,
Journal Year:
2024,
Volume and Issue:
15(5)
Published: May 30, 2024
Abstract
Osteosarcoma
(OS)
therapy
presents
numerous
challenges,
due
largely
to
a
low
survival
rate
following
metastasis
onset.
Nerve
growth
factor
(NGF)
has
been
implicated
in
the
and
progression
of
various
cancers;
however,
mechanism
by
which
NGF
promotes
osteosarcoma
yet
be
elucidated.
This
study
investigated
influence
on
migration
patients
(88
cases)
as
well
underlying
molecular
mechanisms,
based
RNA-sequencing
gene
expression
data
from
public
database
(TARGET-OS).
In
patients,
was
significantly
higher
than
that
other
factors.
observation
confirmed
bone
tissue
arrays
91
levels
matrix
metallopeptidase-2
(MMP-2)
protein
were
normal
bone,
strongly
correlated
with
tumor
stage.
summary,
is
positively
MMP-2
human
cell
upregulating
expression.
cellular
experiments
using
cells
(143B
MG63),
upregulated
promoted
wound
healing,
migration,
invasion.
Pre-treatment
MEK
ERK
inhibitors
or
siRNA
attenuated
effects
Stimulation
shown
promote
phosphorylation
along
MEK/ERK
signaling
pathway
decrease
microRNA-92a-1-5p
(miR-92a-1-5p).
vivo
involving
an
orthotopic
mouse
model,
overexpression
enhanced
lung
metastasis.
Note
larotrectinib
(a
tropomyosin
kinase
receptor)
inhibited
effect
conclusion,
it
appears
MMP-2-dependent
inhibiting
miR-92a-1-5p
via
cascade.
Larotrectinib
emerged
potential
drug
for
treatment
NGF-mediated
osteosarcoma.
World Journal of Surgical Oncology,
Journal Year:
2025,
Volume and Issue:
23(1)
Published: April 2, 2025
Osteosarcoma
is
a
common
bone
tumor
in
adolescents
and
children,
characterized
by
rapid
progression,
high
malignancy,
poor
prognosis,
tendency
for
pulmonary
metastasis.
Despite
extensive
research
efforts,
the
specific
driver
gene
associated
with
osteosarcoma
remains
unidentified,
underscoring
urgent
need
novel
therapeutic
targets
targeted
treatment
options.
In
vitro
studies
were
conducted
to
assess
effects
of
DCC-2036
on
proliferation,
migration,
invasion
(OS)
cell
lines,
employing
cloning
Transwell
experiments.
Network
pharmacological
analysis,
complemented
experimental
validation,
indicated
critical
target
responsible
inhibitory
DCC-2036.
RNA
sequencing
analysis
demonstrated
that
could
induce
autophagy
OS
cells,
relative
protein
levels
assessed
using
Western
blotting
following
inhibitor
3-MA
mTOR
agonist
MHY1485.
vivo
further
confirmed
role
proliferation
through
subcutaneous
tumorigenesis.
this
study,
we
small
molecule
tyrosine
kinase
effectively
inhibited
cells
both
cellular
animal
models.
We
found
significantly
suppressed
induced
apoptosis;
additionally,
it
notably
invasion,
epithelial-to-mesenchymal
transition
(EMT).
HCK
was
identified
as
key
mediating
osteosarcoma.
Mechanistically,
shown
inhibit
expression
phosphorylated
AKT
(p-AKT),
S6
(p-S6K),
4E-binding
1
(p-4EBP1)
within
downstream
PI3K/AKT/mTORC1
signaling
pathway.
Furthermore,
experiments
utilizing
xenografts
mice
growth
xenografted
143B
BALB/C-nude
mice.
Collectively,
these
findings
indicate
promotes
targeting
HCK/AKT/mTORC1
axis
exerts
anti-tumor
without
significant
toxicity.
Consequently,
emerges
promising
agent
HCK-overexpressing
International Journal of Molecular Sciences,
Journal Year:
2025,
Volume and Issue:
26(8), P. 3863 - 3863
Published: April 18, 2025
Osteosarcoma
(OS)
is
the
most
common
primary
bone
malignancy
in
children
and
adolescents.
Unfortunately,
drug
resistance
limits
efficacy
of
chemotherapeutic
treatment
compromises
therapeutic
outcomes
a
substantial
proportion
cases.
Aberrant
CpG
island
methylation-associated
transcriptional
silencing
contributes
to
chemoresistance
pediatric
solid
tumors.
Here,
using
whole-genome
DNA
methylation
screening
on
16
human
OS
specimens,
we
identify
receptor
interacting
protein
kinase-3
(RIPK3),
molecular
regulator
necroptosis
programmed
cell
death
pathway,
as
gene
target
aberrant
demonstrate
its
role
chemoresistance.
We
validated
these
findings
via
enforced
expression
DsiRNA
silencing,
evaluated
RIPK3
cisplatin
chemosensitivity
activation
through
MLKL
phosphorylation.
found
that
results
samples
lines.
Enforced
significantly
enhanced
cytotoxicity
cells
knockdown
reversed
cisplatin-sensitive
phenotype.
In
with
expression,
increased
phosphorylation
both
target,
MLKL,
indicative
induction
necroptosis.
an
important
mediator
potential
pharmacologic
improve
chemotherapy
drug-resistant
