Recent advances in and perspectives on binder materials for supercapacitors–A review

European Polymer Journal, Journal Year: 2024, Volume and Issue: 210, P. 112941 - 112941

Published: March 16, 2024

Language: Английский

---

Tailored Electrochemical Biosensor with poly-diallydimethylammonium chloride-functionalised multiwalled carbon nanotubes/gold nanoparticles/manganese dioxide, and Haemoglobin for Sensitive Hydrogen Peroxide Detection

Talanta, Journal Year: 2024, Volume and Issue: 276, P. 126290 - 126290

Published: May 19, 2024

Language: Английский

---

Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: May 4, 2022

Mining novel specific molecular targets and establishing efficient identification methods are significant for detecting Pseudomonas aeruginosa, which can enable P. aeruginosa tracing in food water. Pangenome analysis was used to analyze the whole genomic sequences of 2017 strains (including 1,000 1,017 other common foodborne pathogen strains) downloaded from gene databases obtain species-specific genes, yielding a total 11 such genes. Four target UCBPP-PA14_00095, UCBPP-PA14_03237, UCBPP-PA14_04976, UCBPP-PA14_03627, were selected use, had 100% coverage strain not present nontarget bacteria. PCR primers (PA1, PA2, PA3, PA4) qPCR (PA12, PA13, PA14, PA15) designed based on these genes establish detection methods. For primer set, minimum limit DNA 65.4 fg/μl, observed set PA2 UCBPP-PA14_03237 gene. The pure culture without pre-enrichment 105 colony-forming units (CFU)/ml PA1, 103 CFU/ml 104 PA3 PA4. Then, standard curves established targets. showed perfect linear correlations, with R2 values 0.9901 PA12, 0.9915 0.9924 0.9935 PA15. real-time (qPCR) assay 102 cultures aeruginosa. Compared endpoint traditional methods, more sensitive by one or two orders magnitude. feasibility satisfactory terms sensitivity, specificity, efficiency after evaluating 29 ready-to-eat vegetable samples almost consistent that national method. developed assays be applied rapid screening pathogenic providing accurate results inform effective monitoring measures order improve microbiological safety.

Language: Английский

---

Protein film electrochemistry

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: Oct. 19, 2023

Language: Английский

---

Polyaniline Entrapped Water-Dispersible 3MPA-ZnSe Quantum Dots and Their Application for the Development of an Enzymatic Electrochemical Nanobiosensor for the Detection of 17β-Estradiol, an Endocrine-Disrupting Compound

Applied Biochemistry and Biotechnology, Journal Year: 2023, Volume and Issue: 195(5), P. 3425 - 3455

Published: Jan. 4, 2023

Language: Английский

---

Overview on the Development of Electrochemical Immunosensors by the Signal Amplification of Enzyme- or Nanozyme-Based Catalysis Plus Redox Cycling

Molecules, Journal Year: 2024, Volume and Issue: 29(12), P. 2796 - 2796

Published: June 12, 2024

Enzyme-linked electrochemical immunosensors have attracted considerable attention for the sensitive and selective detection of various targets in clinical diagnosis, food quality control, environmental analysis. In order to improve performances conventional immunoassays, significant efforts been made couple enzyme-linked or nanozyme-based catalysis redox cycling signal amplification. The current review summarizes recent advances development enzyme- with special features reactions their synergistic functions amplification are discussed. Additionally, challenges future directions addressed.

Language: Английский

---

Recent trends in enzyme engineering aiming to improve bioelectrocatalysis proceeding with direct electron transfer

Current Opinion in Electrochemistry, Journal Year: 2021, Volume and Issue: 31, P. 100856 - 100856

Published: Oct. 9, 2021

Language: Английский

---

Cytochrome P450 electrochemical biosensors transforming in vitro metabolism testing – Opportunities and challenges

Bioelectrochemistry, Journal Year: 2025, Volume and Issue: 163, P. 108913 - 108913

Published: Jan. 22, 2025

The ability of the living world to flourish in face constant exposure dangerous chemicals depends on management a widespread group enzymes known as heme-thiolate monooxygenases or cytochrome P450 superfamily. About three-quarters all reactions determining metabolism endogenous compounds, those carried foods, taken drugs, even synthetic discarded into environment depend their catalytic performance. chromatographic and (photo)luminometric methods routinely used predictive analytical tools laboratories have significant drawbacks ranging from limited shelf-life reagents, use substrates, laborious tedious procedures for highly sensitive detection. In this review, alternative electrochemical biosensors using bio-element are emphasized main aspects well regarding implementation usefulness. Despite various schemes proposed implementation, reports real applications scant several reasons, including low reaction rates, broad substrate specificity, uncoupling occurrence, need expensive electron transfer partners promote transfer. Finally, prospect future developments is introduced, focusing integrating miniaturized systems with techniques, alongside optimizing enzyme immobilization electrode modifications improve enzymatic stability enhance sensor reliability. This progress represents crucial step towards creation portable that mimic human physiological responses, supporting precision medicine approach.

Language: Английский

---

Miniaturized DyP Peroxidase-Based Biosensor for On-Site Open Air Use

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

A dye-decolorizing peroxidase (DyP)-based electrochemical biosensor for hydrogen peroxide (H2O2) is developed in miniaturized, disposable, and user-friendly configuration. Wild type variant DyPs are immobilized on self-assembled monolayer (SAM)-coated nanostructure-modified screen-printed electrodes (SPEs) to ensure biocompatibility increase the enzyme loading hence sensitivity. The structure of enzymes attached gold silver nanoparticle (AuNP AgNP)-modified carbon- gold-based SPEs (C-SPE Au-SPE) monitored by resonance Raman spectroscopy their electrocatalytic performance toward H2O2 electrochemistry. Among tested configurations, wild DyP/SAM/AuNP/C-SPE construct shows a superior performance, with linear response range from 30 475 μM, sensitivity 234 ± 9 mA·M-1·cm-2, limit detection (LOD) 3.5 measured open air conditions. device suitable single-use, on-site measurements air-exposed samples such as physiological fluids. herein high potential customization other small hydroperoxide substrates additional improvement selected traits (e.g., sensitivity, thermal stability, substrate inhibition properties) due easy well-established production genetic manipulation employed biocatalyst, DyP.

Language: Английский

---

Cytochromes P450 in biosensing and biosynthesis applications: Recent progress and future perspectives

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 158, P. 116791 - 116791

Published: Oct. 10, 2022

Language: Английский

---