Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering DOI Creative Commons

Hallie Thorp,

Kyung-Sook Kim,

Sophia Bou‐Ghannam

et al.

Regenerative Therapy, Journal Year: 2021, Volume and Issue: 18, P. 487 - 496

Published: Dec. 1, 2021

Advanced tissue engineering approaches for direct articular cartilage replacement in vivo employ mesenchymal stem cell (MSC) sources, exploiting innate chondrogenic potential to fabricate hyaline-like constructs vitro within three-dimensional (3D) culture conditions. Cell sheet technology represents one such advanced 3D scaffold-free platform, and previous work has shown that MSC sheets are capable of differentiation. The present study aims build upon this understanding elucidate the effects an established manipulation technique, multilayering, on fabrication MSC-derived layered vitro. To achieve goal, multilayered prepared assessed structural biochemical transitions throughout chondrogenesis. Results support multilayering as a means increasing construct thickness cellular interactions related chondrogenesis, including N-cadherin, connexin 43, integrin β-1. Data indicate from 14 μm (1-layer construct) 25 (2-layer increases these subsequent However, clear initial threshold (33 - 3-layer is evident decreases rate extent specifically gene expressions (Sox9, aggrecan, type II collagen) sulfated proteoglycan accumulation deposited extracellular matrix (ECM). Together, data utility platform tailoring chondrogenesis future regeneration applications.

Language: Английский

Recent advancements in cartilage tissue engineering innovation and translation DOI
Rachel C. Nordberg, Benjamin J. Bielajew, Takumi Takahashi

et al.

Nature Reviews Rheumatology, Journal Year: 2024, Volume and Issue: 20(6), P. 323 - 346

Published: May 13, 2024

Language: Английский

Citations

25
Main and Minor Types of Collagens in the Articular Cartilage: The Role of Collagens in Repair Tissue Evaluation in Chondral Defects DOI Open Access
Lourdes Alcaide‐Ruggiero, Verónica Molina-Hernández, María del Mar Granados Machuca

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(24), P. 13329 - 13329

Published: Dec. 11, 2021

Several collagen subtypes have been identified in hyaline articular cartilage. The main and most abundant collagens are type II, IX XI collagens. minor less III, IV, V, VI, X, XII, XIV, XVI, XXII, XXVII All these found to play a key role healthy cartilage, regardless of whether they more or abundant. Additionally, an exhaustive evaluation fibrils repaired cartilage tissue after chondral lesion is necessary determine the quality even not this considered Therefore, review aims describe depth all types normal structure, based on this, establish parameters that allow one consider as

Language: Английский

Citations

85
Functionalized Hydrogels for Articular Cartilage Tissue Engineering DOI Creative Commons
Liangbin Zhou, Peng Guo, Matteo D’Este

et al.

Engineering, Journal Year: 2022, Volume and Issue: 13, P. 71 - 90

Published: April 12, 2022

Articular cartilage (AC) is an avascular and flexible connective tissue located on the bone surface in diarthrodial joints. AC defects are common knees of young physically active individuals. Because lack suitable tissue-engineered artificial matrices, current therapies for defects, especially full-thickness osteochondral interfaces, fail to replace or regenerate damaged adequately. With rapid research development advancements engineering (ACTE), functionalized hydrogels have emerged as promising matrix substitutes because their favorable biomechanical properties, water content, swelling ability, cytocompatibility, biodegradability, lubricating behaviors. They can be rationally designed conveniently tuned simulate extracellular cartilage. This article briefly introduces composition, structure, function its followed by a comprehensive review exquisite (bio)design (bio)fabrication repair. Finally, we summarize challenges encountered hydrogel-based strategies ACTE both vivo vitro future directions clinical translation.

Language: Английский

Citations

52
Organ-on-a-chip: A new tool for in vitro research DOI
Jiasheng Yan, Ziwei Li, Jiuchuan Guo

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 216, P. 114626 - 114626

Published: Aug. 10, 2022

Language: Английский

Citations

43
Research progress of engineered mesenchymal stem cells and their derived exosomes and their application in autoimmune/inflammatory diseases DOI Creative Commons

Xueqing Zhu,

Dan Ma, Baoqi Yang

et al.

Stem Cell Research & Therapy, Journal Year: 2023, Volume and Issue: 14(1)

Published: April 11, 2023

Abstract Autoimmune/inflammatory diseases affect many people and are an important cause of global incidence mortality. Mesenchymal stem cells (MSCs) have low immunogenicity, immune regulation, multidifferentiation other biological characteristics, play role in tissue repair regulation widely used the research treatment autoimmune/inflammatory diseases. In addition, MSCs can secrete extracellular vesicles with lipid bilayer structures under resting or activated conditions, including exosomes, microparticles apoptotic bodies. Among them, as most component vesicles, function parent MSCs. Although their exosomes characteristics homing, engineering these through various technical means, such genetic engineering, surface modification further improve homing congenital make them specifically target specific tissues organs, therapeutic effect. This article reviews advanced technology MSC-derived its application some by searching literature published recent years at home abroad.

Language: Английский

Citations

25
Orthobiologics: a review DOI
Andreas F. Mavrogenis,

Vasileios Karampikas,

Alexandros Zikopoulos

et al.

International Orthopaedics, Journal Year: 2023, Volume and Issue: 47(7), P. 1645 - 1662

Published: April 18, 2023

Language: Английский

Citations

25
3D Printing for Bone-Cartilage Interface Regeneration DOI Creative Commons
Jialian Xu,

Jindou Ji,

Juyang Jiao

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Feb. 14, 2022

Due to the vasculature defects and/or avascular nature of cartilage, as well complex gradients for bone-cartilage interface regeneration and layered zonal architecture, self-repair cartilage subchondral bone is challenging. Currently, primary osteochondral defect treatment strategies, including artificial joint replacement autologous allogeneic graft, are limited by their ability simply repair, rather than induce tissues. Meanwhile, over past two decades, three-dimension (3D) printing technology has achieved admirable advancements in reconstruction, providing a new strategy restoring function. The advantages 3D hybrid materials include rapid accurate molding, personalized therapy. However, certain challenges also exist. For instance, reconstruction must simulate histological structure bone, thus, it necessary determine optimal bioink concentrations maintain mechanical strength cell viability, while identifying biomaterials with dual bioactivities capable simultaneously regenerating cartilage. study showed that crucial repair defect. In this review, we focus on significant progress application regeneration, expounding potential prospects highlighting some most currently facing field.

Language: Английский

Citations

30
Osteoarthritis models: From animals to tissue engineering DOI Creative Commons

Hongyuan Dou,

Shuhan Wang, Jiawei Hu

et al.

Journal of Tissue Engineering, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 1, 2023

Osteoarthritis (OA) is a chronic degenerative osteoarthropathy. Although it has been revealed that variety of factors can cause or aggravate the symptoms OA, pathogenic mechanisms OA remain unknown. Reliable models accurately reflect human disease are crucial for studies on mechanism and therapeutic drug evaluation. This review first demonstrated importance by briefly introducing pathological features current limitations in pathogenesis treatment OA. Then, mainly discusses development different models, including animal engineered highlighting their advantages disadvantages from perspective pathology analysis. In particular, state-of-the-art potential were emphasized, as they may represent future direction models. Finally, challenges obtaining reliable also discussed, possible directions outlined to shed some light this area.

Language: Английский

Citations

19
Biomimetic Approaches for the Design and Fabrication of Bone-to-Soft Tissue Interfaces DOI Creative Commons

Carlos Pitta Kruize,

Sara Panahkhahi,

N.E. Putra

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2021, Volume and Issue: 9(7), P. 3810 - 3831

Published: Nov. 16, 2021

Bone-to-soft tissue interfaces are responsible for transferring loads between tissues with significantly dissimilar material properties. The examples of connective soft ligaments, tendons, and cartilages. Such natural have unique microstructural properties characteristics which avoid the abrupt transitions two prevent formation stress concentration at their connections. Here, we review some important these interfaces. native bone-to-soft consist several hierarchical levels formed in a highly specialized anisotropic fashion composed different types heterogeneously distributed cells. interface can rely on main design principles, namely by changing local microarchitectural features (e.g., complex cell arrangements, introducing interlocking mechanisms through various geometrical designs) chemical compositions smooth gradual transition level mineralization). Implementing such principles appears to be promising approach that used design, reconstruction, regeneration engineered biomimetic Furthermore, prominent fabrication techniques as additive manufacturing (AM) including 3D printing electrospinning ease implementation processes. Biomimetic biological applications, example, create synthetic scaffolds osteochondral repair.

Language: Английский

Citations

41
Mesenchymal Stromal Cells for Aging Cartilage Regeneration: A Review DOI Open Access
Kun‐Chi Wu,

Yu-Hsun Chang,

Dah‐Ching Ding

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12911 - 12911

Published: Nov. 30, 2024

Cartilage degeneration is a key feature of aging and osteoarthritis, characterized by the progressive deterioration joint function, pain, limited mobility. Current treatments focus on symptom relief, not cartilage regeneration. Mesenchymal stromal cells (MSCs) offer promising therapeutic option due to their capability differentiate into chondrocytes, modulate inflammation, promote tissue This review explores potential MSCs for regeneration, examining biological properties, action mechanisms, applications in preclinical clinical settings. derived from bone marrow, adipose tissue, other sources can self-renew multiple cell types. In cartilage, they aid regeneration secreting growth factors cytokines that enhance repair immune responses. Recent studies show restore integrity, reduce improve although translation remains challenging limitations such as viability, scalability, regulatory concerns. Advancements MSC delivery, including scaffold-based approaches engineered exosomes, may effectiveness. Potential risks, tumorigenicity rejection, are also discussed, emphasizing need optimized treatment protocols large-scale trials develop effective, minimally invasive therapies

Language: Английский

Citations

5