Trans-anethole exerts protective effects on lipopolysaccharide-induced acute jejunal inflammation of broilers via repressing NF-κB signaling pathway DOI Creative Commons

Yichun Tong,

Caiyun Yu, Shun Chen

et al.

Poultry Science, Journal Year: 2022, Volume and Issue: 102(2), P. 102397 - 102397

Published: Dec. 9, 2022

This study aimed to explore the effects of trans-anethole (TA) on lipopolysaccharide (LPS)-induced acute jejunal inflammation model broilers. A total 160 one-day-old broilers (male; Arbor Acres) were randomly allocated into four treatment groups with 8 replicates 5 birds each. On d 20, dose mg/kg body weight LPS solution and equal amount sterile saline intraperitoneally injected LPS-challenged unchallenged broilers, respectively. Compared control group, decreased (P < 0.05) villus height (VH) ratio crypt depth (VCR) but increased (CD), meanwhile, enhanced 0.01) levels interleukin-6 (IL-6), interleukin-1beta (IL-1β) tumor necrosis factor-alpha (TNF-α) level interleukin-10 (IL-10). The group supplemented 600 TA had lower CD higher VCR than group. IL-10 IL-1β. mRNA expression IL-6, nuclear factor kappa B (NF-κB), TNF-α up-regulated inhibitor NF-κB alpha (IκBα) down-regulated by as compared genes caused LPS, well IκBα. Furthermore, protein IκBα p65, p65. conclusion this is that could exert protective effect LPS-induced via repressing activation optimal against

Language: Английский

Virus–Host Cell Interactions DOI Creative Commons
Thomas Hoenen, Allison Groseth

Cells, Journal Year: 2022, Volume and Issue: 11(5), P. 804 - 804

Published: Feb. 25, 2022

As obligate intracellular parasites, viruses are intimately interconnected with their host cells [...].

Language: Английский

Citations

23
Nanopore Direct RNA Sequencing Data Processing and Analysis Using MasterOfPores DOI
Luca Cozzuto, Anna Delgado-Tejedor, Antonio Hermoso

et al.

Methods in molecular biology, Journal Year: 2023, Volume and Issue: unknown, P. 185 - 205

Published: Jan. 1, 2023

Language: Английский

Citations

16
RNA m5C methylation modification: a potential therapeutic target for SARS-CoV-2-associated myocarditis DOI Creative Commons
Yan Xiong, Yanan Li, Weiwei Qian

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 23, 2024

The Corona Virus Disease (COVID-19), caused by the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2), has quickly spread worldwide and resulted in significant morbidity mortality. Although most infections are mild, some patients can also develop severe fatal myocarditis. In eukaryotic RNAs, 5-methylcytosine (m5C) is a common kind of post-transcriptional modification, which involved regulating various biological processes (such as RNA export, translation, stability maintenance). With rapid development m5C modification detection technology, studies related to viral ever-increasing. These have revealed that plays an important role stages replication, including transcription translation. According recent studies, methylation regulate SARS-CoV-2 infection modulating innate immune signaling pathways. However, specific SARS-CoV-2-induced myocarditis remains unclear. Therefore, this review aims provide insights into molecular mechanisms infection. Moreover, regulatory NSUN2 host response was highlighted. This may new directions for developing therapeutic strategies SARS-CoV-2-associated

Language: Английский

Citations

6
No evidence for epitranscriptomic m5C modification of SARS-CoV-2, HIV and MLV viral RNA DOI Open Access
Anming Huang, Lydia Riepler, Dietmar Rieder

et al.

RNA, Journal Year: 2023, Volume and Issue: 29(6), P. 756 - 763

Published: March 8, 2023

The addition of chemical groups to cellular RNA modulate fate and/or function is summarized under the term epitranscriptomic modification. More than 170 different modifications have been identified on RNA, such as tRNA, rRNA and, a lesser extent, other types. Recently, modification viral has received considerable attention possible additional mechanism regulating virus infection and replication. N 6-methyladenosine (m 6 A) C5-methylcytosine 5 C) most broadly studied in viruses. Various studies, however, reported varying results with regard number extent Here we investigated m C methylome SARS-CoV-2, reexamined sites HIV MLV. Using rigorous bisulfite-sequencing protocol stringent data analysis, found no evidence for presence these emphasize necessity optimizing experimental conditions bioinformatic analysis.

Language: Английский

Citations

10
An HIV-1 Reference Epitranscriptome DOI Creative Commons

Michael S. Bosmeny,

Adrian A. Pater,

Li Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 31, 2025

ABSTRACT Post-transcriptional chemical modifications to RNA, or the epitranscriptome, play important roles in RNA metabolism, gene regulation, and human disease, including viral pathogenesis. Modifications genome transcripts of immunodeficiency virus 1 (HIV-1) have been reported, methylation adenosine (m 6 A) cytosine 5 C), acetylation cytosine, pseudouridylation (psi), conversion inosine, their effects on host biology investigated. However, diverse experimental approaches used, making clear correlations across studies difficult assess. To address this need, we propose establishment a reference HIV-1 epitranscriptome. We sequenced model NL4-3 from infected Jurkat CD4+ T cells using latest nanopore chemistry, custom preparation methods, commercial base-calling algorithms. This resulted reproducible sense preliminary antisense epitranscriptome where m A, C, psi, ands inosine could be identified by multiplexed base-calling. Multiplexed miscalled due sequence neighboring modification contexts, which demonstrate can corrected with synthetic fragments. validate A sites small molecule inhibitor methyltransferase-like 3 (METTL3), STM2457. conclude that do not change substantially under combination antiretroviral therapy (cART) treatment primary cells. Samples patients living HIV reveal conservation certain modifications, such as A. Our approach data offer straightforward benchmark adopted help advance rigor, reproducibility, uniformity future epitranscriptomics studies.

Language: Английский

Citations

0
Resolving sequencing-based HIV-1 epitranscriptomics DOI

Michael S. Bosmeny,

João I. Mamede, Keith T. Gagnon

et al.

Epigenomics, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: May 16, 2025

The collection of HIV-1 RNA chemical modifications and their functional consequences in viral gene expression, host interactions, the life cycle, referred to as epitranscriptomics, remain incompletely understood. While field is evolving, diverse modification discovery methods, cell lines, sequences, bioinformatics methods make a consensus view epitranscriptome difficult resolve. Here, we review for identifying interpreting N6-methyladenosine (m6A), 5-methylcytosine (m5C), pseudouridine (Ψ), 2´-O-methylation (Nm), N4-acetylcytidine (ac4C) HIV-1, including antibody-based selection chemical-treatment-based detection by nanopore direct sequencing. We recommend adoption latter standardized sequencing strategy enable better benchmarking across studies help resolve epitranscriptomics.

Language: Английский

Citations

0
From RNA World to SARS-CoV-2: The Edited Story of RNA Viral Evolution DOI Creative Commons
Zachary W. Kockler, Dmitry A. Gordenin

Cells, Journal Year: 2021, Volume and Issue: 10(6), P. 1557 - 1557

Published: June 20, 2021

The current SARS-CoV-2 pandemic underscores the importance of understanding evolution RNA genomes. While is subject to formation similar lesions as DNA, evolutionary and physiological impacts have on viral genomes are yet be characterized. Lesions that may drive can induce breaks repaired by recombination or cause base substitution mutagenesis, also known editing. Over past decade so, editing mutagenesis DNA has been many studies, revealing exposure ssDNA hypermutation involved in etiology cancer. However, not studied same extent. Recently single-stranded documented though its role population dynamics. Here, we will summarize knowledge key mechanisms causes genome instability covering areas from world theory today. We highlight questions remain it pertains instability, mutations accumulation, experimental strategies for addressing these questions.

Language: Английский

Citations

23
Interplay of RNA 2′-O-methylations with viral replication DOI Creative Commons
Alice Decombe, Priscila El Kazzi, Étienne Decroly

et al.

Current Opinion in Virology, Journal Year: 2023, Volume and Issue: 59, P. 101302 - 101302

Published: Feb. 8, 2023

Language: Английский

Citations

9
Grapevine Virology in the Third-Generation Sequencing Era: From Virus Detection to Viral Epitranscriptomics DOI Creative Commons
Vahid Jalali Javaran, Peter Moffett,

Pierre Lemoyne

et al.

Plants, Journal Year: 2021, Volume and Issue: 10(11), P. 2355 - 2355

Published: Oct. 31, 2021

Among all economically important plant species in the world, grapevine (Vitis vinifera L.) is most cultivated fruit plant. It has a significant impact on economies of many countries through wine and fresh dried production. In recent years, grape industry been facing outbreaks known emerging viral diseases across world. Although high-throughput sequencing (HTS) used extensively virology, application potential third-generation have not explored understanding viruses their grapevine. Nanopore sequencing, technology, can be for direct both RNA DNA with minimal infrastructure. Compared to other HTS methods, MinION nanopore platform faster more cost-effective allows long-read sequencing. Due size device, it easily carried field disease surveillance. This review article discusses viruses, principle platforms, technology virus detection, virus–plant interactions, as well characterization modifications.

Language: Английский

Citations

20
RNA Modifications in Pathogenic Viruses: Existence, Mechanism, and Impacts DOI Creative Commons
Yongjin Zou,

Zijun Guo,

Xing‐Yi Ge

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2373 - 2373

Published: Nov. 20, 2024

RNA modification is a key posttranscriptional process playing various biological roles, and one which has been reported to exist extensively in cellular RNAs. Interestingly, recent studies have shown that viral RNAs also contain variety of modifications, are regulated dynamically by host machinery play critical roles different stages the life cycle. In this review, we summarize reports four typical modifications on RNAs, including N6-methyladenosine (m6A), 5-methylcytosine (m5C), N4-acetylcytosine (ac4C), N1-methyladenosine (m1A), describe molecular mechanisms these processes, illustrate their impacts replication, pathogenicity, innate immune responses. Notably, find viruses share some common features generation, regulation, function, highlighting potential for related serve as targets or bases development antiviral therapeutics vaccines.

Language: Английский

Citations

2