The Metabolomic Footprint of Liver Fibrosis DOI Creative Commons
Diren Beyoğlu, Yury Popov, Jeffrey R. Idle

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(16), P. 1333 - 1333

Published: Aug. 11, 2024

Both experimental and clinical liver fibrosis leave a metabolic footprint that can be uncovered defined using metabolomic approaches. Metabolomics combines pattern recognition algorithms with analytical chemistry, in particular, 1H 13C nuclear magnetic resonance spectroscopy (NMR), gas chromatography–mass spectrometry (GC–MS) various liquid (LC–MS) platforms. The analysis of by each these methodologies is reviewed separately. Surprisingly, there was little general agreement between studies within three groups also groups. determined NMR (two or more hits studies) comprised elevated lactate, acetate, choline, 3-hydroxybutyrate, glucose, histidine, methionine, glutamine, phenylalanine, tyrosine citrate. For GC–MS, succinate, fumarate, malate, ascorbate, glutamate, glycine, serine and, NMR, citrate were delineated. LC–MS, only β-muricholic acid, tryptophan, acylcarnitine, p-cresol, valine phosphocholine identified. upregulated as regards tyrosine, phosphocholine. Several investigators employed traditional Chinese medicine (TCM) treatments to reverse fibrosis, commentary given on the chemical constituents may possess fibrolytic activity. It proposed molecular docking procedures TCM lead novel therapies for affecting at least one-in-twenty persons globally, which currently no pharmaceutical cure. This in-depth review summarizes relevant literature metabolomics its implications addressing problem cirrhosis sequelae.

Language: Английский

The Role of Bovine Milk-Derived Exosomes in Human Health and Disease DOI Creative Commons
Monika Jabłońska, Tomasz Sawicki, Justyna Żulewska

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(24), P. 5835 - 5835

Published: Dec. 11, 2024

Bovine milk is widely recognized as one of the most valuable sources nutrients such proteins, fats, vitamins, and minerals that support development health body. In recent years, there has been increasing scientific interest in exosomes, small membrane-bound vesicles found milk. Through their content (e.g., microRNA), exosomes can influence gene expression modulate key signaling pathways within target cells. Results from vitro vivo studies have shown bovine milk-derived alleviate intestinal inflammation by regulating positively influencing composition gut microbiota. They also improve cognitive function nervous system regeneration. addition, promote bone stimulating osteoblast formation inhibiting resorption, helping to prevent osteoporosis. Studies beneficial effects on skin promoting collagen production, protecting cells oxidative stress, delaying ageing process. are a promising tool for treatment prevention variety diseases, particularly those related tissue Although these results promising, further needed fully understand mechanisms action potential clinical application different diseases.

Language: Английский

Citations

3
Significance of CCNs in liver regeneration DOI

Joshua Max Barkin,

Brady Jin-Smith,

Kendle Torok

et al.

Journal of Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 17(2), P. 321 - 332

Published: May 18, 2023

Language: Английский

Citations

9
Hypoxia enhances anti-fibrotic properties of extracellular vesicles derived from hiPSCs via the miR302b-3p/TGFβ/SMAD2 axis DOI Creative Commons
Milena Paw, Agnieszka A. Kusiak, Kinga Nit

et al.

BMC Medicine, Journal Year: 2023, Volume and Issue: 21(1)

Published: Oct. 31, 2023

Cardiac fibrosis is one of the top killers among fibrotic diseases and continues to be a global unaddressed health problem. The lack effective treatment combined with considerable socioeconomic burden highlights urgent need for innovative therapeutic options. Here, we evaluated anti-fibrotic properties extracellular vesicles (EVs) derived from human induced pluripotent stem cells (hiPSCs) that were cultured under various oxygen concentrations.EVs isolated three hiPSC lines normoxia (21% O2; EV-N) or reduced concentration (hypoxia): 3% O2 (EV-H3) 5% (EV-H5). activity EVs was tested in an vitro model cardiac fibrosis, followed by detailed investigation underlying molecular mechanisms. Sequencing EV miRNAs bioinformatics analysis conducted selected miRNA validated using mimic inhibitor. Finally, mouse angiotensin II-induced fibrosis.We provide evidence enhances effects hiPS-EVs. These more reducing pro-fibrotic markers activated fibroblasts, when compared EV-N EV-H3. We show EV-H5 act through canonical TGFβ/SMAD pathway, primarily via miR-302b-3p, which most abundant EV-H5. Our results not only target transcripts several profibrotic genes, including SMAD2 TGFBR2, but also reduce stiffness fibroblasts. In heart outperformed suppressing inflammatory response host attenuating collagen deposition tissue.In this work, superior over study uncovers fine regulation cellular environment may enhance hiPS-EVs, has great potential applied regeneration.

Language: Английский

Citations

8
TGF-β1 induces formation of TSG-6-enriched extracellular vesicles in fibroblasts which can prevent myofibroblast transformation by modulating Erk1/2 phosphorylation DOI Creative Commons
Marcus M. Ilg, Stephen A. Bustin,

David Ralph

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 29, 2024

Extracellular vesicles have emerged as important mediators of cell-to-cell communication in the pathophysiology fibrotic diseases. One such disease is Peyronie's (PD), a disorder penis caused by uncontrolled transformation resident fibroblasts to alpha-smooth muscle actin positive myofibroblasts. These cells produce large amounts extracellular matrix, leading formation plaque penile tunica albuginea (TA), causing pain, curvature, and erectile dysfunction. We used primary derived from TA PD patients explore role transforming growth factor beta 1 (TGF-β1), key signalling this process. TGF-β1 treatment elicited range responses myofibroblasts: (i) they secreted (EVs) that were more numerous differed size shape those fibroblasts, (ii) these EVs prevented TGF-β1-induced manner was dependent on vesicle uptake (iii) phosphorylation Erk1/2, critical component modulating fibrogenic phenotypic responses, but did not affect Smad-signalling. posit effect could be linked enrichment TSG-6 myofibroblast-derived EVs. The ability prevent further myofibroblast may establish them part an anti-fibrotic negative feedback loop, with potential exploited for future therapeutic approaches.

Language: Английский

Citations

2
The Metabolomic Footprint of Liver Fibrosis DOI Creative Commons
Diren Beyoğlu, Yury Popov, Jeffrey R. Idle

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(16), P. 1333 - 1333

Published: Aug. 11, 2024

Both experimental and clinical liver fibrosis leave a metabolic footprint that can be uncovered defined using metabolomic approaches. Metabolomics combines pattern recognition algorithms with analytical chemistry, in particular, 1H 13C nuclear magnetic resonance spectroscopy (NMR), gas chromatography–mass spectrometry (GC–MS) various liquid (LC–MS) platforms. The analysis of by each these methodologies is reviewed separately. Surprisingly, there was little general agreement between studies within three groups also groups. determined NMR (two or more hits studies) comprised elevated lactate, acetate, choline, 3-hydroxybutyrate, glucose, histidine, methionine, glutamine, phenylalanine, tyrosine citrate. For GC–MS, succinate, fumarate, malate, ascorbate, glutamate, glycine, serine and, NMR, citrate were delineated. LC–MS, only β-muricholic acid, tryptophan, acylcarnitine, p-cresol, valine phosphocholine identified. upregulated as regards tyrosine, phosphocholine. Several investigators employed traditional Chinese medicine (TCM) treatments to reverse fibrosis, commentary given on the chemical constituents may possess fibrolytic activity. It proposed molecular docking procedures TCM lead novel therapies for affecting at least one-in-twenty persons globally, which currently no pharmaceutical cure. This in-depth review summarizes relevant literature metabolomics its implications addressing problem cirrhosis sequelae.

Language: Английский

Citations

2