Dynamic Stimulations with Bioengineered Extracellular Matrix‐Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 10(21)

Published: April 29, 2023

Abstract Cells interact with their surrounding environment through a combination of static and dynamic mechanical signals that vary over stimulus types, intensity, space, time. Compared to such as stiffness, porosity, topography, the current understanding on effects stimulations cells remains limited, attributing lack access devices, complexity experimental set‐up, data interpretation. Yet, in pursuit emerging translational applications (e.g., cell manufacturing for clinical treatment), it is crucial understand how respond variety forces are omnipresent vivo so they can be exploited enhance therapeutic outcomes. With rising appreciation extracellular matrix (ECM) key regulator biofunctions, researchers have bioengineered suite ECM‐mimicking hydrogels, which fine‐tuned spatiotemporal cues model complex profiles. This review first discusses stimuli may impact different cellular components various mechanobiology pathways involved. Then, hydrogels designed incorporate parameters influence behaviors described. The Scopus database also used analyze relative strength evidence, ranging from strong weak, based number published literatures, associated citations, treatment significance. Additionally, impacts clinically relevant types including mesenchymal stem cells, fibroblasts, immune evaluated. aim draw attention paucity studies well highlight potential using cocktail intensities fates (similar concept biochemical direct fate). It envisioned this progress report will inspire more exciting development mechanoresponsive biomedical applications.

Language: Английский

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Hydrogel based tissue engineering and its future applications in personalized disease modeling and regenerative therapy

Beni-Suef University Journal of Basic and Applied Sciences, Journal Year: 2022, Volume and Issue: 11(1)

Published: Jan. 4, 2022

Abstract Background Evolution in the vitro cell culture from conventional 2D to 3D technique has been a significant accomplishment. The models have provided close and better insight into physiological study of human body. increasing demand for organs like liver, kidney, pancreas transplantation, rapid anti-cancer drug screening, limitations associated with use animal attracted interest researchers explore organ culture. Main body Natural, synthetic, hybrid material-based hydrogels are being used as scaffolds provide 'close-to-in vivo’ structures. Organoids: stem cell-derived small size systems now favored due their ability mimic in-vivo conditions or tissue this characteristic made it eligible variety clinical applications, discovery regenerative medicine few many areas application. applications long-time ethical biological challenge get accurate outcomes. bioprinting resolved issue vascularization organoid great extent by its layer-by-layer construction approach. bioprinted organoids popular application personalized disease modeling development therapeutics. Short conclusions This review paper, focuses on discussing novel approach, advantages limitations, potential life science namely cancer research, therapy, engineering, discovery. Graphical

Language: Английский

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Transferrin-modified chitosan nanoparticles for targeted nose-to-brain delivery of proteins

Drug Delivery and Translational Research, Journal Year: 2022, Volume and Issue: 13(3), P. 822 - 838

Published: Oct. 7, 2022

Nose-to-brain delivery presents a promising alternative route compared to classical blood-brain barrier passage, especially for the of high molecular weight drugs. In general, macromolecules are rapidly degraded in physiological environment. Therefore, nanoparticulate systems can be used protect biomolecules from premature degradation. Furthermore, targeting ligands on surface nanoparticles able improve bioavailability by enhancing cellular uptake due specific binding and longer residence time. this work, transferrin-decorated chitosan evaluate passage model protein through nasal epithelial vitro. It was demonstrated that strain-promoted azide-alkyne cycloaddition reaction utilized attach functional group both transferrin enabling rapid covalent surface-conjugation under mild conditions after nanoparticle preparation. The intactness its efficiency were confirmed via SDS-PAGE SPR measurements. Resulting exhibited size about 110-150 nm with positive potential. Nanoparticles highest amount bound ligand also displayed into human cell line (RPMI 2650). an air-liquid interface co-culture glioblastoma cells (U87), showed faster layer as well increased cells. These findings demonstrate beneficial characteristics ligand. With chemical technological formulation concept, variety attached formation while maintaining cargo integrity.

Language: Английский

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Impact of lymphadenectomy extent on immunotherapy efficacy in postresectional recurred non-small cell lung cancer: a multi-institutional retrospective cohort study

International Journal of Surgery, Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 27, 2023

Lymph node (LN) dissection is a common procedure for non-small cell lung cancer (NSCLC) to ascertain disease severity and treatment options. However, murine studies have indicated that excising tumor-draining LNs diminished immunotherapy effectiveness, though its applicability clinical patients remains uncertain. Hence, the authors aim illustrate immunological implications of LN by analyzing impact dissected (DLN) count on efficacy, propose novel 'immunotherapy-driven' strategy.

Language: Английский

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Immunotoxicity of nanomaterials in health and disease: Current challenges and emerging approaches for identifying immune modifiers in susceptible populations

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2022, Volume and Issue: 14(6)

Published: Nov. 1, 2022

Abstract Nanosafety assessment has experienced an intense era of research during the past decades driven by a vivid interest regulators, industry, and society. Toxicological assays based on in vitro cellular models have undergone evolution from experimentation using nanoparticulate systems singular epithelial cell to employing advanced complex more realistically mimicking respective body barriers for analyzing their capacity alter immune state exposed individuals. During this phase, number lessons were learned. We thus arrived at where next chapters be opened, pursuing following objectives: (1) elucidate underlying mechanisms, (2) address effects vulnerable groups, (3) test material mixtures, (4) use realistic doses (5) sophisticated models. Moreover, data reproducibility become significant demand. In context, we studied emerging concept adverse outcome pathways (AOPs) perspective activation modulation resulting pro‐inflammatory versus tolerogenic responses. When considering interaction nanomaterials with biological systems, protein corona formation represents relevant molecular initiating event (e.g., potential alterations nanomaterial‐adsorbed proteins). Using as example, illustrate how integrated experimental–computational workflows combining silico aid enrichment upon comprehensive ontology‐annotated (meta)data upload online repositories assure FAIRness (Findability, Accessibility, Interoperability, Reusability). Such digital twinning may, future, assist early‐stage decision‐making therapeutic development, hence, promote safe‐by‐design innovation nanomedicine. it combination silico‐based exposure‐relevant dose‐finding, serve risk monitoring particularly loaded areas, workplaces, taking into account pre‐existing health conditions. This article is categorized under: Toxicology Regulatory Issues Nanomedicine > Nanomaterials

Language: Английский

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Three-Dimensional (3D) in vitro cell culture protocols to enhance glioblastoma research

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(2), P. e0276248 - e0276248

Published: Feb. 8, 2023

Three-dimensional (3D) cell culture models can help bridge the gap between in vitro cultures and vivo responses by more accurately simulating natural environment, shape, tissue stiffness, stressors, gradients cellular response while avoiding costs ethical concerns associated with animal models. The inclusion of third dimension 3D influences spatial organization surface receptors that interact other cells imposes physical restrictions on compared to Two-dimensional (2D) cultures. Spheroids’ distinctive cyto-architecture mimics structure, gene expression, metabolism, proliferation, oxygenation, nutrition absorption, waste excretion, drug uptake preserving cell–extracellular matrix (ECM) connections communication, hence influencing molecular processes phenotypes. This protocol describes generation tumourspheroids using low attachment plate, hanging drop cellusponge scaffold based methods. expected results from these protocols confirmed ability all methods create uniform tumourspheres.

Language: Английский

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Corneal fibrosis: From in vitro models to current and upcoming drug and gene medicines

Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 209, P. 115317 - 115317

Published: April 19, 2024

Fibrotic diseases are characterised by myofibroblast differentiation, uncontrolled pathological extracellular matrix accumulation, tissue contraction, scar formation and, ultimately / organ dysfunction. The cornea, the transparent located on anterior chamber of eye, is extremely susceptible to fibrotic diseases, which cause loss corneal transparency and often associated with blindness. Although topical corticosteroids antimetabolites extensively used in management fibrosis, they glaucoma, cataract formation, corneoscleral melting infection, imposing need far more effective therapies. Herein, we summarise discuss shortfalls recent advances vitro models (e.g. transforming growth factor-β (TGF-β) ascorbic acid interleukin (IL) induced) drug TGF-β inhibitors, epigenetic modulators) gene editing, silencing) therapeutic strategies fibrosis context. Emerging therapeutical agents neutralising antibodies, ligand traps, receptor kinase antisense oligonucleotides) that have shown promise clinical setting but not yet assessed context also discussed.

Language: Английский

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Recent advances in Organ-on-a-Chip models: How precision engineering integrates cutting edge technologies in fabrication and characterization

Applied Materials Today, Journal Year: 2024, Volume and Issue: 38, P. 102231 - 102231

Published: May 14, 2024

Language: Английский

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Tumor Spheroids as Model to Design Acoustically Mediated Drug Therapies: A Review

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(3), P. 806 - 806

Published: March 1, 2023

Tumor spheroids as well multicellular tumor (MCTSs) are promising 3D in vitro models for drug screening, design, targeting, toxicity, and validation of delivery methods. These partly reflect the tridimensional architecture tumors, their heterogeneity microenvironment, which can alter intratumoral biodistribution, pharmacokinetics, pharmacodynamics drugs. The present review first focuses on current spheroid formation methods then investigations exploiting MCTS designing validating acoustically mediated therapies. We discuss limitations studies future perspectives. Various enable easy reproducible generation MCTSs. development assessment therapies have been mainly demonstrated made up cells only. Despite results obtained with these spheroids, successful evaluation will need to be addressed more relevant vascular using MCTS-on-chip platforms. MTCSs generated from patient-derived cancer nontumor cells, such fibroblasts, adipocytes, immune cells.

Language: Английский

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Advances in 3D culture systems for therapeutic discovery and development in brain cancer

Drug Discovery Today, Journal Year: 2022, Volume and Issue: 28(2), P. 103426 - 103426

Published: Nov. 1, 2022

Language: Английский

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