Unwinding circular RNA’s role in inflammatory pulmonary diseases

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2023, Volume and Issue: 397(5), P. 2567 - 2588

Published: Nov. 2, 2023

Language: Английский

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An Effective Peptide-Based Platform for Efficient Exosomal Loading and Cellular Delivery of a microRNA

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(3), P. 3851 - 3866

Published: Jan. 13, 2023

Exosomes, membrane-bound nanosized vesicles of biologic origin, are known to contain various molecules, e.g., proteins, lipids, and nucleic acids, which contribute the exosomes' ability mediate cell-to-cell communication. Recent impediments artificial nanoparticles in drug delivery, including low cellular uptake, activation immune system, tissue obstacles, have led scientists engineer exosomes as delivery vehicles. Though possess inherent properties stability, biocompatibility, immunogenicity, capability cross biological barriers, there is a need develop technologies that allow efficient loading therapeutic materials into exosomes. Here, we introduced simple peptide-equipped technology can enhance cargo-loading potential mild environment. Specifically, cell-penetrating peptide, YARA, derived from human immunodeficiency virus-1 trans-activator transcription, was covalently conjugated with miR-21-5p, mammalian microRNA. The conjugate YARA-miR-21-5p then incubated exosomes, isolated either mesenchymal stem cells or cancer cells, for loading. Exosomal time-dependent demonstrated an impressive 18.6-fold increase efficiency over exosomal miR-21-5p through incubation. After effective loaded rapidly delivered cells. Relative unloaded free YARA-miR-21-5p, significantly enhanced proliferation, migration, invasion mouse fibroblasts, vital steps wound healing. This study lays groundwork using peptides innovative approach efficiently load cargos, microRNAs, be employed deliver cargos yield effects.

Language: Английский

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Exosomes: A Promising Strategy for Repair, Regeneration and Treatment of Skin Disorders

Cells, Journal Year: 2023, Volume and Issue: 12(12), P. 1625 - 1625

Published: June 14, 2023

The skin is the organ that serves as outermost layer of protection against injury, pathogens, and homeostasis with external factors; in turn, it can be damaged by factors such burns, trauma, exposure to ultraviolet light (UV), infrared radiation (IR), activating signaling pathways Toll-like receptors (TLR) Nuclear factor erythroid 2-related 2 (NRF2), among others, causing a need subsequently repair regenerate skin. However, pathologies diabetes lengthen inflammatory stage, complicating healing process and, some cases, completely inhibiting it, generating susceptibility infections. Exosomes are nano-sized extracellular vesicles isolated purified from different sources blood, urine, breast milk, saliva, umbilical cord bile cells, mesenchymal stem cells. They have bioactive compounds that, thanks their paracrine activity, proven effective anti-inflammatory agents, inducers macrophage polarization accelerators regeneration, reducing possible complications relating poor wound repair, prolonged inflammation. This review provides information on use exosomes promising therapy damage UV light, radiation, disorders.

Language: Английский

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Engineering strategies and optimized delivery of exosomes for theranostic application in nerve tissue

Theranostics, Journal Year: 2023, Volume and Issue: 13(12), P. 4266 - 4286

Published: Jan. 1, 2023

Severe injuries or diseases affecting the peripheral and central nervous systems can result in impaired organ function permanent paralysis.Conventional interventions, such as drug administration cell-based therapy, exhibit limited effectiveness due to their inability preserve post-implantation cell survival impede deterioration of adjacent tissues.Exosomes have recently emerged powerful tools for tissue repair owing proteins nucleic acids, well unique phospholipid properties, which facilitate targeted delivery recipient cells.Engineering exosomes, obtained by manipulating parental cells directly functionalizing play critical roles enhancing regenerative repair, reducing inflammation, maintaining physiological homeostasis.Furthermore, exosomes been shown restore neurological when used combination with biomaterials.This paper primarily focuses on engineering strategies routes related neural research emphasizes theranostic application optimized nerve, traumatic spinal cord, brain injuries.Finally, prospects development other approaches will be discussed enhance our knowledge diseases.

Language: Английский

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Advances in the study of exosomes derived from mesenchymal stem cells and cardiac cells for the treatment of myocardial infarction

Cell Communication and Signaling, Journal Year: 2023, Volume and Issue: 21(1)

Published: Aug. 14, 2023

Acute myocardial infarction has long been the leading cause of death in coronary heart disease, which is characterized by irreversible cardiomyocyte and restricted blood supply. Conventional reperfusion therapy can further aggravate injury. Stem cell therapy, especially with mesenchymal stem cells (MSCs), emerged as a promising approach to promote cardiac repair improve function. MSCs may induce these effects secreting exosomes containing therapeutically active RNA, proteins lipids. Notably, normal function depends on intracardiac paracrine signaling via exosomes, secreted partially reflect changes during so analyzing vesicles provide valuable insights into pathology well guide development new treatments. The present review examines how produced influence injury after serve therapies against such Video Abstract.

Language: Английский

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