The relevance of sperm morphology in male infertility DOI Creative Commons
Elena Moretti, Cinzia Signorini, Daria Noto

et al.

Frontiers in Reproductive Health, Journal Year: 2022, Volume and Issue: 4

Published: Aug. 3, 2022

This brief report concerns the role of human sperm morphology assessment in different fields male infertility: basic research, genetics, assisted reproduction technologies, oxidative stress. One best methods studying is transmission electron microscopy (TEM) that enables defining concept pathology and classifying alterations non-systematic systematic. Non-systematic defects affect head tail variable ratio, whereas rare systematic are characterized by a particular anomaly marks most an ejaculate. TEM analysis fluorescence situ hybridization represent outstanding study cytogenetic patients with altered karyotype characterizing their semen quality before intracytoplasmic injection. In recent years, genetic investigations on defects, made extraordinary progress identifying candidate genes whose mutations induce morphological anomalies. The question if has impact fertilization outcome debated. Nowadays, stress represents one important causes function can be analyzed from two points view: 1) spermatozoa cytoplasmic residue produce reactive oxygen species, 2) pathologies inflammatory/oxidative background cause alterations. Finally, also considered endpoint vitro experiments where toxic substances, drugs, antioxidants tested. We think field far being exhausted needs other research. parameter still valuable indicator dysfunction both clinical

Language: Английский

Computer software (SiD) assisted real-time single sperm selection associated with fertilization and blastocyst formation DOI Creative Commons
Gerardo Mendizabal‐Ruiz, Alejandro Chávez-Badiola,

Isaac Aguilar Figueroa

et al.

Reproductive BioMedicine Online, Journal Year: 2022, Volume and Issue: 45(4), P. 703 - 711

Published: April 10, 2022

Language: Английский

Citations

35
Improving outcomes of assisted reproductive technologies using artificial intelligence for sperm selection DOI Creative Commons

Nicole Lustgarten Guahmich,

Elena Borini,

Nikica Zaninović

et al.

Fertility and Sterility, Journal Year: 2023, Volume and Issue: 120(4), P. 729 - 734

Published: June 10, 2023

Language: Английский

Citations

17
Sperm Preparation with Microfluidic Sperm Sorting Chip May Improve Intracytoplasmic Sperm Injection Outcomes Compared to Density Gradient Centrifugation DOI Creative Commons

Maria Banti,

Estee Van Zyl, Dimitrios Kafetzis

et al.

Reproductive Sciences, Journal Year: 2024, Volume and Issue: 31(6), P. 1695 - 1704

Published: Feb. 23, 2024

Abstract Does sperm preparation using the FERTILE PLUS™ Sperm Sorting Chip improve fertilization rates, blastocyst formation, utilization, and euploidy rates in patients undergoing intracytoplasmic injection (ICSI), compared with density gradient centrifugation (DGC)? A single-cohort, retrospective data review including from 53 couples who underwent ICSI cycles within a 12-month period. For each couple, two closest, consecutive were identified, where one used standard technique of (DGC) subsequent PLUS™, therefore, acted as their own controls. Paired samples t -test was to compare means for outcomes (fertilization, rates). Binary logistic regression analysis assessed relationship between female age, presence male factor infertility, rates. Blastocyst, significantly higher DGC (76% vs 56%, p = 0.002; 60% 41%, 0.005, 40% 20%, 0.001, respectively). Although there an increase this not significant (72% 68%, 0.449). The females ≤ 35 years increased when method used, older age group (OR 2.31, 0.007). No association found or absence infertility cycles. This study provides tentative evidence that microfluidic sorting device selection can following comparison method.

Language: Английский

Citations

6
Advanced Paternal Age and Future Generations DOI Creative Commons
Peter Chan, Bernard Robaire

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: June 9, 2022

Paternal age at conception has been increasing. In this review, we first present the results from major mammalian animal models used to establish that increasing paternal does affect progeny outcome. These provide several advantages including possibility assess multi- transgenerational effects of on in a relatively short time window. We then clinical observations relating advanced fertility and offspring with respect perinatal health, cancer risk, genetic diseases, neurodevelopmental effects. An overview potential mechanism operating altering germ cells is presented. This followed by an analysis current state management reproductive risks associated age. The numerous challenges developing effective, practical strategies mitigate impact are outlined along approach how move forward important quandary.

Language: Английский

Citations

24
Microfluidic sperm sorting selects a subpopulation of high-quality sperm with a higher potential for fertilization DOI
Nadia Sheibak,

Fatemehsadat Amjadi,

Amir Shamloo

et al.

Human Reproduction, Journal Year: 2024, Volume and Issue: 39(5), P. 902 - 911

Published: March 10, 2024

Abstract STUDY QUESTION Is a microfluidic sperm sorter (MSS) able to select higher quality compared conventional methods? SUMMARY ANSWER The MSS selects with improved parameters, lower DNA fragmentation, and fertilizing potential. WHAT IS KNOWN ALREADY To date, the few studies that have microfluidics selection methods used heterogeneous study population lacked molecular investigations. DESIGN, SIZE, DURATION efficiency of newly designed in isolating high-quality was density-gradient centrifugation (DGC) swim-up (SU) methods, using 100 semen samples two groups, during 2023–2024. PARTICIPANTS/MATERIALS, SETTING, METHODS Semen specimens from 50 normozoospermic non-normozoospermic men were sorted MSS, DGC, SU compare parameters related potential sperm. determined by measurement phospholipase C zeta (PLCζ) post-acrosomal sheath WW domain-binding protein (PAWP) expression flow cytometry, chromatin dispersion test assess damage. MAIN RESULTS AND THE ROLE OF CHANCE In both MSS-selected highest progressive motility, PLCζ positive PAWP fluorescence intensity lowest non-progressive minimal selected DGC (P < 0.05). LIMITATION, REASONS FOR CAUTION major limitations our low yield chips intentional exclusion severe male factor infertility sufficient count for experiments; thus testing oligozoospermic motility is still required. addition, due ethical considerations, at present, it impossible use achieved clinic fertilization rate further outcomes. WIDER IMPLICATIONS FINDINGS Our research presents new evidence sorting may result raw semen. This novel technology might be key improving clinical outcomes assisted reproduction infertile patients. FUNDING/COMPETING INTEREST(S) funded Iran University Medical Sciences no competing interest exists. TRIAL REGISTRATION NUMBER N/A.

Language: Английский

Citations

5
New horizons in human sperm selection for assisted reproduction DOI Creative Commons
Brett Nixon, John E. Schjenken, Nathan D. Burke

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 22, 2023

Male infertility is a commonly encountered pathology that estimated to be contributory factor in approximately 50% of couples seeking recourse assisted reproductive technologies. Upon clinical presentation, such males are subjected conventional diagnostic andrological practices rely on descriptive criteria define their fertility based the number morphologically normal, motile spermatozoa within ejaculate. Despite virtual ubiquitous adoption practices, they not without limitations and accordingly, there now increasing awareness importance assessing sperm quality order more accurately predict male's status. This realization raises important question which characteristics signify high-quality, fertilization competent cell. In this review, we reflect recent advances our mechanistic understanding biology function, contributing growing armory innovative approaches diagnose treat male infertility. particular review progress toward implementation precision medicine; robust setting fertility, currently lags well behind other fields medicine. this, research shows application advanced technology platforms as whole exome sequencing proteomic analyses hold considerable promise optimizing outcomes for management by uncovering expanding inventory candidate biomarkers, those associated with recurrent pregnancy loss. Similarly, development imaging technologies tandem machine learning artificial intelligence poised disrupt care paradigm advancing molecular biological causes provide novel avenues future diagnostics treatments.

Language: Английский

Citations

13
The Polymorphism Asn680Ser on the FSH Receptor and Abnormal Ovarian Response in Patients with Normal Values of AMH and AFC DOI Open Access
Giorgio Maria Baldini, Assunta Catino, Simone Palini

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 1080 - 1080

Published: Jan. 5, 2023

After the controlled ovarian stimulation (COS), number of cumulus oocyte complexes collected is lower than predicted. The aim this study to understand if there a possible reason for that deficient response. It was hypothesized associated with SNP (single-nucleotide polymorphism) FSH receptor (FSHr), specifically c.2039A > G, resulting in Asn680Ser. Two groups patients were enrolled purpose: normal (n = 36) and abnormal responses 31). To predict retrievable oocytes, according anti-Mũllerian hormone (AMH) antral follicle count (AFC), following formula applied log scale: oocytes retrieved 2.584 − 0.015 × (age) 0.035 (FSH) + 0.038 0.026 (AFC). Then, when less 50% calculated value, it proposed result an DNA sample blood from women, then genetic assessment Asn680Ser FSHr evaluated both groups. differences between two categories statistically analyzed independent samples t test, Mann−Whitney U test Chi-squared test. In patient response, significant prevalence amino acid serine at position 680 compared counterpart group (p < 0.05) detected. conclusion, results, evaluation could represent accurate predictive feature undergoing assisted reproductive technology treatment.

Language: Английский

Citations

11
The impact of microfluidics sperm processing on blastocyst euploidy rates compared with density gradient centrifugation: a sibling oocyte double-blinded prospective randomized clinical trial DOI
Prachi N. Godiwala,

Jane Kwieraga,

Emilse Almanza

et al.

Fertility and Sterility, Journal Year: 2024, Volume and Issue: 122(1), P. 85 - 94

Published: Feb. 15, 2024

Language: Английский

Citations

4
Advanced Sperm Selection Techniques for Assisted Reproduction DOI Open Access
Federica Cariati, Marina Orsi,

Francesca Bagnulo

et al.

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(7), P. 726 - 726

Published: July 4, 2024

Male infertility accounts for approximately 40% of cases. There are many causes male infertility, including environmental factors, age, lifestyle, infections, varicocele, and cancerous pathologies. Severe oligozoospermia, cryptozoospermia, azoospermia (obstructive non-obstructive) identified as severe factor once considered conditions sterility. Today, in vitro fertilization (IVF) techniques the only treatment strategy cases which new methodologies have been developed manipulation spermatozoa to achieve increase success rates. This review is an update techniques, particular sperm selection, emphasizing clinical case-specific methodology. The IVF process related diagnosis, appropriate choice treatment, effective preparation selection. In fact, selecting best guarantee optimal paternal heritage means increasing blastulation, implantation, ongoing pregnancy live birth rates, resulting greater techniques.

Language: Английский

Citations

4
In vitro culture of cynomolgus monkey embryos from blastocyst to early organogenesis DOI

Yanhong Xu,

Jinglei Zhai, Hao Wu

et al.

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(12), P. 3677 - 3696

Published: July 25, 2024

Language: Английский

Citations

4