Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
Biology, Journal Year: 2025, Volume and Issue: 14(5), P. 521 - 521
Published: May 8, 2025
Cilia are evolutionarily conserved, microtubule-based organelles characterized by their ultrastructures and diverse functional roles, including developmental signaling, mechanosensation, fluid propulsion. They widely distributed across cell surfaces play crucial roles in cycle regulation tissue homeostasis. Despite advances studying molecular functions, demonstrating the precise ultrastructure of cilia remains a challenge. Recent novel microscopy techniques, such as super-resolution volume electron microscopy, revolutionizing our understanding architecture mechanochemical signaling. By integrating findings from different methodologies, this review highlights how these bridge basic research clinical applications provide comprehensive structural organization, mechanisms, dynamic changes cilia.
Language: Английский
Citations
0
medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: May 28, 2024
Abstract Primary ciliary dyskinesia (PCD) is a rare genetic disorder characterized by chronic respiratory tract infections and in some cases laterality defects infertility. The symptoms of PCD are caused malfunction motile cilia, hair-like organelles protruding out the cell that responsible for removal mucus from airways, organizing internal organ positioning during embryonic development gamete transport. mutations genes coding structural or assembly proteins cilia. Thus far, over 50 have been identified these variants explain around 70% all known cases. Population specific genetics underlying has reported underlining importance characterizing gene different populations gene-based diagnostics management. In this study, we disease causing axonemal central pair component HYDIN. Three Finnish patients carried homozygous loss-of-function one patient had compound heterozygous within HYDIN gene. functional effect was confirmed immunofluorescence electron tomography, which demonstrated complex. All clinical including wet cough recurrent airway due to almost static Our results consistent with previously important role complex improve ethnical backgrounds.
Language: Английский
Citations
2
Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)
Published: Oct. 14, 2024
ARL13B is a small regulatory GTPase that controls ciliary membrane composition in both motile cilia and non-motile primary cilia. In this study, we investigated the role of efferent ductules, tubules male reproductive tract essential to fertility which co-exist. We used genetically engineered mouse model delete Arl13b ductule epithelial cells, resulting compromised architecture functions. This deletion led disturbances reabsorptive/secretory processes triggered an inflammatory response. The observed phenotype showed significant variability linked partial infertility, highlighting importance maintaining proper physiological balance these ducts. These results emphasize dual functions regulating duct homeostasis, offering deeper insights into how related diseases affect system. disrupts structure leading impaired inflammation, subfertility. highlights ARL13B's critical physiology.
Language: Английский
Citations
2
Current Opinion in Endocrine and Metabolic Research, Journal Year: 2024, Volume and Issue: 34, P. 100509 - 100509
Published: Feb. 20, 2024
Language: Английский
Citations
2
Molecular Reproduction and Development, Journal Year: 2022, Volume and Issue: 90(1), P. 3 - 13
Published: Dec. 27, 2022
Abstract The biological events associated with mammalian reproductive processes are highly dynamic and tightly regulated by molecular, genetic, biomechanical factors. Implementation of live imaging in research is vital for the advancement our understanding normal physiology improving management disorders. Optical coherence tomography (OCT) emerging as a promising tool volumetric various mice other animal models. In this review, we summarize recent studies employing OCT‐based approaches toward investigation both, males females. We describe how OCT can be applied to study structural features male system sperm transport through tract. review applications vitro vivo female system, staging tracking oocytes embryos, investigations oocyte/embryo oviduct. functional approach analysis cilia dynamics within systems. also discuss areas research, where could find potential progress
Language: Английский
Citations
9
FEBS Journal, Journal Year: 2023, Volume and Issue: 291(19), P. 4159 - 4192
Published: Nov. 24, 2023
Multiciliated cells (MCCS) form bundles of cilia and their activities are essential for the proper development physiology many organ systems. Not surprisingly, defects in MCCs have profound consequences associated with numerous disease states. Here, we discuss current understanding MCC formation, a special focus on genetic molecular mechanisms fate choice differentiation. Furthermore, cast spotlight use zebrafish to study ontogeny several recent advances made using this vertebrate model delineate emergence developing kidney.
Language: Английский
Citations
4
Cells, Journal Year: 2024, Volume and Issue: 13(21), P. 1749 - 1749
Published: Oct. 23, 2024
Multiciliated cells (MCCs) serve many important functions, including fluid propulsion and chemo- mechanosensing. Diseases ranging from rare conditions to the recent COVID-19 global health pandemic have been linked MCC defects. In years, zebrafish has emerged as a model investigate biology of MCCs. Here, we review major events in formation centriole biogenesis basal body docking. Then, discuss studies on role MCCs diseases brain, respiratory, kidney reproductive systems, well findings about link between SARS-CoV-2. Next, explore why is useful study provide comprehensive overview previous genetic components essential for development motility across three tissues zebrafish: pronephros, brain ependymal nasal placode. Taken together, here cohesive summary research using its future potential expanding our understanding MCC-related disease states.
Language: Английский
Citations
1
Cells, Journal Year: 2024, Volume and Issue: 13(23), P. 1974 - 1974
Published: Nov. 28, 2024
Motile cilia are evolutionarily conserved organelles. In humans, multiciliated cells (MCCs), assembling several hundred motile on their apical surface, components of the monolayer epithelia lining lower and upper airways, brain ventricles, parts reproductive tracts, fallopian tube uterus in females, efferent ductules males. The coordinated beating generates a force that enables shift tubular fluid, particles, or along surface ciliated epithelia. Uncoordinated altered motion immotility may result subfertility even infertility. Here, we summarize current knowledge regarding localization function MCCs human discuss how beating-generated fluid flow directly indirectly contribute to processes these organs, lack improper functioning influence fertility.
Language: Английский
Citations
1
Food and Chemical Toxicology, Journal Year: 2023, Volume and Issue: 182, P. 114085 - 114085
Published: Oct. 14, 2023
Language: Английский
Citations
3
Biomedical Optics Express, Journal Year: 2022, Volume and Issue: 13(6), P. 3672 - 3672
Published: May 25, 2022
Spermatozoa transport within the male reproductive tract is a highly dynamic and biologically important event. However, due to lack of live volumetric imaging technologies quantitative measurements, there little information on aspect regulation this process. Here, we presented ex vivo mouse testis, efferent duct, epididymis, vas deferens at micro-scale spatial resolution with optical coherence tomography (OCT). Micro computed as reference for proposed OCT imaging. Application functional analysis allowed 3D mapping cilia beat frequency in which volumetrically visualized distribution ciliated cells corresponding ciliary activities. Potentially these analyses could be expanded settings through intravital approach. In summary, study demonstrated that has great potential investigate microstructure dynamics, such beating, muscle contractions, sperm transport, tract.
Language: Английский
Citations
4