Ethylene induced AcNAC3 and AcNAC4 take part in ethylene synthesis through mediating AcACO1 during kiwifruit (Actinidia chinensis) ripening DOI
Changchun Fu, Chao Han, Zuolong Yu

et al.

Journal of the Science of Food and Agriculture, Journal Year: 2024, Volume and Issue: 104(12), P. 7367 - 7374

Published: April 25, 2024

Abstract BACKGROUND Ethylene plays a vital role in the ripening process of kiwifruit. A terrific amount transcription factors (TFs) have been shown to regulate ethylene synthesis various fruits. RESULTS In this research, two new NAC TFs, named AcNAC3 and AcNAC4, were isolated from kiwifruit, which belonged NAM subfamily. Bioinformatics analysis showed that both AcNAC4 hydrophilic proteins with similar three‐dimensional structures. The expression levels , AcACO1 increased during kiwifruit ripening, as well induced by repressed 1‐methylcyclopropene (1‐MCP). Correlation exhibited production was positively correlated . Moreover, acted transcriptional activators could bind activate promoter. CONCLUSION All results unveiled ethylene‐induced activators, might participate biosynthesis through activating providing insight synthetic regulation ripening. © 2024 Society Chemical Industry.

Language: Английский

Transcriptional Control of Seed Life: New Insights into the Role of the NAC Family DOI Open Access
Javier Aguilar, Ángel J. Matilla

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5369 - 5369

Published: May 14, 2024

Transcription factors (TFs) regulate gene expression by binding to specific sequences on DNA through their DNA-binding domain (DBD), a universal process. This update conveys information about the diverse roles of TFs, focusing NACs (NAM-ATAF-CUC), in regulating target-gene and influencing various aspects plant biology. NAC TFs appeared before emergence land plants. The family constitutes group plant-specific found mosses, conifers, monocots, eudicots. discusses evolutionary origins genes/proteins from green algae crucial development stress response across species. From mosses lycophytes angiosperms, number proteins increases significantly, suggesting gradual evolution basal streptophytic algae. play critical role enhancing abiotic tolerance, with function conserved angiosperms. Furthermore, modular organization NACs, dimeric function, localization within cellular compartments contribute functional versatility complexity. While most are nuclear-localized active, subset is other compartments, indicating inactive forms until cues trigger translocation nucleus. Additionally, it highlights involvement endoplasmic reticulum (ER) stress-induced programmed cell death (PCD) activating vacuolar processing enzyme (VPE) gene. Moreover, this provides comprehensive overview plants, including participation ER responses, leaf senescence (LS), growth development. Notably, exhibit correlations phytohormones (i.e., ABA, GAs, CK, IAA, JA, SA), several genes inducible them, broad spectrum biological processes. study spatiotemporal patterns insights into when where shedding light metabolic contributions. Likewise, review emphasizes significance transcriptional modules, seed reserve accumulation, regulation dormancy germination. Overall, effectively communicates intricate essential functions Finally, an standpoint, phylogenetic analysis suggests that highly probable WRKY evolutionarily older than family.

Language: Английский

Citations

7
Tomato NAC transcription factor NOR-like1 positively regulates tomato fruit softening DOI
Zhenzhen Peng, Hongli Li,

Gangshuai Liu

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 213, P. 112923 - 112923

Published: April 3, 2024

Language: Английский

Citations

6
The MdNAC72‐MdABI5 module acts as an interface integrating jasmonic acid and gibberellin signals and undergoes ubiquitination‐dependent degradation regulated by MdSINA2 in apple DOI

Hong‐Liang Li,

Ruirui Xu,

Xin‐Long Guo

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(3), P. 997 - 1016

Published: June 7, 2024

Jasmonic acid (JA) and gibberellin (GA) coordinately regulate plant developmental programs environmental cue responses. However, the fine regulatory network of cross-interaction between JA GA remains largely elusive. In this study, we demonstrate that MdNAC72 together with MdABI5 positively regulates anthocyanin biosynthesis through an exquisite MdNAC72-MdABI5-MdbHLH3 transcriptional cascade in apple. interacts to promote activation on its target gene MdbHLH3 directly activates transcription MdABI5. The MdNAC72-MdABI5 module integration signals by combining repressor MdJAZ2 MdRGL2a. disrupts interaction attenuates MdNAC72. MdRGL2a sequesters from MdJAZ2-MdNAC72 protein complex, leading release E3 ubiquitin ligase MdSINA2 is responsive promotes ubiquitination-dependent degradation interface fine-regulates at posttranslational levels MdJAZ2, MdRGL2a, MdSINA2. summary, our findings elucidate connecting as core

Language: Английский

Citations

6
miR164–NAC21/22 module regulates the resistance of Malus hupehensis against Alternaria alternata by controlling jasmonic acid signaling DOI
Tingting Zhou, Lifang Cao, Kaixu Hu

et al.

Plant Science, Journal Year: 2023, Volume and Issue: 330, P. 111635 - 111635

Published: Feb. 12, 2023

Language: Английский

Citations

15
Phosphorylation of birch BpNAC90 improves the activation of gene expression to confer drought tolerance DOI Creative Commons
Zhibo Wang, Zihang He, Caiqiu Gao

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(4)

Published: Feb. 28, 2024

The NAC transcription factors (TFs) play important roles in mediating abiotic stress tolerance; however, the mechanism is still not fully known. Here, an gene (BpNAC90) from a regulatory network of Betula platyphylla (birch) that responded to drought was characterized. Overexpression and knockout BpNAC90 displayed increased reduced tolerance, respectively, relative wild-type (WT) birch. binds different DNA motifs regulate target genes conferring such as Eomes2, ABRE Tgif2. phosphorylated by at Ser 205 birch SNF1-related protein kinase 2 (BpSRK2A). Mutated (termed S205A) with abolished phosphorylation, transformed into for overexpression. transgenic S205A plants significantly tolerance compared overexpressing BpNAC90, but showed WT At same time, decreased capability bind activation expression, which contributed tolerance. Additionally, BpSRK2A can be induced form complex phosphorylate BpNAC90. results together indicated phosphorylation necessary

Language: Английский

Citations

5
Characteristics of NAC transcription factors in Solanaceae crops and their roles in responding to abiotic and biotic stresses DOI

Xiaogang Ou,

Lixinyu Sun,

Yu Chen

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 709, P. 149840 - 149840

Published: March 28, 2024

Language: Английский

Citations

5
A tomato NAC transcription factor, SlNAP1, directly regulates gibberellin-dependent fruit ripening DOI Creative Commons
Changxia Li, Xuemei Hou,

Zongxi Zhao

et al.

Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)

Published: April 23, 2024

In tomato (Solanum lycopersicum), the ripening of fruit is regulated by selective expression ripening-related genes, and this procedure controlled transcription factors (TFs). various plant-specific TF families, no apical meristem (NAM), Arabidopsis thaliana activating factor 1/2 (ATAF1/2), cup-shaped cotyledon 2 (CUC2; NAC) family stands out plays a significant function in plant physiological activities, such as (FR). Despite numerous genes NAC found genome, limited information available on effects members FR, there also lack studies their target genes. research, we focus SlNAP1, which that positively influences FR tomato. By employing CRISPR/Cas9 technology, compared with wild type (WT), generated slnap1 mutants observed delay ethylene production color change fruits. We employed yeast one-hybrid (Y1H) dual-luciferase reporter (DLR) assays to confirm SlNAP1 directly binds promoters two crucial involved gibberellin (GA) degradation, namely SlGA2ox1 SlGA2ox5, thus expression. Furthermore, through two-hybrid (Y2H), bimolecular fluorescence complementation (BIFC) luciferase (LUC) assays, established an interaction between SlGID1. Hence, our findings suggest regulates GA degradation directly. Additionally, SlGID1 may play role SlNAP1-induced FR. Overall, study provides important insights into molecular mechanisms TFs regulate via pathway.

Language: Английский

Citations

5
Genome-wide identification and comprehensive analyses of NAC transcription factor gene family and expression analysis under Fusarium kyushuense and drought stress conditions in Passiflora edulis DOI Creative Commons
Qiang Yang, Binqi Li, Hafiz Muhammad Rizwan

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Aug. 25, 2022

The NAC gene family is one of the largest plant transcription factors (TFs) families and plays important roles in growth, development, metabolism, biotic abiotic stresses. However, has not been reported passion fruit (Passiflora edulis). In this study, a total 105 genes were identified genome unevenly distributed across all nine-passion chromomere, with maximum 48 PeNAC on chromosome one. physicochemical features varied including 120 to 3,052 amino acids, 3 8 conserved motifs, 1 introns. named (PeNAC001-PeNAC105) according their chromosomal locations phylogenetically grouped into 15 clades (NAC-a NAC-o). Most proteins predicted be localized nucleus. cis-element analysis indicated possible light, hormones, stress responsiveness. Moreover, duplications tandem (11 pairs) segmental (12 subjected purifying selection. All exhibited similar 3D structures, protein-protein interaction network known Arabidopsis was predicted. Furthermore, 17 putative ped-miRNAs target 25 genes. Potential TFs ERF, BBR-BPC, Dof, bZIP promoter region visualized TF regulatory network. GO KEGG annotation exposed that related different biological, molecular, cellular terms. qRT-PCR expression discovered most PeNAC001, PeNAC003, PeNAC008, PeNAC028, PeNAC033, PeNAC058, PeNAC063, PeNAC077 significantly upregulated under Fusarium kyushuense drought conditions compared controls. conclusion, these findings lay foundation for further functional studies facilitate genetic improvement plants resistance.

Language: Английский

Citations

20
Transcriptional regulation of tomato fruit quality DOI
Hongli Li,

Gangshuai Liu,

Daqi Fu

et al.

Postharvest Biology and Technology, Journal Year: 2023, Volume and Issue: 202, P. 112393 - 112393

Published: May 16, 2023

Language: Английский

Citations

12
Molecular regulation of apple and grape ripening: exploring common and distinct transcriptional aspects of representative climacteric and non-climacteric fruits DOI Creative Commons
Sara Zenoni, Stefania Savoi, Nicola Busatto

et al.

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(20), P. 6207 - 6223

Published: Aug. 17, 2023

Abstract Fleshy fruits of angiosperms are organs specialized for promoting seed dispersal by attracting herbivores and enticing them to consume the organ seeds it contains. Ripening can be broadly defined as processes serving a plant strategy make fleshy fruit appealing animals, consisting coordinated series changes in color, texture, aroma, flavor that result from an intricate interplay genetically epigenetically programmed events. The ripening categorized into two types: climacteric, which is characterized rapid increase respiration rate typically accompanied burst ethylene production, non-climacteric, this pronounced peak absent. Here we review current knowledge transcriptomic taking place apple (Malus × domestica, climacteric) grapevine (Vitis vinifera, non-climacteric) during ripening, with aim highlighting specific common hormonal molecular events governing process species. With perspective, found NAC transcription factor members participate initiation grape involved restoring normal physiological progression impaired apple. These elements suggest existence regulatory mechanism operated factors auxin

Language: Английский

Citations

12