Process Biochemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 1, 2024
Language: Английский
Chemical Research in Toxicology, Journal Year: 2024, Volume and Issue: 37(2), P. 208 - 211
Published: Jan. 8, 2024
The Cell Counting Kit-8 (CCK-8) cell viability assay, also known as WST-8, is widely recognized for its nontoxic nature, making it suitable further studies on treated cells. This practice commonly observed in the field of tissue engineering. While live/dead imaging may not readily reveal macroscopic differences, our investigation has uncovered significant intracellular metabolic changes. Notably, we substantial down-regulation metabolites within glycolysis and pentose phosphate pathways. These alterations predominantly affect energy metabolism potentially impact cellular redox environment. In light these findings, strongly recommend that researchers exercise caution when using cells with CCK-8 subsequent experiments.
Language: Английский
Citations
11
Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)
Published: Jan. 4, 2025
CXCL14 is a highly conserved chemokine expressed in various cell types, playing crucial roles both physiological and pathological processes, including immune regulation tumorigenesis. Recently, the role of tumors has attracted considerable attention. However, previous pan-cancer studies have reported inconsistencies regarding effects on tumors, particularly concerning its expression levels tumor tissues influence phenotypes cancer cells. This variability believed to stem from context-dependent nature CXCL14, as different sources secretion within distinct microenvironments may mediate diverse biological effects. Such phenomena also been observed prostate research. Despite foundational understanding cancer, there remains lack comprehensive reviews summarizing specific this systematically analyzing reasons behind complex Therefore, article aims discuss microenvironment explore future research directions potential applications.
Language: Английский
Citations
1
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 263, P. 130371 - 130371
Published: Feb. 27, 2024
Language: Английский
Citations
8
Electrophoresis, Journal Year: 2024, Volume and Issue: 45(17-18), P. 1562 - 1573
Published: May 13, 2024
Abstract Human mesenchymal stem cells (hMSCs) have gained traction in transplantation therapy due to their immunomodulatory, paracrine, immune‐evasive, and multipotent differentiation potential. The inherent heterogeneity of hMSCs poses a challenge for therapeutic treatments necessitates the identification robust biomarkers ensure reproducibility both vivo vitro experiments. In this study, we utilized dielectrophoresis (DEP), label‐free electrokinetic phenomenon, investigate derived from bone marrow (BM) adipose tissue (AD). electrical properties BM‐hMSCs were compared homogeneous mouse fibroblasts (NIH‐3T3), human (WS1), embryonic kidney (HEK‐293). DEP profile differed most HEK‐293 cells. We profiles AD‐hMSCs found that they similar membrane capacitances, differing cytoplasm conductivity, transient slopes. Inducing populations differentiate into adipocyte osteoblast revealed behave differently response differentiation‐inducing cytokines. Histology reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) analyses differentiation‐related genes differences between AD‐hMSCs. correlate well with developed indicate Our results demonstrate using DEP, capacitance, slope can uniquely characterize guide reproducible cell therapies.
Language: Английский
Citations
7
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 485, P. 149849 - 149849
Published: Feb. 20, 2024
Aerogel technology is an emerging platform that offers alternative and cost-effective dry carriers with advanced performances for the pharmaceutical industry. The innovative combination of compressed air-assisted spray gelation ''green'' supercritical fluid (SCF) was used in this study to produce alginate aerogel microparticles adequate properties pulmonary drug delivery. Beclomethasone dipropionate (BDP), a poorly water-soluble anti-inflammatory asthma treatment, loaded into particles by SCF-assisted impregnation ensuring high loading, as well amorphization API. production aerogels optimized through fine-tuning parameters gelation, specifically adjusting air flow rate pump speed. Alginate were aimed be produced appropriate aerodynamic diameter from 1 5 µm, measured vitro deposition tests Next Generation impactor potential deep lung penetration. Nuclear magnetic resonance (NMR) relaxometry assess hydration unveiled structure–property relationships leading sudden release drug. Finally, cytotoxicity fibroblasts ex vivo permeability conducted. These biological confirmed excellent biocompatibility formulations demonstrated efficient BDP porcine bronchial tissues assisted porous carrier.
Language: Английский
Citations
6
EMBO Molecular Medicine, Journal Year: 2024, Volume and Issue: 16(4), P. 1027 - 1045
Published: March 6, 2024
Abstract Clinical deployment of oligonucleotides requires delivery technologies that improve stability, target tissue accumulation and cellular internalization. Exosomes show potential as ideal vehicles. However, an affordable generalizable system for efficient loading on exosomes remain lacking. Here, we identified Exosomal Anchor DNA Aptamer (EAA) via SELEX against immobilized with our proprietary CP05 peptides. EAA shows high binding affinity to different enables nucleic acid drugs exosomes. Serum stability thrombin inhibitor NU172 was prolonged by exosome-loading, resulting in increased blood flow after injury vivo. Importantly, Duchenne Muscular Dystrophy PMO can be readily loaded (EXO EAA-PMO ). EXO elicited significantly greater muscle cell uptake, dystrophin expression than vitro Systemic administration therapeutic levels restoration functional improvements mdx mice. Altogether, study demonstrates exosomes, thus providing easy strategy therapeutics
Language: Английский
Citations
5
Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1318, P. 139338 - 139338
Published: July 17, 2024
Language: Английский
Citations
5
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 277, P. 134565 - 134565
Published: Aug. 6, 2024
Chronic wounds represent a serious worldwide concern, being often associated with bacterial infections. As the prevalence of infections increase, it is crucial to search for alternatives. Essential oils (EOs) constitute promising option antibiotics due their strong anti-inflammatory, analgesic, antioxidant and antibacterial properties. However, such compounds present high volatility. To address this issue, drug delivery system composed coaxial wet-spun fibers was engineered different EOs, namely clove oil (CO), cinnamon leaf (CLO) tea tree (TTO), were loaded. Briefly, two syringe pumps, coagulation bath deionized water, cylindrical-shaped collector spinneret used. A 10 % w/v polycaprolactone (PCL) solution combined EOs at 2 × minimum bactericidal concentration (MBC) loaded connected inner port, whereas cellulose acetate (CA) mixed polyethylene glycol (PEG) ratio 90:10 v/v (to increase fibers' elasticity) outer port. This layer used as barrier pace release entrapped EO. The CA's inherent porosity in water baths allowed access fiber's core. CA also (CA:PEG), elasticity. Microfibers maintained structural integrity during 28 days incubation physiological-like environments. They showed elasticities (maximum elongations break >300 %) resistance rupture mechanical assessments, reaching mass losses only ≈ 2.29 - 57.19 %. released from prolonged sustained fashion, which 30 EO 24 h media, demonstrating great effectiveness against Staphylococcus aureus, epidermidis, Escherichia coli Pseudomonas aeruginosa, most prevalent bacteria chronic wounds. Moreover, microfibers effective effects, presenting up 59 reduction 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity. Furthermore, deemed safe contact fibroblasts human keratinocytes, metabolic activities higher than 80 after 48 incubation. Data confirmed suitability potential therapeutics
Language: Английский
Citations
5
Materials Advances, Journal Year: 2023, Volume and Issue: 4(22), P. 5618 - 5632
Published: Jan. 1, 2023
The development of surface technologies to obtain improved tribomechanical and biological characteristics synthetic NiTi implants is critical.
Language: Английский
Citations
12
European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 287, P. 117364 - 117364
Published: Feb. 5, 2025
Language: Английский
Citations
0