Platelet-rich fibrin as an autologous biomaterial for bone regeneration: mechanisms, applications, optimization

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: April 16, 2024

Platelet-rich fibrin, a classical autologous-derived bioactive material, consists of fibrin scaffold and its internal loading growth factors, platelets, leukocytes, with the gradual degradation slow release physiological doses factors. PRF promotes vascular regeneration, proliferation migration osteoblast-related cells such as mesenchymal cells, osteoblasts, osteoclasts while having certain immunomodulatory anti-bacterial effects. has excellent osteogenic potential been widely used in field bone tissue engineering dentistry. However, there are still some limitations PRF, improvement biological properties is one most important issues to be solved. Therefore, it often combined scaffolds enhance mechanical delay degradation. In this paper, we present systematic review development platelet-rich derivatives, structure mechanisms, applications, optimization broaden their clinical applications provide guidance for translation.

Language: Английский

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How to explain the beneficial effects of platelet‐rich plasma

Periodontology 2000, Journal Year: 2024, Volume and Issue: unknown

Published: April 10, 2024

Abstract Platelet‐rich plasma (PRP) is the platelet and leukocyte‐containing plasmatic fraction of anticoagulated autologous blood. While evidence supporting clinical use PRP in dentistry low, widely used sports medicine, orthopedics, dermatology. Its beneficial activity commonly attributed to growth factors released from platelets accumulating PRP; however, indirect not comprehensive. There thus a demand revisit with respect basic translational science. This review (i) recapitulate protocols tools prepare (ii) discuss cellular molecular composition focus on platelets, leukocytes, fibrin‐rich extracellular matrix coagulated plasma; finally (iii) potential effects level an outlook its current other medical fields.

Language: Английский

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How to explain the beneficial effects of leukocyte‐ and platelet‐rich fibrin

Periodontology 2000, Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

The survival of an organism relies on its ability to repair the damage caused by trauma, toxic agents, and inflammation. This process involving cell proliferation differentiation is driven several growth factors critically dependent organization extracellular matrix. Since autologous platelet concentrates (APCs) are fibrin matrices in which cells, factors, cytokines trapped delivered over time, they able influence that response at different levels. present review thoroughly describes molecular components one these APCs, leukocyte- platelet-rich (L-PRF), summarizes level evidence regarding L-PRF anti-inflammatory reactions, analgesia, hemostasis, antimicrobial capacity, biological mechanisms bone/soft tissue regeneration.

Language: Английский

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Effect of calcium carbonate nanoparticles, silver nanoparticles and advanced platelet-rich fibrin for enhancing bone healing in a rabbit model

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 14, 2023

Abstract This study aimed to evaluate the efficacy of calcium carbonate nanoparticles (CCNPs) induce new bone formation in a critical size segmental defect rabbit’s radius when used alone, combined with silver (AgNPs) as paste, or composite containing CCNPs, AgNPs, and advanced platelet-rich fibrin (A-PRF). Thirty-six adult apparently healthy male New Zealand White rabbits aging from 5 6 months weighting 3.5 ± 0.5 kg were used. The animals divided into four groups; control group, CCNPs CCNPs/AgNPs paste CCNPs/AgNPs/A-PRF group. investigated at 4, 8, 12 weeks post-implantation which healing was evaluated using computed tomographic (CT) histopathological evaluation. results revealed that has superior effect regarding amount quality newly formed compared alone. In conclusion, addition AgNPs and/or A-PRF reduced its resorption rate improved osteogenic osteoinductive properties.

Language: Английский

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Bone Formation and Maintenance in Oral Surgery: The Decisive Role of the Immune System—A Narrative Review of Mechanisms and Solutions

Bioengineering, Journal Year: 2024, Volume and Issue: 11(2), P. 191 - 191

Published: Feb. 16, 2024

Based on the evidence of a significant communication and connection pathway between bone immune systems, new science has emerged: osteoimmunology. Indeed, system considerable impact health diseases, as well formation during grafts its stability over time. Chronic inflammation induces excessive production oxidants. An imbalance levels oxidants antioxidants is called oxidative stress. This physio-pathological state causes both molecular cellular damage, which leads to DNA alterations, genetic mutations cell apoptosis, thus, impaired immunity followed by delayed or compromised wound healing. Oxidative stress experienced body affect regeneration maintenance around teeth dental implants. As remodeling are interconnected, loss consequence dysregulation. Therefore, oral tissue deficiencies such periodontitis peri-implantitis should be regarded diseases. Bone management strategies include biological surgical solutions. These protocols tend improve through antioxidant enhance prevent loss. narrative review aims highlight relationship inflammation, oxidation, in cavity. It intends help clinicians detect high-risk situations surgery propose clinical solutions that will patients’ responses treatment outcomes.

Language: Английский

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Oral cell lysates reduce osteoclastogenesis in murine bone marrow cultures

Cytotechnology, Journal Year: 2025, Volume and Issue: 77(1)

Published: Jan. 8, 2025

Abstract Mechanical and thermal cell damage can occur due to invasive procedures related drilling, the insertion of dental implants, periodontal treatments. Necrotic cells release content their cytoplasm membrane fragments, thereby signaling need for repair, which includes bone resorption by osteoclasts inflammation. Here we screened lysates from human gingival fibroblasts, HSC2 TR146 oral squamous carcinoma lines, as well murine IDG-SW3 osteocytic RAW264.7 macrophage lines potential modulate in vitro osteoclastogenesis marrow cultures. We also tested impact necrotic on modulating expression inflammatory cues ST2 stromal cells. report here that independent or origin, all significantly reduced cultures, indicated osteoclast marker genes cathepsin K tartrate-resistant acid phosphatase respective histochemical staining multinucleated found pushed CCL2, CCL5, CXCL1, IL1, IL6 These findings suggest reduce osteoclastogenesis, but only damaged force produce an environment.

Language: Английский

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RNAseq of peripheral blood mononucleated cells exposed to platelet-rich fibrin and enamel matrix derivatives

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 29, 2025

Platelet-rich fibrin (PRF) and Enamel Matrix Derivatives (EMD) can support the local regenerative events in periodontal defects. There is reason to suggest that PRF EMD exert part of their activity by targeting blood-derived cells accumulating early wound healing blastema. However, impact on blood cell response remains be discovered. To this aim, we have exposed human peripheral mononucleated (PBMCs) lysates prepared a swing-out rotor EMD, followed bulk RNA sequencing. A total 111 8 genes are up- down-regulated under premise an at least log2 two-fold change minus log10 significance level two, respectively. Representative characteristic IFN indicated various leukocyte antigens (HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQA2, HLA-DRA, HLA-DRB1, HLA-DRB5), gamma Fc receptors (FCGR1A, FCGR1B, FCGR3B), chemokines (CXCL9-11), calprotectin (S100A8/9 S100A12), complement (C1QA/B, C2) interferon-induced guanylate-binding proteins (GBP1, GBP5). With 67 29 down-regulated, Characteristic upregulated tensins (TNS1 TNS3). Among downregulated were epsilon (FCER1A; FCER2), receptor-like (FCRL1, FCRL3) CX3CR1. Genes commonly most noticeably NXPH4 MN1, as well FN1, MMP14, MERTK, AXL. Our findings provokes inflammatory response, while dampens IgE signaling cells.

Language: Английский

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Regenerative effect of lyophilized dental follicle mesenchymal stem cells and platelet-rich fibrin in skin wounds in geriatric and young rats

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 24, 2025

The aim of this study was to investigate the regenerative effect lyophilized dental follicle mesenchymal stem cells (DF-MSCs) combined with rat platelet-rich fibrin (PRF) on geriatric skin wounds. Human DF-MSCs which were isolated from wisdom teeth healthy donors and PRF mixed incubated in a 37 °C incubator for 1–2 h containing 1 million 150 mg PRF. mixture suspended freeze-drying solution then lyophilized. Wounds created back Wistar albino rats using 6 mm punch. Lyophilized DF-MSCs, PRF, or + applied wounds rats. On 15th day, wound area histopathologically evaluated Blood samples analyzed total antioxidant status (TAOS), inflammatory cytokine levels ELISA. In both young treated began significantly decrease 10th day compared untreated group (p < 0.05). Histopathological examination revealed that groups, epithelial integrity scarless healing increased groups There no significant differences TAOS, oxidant (TOS), tumor necrosis factor (TNF), interleukin-6 (IL6), hydroxyproline serum day. rats, (HYPS) DF-MSC 0.01), TNF elevated IL6 control = 0.01). is shelf-stable ready-to-use product, hold promise, especially traumatic individuals longer times.

Language: Английский

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The Regenerative Marriage Between High-Density Platelet-Rich Plasma and Adipose Tissue

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2154 - 2154

Published: Feb. 27, 2025

The use of autologous biological preparations (ABPs) and their combinations fills the void in healthcare treatment options that exists between surgical procedures, like plastic reconstructive, cosmetic, orthopedic surgeries; non-surgical musculoskeletal procedures; current pharmaceutical treatments. ABPs, including high-density platelet-rich plasma (HD-PRP), bone marrow aspirate concentrates (BMACs), adipose tissue preparations, with unique stromal vascular fractions (SVFs), can play important roles regeneration repair processes. They be easily safely prepared at point care. Healthcare professionals employ ABPs to mimic classical wound healing cascade, initiate angiogenesis induce regenerative pathways, aiming restore integrity function damaged tissues. In this review, we will address combining HD-PRP tissue, particular fraction (t-SVF), as believe biocellular combination demonstrates a synergistic effect, where constituents enhance potential t-SVF its adipose-derived mesenchymal stem cells (AD-MSCs) pericytes, leading improved functional repair, regeneration, variety clinical applications. We some relevant platelet bio-physiological aspects, since these properties contribute effects t-SVF, promoting overall better outcomes chronic inflammatory conditions, soft rejuvenation.

Language: Английский

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Advantages of horizontal centrifugation of platelet‐rich fibrin in regenerative medicine and dentistry

Periodontology 2000, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

The aim of this comprehensive review was to evaluate comparative studies on horizontal and fixed-angle centrifugation methods for preparing platelet-rich fibrin (PRF). Furthermore, additional utilizing PRF (H-PRF) were systematically investigated. This overview article offers deeper insights into the advantages H-PRF when compared across a wide range regenerative medical dental applications. A search conducted in PubMed Web Science up December 5, 2024. Grey literature also searched via Google Scholar relevant studies, reference lists eligible screened further potential inclusion. All vitro, vivo, clinical that utilized or swing-out prepare solid liquid PRF, along with their subfractions such as buffy coat, platelet-poor plasma (PPP), heated variants like albumin gel (Alb-PRF) interventions, included study. total 75 included. Thirteen directly producing while remaining 62 non-comparative focused expanding uses applications H-PRF. These spanned categories cell concentrations, matrix structure, growth factor release, antibacterial anti-inflammatory properties, bone, periodontal, cartilage, skin, hair, endodontics, corneal defect repair, wound healing, soft tissue regeneration. Of comparing centrifugation, 84.6% favored 15.4% found no difference. None centrifugation. Additionally, more optimized concentrating liquid-PRF (C-PRF) using extending resorption properties PRF-ranging from 2 3 weeks membranes lasting 4 months through an denaturation process were-further discussed. Based these findings, it remains logical utilize practice owing greater superiority results majority studies. Nevertheless, are needed support findings. While current evidence is limited trials warranted, several have now indicated fixed-angle, higher uniform distribution, increased release. suggest use may lead enhanced outcomes application indicated. Since can better separation, should be preferred method C-PRF Alb-PRF

Language: Английский

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