Regeneration in Mice of Injured Skin, Heart, and Spinal Cord by α-Gal Nanoparticles Recapitulates Regeneration in Amphibians DOI Creative Commons
Uri Galili, Jianming Li, Gary L. Schaer

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(8), P. 730 - 730

Published: April 22, 2024

The healing of skin wounds, myocardial, and spinal cord injuries in salamander, newt, axolotl amphibians, mouse neonates, results scar-free regeneration, whereas adult mice heal by fibrosis scar formation. Although both types are mediated macrophages, regeneration these amphibians neonates also involves innate activation the complement system. These differences suggest that localized might induce instead default Localized is feasible antigen/antibody interaction between biodegradable nanoparticles presenting α-gal epitopes (α-gal nanoparticles) natural anti-Gal antibody which abundant humans. Administration into anti-Gal-producing induces rapid extensive macrophage recruitment. macrophages bind anti-Gal-coated polarize M2 pro-regenerative orchestrate accelerated wounds myocardium injured myocardial infarction (MI). Furthermore, injection recruitment mediate angiogenesis axonal sprouting, reconnects proximal distal severed axons. Thus, nanoparticle treatment mimics physiologic amphibians. studies further may be significance human injuries.

Language: Английский

Impact of inflammation and Treg cell regulation on neuropathic pain in spinal cord injury: mechanisms and therapeutic prospects DOI Creative Commons

Chunjia Zhang,

Yan Li, Yan Yu

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 29, 2024

Spinal cord injury is a severe neurological trauma that can frequently lead to neuropathic pain. During the initial stages following spinal injury, inflammation plays critical role; however, excessive exacerbate Regulatory T cells (Treg cells) have crucial function in regulating and alleviating Treg release suppressor cytokines modulate of other immune suppress inflammatory response. Simultaneously, impedes cell activity, further intensifying Therefore, suppressing response while enhancing regulatory may provide novel therapeutic avenues for treating pain resulting from injury. This review comprehensively describes mechanisms underlying regulation subsequent with specific focus on exploring potential through which regulate The insights gained this aim new concepts rationale prospects direction therapy injury-related conditions.

Language: Английский

Citations

15
Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury DOI Creative Commons

Dezun Ma,

Changlong Fu,

Fenglu Li

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 521 - 543

Published: May 30, 2024

Spinal cord injury (SCI) often results in irreversible loss of sensory and motor functions, most SCIs are incurable with current medical practice. One the hardest challenges treating SCI is development a dysfunctional pathological microenvironment, which mainly comprises excessive inflammation, deposition inhibitory molecules, neurotrophic factor deprivation, glial scar formation, imbalance vascular function. To overcome this challenge, implantation functional biomaterials at site has been regarded as potential treatment for modulating microenvironment to support axon regeneration, remyelination site, recovery after SCI. This review summarizes characteristics recent advances well technologies used modulate inflammatory regulate reshape revascularization microenvironment. Moreover, technological limitations, challenges, future prospects promote efficient repair also discussed. will aid further understanding

Language: Английский

Citations

13
The roles of neural stem cells in myelin regeneration and repair therapy after spinal cord injury DOI Creative Commons
Chun Li, Yuping Luo, Siguang Li

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 8, 2024

Abstract Spinal cord injury (SCI) is a complex tissue that results in wide range of physical deficits, including permanent or progressive disabilities sensory, motor and autonomic functions. To date, limitations current clinical treatment options can leave SCI patients with lifelong disabilities. There an urgent need to develop new therapies for reconstructing the damaged spinal neuron-glia network restoring connectivity supraspinal pathways. Neural stem cells (NSCs) possess ability self-renew differentiate into neurons neuroglia, oligodendrocytes, which are responsible formation maintenance myelin sheath regeneration demyelinated axons. For these properties, NSCs considered be promising cell source rebuilding neural circuits promoting regeneration. Over past decade, transplantation has been extensively tested variety preclinical models SCI. This review aims highlight pathophysiology promote understanding role repair therapy advances pathological mechanism, pre-clinical studies, as well trials via NSC therapeutic strategy. Understanding mastering frontier updates will pave way establishing novel strategies improve quality recovery from

Language: Английский

Citations

11
Albiflorin Attenuates Neuroinflammation and Improves Functional Recovery After Spinal Cord Injury Through Regulating LSD1-Mediated Microglial Activation and Ferroptosis DOI Creative Commons
Longyu Zhang,

Jiao Xu,

Shi Yin

et al.

Inflammation, Journal Year: 2024, Volume and Issue: 47(4), P. 1313 - 1327

Published: Feb. 10, 2024

Spinal cord injury (SCI) is a serious, prolonged, and irreversible with few therapeutic options. Albiflorin (AF) possesses powerful pharmacodynamic properties exerts protective effects against neuroinflammation. However, no research has examined the neuroprotective effect of AF following SCI. Rats were received laminectomy to establish SCI animal model treated (20 mg/kg 40 mg/kg). Behavioral experiments conducted assess impacts on motor function after in rats. Hematoxylin-eosin (HE) staining, Nissl Prussian Blue staining performed observe histological changes, neuronal damage, iron deposition, respectively. Transmission electron microscope was adopted ultrastructure spinal tissues. Immunofluorescence assay examine neurons microglia. ELISA used production cytokines. Western blot detect expression level ferroptosis-related proteins. Microglia BV-2 cells induced by LPS mimic neuroinflammatory condition. Cell viability assessed CCK-8 assay, lipid peroxidase measured C11 BODIPY 581/591 staining. Molecular docking technology utilized confirm relationship between LSD1. improved functional recovery Meanwhile, attenuated neuron apoptosis microglia activation, reduced pro-inflammatory cytokines accumulation, inhibited ferroptosis LSD1 verified be target protein AF, could concentration-dependently downregulate injured cords vivo LPS-induced vitro. In addition, not only through reducing levels regulating proteins, but also microglial activation cells; however, these changes partly counteracted overexpression. reduce ferroptosis, attenuate neuroinflammation, improve downregulating LSD1, providing novel strategies for treatment

Language: Английский

Citations

10
Integrated bioinformatics analysis identified cuproptosis-related hub gene Mpeg1 as potential biomarker in spinal cord injury DOI Creative Commons
Mao Dandan, Qi Chen,

Shuolan Tong

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 15, 2025

Language: Английский

Citations

2
Neurotrauma—From Injury to Repair: Clinical Perspectives, Cellular Mechanisms and Promoting Regeneration of the Injured Brain and Spinal Cord DOI Creative Commons
Andrew R. Stevens, Antonio Belli, Zubair Ahmed

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 643 - 643

Published: March 13, 2024

Traumatic injury to the brain and spinal cord (neurotrauma) is a common event across populations often causes profound irreversible disability. Pathophysiological responses trauma exacerbate damage of an index injury, propagating loss function that central nervous system (CNS) cannot repair after initial resolved. The way in which lost consequence complex array mechanisms continue chronic phase post-injury prevent effective neural repair. This review summarises events traumatic (TBI) (SCI), comprising description current clinical management strategies, summary known cellular molecular secondary their role prevention A discussion emerging approaches promote neuroregeneration CNS presented. barriers promoting neurotrauma are pathways cell types occur on level. presents challenge traditional pharmacological targeting single pathways. It suggested novel multiple or using combinatorial therapies may yield sought-after recovery for future patients.

Language: Английский

Citations

9
Multilevel neurium-mimetic individualized graft via additive manufacturing for efficient tissue repair DOI Creative Commons
Lingchi Kong, Xin Gao, Xiangyun Yao

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 30, 2024

Abstract Complicated peripheral nerve injuries or defects, especially at branching sites, remain a prominent clinical challenge after the application of different treatment strategies. Current grafts fail to match expected shape and size for delicate precise branched repair on case-by-case basis, there is lack geometrical microscale regenerative navigation. In this study, we develop sugar painting-inspired individualized multilevel epi-/peri-/endoneurium-mimetic device (SpinMed) customize natural cues, featuring selectively protective outer sheath an instructive core, support rapid vascular reconstruction consequent efficient neurite extension along defect area. The biomimetic perineurium dictates host-guest crosslinking in which new vessels secrete multimerin 1 binding fibroin filler surface as anchor, contributing biological endoneurium that promotes Schwann cell homing remyelination. SpinMed implantation into rat sciatic defects yields satisfactory outcome terms structural reconstruction, with sensory locomotive function restoration. We further based anatomy digital imaging, achieving trunk branches superior achieved by autografts decellularized specific beagle model, reliable biosafety. Overall, intelligent art-inspired design offers facile way sophisticated high-performance holds great potential translational medicine.

Language: Английский

Citations

8
Targeting NMDA receptors with an antagonist is a promising therapeutic strategy for treating neurological disorders DOI
Md. Rajdoula Rafe, Pranoy Saha, Stephen Temitayo Bello

et al.

Behavioural Brain Research, Journal Year: 2024, Volume and Issue: 472, P. 115173 - 115173

Published: Aug. 1, 2024

Language: Английский

Citations

7
BDNF-GABA signaling in astrocytes: enhancing neural repair after SCI through MSC therapies DOI

Qingsheng Zhou,

Qiongxuan Fang,

Chunming Zhang

et al.

Spinal Cord, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

Language: Английский

Citations

1
How can clinical safety and efficacy concerns in stem cell therapy for spinal cord injury be overcome? DOI
Nader Hejrati, Raymond Wong, Mohammad Rasool Khazaei

et al.

Expert Opinion on Biological Therapy, Journal Year: 2023, Volume and Issue: 23(9), P. 883 - 899

Published: Aug. 7, 2023

Spinal cord injury (SCI) can lead to severe neurological dysfunction. Despite scientific and medical advances, clinically effective regenerative therapies including stem cells are lacking for SCI.This paper discusses translational challenges related the safe, use of SCI, with a focus on mesenchymal (MSCs), neural (NSCs), Schwann (SCs), olfactory ensheathing (OECs), oligodendrocyte precursor (OPCs), embryonic (ESCs), induced pluripotent (iPSCs). We discuss approaches enhance efficacy cell-based strategies by i) addressing patient heterogeneity enhancing selection; ii) selecting cell type, source, developmental stage, delivery technique; iii) graft integration mitigating immune-mediated rejection; iv) ensuring availability cells. Additionally, we review optimize outcomes combinatorial rehabilitation ways mitigate potential risks tumor formation associated strategies.Basic science research will drive advances develop SCI. Genetic, serological, imaging biomarkers may enable individualization treatments. Moreover, be required survival, migration functional integration, precision-based intervention.

Language: Английский

Citations

14