Exosome-Based Drug Delivery: Translation from Bench to Clinic

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(8), P. 2042 - 2042

Published: July 29, 2023

Exosome-based drug delivery is emerging as a promising field with the potential to revolutionize therapeutic interventions. Exosomes, which are small extracellular vesicles released by various cell types, have attracted significant attention due their unique properties and natural ability transport bioactive molecules. These nano-sized vesicles, ranging in size from 30 150 nm, can effectively variety of cargoes, including proteins, nucleic acids, lipids. Compared traditional systems, exosomes exhibit biocompatibility, low immunogenicity, reduced toxicity. In addition, be designed tailored improve targeting efficiency, cargo loading capacity, stability, paving way for personalized medicine precision therapy. However, despite exosome-based delivery, its clinical application remains challenging limitations exosome isolation purification, efficiency insufficient targeted rapid elimination circulation. This comprehensive review focuses on transition bench clinic, highlighting key aspects, such structure biogenesis, methods, surface engineering techniques, applications. It also discusses challenges prospects this field.

Language: Английский

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Therapeutic potential of RNA-enriched extracellular vesicles: The next generation in RNA delivery via biogenic nanoparticles

Molecular Therapy, Journal Year: 2024, Volume and Issue: 32(9), P. 2939 - 2949

Published: Feb. 27, 2024

Language: Английский

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Extracellular Vesicles and Exosomes: Novel Insights and Perspectives on Lung Cancer from Early Detection to Targeted Treatment

Biomedicines, Journal Year: 2024, Volume and Issue: 12(1), P. 123 - 123

Published: Jan. 7, 2024

Lung cancer demands innovative approaches for early detection and targeted treatment. In addressing this urgent need, exosomes play a pivotal role in revolutionizing both the treatment of lung cancer. Their remarkable capacity to encapsulate diverse range biomolecules, traverse biological barriers, be engineered with specific targeting molecules makes them highly promising diagnostic markers precise drug delivery cells. Furthermore, an in-depth analysis exosomal content biogenesis offers crucial insights into molecular profile tumors. This knowledge holds significant potential development therapies strategies Despite notable progress field, challenges standardization cargo loading persist. Collaborative research efforts are imperative maximize advance field precision medicine benefit patients.

Language: Английский

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Cancer Nanovaccines: Nanomaterials and Clinical Perspectives

Small, Journal Year: 2024, Volume and Issue: 20(35)

Published: May 1, 2024

Abstract Cancer nanovaccines represent a promising frontier in cancer immunotherapy, utilizing nanotechnology to augment traditional vaccine efficacy. This review comprehensively examines the current state‐of‐the‐art nanovaccine development, elucidating innovative strategies and technologies employed their design. It explores both preclinical clinical advancements, emphasizing key studies demonstrating potential elicit robust anti‐tumor immune responses. The study encompasses various facets, including integrating biomaterial‐based nanocarriers for antigen delivery, adjuvant selection, impact of nanoscale properties on performance. Detailed insights into complex interplay between tumor microenvironment responses are provided, highlighting challenges opportunities optimizing therapeutic outcomes. Additionally, presents thorough analysis ongoing trials, presenting snapshot landscape. By curating latest scientific findings developments, this aims serve as comprehensive resource researchers clinicians engaged advancing immunotherapy. Integrating design holds immense promise revolutionizing treatment paradigms, provides timely update evolving landscape nanovaccines.

Language: Английский

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Emerging roles of exosomes in oral diseases progression

International Journal of Oral Science, Journal Year: 2024, Volume and Issue: 16(1)

Published: Jan. 15, 2024

Abstract Oral diseases, such as periodontitis, salivary gland and oral cancers, significantly challenge health conditions due to their detrimental effects on patient’s digestive functions, pronunciation, esthetic demands. Delayed diagnosis non-targeted treatment profoundly influence patients’ prognosis quality of life. The exploration innovative approaches for early detection precise represents a promising frontier in medicine. Exosomes, which are characterized nanometer-sized extracellular vesicles, secreted by virtually all types cells. As the research continues, complex roles these intracellular-derived vesicles biological processes have gradually unfolded. Exosomes attracted attention valuable diagnostic therapeutic tools ability transfer abundant cargos intricate involvement multiple cellular functions. In this review, we provide an overview recent applications exosomes within field focusing inflammation-related bone diseases squamous cell carcinomas. We characterize exosome alterations demonstrate potential biomarkers diagnosis, highlighting indicators diseases. also summarize targeted therapy proposed future directions use clinical treatment.

Language: Английский

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Insight into the Functional Dynamics and Challenges of Exosomes in Pharmaceutical Innovation and Precision Medicine

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(6), P. 709 - 709

Published: May 24, 2024

Of all the numerous nanosized extracellular vesicles released by a cell, endosomal-originated exosomes are increasingly recognized as potential therapeutics, owing to their inherent stability, low immunogenicity, and targeted delivery capabilities. This review critically evaluates transformative of exosome-based modalities across pharmaceutical precision medicine landscapes. Because precise biomolecular cargo delivery, posited ideal candidates in drug enhancing regenerative strategies, advancing diagnostic technologies. Despite significant market growth projections exosome therapy, its utilization is encumbered substantial scientific regulatory challenges. These include lack universally accepted protocols for isolation complexities associated with navigating environment, particularly guidelines set forth U.S. Food Drug Administration (FDA). presents comprehensive overview current research trajectories aimed at addressing these impediments discusses prospective advancements that could substantiate clinical translation exosomal therapies. By providing analysis both capabilities hurdles therapeutic applications, this article aims inform direct future paradigms, thereby fostering integration systems into mainstream practice.

Language: Английский

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Natural and Bioengineered Extracellular Vesicles in Diagnosis, Monitoring and Treatment of Cancer

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Extracellular vesicles (EVs) are cell derived nanovesicles which implicated in both physiological and pathological intercellular communication, including the initiation, progression, metastasis of cancer. The exchange biomolecules between stromal cells cancer via EVs can provide a window to monitor development real time for better diagnostic interventional strategies. In addition, process secretion internalization by tumor microenvironment (TME) be exploited delivering therapeutics. have potential targeted, biocompatible, efficient delivery platform treatment other diseases. Natural as well engineered nanomedicine immense disease intervention. Here, we an overview current knowledge EVs' function therapeutic applications setting, EV engineering

Language: Английский

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Nature Inspired Delivery Vehicles for CRISPR‐Based Genome Editing

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract The advent of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)‐based genome editing technologies has opened up groundbreaking possibilities for treating a wide spectrum genetic disorders and diseases. However, the success these relies heavily on development efficient safe delivery systems. Among most promising approaches are natural synthetic nanocarrier‐mediated systems, including viral vectors, extracellular vesicles (EVs), engineered cellular membrane particles, liposomes, various nanoparticles. These carriers enhance efficacy CRISPR system by providing unique combination efficiency, specificity, reduced immunogenicity. Synthetic such as liposomes nanoparticles facilitate with high reproducibility customizable functions. Viral renowned their transduction efficiency broad tropism, serve powerful vehicles delivering components to cell types. EVs, RNA proteins, offer stealth mechanism evade immune detection, allowing targeted editors minimal off‐target effects. Engineered particles further improve simulating environment, enhancing uptake, minimizing response. This review explores innovative integration nanocarrier focusing recent advancements, applications, future directions in therapeutic editing.

Language: Английский

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Engineered Exosomes as Smart Drug Carriers: Overcoming Biological Barriers in CNS and Cancer Therapy

Drugs and Drug Candidates, Journal Year: 2025, Volume and Issue: 4(2), P. 19 - 19

Published: April 24, 2025

Engineered exosomes have emerged as transformative drug carriers, uniquely equipped to overcome biological barriers in central nervous system (CNS) disorders and cancer therapy. These natural extracellular vesicles, derived from cell membranes, offer inherent biocompatibility, low immunogenicity, the ability traverse physiological obstacles such blood–brain barrier (BBB) dense tumor stroma. Recent advances exosome engineering—including surface modification (e.g., ligand conjugation for receptor-mediated targeting) cargo loading (siRNA, CRISPR-Cas systems, chemotherapeutics)—have enhanced their precision therapeutic utility. For CNS delivery, functionalized with brain-homing peptides RVG or TfR ligands) enabled efficient transport of neuroprotective agents gene-editing tools treat Alzheimer’s disease glioblastoma. In oncology, engineered loaded tumor-suppressive miRNAs immune checkpoint inhibitors exploit microenvironment (TME) features, acidity enzyme overexpression, spatially controlled release. Furthermore, hybrid exosome–liposome systems exosome–biomaterial composites are being explored improve payload capacity stability. Despite progress, challenges persist scalable production, batch consistency, regulatory standardization. This review critically evaluates engineering strategies, preclinical success, translational hurdles while proposing innovations AI-driven design patient-derived platforms personalized By bridging nanotechnology biomedicine, can represent a paradigm shift targeted offering safer more effective solutions historically intractable diseases.

Language: Английский

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Research Advances of Engineered Exosomes as Drug Delivery Carrier

ACS Omega, Journal Year: 2023, Volume and Issue: 8(46), P. 43374 - 43387

Published: Nov. 9, 2023

Exosomes are nanoscale vesicles secreted by living cells that have similar membrane composition to parental and carry a variety of proteins, lipids, nucleic acids. Therefore, exosomes certain biological activities play an important role in intercellular communication. On the basis its potential as carrier for drug delivery systems, been engineered compensate shortage natural through various engineering strategies improving efficiency, enhancing targeting tissues organs, extending circulating half-life exosomes. This review focuses on loading drugs different strategies, discussions exosome surface modification summarizes advantages disadvantages strategies. In addition, this provides overview recent applications number refractory relapsable diseases. has provide reference further research development

Language: Английский

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