Epigenetic Regulation and Molecular Mechanisms of Burn Injury-Induced Nociception in the Spinal Cord of Mice

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8510 - 8510

Published: Aug. 4, 2024

Epigenetic mechanisms, including histone post-translational modifications (PTMs), play a critical role in regulating pain perception and the pathophysiology of burn injury. However, epigenetic regulation molecular mechanisms underlying injury-induced remain insufficiently explored. Spinal dynorphinergic (Pdyn) neurons contribute to heat hyperalgesia induced by severe scalding-type injury through p-S10H3-dependent signaling. Beyond p-S10H3, may impact various other H3 PTMs. Double immunofluorescent staining protein analyses demonstrated significant hypermethylation at H3K4me1 H3K4me3 sites hyperphosphorylation S10H3 within spinal cord. By analyzing Pdyn dorsal horn, we found evidence chromatin activation with elevation p-S10H3 immunoreactivity. We used RNA-seq analysis compare effects formalin-induced inflammatory on cord transcriptomic profiles. identified 98 DEGs for 86 pain. A limited number shared differentially expressed genes (DEGs) suggest distinct central processing between formalin models. KEGG pathway supported this divergence, activating Wnt This study enhances our understanding uncovers converging diverging pathways models different origins.

Language: Английский

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Effects of Sophora alopecuroides in a High-Concentrate Diet on the Liver Immunity and Antioxidant Function of Lambs According to Transcriptome Analysis

Animals, Journal Year: 2024, Volume and Issue: 14(2), P. 182 - 182

Published: Jan. 5, 2024

The purpose of this study was to determine the effects Sophora alopecuroides (SA) on liver function, inflammatory factor levels, antioxidant indexes and transcriptome in sheep. Twenty-four 3-month-old healthy Dumont hybrid lambs weighing 25.73 ± 2.17 kg were randomly divided into three groups: C1 (the control group), fed a concentrate-to-forage ratio 50:50; H2 high-concentration 70:30; S3 SA 70:30 + 0.1% SA. results showed that rumen pH values groups significant or significantly higher than group (p < 0.05 p 0.01). serum ALT, AST LDH activities LPS LBP concentrations sheep extremely those 0.01), IL-10 content SOD, GPX-PX T-AOC opposite trend KEGG enrichment analysis differentially expressed genes enriched ECM–receptor interaction focal adhesion pathways, which are closely related immune functions (p-adjust 0.1). In summary, could improve lamb livers under high-concentrate conditions regulate mechanism damage livers, is caused by diets through expression ECM/FAs pathway.

Language: Английский

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Group XIV C-type lectins: emerging targets in tumor angiogenesis

Angiogenesis, Journal Year: 2024, Volume and Issue: 27(2), P. 173 - 192

Published: March 12, 2024

Abstract C-type lectins, distinguished by a lectin binding domain (CTLD), are an evolutionarily conserved superfamily of glycoproteins that implicated in broad range physiologic processes. The group XIV subfamily CTLDs comprised CD93, CD248/endosialin, CLEC14a, and thrombomodulin/CD141, have important roles creating maintaining blood vessels, organizing extracellular matrix, balancing pro- anti-coagulative As such, dysregulation the expression downstream signaling pathways these proteins often lead to clinically relevant pathology. Recently, been shown play significant cancer progression, namely tumor angiogenesis metastatic dissemination. Interest therapeutically targeting vasculature is increasing search for novel angiogenic targets ongoing. Group emerged as key moderators metastasis, thus offering substantial therapeutic promise clinic. Herein, we review our current knowledge CTLDs, discuss each’s role malignancy associated potential avenues, briefly context two other families, offer future direction further elucidating mechanisms which function facilitate growth.

Language: Английский

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Advancements in Immunotherapeutic Treatments for Hepatocellular Carcinoma: Potential of Combination Therapies

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6830 - 6830

Published: June 21, 2024

Hepatocellular carcinoma (HCC) is the sixth most prevalent cancer and a significant global health burden, with increasing incidence rates limited treatment options. Immunotherapy has become promising approach due to its ability affect immune microenvironment promote antitumor responses. The performs an essential role in both progression development of HCC, different characteristics based on specific cells etiological factors. Immune checkpoint inhibitors, including programmed death-1/programmed death-ligand 1 inhibitors (pembrolizumab, nivolumab, durvalumab) cytotoxic T lymphocyte antigen-4 (tremelimumab ipilimumab), have potential treat advanced HCC overcome adverse effects, such as liver failure chemoresistance. Phase II phase III clinical trials highlight efficacy pembrolizumab respectively, patients, demonstrated by their positive effects overall survival progression-free survival. Tremelimumab exhibited modest response rates, though it does possess antiviral activity. Thus, still being investigated ongoing trials. Combination therapies multiple drugs benefits terms tumor improving patient outcomes compared monotherapy, especially for advanced-stage HCC. This review addresses immunotherapies early-, intermediate-, Additionally, highlights how combination therapy can significantly enhance survival, objective rate where options are limited.

Language: Английский

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Association of the Single Nucleotide Polymorphisms rs11556218, rs4778889, rs4072111, and rs1131445 of the Interleukin-16 Gene with Ovarian Cancer

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10272 - 10272

Published: Sept. 24, 2024

Single nucleotide polymorphisms (SNPs) of the IL-16 gene have been reported to influence risk several cancers, but their role in ovarian cancer (OC) has not studied. Using restriction fragment length polymorphism (PCR-RFLP) method, we examined four SNPs: rs11556218 (T > G), rs4778889 C), rs4072111 (C T), and rs1131445 C) blood samples from 413 women Central European descent, including 200 OC patients 213 healthy controls. Among patients, 62% were postmenopausal, 84.5% diagnosed late stages (FIGO IIb-IV), 73.5% had high-grade serous (HGSOC). Minor allele frequencies controls 9.2% for (G allele), 13.7% 10.4% 32.3% allele). We found significant associations vs. T allele: OR 2.76, 95% CI 1.84–4.14, p < 0.0001) with elevated whole cohort (p 0.001) both premenopausal postmenopausal = subgroups. These remained across heterozygote 0.001), dominant overdominant models. was associated predominantly 0.0001 almost all models). In cohort, C (OR 1.54, 1.06–2.23, 0.024), association 0.019), 0.033), 0.027) Furthermore, linked HGSOC 0.036) endometriosis-related subtypes 0.002). No or 0.81 0.47, respectively). conclusion, SNPs are risk, especially women.

Language: Английский

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