Journal of Applied Crystallography, Journal Year: 2024, Volume and Issue: 57(5), P. 1514 - 1527
Published: Sept. 25, 2024
Energy-dispersive Laue diffraction (EDLD) is a powerful method to obtain position-resolved texture information in inhomogeneous biological samples without the need for sample rotation. This study employs EDLD scanning investigate impact of two salivary peptides, statherin (STN) and histatin-1 (HTN) 21
Language: Английский
Evolutionary Anthropology Issues News and Reviews, Journal Year: 2025, Volume and Issue: 34(1)
Published: March 1, 2025
Unlike developmental biologists, paleoanthropologists primarily investigate development using skeletal remains, specifically fossilized and already-formed bones teeth. Focusing on peri- and/or postnatal growth, they reconstruct from fragmented "snapshots" of individual trajectories at various ontogenetic stages. These constraints prompt a discussion what defines versus its boundaries in studies hominin evolution. We explore how address the limitations fossil record by diverse methodological theoretical frameworks to identify markers despite missing data. Finally, we discuss potential "Extended Evolutionary Synthesis," which calls for greater focus processes interpreting phenotypic variation record.
Language: Английский
Citations
0
Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
The paper proposes a strategy for the accelerated deposition of biomimetic organomineral layers on surface dental enamel, utilizing di/tetrahydroquinolinediol (hydroxyquinoline) polymerized in presence nanocrystalline hydroxyapatite (nano-cHAp). mechanisms underlying formation coatings were elucidated through combination structural, microstructural, and spectroscopic analytical methods, including synchrotron infrared nanoimaging. Additionally, antimicrobial effects these investigated. It has been demonstrated that an layer, based dihydroxyquinoline, natural enamel leads to agglomeration orientation nanocrystals within coating. This process enables layer replicate mechanical properties resulting microhardness value closely resembles enamel. Using s-SNOM, it established possesses morphological structure poly(2,2,4-trimethyl-1,2-dihydroquinoline-6,7-diol (TMDHQ))/nano-cHAp composite film, which is homogeneously distributed tightly packed surface. Furthermore, coating formed from polydihydroxyquinoline exhibits inhibitory activity against colonies Streptococcus spp. developed technology layers, exhibit simultaneous antibacterial mineralizing effects, holds significant potential future clinical applications.
Language: Английский
Citations
0
Dentistry Journal, Journal Year: 2023, Volume and Issue: 11(5), P. 130 - 130
Published: May 9, 2023
Caries is a chronic disease that causes the alteration of structure dental tissues by acid dissolution (in enamel, dentine and cementum) proteolytic degradation (dentine generates an important cost care. There need to visualise characterise process on enamel due its hierarchical leading complex structural modifications. The starts at surface progresses into depth, which necessitates study internal structure. Artificial demineralisation usually employed simulate experimentally. In present study, human was studied using analysis carried out with atomic force microscopy as well 3D synchrotron X-ray tomography during exposure repeated scans generate time-lapse visualisation sequence. Two-dimensional from projections virtual slices mass provided details tissue changes level rods inter-rod substance. addition modifications, rate determined, demonstrated feasibility usefulness these techniques. temporal not limited can be applied other experimental conditions for treated or remineralisation.
Language: Английский
Citations
9
Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 181, P. 263 - 271
Published: April 25, 2024
Language: Английский
Citations
3
Materials & Design, Journal Year: 2023, Volume and Issue: 234, P. 112369 - 112369
Published: Sept. 27, 2023
Exceptional mechanical performance of dental enamel in the harsh environment oral cavity can be preserved on decade timescale, which is a unique property comparison with functional nanomaterials. Hierarchic architecture enamel, based site-specific structural organization apatite nanocrystals has key role this durability. In present study, novel SEM imaging method presented for obtaining quantitative information prism orientation sound primary enamel. This missing puzzle quantification hierarchical structure, along spatial and chemical mapping, shed light optimum anisotropic gradient behaviour elastic modulus Specifically, composition dependent contributions both spatially changing hardness were separated. Anisotropy enamel's was predicted verified by variation average orientation. Based our results we conclude that anisotropy bulk arises from direction normal to external surface combined nearly constant value perpendicular cross section. high strength additionally responsible superior durability human
Language: Английский
Citations
8
Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2(3), P. 222 - 232
Published: March 8, 2024
The Dual Imaging and Diffraction (DIAD) beamline at Diamond Light Source (Didcot, U.K.) implements a correlative approach to the dynamic study of materials based on concurrent analysis identical sample locations using complementary X-ray modalities reveal structural detail various length scales. Namely, underlying principle its practical implementation allow collocation chosen regions within their interrogation real-space imaging (radiography tomography) reciprocal space scattering (diffraction). switching between two principal modes is made smooth rapid by design, so that data collected interlaced obtain near-simultaneous multimodal characterization. Different specific photon energies are used for each mode, interlacing acquisition steps allows conducting static experiments. Building demonstrated realization this state-of-the-art requires further refining experimental practice, namely, methods gauge volume under different beam–sample interaction. To address challenge, experiments were conducted DIAD devoted human dental enamel, hierarchical structure composed hydroxyapatite mineral nanocrystals, as previously affected caries (tooth decay) well conditions simulating process acid demineralization. Collocation correlation achieved WAXS (wide-angle scattering), 2D (radiographic), 3D (tomographic) imaging. While in or reveals details microstructure, provided statistical nanoscale ultrastructural polycrystal reciprocal-space information such phase preferred orientation (texture). Careful registration positions recorded during scans allowed direct covisualization from modalities. volumes identified visualized tomographic sets, revealing local support interpretation diffraction patterns. present 4D microscopy paradigm following progression demineralization with time-dependent pattern evolution an transferable other material systems.
Language: Английский
Citations
2
ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(31), P. 37259 - 37273
Published: July 31, 2023
Caries, a major global disease associated with dental enamel demineralization, remains insufficiently understood to devise effective prevention or minimally invasive treatment. Understanding the ultrastructural changes in is hampered by lack of nanoscale characterization chemical spatial distributions within tissue. This leads requirement develop techniques based on various methods. The purpose present study demonstrate strength analytic methods using correlative technique single sample human as specific case test accuracy compare regions enamel. science different integrated genuinely hierarchical structures carious tissue were mapped combination focused ion beam scanning electron microscopy synchrotron X-ray tomography. studied fluorescence (XRF) and wide-angle small-angle scattering size below 80 nm for ångström nanometer length scales. analysis XRF intensity gradients revealed subtle variations Ca samples comparison those normal mature In addition, pathways rod demineralization ptychography. results show structural modification differing locations. These reinforce need multi-modal approaches complex hierarchically structured materials interpret materials. approach establishes meticulous platform biomineralized tissues at nanoscale, which adds confidence interpretation time-saving imaging techniques. protocol demonstrated here can be applied other statistical investigation changes. information gathered from could not obtained traditional individual
Language: Английский
Citations
5
Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2(3), P. 213 - 221
Published: Feb. 8, 2024
High-resolution spatial and temporal analysis 3D visualization of time-dependent processes, such as human dental enamel acid demineralization, often present a challenging task. Overcoming this challenge requires the development special methods. Dental caries remains one most important oral diseases that involves demineralization hard tissues consequence production by bacteria. Enamel has hierarchically organized architecture extends down to nanostructural level high resolution study its evolution in detail. is dynamic process best investigated with help situ experiments. In previous studies, synchrotron tomography was applied structure at certain time points (time-lapse tomography). Here, another distinct approach time-evolving studies presented, whereby sample image reconstructed it undergoes continuous rotation over virtually unlimited angular range. The resulting (single) data set contains for multiple (potentially overlapping) intermediate tomograms can be extracted analyzed desired using time-stepping selection subsets from fly-scan recording. One advantages reduces amount required collect an equivalent number single tomograms. Another advantage nominal step between successive reconstructions significantly reduced. We acidic observed progression steps smaller than total acquisition tomogram, voxel size 0.5 μm. It expected presented paper useful high-resolution other processes assessing small structural modifications evolving hierarchical materials.
Language: Английский
Citations
1
Nano-Structures & Nano-Objects, Journal Year: 2024, Volume and Issue: 38, P. 101205 - 101205
Published: May 1, 2024
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11297 - 11297
Published: Oct. 21, 2024
This study primarily focused on the acid erosion of enamel and dentin. A detailed examination X-ray diffraction data proves that products acid-caused decay belong to family isomorphic bioapatites, especially calcium-deficient hydroxyapatites. They are a trajectory towards less crystallized substances. The increase in Bragg’s parameter d decrease energy necessary for changes were coupled with variability pH. was valid corrosive action solutions pH greater than 3.5. When processes natural tooth aging studied by diffraction, clear similarity teeth revealed. Scarce osteoporotic bones seemed confirm conclusions derived teeth. concerning bioapatite decays confronted cycles apatite synthesis/decay. chemical studies, mainly Ca/P ratio relation range durability popular compounds engaged synthesis/decay apatites, suggested process formation under influence acids much inverted starting from brushite up apatite, an alkaline environment. Our simulations showed shift between bioapatites versus apatites reaction model stoichiometric equations associated particular stages relevant derived. synthesis alkalization reactions dehydration. hydrolysis reactions. Formally, environment during is presented introducing Ca(OH)2 equations.
Language: Английский
Citations
1