COX-2 Inhibition by Bioactive Peptides from Peanut Worm (Siphonosoma australe) Collagen Through In Vitro Digestion Simulation DOI Creative Commons

Suwarjoyowirayatno Suwarjoyowirayatno,

Chusnul Hidayat, Tutik Dwi Wahyuningsih

et al.

Food Technology and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Research background. Chronic, unregulated inflammation is a crucial factor in developing numerous diseases and closely linked to the increased expression of cyclooxygenase-2 (COX-2). While various bioactive peptides from marine organisms have exhibited COX-2 inhibitory effects, derived collagen peanut worm (Siphonosoma australe) not yet been demonstrated. This study aimed evaluate collagen's potential activity through vitro simulated digestion using pepsin-pancreatin followed by molecular docking. Experimental approach. During digestion, commercial pepsin (at pH 3) pancreatin 7.5) were applied for 240 min at 37 °C degree hydrolysis, peptide concentration, activity. Samples showing most significant subsequently separated into fractions identified. Results conclusions. The 210 showed highest (64.31 %). finding was confirmed elevated hydrolysis (DH) concentrations observed during digestion. fraction <1 kDa (89.05 %), sequencing. Three novel peptides, ADIAGQAAQVLR, LNNEITTLR, VGTVEK, identified contain amino acids, confirming them as inhibitors. VGTVEK has potent interaction, shown lowest binding energy (−4.41 kcal/mol). docking revealed that (631.35 Da) binds active side COX-2, forming hydrogen bonds with Gln178, Leu338, Ser339, Tyr371, Ile503, Phe504, Val509, Ser516 hydrophobic interactions Met99, Val102, Val330, Ile331, Tyr334, Val335, Leu345, Trp373, Leu517, Leu520. Other biological activities produced included ACE inhibitors, DPP-IV α-glucosidase According toxicity prediction, classified non-toxic. Novelty scientific contribution. found generated exhibit novel, natural agents anti-inflammatory therapy. Their broader application functional foods, nutraceuticals, pharmaceuticals could offer new options individual sufferers inflammation, supporting both treatment overall health maintenance.

Language: Английский

Bioactive compounds for human and planetary health DOI Creative Commons
Martin Kussmann,

David Henrique Abe Cunha,

Silvia Berciano

et al.

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: July 17, 2023

Bioactive compounds found in edible plants and foods are vital for human planetary health, yet their significance remains underappreciated. These natural bioactives, as part of whole diets, ingredients, or supplements, can modulate multiple aspects health wellness. Recent advancements omic sciences computational biology, combined with the development Precision Nutrition, have contributed to convergence nutrition medicine, well more efficient affordable healthcare solutions that harness power food prevention therapy. Innovation this field is crucial feed a growing global population sustainably healthily. This requires significant changes our system, spanning agriculture, production, distribution consumption. As we facing pressing challenges, investing bioactive-based an opportunity protect biodiversity soils, waters, atmosphere, while also creating value consumers, patients, communities, stakeholders. Such research innovation targets include alternative proteins, such cellular agriculture plant-derived protein; extracts improve shelf-life preservatives; upcycling agricultural by-products reduce waste; alternatives synthetic fertilizers pesticides. Translational bioactives currently being developed at two levels, using systems-oriented approach. First, biological level, interplay between these host microbiome elucidated through omics research, big data artificial intelligence, accelerate both discovery validation. Second, ecosystem efforts focused on producing diverse nutrient-rich, flavorful, resilient, high-yield crops, educating consumers make informed choices benefit planet. Adopting system-oriented perspective helps: unravel intricate dynamic relationships nutrition, sustainability outcomes, harnessing nature promote wellbeing; foster sustainable ecosystem. Interdisciplinary collaboration needed new era practical food-based some most challenges humanity planet today.

Language: Английский

Citations

48
Purification, identification and molecular docking studies of antioxidant and anti-inflammatory peptides from Edible Bird's Nest DOI
Chia Hau Lee, Norfadilah Hamdan, Bemgba Bevan Nyakuma

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 454, P. 139797 - 139797

Published: May 22, 2024

Language: Английский

Citations

25
Alternation in sequence features and their influence on the anti-inflammatory activity of soy peptides during digestion and absorption in different enzymatic hydrolysis conditions DOI
Wanlu Liu, Yu Han, Jiulong An

et al.

Food Chemistry, Journal Year: 2025, Volume and Issue: 471, P. 142824 - 142824

Published: Jan. 8, 2025

Language: Английский

Citations

4
Antioxidant and Anti-Inflammatory Effect of Cinnamon (Cinnamomum verum J. Presl) Bark Extract after In Vitro Digestion Simulation DOI Creative Commons
Stefania Pagliari, Matilde Forcella, Elena Lonati

et al.

Foods, Journal Year: 2023, Volume and Issue: 12(3), P. 452 - 452

Published: Jan. 18, 2023

Cinnamon bark is widely used for its organoleptic features in the food context and growing evidence supports beneficial effect on human health. The market offers an increasingly wide range of products supplements enriched with cinnamon extracts which are eliciting health-promoting properties. Specifically, extract Cinnamomum spp. rich antioxidant, anti-inflammatory anticancer biomolecules. These include reported cinnamic acid some phenolic compounds, such asproanthocyanidins A B, kaempferol. molecules sensitive to physical-chemical properties (such as pH temperature) biological agents that act during gastric digestion, could impair molecules’ bioactivity. Therefore, this study, cinnamon’s antioxidant bioactivity after simulated digestion was evaluated by analyzing chemical profile pure digested one, well cellular vitro models, Caco2 intestinal barrier. results showed digestive process reduces total content polyphenols, especially tannins, while preserving other bioactive compounds acid. At functional level, maintains at level.

Language: Английский

Citations

31
An insight into role of amino acids as antioxidants via NRF2 activation DOI Creative Commons
Melford C. Egbujor, Olugbemi T. Olaniyan, Chigbundu N. Emeruwa

et al.

Amino Acids, Journal Year: 2024, Volume and Issue: 56(1)

Published: March 20, 2024

Oxidative stress can affect the protein, lipids, and DNA of cells thus, play a crucial role in several pathophysiological conditions. It has already been established that oxidative close association with inflammation via nuclear factor erythroid 2-related 2 (NRF2) signaling pathway. Amino acids are notably building block proteins constitute major class nitrogen-containing natural products medicinal importance. They exhibit broad spectrum biological activities, including ability to activate NRF2, transcription regulates endogenous antioxidant responses. Moreover, amino may act as synergistic antioxidants part our dietary supplementations. This aroused research interest NRF2-inducing activity acids. Interestingly, acids' activation NRF2-Kelch-like ECH-associated protein 1 (KEAP1) pathway exerts therapeutic effects diseases. Therefore, present review will discuss relationship between different NRF2-KEAP1 pinning their anti-inflammatory properties. We also discussed formulations applications therapeutics. broaden prospect myriad stress-related provide an insight for designing developing new chemical entities NRF2 activators.

Language: Английский

Citations

14
Food Peptides for the Nutricosmetic Industry DOI Creative Commons
Irene Dini, Andrea Mancusi

Antioxidants, Journal Year: 2023, Volume and Issue: 12(4), P. 788 - 788

Published: March 23, 2023

In recent years, numerous reports have described bioactive peptides (biopeptides)/hydrolysates produced from various food sources. Biopeptides are considered interesting for industrial application since they show functional properties (e.g., anti-aging, antioxidant, anti-inflammatory, and antimicrobial properties) technological solubility, emulsifying, foaming). Moreover, fewer side effects than synthetic drugs. Nevertheless, some challenges must be overcome before their administration via the oral route. The gastric, pancreatic, small intestinal enzymes acidic stomach conditions can affect bioavailability levels that reach site of action. Some delivery systems been studied to avoid these problems microemulsions, liposomes, solid lipid particles). This paper summarizes results studies conducted on biopeptides isolated plants, marine organisms, animals, biowaste by-products, discusses potential in nutricosmetic industry, considers could maintain bioactivity. Our environmentally sustainable products used as antimicrobial, anti-inflammatory agents formulations. Biopeptide production requires expertise analytical procedures good manufacturing practice. It is hoped new developed simplify large-scale authorities adopt regulate use appropriate testing standards guarantee population’s safety.

Language: Английский

Citations

17
Alterations in the sequence and bioactivity of food-derived oligopeptides during simulated gastrointestinal digestion and absorption: a review DOI
Wanlu Liu, Kexin Li,

Shengjuan Yu

et al.

International Journal of Food Sciences and Nutrition, Journal Year: 2024, Volume and Issue: 75(2), P. 134 - 147

Published: Jan. 7, 2024

Food-derived oligopeptides (FOPs) exhibit various bioactivities. However, little was known about their sequence changes in the gastrointestinal tract and effect of on FOPs' features, effects bioactivities have been summarised. The length FOPs decreases with increased exposure hydrophobic basic amino acids at terminal during simulated digestion. A decrease after intestinal absorption has correlated a Leu, Ile, Arg, Tyr, Gln Pro. that pass readily through epithelium corresponds to transport modes, whose sequences remain unchanged are most bioactive. These include mainly dipeptides tetrapeptides, consisting numerous acids, found mostly end peptide chain, especially C-terminal. This review aims provide foundation for applications nutritional supplements functional foods.

Language: Английский

Citations

6
A quinoa peptide protects impaired mucus barriers in colitis mice by inhibiting NF-κB-TRPV1 signaling and regulating the gut microbiota DOI
Luanfeng Wang,

Shufeng Wu,

Tong Chen

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(3), P. 1223 - 1236

Published: Jan. 1, 2024

Inflammatory bowel diseases (IBD) are chronic inflammatory conditions that lead to the disruption of colonic mucus barrier. Quinoa has a well-balanced profile essential amino acids and exhibits excellent anti-inflammatory effects. We recently explored beneficial effects relevant mechanisms novel quinoa peptide TPGAFF on impaired barriers in mice with chemically induced colitis. Our findings demonstrated TPGAFF, administered low high doses for 28 days, effectively attenuated pathological phenotype reduced intestinal permeability colitis mice. its protective abilities by restoring barrier, inhibiting activation signaling reducing cytokine levels. Moreover, positively influenced composition gut microbiota inflammation-related microbes. Additionally, inhibited TRPV1 nociceptor decreased levels neuropeptides. Conclusively, our results indicated oral administration may be an optional approach treatment barrier damage.

Language: Английский

Citations

5
A comparative study of the digestion behavior and functionality of protein from chia (Salvia hispanica L.) ingredients and protein fractions DOI Creative Commons
Yan Wang, Alan Javier Hernández‐Álvarez, Francisco M. Goycoolea

et al.

Current Research in Food Science, Journal Year: 2024, Volume and Issue: 8, P. 100684 - 100684

Published: Jan. 1, 2024

Protein derived from chia (

Language: Английский

Citations

5
Macromolecules with predominant β-pleated sheet proteins in extracellular vesicles released from Raphanus sativus L. var. caudatus Alef microgreens induce DNA damage-mediated apoptosis in HCT116 colon cancer cells DOI

Karnchanok Kaimuangpak,

Reny Rosalina, Kanjana Thumanu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 269, P. 132001 - 132001

Published: May 1, 2024

Language: Английский

Citations

5