Structural characteristics of neutral polysaccharides purified from coix seed and its anti‐insulin resistance effects on HepG2 cells DOI Creative Commons

Guozhen Wu,

Shuang Liu,

Zhenqiang Wang

et al.

Food Science & Nutrition, Journal Year: 2024, Volume and Issue: 12(10), P. 8419 - 8431

Published: Sept. 8, 2024

Abstract Coix seed is recognized as a functional medicinal food due to its valuable biological activities, with polysaccharides being the primary active compounds. In this study, an ultrasonic‐assisted enzymatic extraction technique was employed, and response surface methodology used optimize yield of 9.55 ± 0.26%. A novel neutral polysaccharide, CSPsN‐1, purified molecular weight 7.75 kDa. CSPsN‐1 composed arabinose, galactose, glucose, xylose, mannose in molar ratios 0.48: 7.92: 86.39: 2.42: 2.79. Its backbone →4)‐α‐D‐Glc p ‐(1→ →3,4)‐α‐D‐Glc units, terminal residues α‐D‐Glc . vitro experiments, enhanced glucose consumption insulin‐resistant HepG2 cells upregulated GLUT4 expression by activating PI3K/AKT signaling pathway. These findings suggest that holds significant promise ingredient for treating insulin resistance related metabolic disorders.

Language: Английский

Analysis and properties of polysaccharides extracted from Brassica oleracea L. Var. Capitata L. By hot water extraction/ultrasonic-synergistic enzymatic method DOI Creative Commons
Huilin Wang, Gangliang Huang,

Xixin Zhang

et al.

Ultrasonics Sonochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 107244 - 107244

Published: Jan. 1, 2025

Language: Английский

Citations

5
Structural Characterizations and Biological Evaluation of a Natural Polysaccharide from Branches of Camellia oleifera Abel DOI Creative Commons

Shengjia Lu,

Yali Zhang,

Yanghui Ou

et al.

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(1), P. 51 - 51

Published: Jan. 3, 2025

Background: Camellia oleifera Abel (C. oleifera) is widely cultivated and serves as an important source of edible oil. Yet, during oil production, pruned branches generate significant waste contribute to environmental pollution. Objectives: In this work, we obtain a natural polysaccharide from the C. optimize its extraction using Box–Behnken design (BBD), which statistical method commonly used in response surface methodology. Additionally, study properties, such monosaccharide composition, structural features, antioxidant, anti-inflammatory abilities. Results: BBD was employed (solid-liquid ratio = 1:40; 90 °C; 130 min) for higher yield. After purification, major monosaccharides oleifera’s (CBP) were disclosed glucose galactose. Subsequent features CBP measured. The antioxidant abilities highly scavenging rates 2,2-diphenyl-1-picrylhydrazyl hydroxyl radicals, with chelating capacity Fe2+, indicate potent activity CBP. Conclusions: general, demonstrated down-regulating expression IL-6 IL-1β LPS-induced macrophage RAW264.7 model. This bioactive adds value by offering novel approach recycling development oleifera.

Language: Английский

Citations

2
Polysaccharides from sea buckthorn — Ultrasound-assisted enzymatic extraction, purification, structural characterization, and antioxidant activity analysis DOI Creative Commons
Wen Jing, Ruijie Huang,

Shi Li

et al.

Food Chemistry X, Journal Year: 2025, Volume and Issue: 26, P. 102265 - 102265

Published: Feb. 1, 2025

This study employed a sophisticated approach consisting of ultrasound-assisted enzyme treatment to extract polysaccharides from sea buckthorn (SBP). The SBP extraction parameters were optimized, the following optimal identified: solid-liquid ratio 1:32 g/mL, ultrasound duration 26 min, temperature 52 °C, and composite concentration 6000 U/100 mL, maximum yield was 24.07 ± 0.15 %. separation purification resulted in isolation three fractions (SBPR-1, SBPR-2, SBPR-3). composition structural characteristics SBPRs identified. Furthermore, exhibited characteristic absorption peaks polysaccharides. Notably, surface microstructures showed significant variations. Moreover, all demonstrated commendable thermal stability vitro antioxidant activity. serves as reference for development application natural antioxidants provides theoretical foundation environmentally friendly effective SBP.

Language: Английский

Citations

2
Ultrasound-assisted enzymatic extraction of soluble dietary fiber from Hericium erinaceus and its in vitro lipid-lowering effect DOI Creative Commons

Panling Yu,

Xueyu Pan, Mingjie Chen

et al.

Food Chemistry X, Journal Year: 2024, Volume and Issue: 23, P. 101657 - 101657

Published: July 14, 2024

Dietary fiber (DF) is an important active polysaccharide in

Language: Английский

Citations

14
Ultrasound-assisted enzymatic extraction, process optimization, and antioxidant activity of polysaccharides from sugarcane peel DOI Creative Commons
Bo Mao, Wei Lu, Gangliang Huang

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 11, 2025

In this research, ultrasound-assisted enzymatic extraction of sugarcane peel polysaccharides and optimization process conditions were used to investigate their properties antioxidant activities. The effects liquid-material ratio, ultrasonic temperature, time, cellulase dosage on the rate SPP investigated by one-way experiments response surface analysis determine optimal conditions. Meanwhile, structural characterization (FT-IR NMR analyses) activity studies (DPPH hydroxyl radical scavenging rates) carried out total sugar content SPP. results showed that for method 40 mL/g 50 °C ultrasound min 5% enzyme dosage, yield was 5.38% 75.52% under these extracted some ability both DPPH radicals, 54.63% 33.91%, respectively, when mass concentration solution 4 mg/mL. present study demonstrated enzymes can effectively extract with activity, which provides a theoretical basis developing utilizing has potential application value.

Language: Английский

Citations

1
Separation, Purification, Structural Characterization, and In Vitro Hypoglycemic Activity of Polysaccharides from Panax notoginseng Leaves DOI Creative Commons

X.-Q. Zhang,

Chongying Shi,

Zilin Wang

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 830 - 830

Published: Feb. 11, 2025

This study optimized the extraction process of crude polysaccharides from Panax notoginseng leaves (PNLP) using ultrasonic-assisted dual-enzyme method through a single-factor combined with response surface experiment. The were subsequently purified and isolated DEAE-Cellulose 52, followed by structural analysis, evaluation antioxidant activity, examination digestive enzyme inhibition. hypoglycemic effects components further clarified. results indicated that polysaccharide had an yield 17.13 ± 0.29%. fraction PNLP-3 (eluted 0.3 M NaCl) was obtained 52 chromatography, exhibiting total sugar content 81.2% molecular weight 16.57 kDa. PNLP is primarily composed arabinose, galactose, galacturonic acid, molar percentages 20.24%, 33.54%, 24.27%, respectively. mainly arabinose 29.97% 49.35%, In this IC50 values for α-glucosidase α-amylase inhibition 1.045 mg/mL 9.53 mg/mL, Molecular docking confirmed exhibits better inhibitory activity against α-glucosidase. Furthermore, alleviated hyperglycemia in insulin-resistant HepG2 cells enhancing glucose consumption glycogen synthesis. exhibited positive correlation its concentration, potentially contributing to reducing oxidative stress. These findings underscore therapeutic potential leaf managing type 2 diabetes offer new perspectives on use natural regulating blood glucose.

Language: Английский

Citations

1
Current developments and trends in hybrid extraction techniques for green analytical applications in natural products DOI

N. K. Soni,

Mukul Yadav,

M. Malarvannan

et al.

Journal of Chromatography B, Journal Year: 2025, Volume and Issue: 1256, P. 124543 - 124543

Published: Feb. 27, 2025

Language: Английский

Citations

1
Recent advances in polysaccharide-based drug delivery systems for cancer therapy: a comprehensive review DOI Creative Commons
Chou‐Yi Hsu, Omer Qutaiba B. Allela,

Ali M. Hussein

et al.

Artificial Cells Nanomedicine and Biotechnology, Journal Year: 2024, Volume and Issue: 52(1), P. 564 - 586

Published: Dec. 5, 2024

Cancer has a high rate of incidence and mortality throughout the world. Although several conventional approaches have been developed for treatment cancer, such as surgery, chemotherapy, radiotherapy thermal therapy, they remarkable disadvantages which result in inefficient cancer. For example, immunogenicity, prolonged treatment, non-specificity, metastasis cost are considered major drawbacks chemotherapy. Therefore, there is fundamental requirement development breakthrough technologies cancer suppression. Polysaccharide-based drug delivery systems (DDSs) most reliable carriers therapy. Polysaccharides, kind practical biomaterials, divided into types, including chitosan, alginates, dextran, hyaluronic acid, cyclodextrin, pectin, etc. Polysaccharides extracted from different natural resources (like herbal, marine, microorganisms, etc.). The potential features polysaccharides made them candidates therapeutics to sites; simple purification, ease modification functionalization, hydrophilicity, serum stability, appropriate loading capacity, biocompatibility, bioavailability, biodegradability stimuli-responsive sustained release manner considerable aspects these biopolymers. This review highlights applications polysaccharides-based DDSs pharmaceutical science

Language: Английский

Citations

7
Isolation, structure, biological activity and application progress of ginseng polysaccharides from the Araliaceae family DOI
Silu Liu,

Jianan Geng,

Weijia Chen

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133925 - 133925

Published: July 18, 2024

Language: Английский

Citations

5
Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides DOI Creative Commons

Mengdie Ren,

Yiru Hou,

Danli Peng

et al.

Ultrasonics Sonochemistry, Journal Year: 2025, Volume and Issue: 114, P. 107259 - 107259

Published: Feb. 7, 2025

This study investigates two extraction methods-ultrasound-assisted (UAE) and compound enzyme (CE)-for the isolation of Lonicera macranthoides polysaccharides (LMP) evaluates their physicochemical properties, bioactivity, therapeutic potential. The optimization conditions was performed using single-factor experiments response surface methodology. yield LMP from UAE optimized to 8.02 % ± 0.12 %, while CE 10.03 0.32 %. Both polysaccharide extracts were characterized in terms chemical composition, monosaccharide molecular weight, thermal stability, functional group analysis. Notably, UAE-LMP exhibited superior antioxidant, hypolipidemic, nitrite scavenging, anti-tumor activities compared CE-LMP. ultrasound-assisted method enhanced stability bioactivity LMP, with improved water- oil-holding capacities integrity active groups. Our findings demonstrate that significantly influences structural properties suggesting may be a more effective approach for extracting bioactive potential applications medicine nutrition.

Language: Английский

Citations

0