Food Science & Nutrition,
Journal Year:
2024,
Volume and Issue:
12(10), P. 8419 - 8431
Published: Sept. 8, 2024
Abstract
Coix
seed
is
recognized
as
a
functional
medicinal
food
due
to
its
valuable
biological
activities,
with
polysaccharides
being
the
primary
active
compounds.
In
this
study,
an
ultrasonic‐assisted
enzymatic
extraction
technique
was
employed,
and
response
surface
methodology
used
optimize
yield
of
9.55
±
0.26%.
A
novel
neutral
polysaccharide,
CSPsN‐1,
purified
molecular
weight
7.75
kDa.
CSPsN‐1
composed
arabinose,
galactose,
glucose,
xylose,
mannose
in
molar
ratios
0.48:
7.92:
86.39:
2.42:
2.79.
Its
backbone
→4)‐α‐D‐Glc
p
‐(1→
→3,4)‐α‐D‐Glc
units,
terminal
residues
α‐D‐Glc
.
vitro
experiments,
enhanced
glucose
consumption
insulin‐resistant
HepG2
cells
upregulated
GLUT4
expression
by
activating
PI3K/AKT
signaling
pathway.
These
findings
suggest
that
holds
significant
promise
ingredient
for
treating
insulin
resistance
related
metabolic
disorders.
Pharmaceuticals,
Journal Year:
2025,
Volume and Issue:
18(1), P. 51 - 51
Published: Jan. 3, 2025
Background:
Camellia
oleifera
Abel
(C.
oleifera)
is
widely
cultivated
and
serves
as
an
important
source
of
edible
oil.
Yet,
during
oil
production,
pruned
branches
generate
significant
waste
contribute
to
environmental
pollution.
Objectives:
In
this
work,
we
obtain
a
natural
polysaccharide
from
the
C.
optimize
its
extraction
using
Box–Behnken
design
(BBD),
which
statistical
method
commonly
used
in
response
surface
methodology.
Additionally,
study
properties,
such
monosaccharide
composition,
structural
features,
antioxidant,
anti-inflammatory
abilities.
Results:
BBD
was
employed
(solid-liquid
ratio
=
1:40;
90
°C;
130
min)
for
higher
yield.
After
purification,
major
monosaccharides
oleifera’s
(CBP)
were
disclosed
glucose
galactose.
Subsequent
features
CBP
measured.
The
antioxidant
abilities
highly
scavenging
rates
2,2-diphenyl-1-picrylhydrazyl
hydroxyl
radicals,
with
chelating
capacity
Fe2+,
indicate
potent
activity
CBP.
Conclusions:
general,
demonstrated
down-regulating
expression
IL-6
IL-1β
LPS-induced
macrophage
RAW264.7
model.
This
bioactive
adds
value
by
offering
novel
approach
recycling
development
oleifera.
Food Chemistry X,
Journal Year:
2025,
Volume and Issue:
26, P. 102265 - 102265
Published: Feb. 1, 2025
This
study
employed
a
sophisticated
approach
consisting
of
ultrasound-assisted
enzyme
treatment
to
extract
polysaccharides
from
sea
buckthorn
(SBP).
The
SBP
extraction
parameters
were
optimized,
the
following
optimal
identified:
solid-liquid
ratio
1:32
g/mL,
ultrasound
duration
26
min,
temperature
52
°C,
and
composite
concentration
6000
U/100
mL,
maximum
yield
was
24.07
±
0.15
%.
separation
purification
resulted
in
isolation
three
fractions
(SBPR-1,
SBPR-2,
SBPR-3).
composition
structural
characteristics
SBPRs
identified.
Furthermore,
exhibited
characteristic
absorption
peaks
polysaccharides.
Notably,
surface
microstructures
showed
significant
variations.
Moreover,
all
demonstrated
commendable
thermal
stability
vitro
antioxidant
activity.
serves
as
reference
for
development
application
natural
antioxidants
provides
theoretical
foundation
environmentally
friendly
effective
SBP.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Feb. 11, 2025
In
this
research,
ultrasound-assisted
enzymatic
extraction
of
sugarcane
peel
polysaccharides
and
optimization
process
conditions
were
used
to
investigate
their
properties
antioxidant
activities.
The
effects
liquid-material
ratio,
ultrasonic
temperature,
time,
cellulase
dosage
on
the
rate
SPP
investigated
by
one-way
experiments
response
surface
analysis
determine
optimal
conditions.
Meanwhile,
structural
characterization
(FT-IR
NMR
analyses)
activity
studies
(DPPH
hydroxyl
radical
scavenging
rates)
carried
out
total
sugar
content
SPP.
results
showed
that
for
method
40
mL/g
50
°C
ultrasound
min
5%
enzyme
dosage,
yield
was
5.38%
75.52%
under
these
extracted
some
ability
both
DPPH
radicals,
54.63%
33.91%,
respectively,
when
mass
concentration
solution
4
mg/mL.
present
study
demonstrated
enzymes
can
effectively
extract
with
activity,
which
provides
a
theoretical
basis
developing
utilizing
has
potential
application
value.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(4), P. 830 - 830
Published: Feb. 11, 2025
This
study
optimized
the
extraction
process
of
crude
polysaccharides
from
Panax
notoginseng
leaves
(PNLP)
using
ultrasonic-assisted
dual-enzyme
method
through
a
single-factor
combined
with
response
surface
experiment.
The
were
subsequently
purified
and
isolated
DEAE-Cellulose
52,
followed
by
structural
analysis,
evaluation
antioxidant
activity,
examination
digestive
enzyme
inhibition.
hypoglycemic
effects
components
further
clarified.
results
indicated
that
polysaccharide
had
an
yield
17.13
±
0.29%.
fraction
PNLP-3
(eluted
0.3
M
NaCl)
was
obtained
52
chromatography,
exhibiting
total
sugar
content
81.2%
molecular
weight
16.57
kDa.
PNLP
is
primarily
composed
arabinose,
galactose,
galacturonic
acid,
molar
percentages
20.24%,
33.54%,
24.27%,
respectively.
mainly
arabinose
29.97%
49.35%,
In
this
IC50
values
for
α-glucosidase
α-amylase
inhibition
1.045
mg/mL
9.53
mg/mL,
Molecular
docking
confirmed
exhibits
better
inhibitory
activity
against
α-glucosidase.
Furthermore,
alleviated
hyperglycemia
in
insulin-resistant
HepG2
cells
enhancing
glucose
consumption
glycogen
synthesis.
exhibited
positive
correlation
its
concentration,
potentially
contributing
to
reducing
oxidative
stress.
These
findings
underscore
therapeutic
potential
leaf
managing
type
2
diabetes
offer
new
perspectives
on
use
natural
regulating
blood
glucose.
Artificial Cells Nanomedicine and Biotechnology,
Journal Year:
2024,
Volume and Issue:
52(1), P. 564 - 586
Published: Dec. 5, 2024
Cancer
has
a
high
rate
of
incidence
and
mortality
throughout
the
world.
Although
several
conventional
approaches
have
been
developed
for
treatment
cancer,
such
as
surgery,
chemotherapy,
radiotherapy
thermal
therapy,
they
remarkable
disadvantages
which
result
in
inefficient
cancer.
For
example,
immunogenicity,
prolonged
treatment,
non-specificity,
metastasis
cost
are
considered
major
drawbacks
chemotherapy.
Therefore,
there
is
fundamental
requirement
development
breakthrough
technologies
cancer
suppression.
Polysaccharide-based
drug
delivery
systems
(DDSs)
most
reliable
carriers
therapy.
Polysaccharides,
kind
practical
biomaterials,
divided
into
types,
including
chitosan,
alginates,
dextran,
hyaluronic
acid,
cyclodextrin,
pectin,
etc.
Polysaccharides
extracted
from
different
natural
resources
(like
herbal,
marine,
microorganisms,
etc.).
The
potential
features
polysaccharides
made
them
candidates
therapeutics
to
sites;
simple
purification,
ease
modification
functionalization,
hydrophilicity,
serum
stability,
appropriate
loading
capacity,
biocompatibility,
bioavailability,
biodegradability
stimuli-responsive
sustained
release
manner
considerable
aspects
these
biopolymers.
This
review
highlights
applications
polysaccharides-based
DDSs
pharmaceutical
science
Ultrasonics Sonochemistry,
Journal Year:
2025,
Volume and Issue:
114, P. 107259 - 107259
Published: Feb. 7, 2025
This
study
investigates
two
extraction
methods-ultrasound-assisted
(UAE)
and
compound
enzyme
(CE)-for
the
isolation
of
Lonicera
macranthoides
polysaccharides
(LMP)
evaluates
their
physicochemical
properties,
bioactivity,
therapeutic
potential.
The
optimization
conditions
was
performed
using
single-factor
experiments
response
surface
methodology.
yield
LMP
from
UAE
optimized
to
8.02
%
±
0.12
%,
while
CE
10.03
0.32
%.
Both
polysaccharide
extracts
were
characterized
in
terms
chemical
composition,
monosaccharide
molecular
weight,
thermal
stability,
functional
group
analysis.
Notably,
UAE-LMP
exhibited
superior
antioxidant,
hypolipidemic,
nitrite
scavenging,
anti-tumor
activities
compared
CE-LMP.
ultrasound-assisted
method
enhanced
stability
bioactivity
LMP,
with
improved
water-
oil-holding
capacities
integrity
active
groups.
Our
findings
demonstrate
that
significantly
influences
structural
properties
suggesting
may
be
a
more
effective
approach
for
extracting
bioactive
potential
applications
medicine
nutrition.