Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 4, 2024
To
prevent
the
spread
of
foodborne
illnesses,
presence
pathogens
in
food
chain
is
monitored
by
government
agencies
and
producers.
The
culture-based
methods
currently
employed
are
sensitive
but
time-
labor-intensive,
leading
to
increasing
interest
exploring
culture-independent
diagnostic
tests
(CIDTs)
for
pathogen
detection.
However,
few
studies
quantify
relative
sensitivity
reliability
these
CIDTs
compared
current
approaches.
address
this
issue,
we
conducted
a
comparison
limit
detection
(LOD
Drug Design Development and Therapy,
Journal Year:
2025,
Volume and Issue:
Volume 19, P. 585 - 598
Published: Jan. 1, 2025
The
beneficial
effects
of
probiotics
are
encountered
by
their
low
viability
in
gastrointestinal
conditions
and
insufficient
stability
during
manufacturing,
throughut
the
transit,
storage.
Therefore,
novel
systems
highly
required
to
improve
delivery.
In
this
study,
Lactobacillus
gasseri
(L),
Bifidobacterium
bifidum
(B),
a
combination
L+B
were
encapsulated
chitosan
(CS)-polyacrylic
acid
(PAA)
complex
(CS-PAA).
CS-PAA
analysed
on
basis
morphology,
size,
zeta
potential.
loaded
evaluated
for
particle
zetapotential,
vaiability
both
simulated
gastroic
intestinal
fluids,
at
4°C
storage
temparature.
Moreover,
antibiofilm
activity
probiotics-loaded
againt
Campylobacter
jejuni,
Pseudomonas
aeruginosa
Escherichia
coli.
Probiotic
strains
successfully
incorporated
into
porous
structures
systems,
either
individually
or
combination.
Loaded
L,
B,
showed
higher
size
than
unloaded
particles
excellent
gastric
where
free
probiotic
species
undetected.
Additionally
exhibited
an
anti-biofilm
effect
0.5
mg/mL
concentration
level.
complexes
demonstrate
promising
mechanism
effective
delivery
probiotics.
high
maintain
under
physiological
conditions,
remarkable
activity.
These
characteristics
suggest
that
could
serve
as
alternative
system
probiotics,
enhancing
gut
microbiota
health
offering
robust
protection
against
microbial
pathogens.
Antibiotics,
Journal Year:
2023,
Volume and Issue:
12(8), P. 1281 - 1281
Published: Aug. 3, 2023
Probiotics
are
among
those
products,
the
use
of
which
is
increasing,
and
they
available
primarily
as
food/dietary
supplements,
well
in
form
medicines.
This
study
aims
to
assess
attitudes
practices
health
professionals
students
sciences,
give
a
short
overview
probiotics
currently
on
market,
conduct
screening
five
food
supplements
one
drug
with
respect
antibiotic
resistance.
Nearly
half
respondents
our
survey
state
that
have
no
side
effects,
while
only
6.3%
believe
can
lead
In
addition,
more
than
40%
participants
throw
unused
into
municipal
waste.
The
market
analysis
results
indicate
probiotic
products
Serbian
highly
variable
CFU
counts,
declared
claims
cover
numerous
beneficial
sometimes
even
registered
Lactobacilli
frequently
present
sold
pharmacies
online.
experimental
showed
resistance
different
types
lactobacilli
products.
risk
using
probiotics,
regardless
their
should
be
taken
account
future.
An
update
regulations
governing
including
stipulation
for
antimicrobial
(AMR)
testing,
established,
guidelines
proper
disposal
put
place.
Foods,
Journal Year:
2024,
Volume and Issue:
13(10), P. 1538 - 1538
Published: May 15, 2024
This
review
examines
the
techno-functional
properties
of
lactic
acid
bacteria
(LABs)
in
food
industry,
focusing
on
their
potential
health
benefits.
We
discuss
current
findings
related
to
techno-functionality
LAB,
which
includes
acidification,
proteolytic
and
lipolytic
features,
a
variety
other
biochemical
activities.
These
activities
include
production
antimicrobial
compounds
synthesis
exopolysaccharides
that
improve
safety
consumer
sensory
experience.
LABs
are
also
known
for
antioxidant
abilities,
help
reduce
oxidative
reactions
foods
functional
properties.
In
addition,
LABs’
role
as
probiotics
is
promising
effects
gut
health,
immune
system
modulation,
cholesterol
control,
general
wellbeing.
Despite
these
advantages,
several
challenges
hinder
effective
use
probiotic
LABs,
such
maintaining
strain
viability
during
storage
transport
well
ensuring
efficacy
gastrointestinal
tract.
Our
identifies
critical
barriers
suggests
avenues
future
research.
Microorganisms,
Journal Year:
2022,
Volume and Issue:
11(1), P. 95 - 95
Published: Dec. 30, 2022
This
review
aims
to
bring
a
more
general
view
of
the
technological
and
biological
challenges
regarding
production
use
probiotic
bacteria
in
promoting
human
health.
After
brief
description
current
concepts,
for
at
an
industrial
level
are
presented
from
physiology
central
metabolism
ability
face
main
forms
stress
process.
Once
produced,
these
cells
processed
be
commercialized
suspension
or
dried
added
food
matrices.
At
this
stage,
maintenance
cell
viability
vitality
is
paramount
quality
product.
Powder
products
requires
development
strategies
that
ensure
integrity
components
cellular
functions
allow
complete
recovery
time
consumption.
Finally,
once
consumed,
must
very
powerful
set
physicochemical
mechanisms
within
body,
which
include
enzymes,
antibacterial
molecules
sudden
changes
pH.
Understanding
action
agents
induction
tolerance
fundamental
selection
increasingly
efficient
strains
order
survive
colonization
intestinal
tract
promote
desired
health
benefits.
International Journal of Pharmaceutics,
Journal Year:
2023,
Volume and Issue:
641, P. 123058 - 123058
Published: May 18, 2023
3D
Printing
is
an
innovative
technology
within
the
pharma
and
food
industries
that
allows
design
manufacturing
of
novel
delivery
systems.
Orally
safe
probiotics
to
gastrointestinal
tract
faces
several
challenges
regarding
bacterial
viability,
in
addition
comply
with
commercial
regulatory
standpoints.
Lactobacillus
rhamnosus
CNCM
I-4036
(Lr)
was
microencapsulated
generally
recognised
as
(GRAS)
proteins,
then
assessed
for
robocasting
printing.
Microparticles
(MP-Lr)
were
developed
characterised,
prior
being
printed
pharmaceutical
excipients.
MP-Lr
showed
a
size
12.3
±
4.1
µm
non-uniform
wrinkled
surface
determined
by
Scanning
Electron
Microscopy
(SEM).
Bacterial
quantification
plate
counting
accounted
8.68
0.6
CFU/g
live
bacteria
encapsulated
within.
Formulations
able
keep
dose
constant
upon
contact
gastric
intestinal
pH.
Printlets
consisted
oval-shape
formulations
(15
mm
×
8
3.2
mm)
ca.
370
mg
total
weight,
uniform
surface.
After
printing
process,
viability
remained
even
protected
alongside
process
(log
reduction
0.52,
p
>
0.05)
comparison
non-encapsulated
probiotic
3.05).
Moreover,
microparticle
not
altered
during
process.
We
confirmed
success
this
developing
orally
formulation,
GRAS
category,
Lr
vehiculation.
Cytometry Part A,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 3, 2025
ABSTRACT
Flow
cytometry
(FC)
is
a
versatile
and
powerful
tool
in
microbiology,
enabling
precise
analysis
of
single
cells
for
variety
applications,
including
the
detection
quantification
bacteria,
viruses,
fungi,
as
well
algae,
phytoplankton,
parasites.
Its
utility
assessing
cell
viability,
metabolic
activity,
immune
responses,
pathogen‐host
interactions
makes
it
indispensable
both
research
diagnostics.
The
microbiota
(community
microorganisms)
microbiome
(collective
genomes
has
become
essential
understanding
intricate
role
microbial
communities
health,
disease,
physiological
functions.
FC
offers
promising
complement,
providing
rapid,
cost‐effective,
dynamic
profiling
communities,
with
added
ability
to
isolate
sort
bacterial
populations
further
analysis.
In
probiotic
industry,
facilitates
fast,
affordable,
analyses,
helping
assess
probiotics
postbiotics.
It
also
supports
study
viability
under
stress
conditions,
gastric
acid
bile,
improving
insight
into
survival
adhesion
intestinal
mucosa.
Additionally,
integration
Machine
Learning
microbiology
transformative
potential,
data
supporting
advances
personalized
medicine
formulations.
Despite
need
standardization,
continues
evolve
key
modern
clinical
Frontiers in Antibiotics,
Journal Year:
2025,
Volume and Issue:
4
Published: May 6, 2025
The
worldwide
increasing
frequency
and
severity
of
multidrug-resistant
gastrointestinal
(MDR-GI)
infections
not
only
raises
awareness
the
debilities
conventional
antibiotic
treatments
but
also
highlights
demand
for
alternative
interventions.
One
these
alternatives
is
probiotics,
harmless
bacteria
that
compete
with
pathogenic
species,
which
have
been
considered
beneficial
due
to
their
therapeutic
potential
since
they
strengthen
mucosal
barrier
modulate
host
immune
response.
Other
natural
compounds
(e.g.,
polyphenols,
flavonoids,
essential
oils)
present
diverse
antimicrobial
mechanisms,
are
promising
mitigate
resistant
pathogens.
Finally,
bacteriophages,
viruses
target
specific
bacteria,
constitute
a
precise
approach
in
MDR
lysed
or
disrupted
by
biofilms
formed
during
colonization
without
compromising
normal
gut
microbiome.
Therefore,
manuscript
provides
an
integrated
perspective
on
non-antibiotic
therapies
manage
MDR-GI
infections;
this
purpose,
it
covers
aspects
such
as
action
current
clinical
applications,
challenges
limit
broader
application
practice.
combining
approaches
personalizing
infection
adjusted
patients’
microbiome
profiles
discussed,
aiming
enhance
efficacy
reduce
resistance
risks.
importance
continued
research
development
optimize
debated,
addressing
need
surpass
regulatory
barriers
conducting
large-scale
trials
establish
safety
alternatives.
This
overview
knowledge
contributes
ongoing
efforts
develop
sustainable
strategies
combat
global
burden
resistance.
