Polymers,
Journal Year:
2021,
Volume and Issue:
13(3), P. 366 - 366
Published: Jan. 24, 2021
Coronavirus
disease
2019
(COVID-19),
which
has
recently
emerged
as
a
global
pandemic,
caused
serious
economic
crisis
due
to
the
social
disconnection
and
physical
distancing
in
human
society.
To
rapidly
respond
emergence
of
new
diseases,
reliable
vitro
model
needs
be
established
expeditiously
for
identification
appropriate
therapeutic
agents.
Such
models
can
great
help
validating
pathological
behavior
pathogens
Recently,
representing
organs
tissues
biological
functions
have
been
developed
based
on
high-precision
3D
bioprinting.
In
this
paper,
we
delineate
an
in-depth
assessment
bioprinting
technology
bioinks.
particular,
discuss
latest
achievements
future
aspects
use
modeling.
Biofabrication,
Journal Year:
2019,
Volume and Issue:
11(4), P. 042001 - 042001
Published: July 17, 2019
The
dawn
of
3D
printing
in
medicine
has
given
the
field
hope
vitality
many
patients
fighting
a
multitude
diseases.
Also
entitled
bioprinting,
this
appertains
to
its
sequential
precursor
ink,
embodying
cells
and
polymer/composite
predetermined
trajectory.
addition
cells,
is
predominantly
constituted
hydrogels
due
biodegradability
ability
mimic
body's
anatomy
mechanical
features,
e.g.
bones,
etc.
This
review
paper
devoted
explicating
bioprinting
(3D/4D)
alginate
hydrogels,
which
are
extracts
from
brown
algae,
through
extrusion
additive
manufacturing.
Alginates
salt
derivatives
alginic
acid
constitute
long
chains
polysaccharides,
provides
pliability
gelling
adeptness
their
structure.
Alginate
hydrogel
(employed
for
extrusion)
can
be
pristine
or
composite
relying
on
requisite
properties
(target
application
controlled
vivo
environment),
alginate-natural
(gelatin/agarose/collagen/hyaluronic
acid/etc)
alginate-synthetic
(polyethylene
glycol
(PEG)/pluronic
F-127/etc).
Extrusion
manufacturing
preponderate
among
others
with
uncomplicated
processing,
material
efficiency
(cut
down
wastage),
outspread
adaptability
viscosities
(0.03–6
*
104
Pa.s),
but
procedure
limited
by
resolution
(200
μm)
accuracy.
However,
3D-fabricated
biostructures
display
rigidness
(unvarying
conditions)
i.e.
lacks
smart
response,
reassured
accounting
time
feature
as
noteworthy
accessory
printing,
interpreted
4D
bioprinting.
propounds
specific
processing
itinerary
(meanwhile
traversing
across
composites/blends
natural
synthetic
consideration)
along
pre-/during/post-processing
parameters
intrinsic
process.
Furthermore,
propensity
also
presented
(alginate
processing)
tissue
engineering,
cartilage
(joints),
brain
(neural),
ear,
heart
(cardiac),
eyes
(corneal),
etc,
worldwide
quandary
over
accessibility
organs
diverse
types
Additionally,
contemplates
recently
invented
advance
biotic
species,
challenges
future
opportunities.
Chemical Reviews,
Journal Year:
2020,
Volume and Issue:
120(19), P. 10662 - 10694
Published: April 17, 2020
This
review
provides
a
detailed
overview
of
the
rapidly
advancing
field
biofabrication,
particularly
with
regards
to
use
photo-cross-linking
(i.e.,
light-based)
techniques.
The
major
emphasis
this
is
on
fundamentals
and
key
criteria
identified
for
successful
design
implementation
photo-cross-linked
bioinks
bioresins
in
extrusion-based
lithography-based
bioprinting.
general
mechanisms
associated
(e.g.,
free-radical
chain
polymerization,
thiol–ene,
photomediated
redox)
natural
synthetic
materials
are
described
inform
bioink
bioresin
design,
which
includes
selection
polymers,
functional
group
modifications,
photoinitiators,
light
sources
that
enable
facile
cytocompatible
photo-cross-linking.
Depending
material
bioprinting
technique
interest,
we
describe
specific
or
properties
must
be
achieved
ensure
optimal
printability
utility.
Finally,
examples
current
state-of-the-art
applications
light-based
vitro
tissue
models,
engineering,
regenerative
medicine
provided
further
motivate
future
opportunities
within
landscape
facilitated
light.
International Journal of Molecular Sciences,
Journal Year:
2018,
Volume and Issue:
19(12), P. 4117 - 4117
Published: Dec. 18, 2018
Tissue
engineering
and
regenerative
medicine
involve
many
different
artificial
biologic
materials,
frequently
integrated
in
composite
scaffolds,
which
can
be
repopulated
with
various
cell
types.
One
of
the
most
promising
scaffolds
is
decellularized
allogeneic
extracellular
matrix
(ECM)
then
recellularized
by
autologous
or
stem
cells,
order
to
develop
fully
personalized
clinical
approaches.
Decellularization
protocols
have
efficiently
remove
immunogenic
cellular
maintaining
nonimmunogenic
ECM,
endowed
specific
inductive/differentiating
actions
due
its
architecture
bioactive
factors.
In
present
paper,
we
review
available
literature
about
development
grafts
from
human
tissues/organs.
Human
tissues
may
obtained
not
only
surgery
but
also
cadavers,
suggesting
possible
BioBanks
body
donation
programs.
Many
tissues/organs
been
for
tissue
purposes,
such
as
cartilage,
bone,
skeletal
muscle,
tendons,
adipose
tissue,
heart,
vessels,
lung,
dental
pulp,
intestine,
liver,
pancreas,
kidney,
gonads,
uterus,
childbirth
products,
cornea,
peripheral
nerves.
vitro
recellularizations
reported
types
procedures
(seeding,
injection,
perfusion).
Conversely,
studies
vivo
behaviour
are
poorly
represented.
Actually,
future
challenge
will
implanted
restored
all
their
structural/functional
aspects.
Materials,
Journal Year:
2021,
Volume and Issue:
14(4), P. 858 - 858
Published: Feb. 10, 2021
Sustaining
the
vital
functions
of
cells
outside
organism
requires
strictly
defined
parameters.
In
order
to
ensure
their
optimal
growth
and
development,
it
is
necessary
provide
a
range
nutrients
regulators.
Hydrogels
are
excellent
materials
for
3D
in
vitro
cell
cultures.
Their
ability
retain
large
amounts
liquid,
as
well
biocompatibility,
soft
structures,
mechanical
properties
similar
these
living
tissues,
appropriate
microenvironments
that
mimic
extracellular
matrix
functions.
The
wide
natural
synthetic
polymeric
materials,
simplicity
physico-chemical
modification,
allow
be
adjusted
different
requirements.
Sodium
alginate-based
hydrogel
frequently
used
material
culture.
lack
cell-interactive
makes
this
polysaccharide
most
often
applied
combination
with
other
including
gelatin.
both
increases
biological
activity
improves
properties,
making
printing
technology.
use
hydrogels
inks
allows
accurate
manufacturing
scaffolds
complex
shapes
geometries.
aim
paper
an
overview
cultures,
which
mainly
alginate–gelatin
hydrogels,
potential
applications.
Bioengineering,
Journal Year:
2021,
Volume and Issue:
8(2), P. 27 - 27
Published: Feb. 20, 2021
The
most
prevalent
form
of
bioprinting—extrusion
bioprinting—can
generate
structures
from
a
diverse
range
materials
and
viscosities.
It
can
create
personalized
tissues
that
aid
in
drug
testing
cancer
research
when
used
combination
with
natural
bioinks.
This
paper
reviews
bioinks
their
properties
functions
hard
soft
tissue
engineering
applications.
discusses
agarose,
alginate,
cellulose,
chitosan,
collagen,
decellularized
extracellular
matrix,
dextran,
fibrin,
gelatin,
gellan
gum,
hyaluronic
acid,
Matrigel,
silk.
Multi-component
are
considered
as
way
to
address
the
shortfalls
individual
biomaterials.
mechanical,
rheological,
cross-linking
along
cytocompatibility,
cell
viability,
printability
detailed
well.
Future
avenues
for
into
then
presented.
Gut,
Journal Year:
2020,
Volume and Issue:
70(3), P. 567 - 574
Published: May 20, 2020
Objective
Shortage
of
organ
donors,
a
critical
challenge
for
treatment
end-stage
failure,
has
motivated
the
development
alternative
strategies
to
generate
organs
in
vitro.
Here,
we
aim
describe
hepatorganoids,
which
is
liver
tissue
model
generated
by
three-dimensional
(3D)
bioprinting
HepaRG
cells
and
investigate
its
functions
vitro
vivo.
Design
3D
bioprinted
hepatorganoids
(3DP-HOs)
were
constructed
using
bioink,
according
specific
printing
procedures.
Liver
3DP-HOs
detected
after
7
days
differentiation
vitro,
later
transplanted
into
Fah-deficient
mice.
The
vivo
evaluated
survival
time
damage
mice,
human
function
markers
human-specific
debrisoquine
metabolite
production.
Results
broadly
acquired
functions,
such
as
ALBUMIN
secretion,
drug
metabolism
glycogen
storage
differentiation.
After
transplantation
abdominal
cavity
Fah
-/-
Rag2
mouse
injury,
further
matured
displayed
increased
synthesis
liver-specific
proteins.
Particularly,
mice
activities.
Functional
vascular
systems
also
formed
3DP-HOs,
enhancing
material
transport
3DP-HOs.
Most
importantly,
significantly
improved
Conclusions
Our
results
demonstrated
comprehensive
proof
principle,
indicated
that
3DP-HO
tissues
possessed
hepatic
alleviated
failure
transplantation,
suggesting
could
be
used
donors
diseases.
