Antibacterial activity and characteristics of silver nanoparticles biosynthesized from Carduus crispus

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Oct. 26, 2021

Abstract In recent years’ synthesis of metal nanoparticle using plants has been extensively studied and recognized as a non-toxic efficient method applicable in biomedical field. The aim this study is to investigate the role different parts medical plant Carduus crispus on synthesizing silver nanoparticles characterize produced nanoparticle. Our showed that (AgNP) synthesized via whole extract exhibited blue shift absorption spectra with increased optical density, which correlates high yield small size. Also, results zeta potential, X-ray diffraction, photon cross-correlation spectroscopy analysis surface charge − 54.29 ± 4.96 mV (AgNP-S), 42.64 3.762 (AgNP-F), 46.02 4.17 (AgNP-W), crystallite size 36 nm 13 14 (AgNP-W) face-centered cubic structure average grain sizes 145.1 nm, 22.5 99.6 nm. Another important characteristic, such elemental composition constituent capping agent determined by energy-dispersive Fourier transform infrared. were composed ~ 80% Ag, 15% K, 7.5% Ca (or 2.8% P) elements. Moreover, FTIR measurement suggested distinct functional groups present both AgNP-S AgNP-F found AgNP-W. atomic force microscopy revealed AgNP-S, AgNP-W had 131 33 70 respectively. addition, biosynthesized evaluated for their cytotoxicity antibacterial activity. At 17 µg/ml concentration, very low toxicity HepG2 cell line but also activity gram-negative bacterium Escherichia coli (5.5 0.2 mm 6.5 0.3 mm) gram-positive Micrococcus luteus (7 0.4 7.7 0.5 mm). meaningful first observation indicating possibility special organs control characteristics nanoparticles.

Language: Английский

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Sanitizing agents for virus inactivation and disinfection

View, Journal Year: 2020, Volume and Issue: 1(2)

Published: May 24, 2020

Viral epidemics develop from the emergence of new variants infectious viruses. The lack effective antiviral treatments for viral infections coupled with rapid community spread infection often result in major human and financial loss. transmissions can occur via close human-to-human contact or contacting a contaminated surface. Thus, careful disinfection sanitization is essential to curtail spread. A myriad disinfectants/sanitizing agents/biocidal agents are available that inactivate viruses, but their effectiveness dependent upon many factors such as concentration agent, reaction time, temperature, organic load. In this work, we review common commercially disinfectants on market evaluate under various application conditions. addition, work also seeks debunk myths about inactivation highlight exciting advances development potential sanitizing agents.

Language: Английский

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Effect of Size, Shape and Surface Functionalization on the Antibacterial Activity of Silver Nanoparticles

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(5), P. 244 - 244

Published: April 26, 2023

Silver nanoparticles (AgNPs) are the most investigated antibacterial agents against multidrug resistant (MDR) pathogens. They can lead to cellular death by means of different mechanisms, damaging several cell compartments, from external membrane, enzymes, DNA and proteins; this simultaneous attack amplifies toxic effect on bacteria with respect traditional antibiotics. The effectiveness AgNPs MDR is strongly correlated their chemical morphological properties, which influence pathways involved in damage. In review, AgNPs’ size, shape modification functional groups or other materials reported, both investigate synthetic nanoparticles’ modifications evaluate related activity. Indeed, understanding conditions for obtaining performing could help tailor new improved silver-based combat resistance.

Language: Английский

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Kinetics of Silver Accumulation in Tissues of Laboratory Mice after Long-Term Oral Administration of Silver Nanoparticles

Nanomaterials, Journal Year: 2021, Volume and Issue: 11(12), P. 3204 - 3204

Published: Nov. 26, 2021

Since ancient times, silver has been known for its pronounced bactericidal, antiviral and fungicidal properties. Currently, nanoparticles of this metal are widely used in the food, light pharmaceutical industries, as well medicine. Silver any form can have a toxic effect not only on pathogens, but also healthy cells. The biological activity bioavailability preparations depend degree their solubility water. In addition, maximum permissible concentration soluble forms is an order magnitude lower than that insoluble forms. This makes with hydrophilic coating stable colloidal solutions aqueous media potentially unsafe objects. work, we studied kinetics accumulation average size 34 ± 5 nm stabilized polyvinylpyrrolidone organs laboratory C57Bl/6 mice. administration was carried out orally 30, 60, 120 180 days at dose 50 µg/day/animal. All mice developed gained weight normally during experiment. No adverse effects were observed. Determination content tissues mammals accomplished by neutron activation analysis. masses concentrations brain different sections (hippocampus, cerebellum, cortex remnants), lungs, testes, liver, blood, kidneys, spleen heart, determined. injection times which curves reached saturation established. An extremely high testes shown administration, significant lungs all parts except significant, trend similar to whole brain.

Language: Английский

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Biopolymers-Based Materials Containing Silver Nanoparticles as Active Packaging for Food Applications–A Review

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(3), P. 698 - 698

Published: Jan. 21, 2020

Packaging is an integral part of food products, allowing the preservation their quality. It plays important role, protecting packed product from external conditions, maintaining quality, and improving properties packaged during storage. Nevertheless, commonly used packaging based on synthetic non-biodegradable polymers causes serious environmental pollution. Consequently, numerous recent studies have focused development biodegradable materials biopolymers. In addition, biopolymers may be classified as active materials, since they ability to carry different substances. This review presents latest updates use silver nanoparticles in Silver become interesting component biopolymers, mainly due antimicrobial that allow prolong shelf life products. Furthermore, incorporation into lead with improved physical-mechanical properties.

Language: Английский

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Alginate-chitosan oligosaccharide-ZnO composite hydrogel for accelerating wound healing

Carbohydrate Polymers, Journal Year: 2021, Volume and Issue: 266, P. 118100 - 118100

Published: April 24, 2021

Language: Английский

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Green Synthesis and Potential Antibacterial Applications of Bioactive Silver Nanoparticles: A Review

Polymers, Journal Year: 2022, Volume and Issue: 14(4), P. 742 - 742

Published: Feb. 15, 2022

Green synthesis of silver nanoparticles (AgNPs) using biological resources is the most facile, economical, rapid, and environmentally friendly method that mitigates drawbacks chemical physical methods. Various such as plants their different parts, bacteria, fungi, algae, etc. could be utilized for green bioactive AgNPs. In recent years, several approaches non-toxic, facile AgNPs have been reported. Plant extract contains various biomolecules, including flavonoids, terpenoids, alkaloids, phenolic compounds, vitamins act reducing capping agents during biosynthesis process. Similarly, microorganisms produce primary secondary metabolites play a crucial role synthesis. Biosynthesized gained significant attention from researchers because potential applications in fields biomedical science. The widest application bactericidal activity. Due to emergence multidrug-resistant microorganisms, are exploring therapeutic abilities antibacterial agents. Already, reports suggested biosynthesized exhibited action against numerous human pathogens. Because small size large surface area, ability easily penetrate bacterial cell walls, damage membranes, reactive oxygen species, interfere with DNA replication well protein synthesis, result death. This paper provides an overview green, rapid use AgNPs, highlighting mechanisms.

Language: Английский

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An overview of intelligent freshness indicator packaging for food quality and safety monitoring

Trends in Food Science & Technology, Journal Year: 2021, Volume and Issue: 118, P. 285 - 296

Published: Oct. 19, 2021

Language: Английский

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Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

Nanomaterials, Journal Year: 2020, Volume and Issue: 10(10), P. 2082 - 2082

Published: Oct. 21, 2020

An endophytic strain of Streptomyces antimycoticus L-1 was isolated from healthy medicinal plant leaves Mentha longifolia L. and used for the green synthesis silver nanoparticles (Ag-NPs), through use secreted enzymes proteins. UV-vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), dynamic light scattering (DLS) analyses Ag-NPs were carried out. The XRD, TEM, FT-IR analysis results demonstrated successful biosynthesis crystalline, spherical with a particle size 13-40 nm. Further, stability assessed by detecting surface Plasmon resonance (SPR) at 415 nm one month or measuring NPs charge (-19.2 mV) zeta potential (ζ). green-synthesized exhibited broad-spectrum antibacterial activity different concentrations (6.25-100 ppm) against pathogens Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium clear inhibition zone ranging (9.5 ± 0.4) to (21.7 1.0) mm. Furthermore, displayed high efficacy Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC50) = 5.7 0.2 ppm). With respect in-vitro cytotoxicity analyses, 100 ppm selected as safe dose loading onto cotton fabrics. scanning connected energy-dispersive spectroscopy (SEM-EDX) nano-finished fabrics showed distribution 2% total fabric elements. Moreover, more pathogenic Gram-positive Gram-negative bacteria, even after 10 washing cycles, indicating treated

Language: Английский

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Green Synthesis of Silver Nanoparticles Using Aqueous Citrus limon Zest Extract: Characterization and Evaluation of Their Antioxidant and Antimicrobial Properties

Nanomaterials, Journal Year: 2022, Volume and Issue: 12(12), P. 2013 - 2013

Published: June 10, 2022

The current work concentrated on the green synthesis of silver nanoparticles (AgNPs) through use aqueous Citruslimon zest extract, optimizing different experimental factors required for formation and stability AgNPs. preparation was confirmed by observation color change mixture nitrate, after addition plant from yellow to a reddish-brown colloidal suspension established detecting surface plasmon resonance band at 535.5 nm, utilizing UV-Visible analysis. optimum conditions were found be 1 mM nitrate concentration, 1:9 ratio extract mixture, 4 h incubation period. Fourier transform infrared spectroscopy spectrum indicated that phytochemicals compounds present in Citrus limon had fundamental effect production AgNPs as bio-reducing agent. morphology, size, elemental composition investigated zeta potential (ZP), dynamic light scattering (DLS), SEM, EDX, X-ray diffraction (XRD), transmission electron microscopy (TEM) analysis, which showed crystalline spherical nanoparticles. In addition, antimicrobial antioxidant properties this bioactive nanoparticle also investigated. excellent antibacterial activity against one Gram-negative pathogens bacteria, Escherichia coli, Gram-positive Staphylococcus aureus, well antifungal Candida albicans. obtained results indicate is significant. This can used biomedical pharmacological fields.

Language: Английский

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