Problems of Cryobiology and Cryomedicine,
Journal Year:
2022,
Volume and Issue:
32(1), P. 34 - 43
Published: March 30, 2022
Cryopreservation
of
gametes
is
one
the
important
stages
assisted
reproductive
technologies
(ART).
To
obtain
high
results
using
ART,
it
essential
to
cryopreserve
sperm
in
a
favorable
season,
when
have
ability
fertilize
oocytes.
The
aim
this
research
was
study
seasonal
variability
spermatozoa
cryoresistance
and
characteristics
Saanen
goats.
After
removing
seminal
fluid,
five
mature
animals
were
cryopreserved
solution
10%
glycerol
20%
egg
yolk.
examined
by
embryo
development
rate
on
day
7
vitro
culture.
Reproductive
goats
during
breeding
season
found
be
featured
increased
concentration
motility.
survival
goat
after
cryopreservation
seasonally
dependent,
i.
e.
number
viable
notably
higher
than
non-breeding
one.
frequency
oocyte
fertilization
use
fresh
derived
different
seasons
significantly
group.
embryos
which
developed
blastocyst
stage
lowest
oocytes
with
obtained
season.
It
concluded
that
cryotolerance
are
compared
findings
will
allow
develop
strategy
reproduction
farm
for
food
security
Ukraine.
International Journal of Molecular Sciences,
Journal Year:
2020,
Volume and Issue:
21(8), P. 2781 - 2781
Published: April 16, 2020
Sperm
cryopreservation
represents
a
powerful
tool
for
livestock
breeding.
Several
efforts
have
been
made
to
improve
the
efficiency
of
sperm
in
different
ruminant
species.
However,
significant
amount
still
suffers
considerable
cryodamage,
which
may
affect
quality
and
fertility.
Recently,
use
"omics"
technologies
cryobiology,
especially
proteomics
studies,
has
led
better
understanding
molecular
modifications
induced
by
cryopreservation,
facilitating
identification
freezability
biomarkers
certain
proteins
that
can
be
added
before
enhance
cryosurvival.
This
review
provides
an
updated
overview
mechanisms
involved
are
part
responsible
structural,
functional
fertility
changes
observed
frozen-thawed
sperm.
Moreover,
basis
those
factors
freezing
resilience
species
is
also
discussed
as
well
aspects
novel
strategies
developed
reduce
including
new
cryoprotectants,
antioxidants,
proteins,
nanoparticles
vitrification.
Science Advances,
Journal Year:
2023,
Volume and Issue:
9(4)
Published: Jan. 25, 2023
Spermatozoa
need
to
undergo
an
exocytotic
event
called
the
acrosome
reaction
before
fusing
with
eggs.
Although
calcium
ion
(Ca
2+
)
is
essential
for
reaction,
its
molecular
mechanism
remains
unknown.
Ferlin
a
single
transmembrane
protein
multiple
Ca
-binding
C2
domains,
and
there
are
six
ferlins,
dysferlin
(DYSF),
otoferlin
(OTOF),
myoferlin
(MYOF),
fer-1–like
4
(FER1L4),
FER1L5,
FER1L6,
in
mammals.
Dysf
,
Otof
Myof
knockout
mice
have
been
generated,
each
mouse
line
exhibited
membrane
fusion
disorders
such
as
muscular
dystrophy
deafness
abnormal
myogenesis
.
Here,
by
generating
mutant
of
Fer1l4
Fer1l5
Fer1l6
we
found
that
only
required
male
fertility.
spermatozoa
could
migrate
female
reproductive
tract
reach
eggs,
but
no
took
place.
Even
ionophore
cannot
induce
spermatozoa.
These
results
suggest
FER1L5
missing
link
between
reaction.
Genes,
Journal Year:
2024,
Volume and Issue:
15(3), P. 382 - 382
Published: March 20, 2024
Since
artificial
insemination
is
common
practice
in
pig
breeding,
the
quality
and
persistence
of
semen
are
decisive
for
usability
individual
boars.
In
current
study,
genome-wide
association
analyses
were
performed
to
investigate
genetic
variability
underlying
phenotypic
variations
characteristics.
These
traits
comprise
sperm
morphology
motility
under
different
temporal
thermal
storage
conditions,
addition
standard
parameters.
Two
consecutive
samples
fourth
fifth
ejaculates
from
same
boar
comprehensively
analyzed
a
genotyped
Piétrain
population.
A
total
13
genomic
regions
on
chromosomes
identified
that
contain
single-nucleotide
polymorphisms
significantly
associated
with
these
traits.
Subsequent
analysis
revealed
candidate
genes
described
be
involved
spermatogenesis,
such
as
FOXL3,
GPER1,
PDGFA,
PRKAR1B,
SNRK,
SUN1,
TSPO,
motility,
including
ARRDC4,
CEP78,
DNAAF5,
GPER1.
Some
also
male
fertility
or
infertility
mammals
(e.g.,
GPER1).
The
based
laboriously
determined
valuable
phenotypes
contribute
better
understanding
background
pigs
could
prospectively
improvement
through
breeding
approaches.
Biomolecules,
Journal Year:
2020,
Volume and Issue:
10(7), P. 1046 - 1046
Published: July 14, 2020
To
improve
artificial
insemination
protocols
in
ovine
species
it
is
crucial
to
optimize
sperm
quality
evaluation
after
preservation
technologies.
Emerging
technologies
based
on
novel
biomolecules
and
related
redox
balance
proteins
involved
motility
such
as
ProAKAP4
could
be
successfully
applied
ram
evaluation.
In
this
work,
a
multiparametric
analysis
of
fresh,
cooled,
cryopreserved
was
performed
at
different
complexity
levels.
Samples
were
evaluated
terms
(total
motility,
progressive
curvilinear
velocity),
viability,
apoptosis,
content
reactive
oxygen
species,
oxidation‒reduction
potential,
expression
concentration.
As
expected,
samples
showed
significant
decrease
(p
<
0.05),
evidencing
freezability
classes
among
that
detected
by
analyses.
However,
cooled
no
differences
found
concerning
ROS
content,
compared
fresh
explain
the
reported
fertility
rates.
although
proportion
positive-cells
remained
unaltered
control,
concentration
protein
significantly
decreased
0.05)
samples.
This
altered
level
contribute
rates
some
authors.
More
importantly,
can
established
promising
diagnostic
parameter
allowing
us
conservation
finally
species.
Frontiers in Cell and Developmental Biology,
Journal Year:
2021,
Volume and Issue:
9
Published: July 16, 2021
Sperm
cells
are
of
unique
elongated
structure
and
function,
the
development
which
is
tightly
regulated
by
existing
proteins
posttranslational
modifications
(PTM)
these
proteins.
Based
on
phylogenetic
relationships
various
swine
breeds,
Yorkshire
boar
believed
to
be
distinctly
different
from
Duroc
boar.
The
comprehensive
differential
proteomics
phosphoproteomics
profilings
were
performed
spermatozoa
both
boars.
By
peptide
PTM
quantification
followed
statistical
analyses,
167
differentially
expressed
identified
1,745
proteins,
283
phosphopeptides
corresponding
102
phosphorylated
measured
1,140
derived
363
representative
results
validated
Western
blots.
Pathway
enrichment
analyses
revealed
that
majority
expression
phosphorylation
primarily
concerned
with
spermatogenesis,
male
gamete
generation,
sperm
motility,
energy
metabolism,
cilium
morphogenesis,
axonemal
dynein
complex
assembly,
sperm–egg
recognition,
capacitation.
Remarkably,
assembly
related
such
as
SMCP,
SUN5,
ODF1,
AKAP3,
AKAP4
play
a
key
regulatory
role
in
physiological
functions,
significantly
higher
than
Yorkshire.
Furthermore,
sperm-specific
CABYR,
ROPN1,
CALM1,
PRKAR2A,
PRKAR1A,
participates
regulation
motility
mainly
through
cAMP/PKA
signal
pathway
demonstrating
protein
may
an
important
mechanism
underlying
diversity.
Protein–protein
interaction
analysis
14
overlapped
between
potentially
played
flagellum,
including
AKAP4,
FSIP2,
SUN5.
Taken
together,
physiologically
functionally
(DEPs)
(DPPs)
constitute
proteomic
backgrounds
two
breeds.
validation
will
delineate
roles
modulators
spermatozoa.
Animals,
Journal Year:
2023,
Volume and Issue:
13(4), P. 579 - 579
Published: Feb. 7, 2023
The
semen
quality
is
one
of
the
determinant
factors
ram
cryopreservation.
present
retrospective
study
aimed
to
characterize
seasonal
pattern
during
year
for
ten
Portuguese
local
sheep
breeds,
hypothesizing
that
breed
and
season
had
low
effects
on
main
spermatozoa
traits.
A
total
1471
ejaculates
were
used
evaluated
(fresh
semen)
from
85
rams
between
2004
2020
re-evaluated
after
thawing
(thawed
semen).
effect
breed,
season,
sperm
cryopreservation
nine
traits
evaluated.
volume
per
ejaculate,
(SPZ)
concentration,
number
SPZ
affected
by
(p
<
0.001)
but
not
>
0.05).
As
expected,
processing
was
most
significant
factor
variation
seminal
parameters.
Moreover,
interactions
×
processing,
modulated
response
alive
SPZ,
abnormal
morphology,
head,
intermediate
piece
defects.
In
fresh
semen,
only
defects
due
highest
percentage
observed
February
April
period
in
some
breeds.
Overall,
despite
mentioned
particularities,
there
similarities
among
We
also
concluded
ejaculate
our
region.
These
breeds
have
seasonality
can
be
employed
natural
mating
as
well
donors
assisted
reproductive
biotechnologies
whole
at
latitude.
