Contribution of semen to early embryo development: fertilization and beyond

Human Reproduction Update, Journal Year: 2023, Volume and Issue: 29(4), P. 395 - 433

Published: March 7, 2023

Abstract BACKGROUND It has long been thought that the factors affecting embryo and foetal development were exclusively maternally derived; hence, if issues regarding fertility to arise, blame traditionally placed solely on mother. An escalating interest in how paternal influence development, however, begun prove otherwise. Evidence suggests both seminal plasma (SP) sperm contribute multiple shape embryogenesis. This review thus focuses role semen driving early embryonic describes factors, such as SP, centriole, proteins, RNA, DNA, its integrity, together with epigenetics, may female reproductive tract post-fertilization events. The important contributions of highlight imperative need for further research this area, which is sure bring forth breakthroughs leading improvements infertility diagnosis ART well reducing risk miscarriage. OBJECTIVE AND RATIONALE provides a comprehensive overview human embryo, aim providing better understanding SP divisions, gene protein expression, miscarriage, congenital diseases. SEARCH METHODS PubMed searches performed using terms ‘sperm structure’, ‘capacitation’, ‘acrosome reaction’, ‘fertilization’, ‘oocyte activation’, ‘PLCζ’, ‘PAWP’, ‘sperm-borne oocyte activation factor’, deficiency’, centriole’, transport’, mitochondria’, ‘seminal plasma’, epigenetics’, histone modifications’, DNA methylation’, ‘sperm-derived transcripts’, proteins’, fragmentation’, mRNA’, miRNAs’, piRNAs’, aneuploidy’. reviewed articles restricted those published English between 1980 2022. OUTCOMES data suggest male-derived much more than just male haploid genome embryo. indicates contributes help fate These include from RNA integrity. In addition, epigenetic changes have an impact tract, fertilization, stages development. For example, recent proteomic transcriptomic studies identified several sperm-borne markers play roles fertilization WIDER IMPLICATIONS highlights are required work tandem counterparts allow correct A deeper shuttled over cell can shed light improve andrological perspective. Further aid preventing passing genetic abnormalities origin, decreasing incidence factor infertility. exact mechanisms contribution assist scientists IVF clinicians determining new causes recurrent miscarriage or failure.

Language: Английский

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Sperm Lipid Markers of Male Fertility in Mammals

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(16), P. 8767 - 8767

Published: Aug. 16, 2021

Sperm plasma membrane lipids are essential for the function and integrity of mammalian spermatozoa. Various lipid types involved in each key step within fertilization process their own yet coordinated way. The balance between metabolism is tightly regulated to ensure physiological cellular processes, especially referring crucial steps such as sperm motility, capacitation, acrosome reaction or fusion. At same time, it has been shown that male reproductive depends on homeostasis lipids. Here, we review effects phospholipid, neutral glycolipid fertility mammals.

Language: Английский

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Analysis of 206 whole‐genome resequencing reveals selection signatures associated with breed‐specific traits in Hu sheep

Evolutionary Applications, Journal Year: 2024, Volume and Issue: 17(6)

Published: June 1, 2024

Abstract As an invaluable Chinese sheep germplasm resource, Hu are renowned for their high fertility and beautiful wavy lambskins. Their distinctive characteristics have evolved over time through a combination of artificial natural selection. Identifying selection signatures in can provide straightforward insight into the mechanism further uncover candidate genes associated with breed‐specific traits subject to Here, we conducted whole‐genome resequencing on 206 individuals, each approximate 6‐fold depth coverage. And then employed three complementary approaches, including composite likelihood ratio, integrated haplotype homozygosity score detection runs homozygosity, detect signatures. In total, 10 genomic regions displaying were simultaneously identified by multiple methods, spanning 88.54 Mb. After annotating, these harbored collectively 92 unique genes. Interestingly, 32 reproduction distributed nine detected. Out them, two stood out as star candidates: BMPR1B GNRH2 , both which documented associations fertility, HOXA gene cluster ( HOXA1 ‐ 5 HOXA9 HOXA10 HOXA11 HOXA13 ) had also been linked fertility. Additionally, other that related hair follicle development LAMTOR3 EEF1A2 ), ear size KCNQ2 fat tail formation growth FAF1 CCNDBP1 GJB2 GJA3 deposition ACOXL JAZF1 HOXA3 HOXA4 HOXA5 EBF4 immune UBR1 FASTKD5 feed intake DAPP1 RNF17 NPBWR2 ). Our results offer novel insights genetic mechanisms underlying reference improvement programs.

Language: Английский

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Seminal plasma untargeted metabolomic and lipidomic profiling for the identification of a novel panel of biomarkers and therapeutic targets related to male infertility

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 27, 2023

Male infertility occurs approximately in about 50% of all cases and represents a serious concern worldwide. Traditional semen analysis alone is insufficient to diagnose male infertility. Over the past two decades, advances omics technologies have led widespread application metabolomics profiling as valuable diagnostic tool for various diseases disorders. Seminal plasma rich easily accessible source metabolites surrounding spermatozoa, milieu that provides several indispensable nutrients sustain sperm motility fertilization. Changes metabolic profiles seminal reflect reproductive tract Here, we performed lipidomics identify new pattern biomarkers samples from unfertile subjects ( n = 31) fertile controls 19) were analyzed using an untargeted metabolomics/lipidomics integrated approach, based on Ultra-High-Pressure Liquid Chromatography–tandem Mass Spectrometry. Partial Least Squares-Discriminant Analysis showed distinct separation between healthy men infertile subjects. Among 15 selected candidate Variable Importance Projection scores, phosphatidylethanolamine (PE) (18:1; 18:1) resulted with highest score. In total, 40 molecular species statistically significant variations men. Heat-map volcano plot indicated acylcarnitines, phosphatidylserine (PS) (40:2) lactate decreased, while PE 18:1), Phosphatidic acid (PA) (O-19:2; Lysophosphatidylethanolamine (LPE) (O-16:1) Phosphatidylcholine (PC) (O-16:2; 18:1)-CH3 increased group. The present study first one analyze dysregulation individuals regardless sub-infertility condition. Association metabolites/lipids reinforced data previous studies different approaches. particular, confirmed significantly decreased levels PS carnitines patients well positive correlation morphology. If validated larger prospective cohort, metabolite identified might inform novel strategies diagnosis interventions overcome

Language: Английский

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Estimates of genomic inbreeding and identification of candidate regions that differ between Chinese indigenous sheep breeds

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Journal Year: 2021, Volume and Issue: 12(1)

Published: Aug. 5, 2021

Abstract Background A run of homozygosity (ROH) is a consecutive tract homozygous genotypes in an individual that indicates it has inherited the same ancestral haplotype from both parents. Genomic inbreeding can be quantified based on ROH. regions enriched with ROH may indicative selection sweeps and are known as islands. We carried out analyses five Chinese indigenous sheep breeds; Altay ( n = 50 individuals), Large-tailed Han 50), Hulun Buir 150), Short-tailed grassland Tibetan using Ovine Infinium HD SNP BeadChip. Results total 18,288 were identified. The average number per across breeds ranged 39 (Hulun sheep) to 78 (Large-tailed length 0.929 Mb 2.544 sheep). effective population size Ne ) sheep, estimated 81, 78, 253, 238 70 generations ago. highest ROH-based estimate F was 0.0808 whereas lowest 0.0148 sheep. Furthermore, proportion long fragments (> 5 Mb) observed breed which indicated recent inbreeding. In total, 49 islands (the top 0.1% SNPs most commonly ROH) identified breeds. Three common all breeds, located OAR2: 12.2–12.3 Mb, OAR12: 78.4–79.1 OAR13: 53.0–53.6 Mb. breed-specific (OAR15: 3.4–3.8 Mb), (ORA17: 53.5–53.8 (ORA5:19.8–20.2 Mb). Collectively, harbored unique genes, including 19 genes have been documented having associations tail types, adaptation, growth, body size, reproduction or immune response. Conclusion Different patterns reflected their different histories. had genomic coefficients indicating Candidate could used illustrate genetic characteristics these Our findings contribute understanding diversity demography, help design implement breeding conservation strategies for

Language: Английский

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Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7492 - 7492

Published: July 8, 2024

Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile two subsets differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal by size-exclusion chromatography characterized an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for analysis. A total 157 lipid species 14 classes 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, sterols) were identified. Qualitative differences limited cholesteryl ester present only in S-sEVs. L-sEVs had higher levels all quantified due their larger membrane surface area. The distribution pattern different, especially sphingomyelins (more S-sEVs) ceramides L-sEVs). In conclusion, this reveals suggesting they differ biogenesis functionality.

Language: Английский

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Haplotypes affecting stillbirth and fertility in Icelandic Dairy Cattle

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

In the last decades, rate of stillbirths in Icelandic Dairy Cattle population has increased. Some these may be caused by recessive lethal mutations segregating population. These alleles can identified detecting homozygous haplotype deficiency (HHD) genotyped animals. The aim this study was to find genomic regions affecting stillbirth and fertility We analysed genotypes from 20,557 animals with 35,481 autosomal markers. HHD segments estimated their effects on insemination failure, measured as non-return rates. conducted genome-wide association studies (GWAS) for five traits: interval first inseminations, conception rate, number calving infertility. While no GWAS reached significance threshold, some top signals co-located haplotypes. A total 19 haplotypes significantly either decreased fertility, or increased incidence stillbirths, both. Two BTA13: 43,577,221 − 59,026,521 BTA8: 83,276,598 84,472,391 were associated both lower higher stillbirths. found evidence large structural variations around regions, suggesting that are likely due single loss-of-function mutation small variations. Further research should focus exploring using whole genome sequence data.

Language: Английский

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Role of Melatonin in Bovine Reproductive Biotechnology

Molecules, Journal Year: 2023, Volume and Issue: 28(13), P. 4940 - 4940

Published: June 23, 2023

Melatonin has profound antioxidant activity and numerous functions in humans as well livestock poultry. Additionally, melatonin plays an important role regulating the biological rhythms of animals. Combining with scientific breeding management considerable potential for optimizing animal physiological functions, but this idea still faces significant challenges. In review, we summarized beneficial effects supplementation on physiology reproductive processes cattle, including granulosa cells, oocytes, circadian rhythm, stress, inflammation, testicular function, spermatogenesis, semen cryopreservation. There is much emerging evidence that can profoundly affect cattle. future, hope not only be applied to also used safely effectively improve efficiency husbandry.

Language: Английский

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Genomic insights into key genes and QTLs involved in cattle reproduction

Gene, Journal Year: 2024, Volume and Issue: 917, P. 148465 - 148465

Published: April 14, 2024

Language: Английский

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The pair ceramide 1-phosphate/ceramide kinase regulates intracellular calcium and progesterone-induced human sperm acrosomal exocytosis

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: March 31, 2023

Before fertilization, spermatozoa must undergo calcium-regulated acrosome exocytosis in response to physiological stimuli such as progesterone and zona pellucida. Our laboratory has elucidated the signaling cascades accomplished by different sphingolipids during human sperm acrosomal exocytosis. Recently, we established that ceramide increases intracellular calcium activating various channels stimulating reaction. However, whether induces on its own, activation of kinase/ceramide 1-phosphate (CERK/C1P) pathway or both is still an unsolved issue. Here, demonstrate C1P addition intact, capacitated sperm. Real-time imaging single-cell measurements population showed needs extracellular induce [Ca2+]i increase. The sphingolipid triggered cation influx through voltage-operated (VOC) store-operated (SOC) channels. it requires efflux from internal stores inositol 3-phosphate receptors (IP3R) ryanodine (RyR) achieve rise We report presence CERK spermatozoa, enzyme catalyzes synthesis. Furthermore, exhibited calcium-stimulated enzymatic activity Exocytosis assays using a inhibitor demonstrated exocytosis, mainly due Strikingly, required increase This first report, implicating bioactive leading

Language: Английский

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