Sperm Selection for ICSI: Do We Have a Winner?

Cells, Journal Year: 2021, Volume and Issue: 10(12), P. 3566 - 3566

Published: Dec. 17, 2021

In assisted reproductive technology (ART), the aim of sperm cells’ preparation is to select competent spermatozoa with highest fertilization potential and in this context, intracytoplasmic injection (ICSI) represents most applied technique for fertilization. This makes process identifying perfect extremely important. A number methods have now been developed mimic some natural selection processes that exist female tract. Although many studies conducted identify election technique, doubts disagreements still remain. review, we will discuss all cell techniques currently available ICSI, starting from basic methodologies continuing those suitable cells reduced motility. Furthermore, different exploit membrane characteristics advanced strategy based on microfluidics, be examined. Finally, a new method micro swim-up directly ICSI dish analyzed. Eventually, advantages disadvantages each debated, trying draw reasonable conclusions their efficacy order establish gold standard method.

Language: Английский

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Metabolic adaptation mechanisms of glycogen reduction and lipid accumulation in testicular protection in Daurian ground squirrels during hibernation

Biochimie, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: March 30, 2023

Whether basal metabolic activity in sperm has any influence on their fertilising capacity not been explored. Using the pig as a model, present study investigated relationship of energetic metabolism with quality and function (assessed through computer-assisted analysis flow cytometry), fertility (in vitro fertilisation (IVF) outcomes). In semen samples from 16 boars, levels metabolites related to glycolysis, ketogenesis Krebs cycle were determined targeted metabolomics approach using liquid chromatography-tandem mass spectrometry. High-quality are associated greater glycolysis-derived metabolites, oocyte embryo development conditioned by status. Interestingly, glycolysis appears be preferred catabolic pathway giving rise percentages embryos at day 6. conclusion, this shows that influences function, even beyond fertilisation.

Language: Английский

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ATP10A deficiency results in male-specific infertility in mice

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Feb. 13, 2024

Over 8% of couples worldwide are affected by infertility and nearly half these cases due to male-specific issues where the underlying cause is often unknown. Therefore, discovery new genetic factors contributing in model organisms can enhance our understanding etiology this disorder. Here we show that murine ATP10A, a phospholipid flippase, highly expressed male reproductive organs, specifically testes vas deferens. tested influence ATP10A on reproduction examining fertility Atp10A knockout mice. Our findings reveal deficiency leads infertility, but does not perturb females. The deficient mice exhibit smaller testes, reduced sperm count (oligozoospermia) lower motility (asthenozoospermia). Additionally, display deferens histopathological abnormalities, as well altered total relative amounts hormones associated with hypothalamic-pituitary-gonadal axis. Surprisingly, circulating testosterone elevated 2-fold while luteinizing hormone, follicle stimulating inhibin B levels were significantly different from WT littermates. also gonadotropin receptors alterations ERK, p38 MAPK, Akt, cPLA 2 -dependent signaling testes. was knocked out C57BL/6J background, which carries an inactivating nonsense mutation closely related lipid Atp10D. We have corrected Atp10D using CRISPR/Cas9 determined loss alone sufficient Collectively, highlight critical role provide valuable insights into molecular mechanisms.

Language: Английский

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From oxidative imbalance to compromised standard sperm parameters: Toxicological aspect of phthalate esters on spermatozoa

Environmental Toxicology and Pharmacology, Journal Year: 2023, Volume and Issue: 98, P. 104085 - 104085

Published: Feb. 23, 2023

Language: Английский

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Seminal plasma untargeted metabolomic and lipidomic profiling for the identification of a novel panel of biomarkers and therapeutic targets related to male infertility

Frontiers in Pharmacology, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 27, 2023

Male infertility occurs approximately in about 50% of all cases and represents a serious concern worldwide. Traditional semen analysis alone is insufficient to diagnose male infertility. Over the past two decades, advances omics technologies have led widespread application metabolomics profiling as valuable diagnostic tool for various diseases disorders. Seminal plasma rich easily accessible source metabolites surrounding spermatozoa, milieu that provides several indispensable nutrients sustain sperm motility fertilization. Changes metabolic profiles seminal reflect reproductive tract Here, we performed lipidomics identify new pattern biomarkers samples from unfertile subjects ( n = 31) fertile controls 19) were analyzed using an untargeted metabolomics/lipidomics integrated approach, based on Ultra-High-Pressure Liquid Chromatography–tandem Mass Spectrometry. Partial Least Squares-Discriminant Analysis showed distinct separation between healthy men infertile subjects. Among 15 selected candidate Variable Importance Projection scores, phosphatidylethanolamine (PE) (18:1; 18:1) resulted with highest score. In total, 40 molecular species statistically significant variations men. Heat-map volcano plot indicated acylcarnitines, phosphatidylserine (PS) (40:2) lactate decreased, while PE 18:1), Phosphatidic acid (PA) (O-19:2; Lysophosphatidylethanolamine (LPE) (O-16:1) Phosphatidylcholine (PC) (O-16:2; 18:1)-CH3 increased group. The present study first one analyze dysregulation individuals regardless sub-infertility condition. Association metabolites/lipids reinforced data previous studies different approaches. particular, confirmed significantly decreased levels PS carnitines patients well positive correlation morphology. If validated larger prospective cohort, metabolite identified might inform novel strategies diagnosis interventions overcome

Language: Английский

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Identification of protein candidates in spermatozoa of water buffalo (Bubalus bubalis) bulls helps in predicting their fertility status

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 20, 2023

The water buffalo (Bubalus bubalis) is an indispensable part of the Indian dairy sector and in several instances, farmers incur economic losses due to failed pregnancy after artificial insemination (AI). One key factors for failure conception use semen from bulls low fertilizing potential hence, it becomes important predict fertility status before performing AI. In this study, global proteomic profile high fertile (HF) (LF) bull spermatozoa was established using a high-throughput LC-MS/MS technique. A total 1,385 proteins (≥1 high-quality PSM/s, ≥1 unique peptides, p < 0.05, FDR 0.01) were identified out which, 1,002 common between both HF LF groups while 288 95 respectively. We observed 211 342 significantly (log Fc ≥ 2) abundant ≤ 0.5) (p 0.05). Gene ontology analysis revealed that associated involved spermatogenesis, sperm motility, acrosome integrity, zona pellucida binding other functions. Besides this, glycolysis, fatty acid degradation inflammation. Furthermore, related differentially (DAPs) on viz., AKAP3, Sp17, DLD validated through Western blotting immunocytochemistry which coherence with data. DAPs study may be used as protein candidates predicting buffaloes. Our findings provide opportunity mitigating male infertility.

Language: Английский

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New horizons in human sperm selection for assisted reproduction

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 22, 2023

Male infertility is a commonly encountered pathology that estimated to be contributory factor in approximately 50% of couples seeking recourse assisted reproductive technologies. Upon clinical presentation, such males are subjected conventional diagnostic andrological practices rely on descriptive criteria define their fertility based the number morphologically normal, motile spermatozoa within ejaculate. Despite virtual ubiquitous adoption practices, they not without limitations and accordingly, there now increasing awareness importance assessing sperm quality order more accurately predict male's status. This realization raises important question which characteristics signify high-quality, fertilization competent cell. In this review, we reflect recent advances our mechanistic understanding biology function, contributing growing armory innovative approaches diagnose treat male infertility. particular review progress toward implementation precision medicine; robust setting fertility, currently lags well behind other fields medicine. this, research shows application advanced technology platforms as whole exome sequencing proteomic analyses hold considerable promise optimizing outcomes for management by uncovering expanding inventory candidate biomarkers, those associated with recurrent pregnancy loss. Similarly, development imaging technologies tandem machine learning artificial intelligence poised disrupt care paradigm advancing molecular biological causes provide novel avenues future diagnostics treatments.

Language: Английский

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Effects of Trehalose Supplementation on Lipid Composition of Rooster Spermatozoa Membranes in a Freeze/Thaw Protocol

Animals, Journal Year: 2023, Volume and Issue: 13(6), P. 1023 - 1023

Published: March 10, 2023

The plasma membrane of spermatozoa plays an important role in the formation and maintenance many functions spermatozoa, including during cryopreservation. As a result chromatographic analysis, content lipids fatty acids membranes roosters two breeds was determined under influence cryoprotective media containing trehalose LCM-control (0 mM), Treh20 (9.5 Treh30 (13.4 mM). use diluent made it possible to maintain dynamic balance between synthesis degradation phospholipids sterols frozen/thawed close that native spermatozoa. This contributed increase preservation from 48.3% 52.2% egg breed 30.0% 35.1% meat- and-egg breed. It also noted their kinetic apparatus (mobility indicators) remained at level 45.6% (egg breed) 52.4% (meat-and-egg breed). An concentration 13.4 mM for rooster sperm resulted decrease morphofunctional parameters

Language: Английский

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Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7492 - 7492

Published: July 8, 2024

Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile two subsets differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal by size-exclusion chromatography characterized an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for analysis. A total 157 lipid species 14 classes 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, sterols) were identified. Qualitative differences limited cholesteryl ester present only in S-sEVs. L-sEVs had higher levels all quantified due their larger membrane surface area. The distribution pattern different, especially sphingomyelins (more S-sEVs) ceramides L-sEVs). In conclusion, this reveals suggesting they differ biogenesis functionality.

Language: Английский

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