LncRNA MALAT1-deficiency restrains lipopolysaccharide (LPS)-induced pyroptotic cell death and inflammation in HK-2 cells by releasing microRNA-135b-5p DOI Creative Commons
Jie Huang, Xu Chen

Renal Failure, Journal Year: 2021, Volume and Issue: 43(1), P. 1288 - 1297

Published: Jan. 1, 2021

Long non-coding RNAs (LncRNAs) participate in the regulation of chronic kidney disease (CKD), and acute injury (AKI) is identified as an important risk factor for CKD. This study investigated involvement a novel LncRNA MALAT1 regulating lipopolysaccharide (LPS)-induced cell pyroptosis inflammation human renal tubular epithelial HK-2 cells. Here, cells were subjected to LPS (2 μg/mL) treatment establish cellular AKI models vitro, we validated that triggered NLRP3-mediated pyroptotic death, promoted apoptosis inflammation-associated cytokines secretion induce injury. Then, MALAT1/miRNA (miRNA)-135b-5p axis was verified rescue viability treated by targeting NLRP3. Mechanistically, miRNA-135b-5p bound MALAT1, positively regulated NLRP3 through acting RNA sponger miRNA-135b-5p. Further gain- loss-of-function experiments evidenced both ablation overexpression reversed LPS-induced pyroptosis, apoptosis, cells, protective effects knock-down on LPS-treated abrogated silencing In general, our firstly role MALAT1/ miRNA-135b-5p/NLRP3 signaling cascade inflammatory death

Language: Английский

Abnormal metabolism in hepatic stellate cells: Pandora's box of MAFLD related hepatocellular carcinoma DOI

Yuan-dong Sun,

Hao Zhang, Yuanmin Li

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2024, Volume and Issue: 1879(2), P. 189086 - 189086

Published: Feb. 9, 2024

Language: Английский

Citations

7
Assessment of liver fibrosis progression and regression by a serological collagen turnover profile DOI Open Access
M.A. Karsdal,

Sara Toftegaard Hjuler,

Yi Luo

et al.

AJP Gastrointestinal and Liver Physiology, Journal Year: 2018, Volume and Issue: 316(1), P. G25 - G31

Published: Aug. 30, 2018

There is a need for noninvasive biomarkers that can identify patients with progressive liver fibrosis and monitor response to antifibrotic therapy. An equally important identification of spontaneous regression, since they may not treatment nor be included in clinical studies as end point. Circulating biomarkers, originating from defined fragments the scar tissue itself, serve valuable tools this aspect precision medicine. We investigated panel serological collagen formation degradation markers likely regress or progress absence therapeutic intervention. Plasma samples moderate-stage hepatitis C receiving placebo phase II trial peroxisome proliferator-activated receptor agonist farglitazar were included. The had matched biopsies at baseline 52 wk follow-up. Serological (PRO-C3, PRO-C4, PRO-C5) (C3M, C4M, C6M) analyzed. Logistic regression analysis including PRO-C3 C6M identified subjects an AUROC 0.91 ( P < 0.0001) positive negative predictive values (PPV/NPV) 75.0%/88.6%. Low levels PRO-C5 predicted phenotype, odds ratio 33.8 times higher compared high 0.0025) 0.78 PPV/NPV 60.0%/95.7%. Two (PRO-C3 progressors, one fragment (PRO-C5) regressors. These improve patient stratification efficacy NEW & NOTEWORTHY In study we report two are able progressors while biomarker particular, present three used fibrosis, therapy, also phenotype.

Language: Английский

Citations

49
Nonalcoholic Fatty Liver Disease (NAFLD): Pathogenesis and Noninvasive Diagnosis DOI Creative Commons

Vicneswarry Dorairaj,

Siti Aishah Sulaiman,

Nadiah Abu

et al.

Biomedicines, Journal Year: 2021, Volume and Issue: 10(1), P. 15 - 15

Published: Dec. 22, 2021

The global prevalence of nonalcoholic fatty liver disease (NAFLD) or metabolic associated (MAFLD), as it is now known, has gradually increased. NAFLD a with spectrum stages ranging from simple (steatosis) to severe form steatosis, steatohepatitis (NASH), which could progress irreversible injury (fibrosis) and organ failure, in some cases hepatocellular carcinoma (HCC). Although biopsy remains the gold standard for accurate detection this condition, unsuitable clinical screening due higher risk death. There thus an increased need find alternative techniques tools diagnosis. Early NASH matters patients because marker progression. This review summarizes current noninvasive diagnosis their performance. We also discussed potential newer diagnosing NAFLD.

Language: Английский

Citations

40
TUG1 protects against ferroptosis of hepatic stellate cells by upregulating PDK4-mediated glycolysis DOI

Xiangting Zhang,

Luying Zhao,

Kanglei Ying

et al.

Chemico-Biological Interactions, Journal Year: 2023, Volume and Issue: 383, P. 110673 - 110673

Published: Aug. 13, 2023

Language: Английский

Citations

15
Screening and identification of potential biomarkers for pancreatic cancer: An integrated bioinformatics analysis DOI

Somayeh Jafari,

Milad Ravan, Iman Karimi-Sani

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 249, P. 154726 - 154726

Published: Aug. 2, 2023

Language: Английский

Citations

14
Non‑coding RNA: A promising diagnostic biomarker and therapeutic target for esophageal squamous cell carcinoma (Review) DOI Open Access
Longze Zhang, Yanyan Wang, Jianmei Gao

et al.

Oncology Letters, Journal Year: 2024, Volume and Issue: 27(6)

Published: April 9, 2024

Esophageal cancer (EC) is a common form of malignant tumor in the digestive system that classified into two types: squamous cell carcinomas (ESCC) and esophageal adenocarcinoma. ESCC known for its early onset symptoms, which can be difficult to identify, as well rapid progression tendency develop drug resistance chemotherapy radiotherapy. These factors contribute high incidence disease low cure rate. Therefore, diagnostic biomarker therapeutic target need identified ESCC. Non‑coding RNAs (ncRNAs) are class molecules transcribed from DNA but do not encode proteins. Initially, ncRNAs were considered non‑functional segments generated during transcription. However, with advancements high‑throughput sequencing technologies recent years, have been associated poor prognosis, The present study provides comprehensive overview biogenesis, characteristics functions ncRNAs, particularly focusing on microRNA, long circular RNAs. Furthermore, could potentially used biomarkers targets summarized highlight their application value prospects

Language: Английский

Citations

5
Expression of the Long Noncoding RNA GAS5 Correlates with Liver Fibrosis in Patients with Nonalcoholic Fatty Liver Disease DOI Open Access
Man‐Hoon Han, Jee Hyun Lee, Gyeonghwa Kim

et al.

Genes, Journal Year: 2020, Volume and Issue: 11(5), P. 545 - 545

Published: May 13, 2020

Background: Advanced liver fibrosis is the most important prognostic factor in nonalcoholic fatty disease (NAFLD). The long noncoding RNA (lncRNA), growth arrest-specific transcript 5 (GAS5), associated with inhibition of fibrogenesis, and its levels are decreased cirrhotic liver. Methods: We analyzed 51 patients NAFLD, diagnosis which was confirmed by biopsy. Expression GAS5 both plasma using a quantitative real-time polymerase chain reaction according to stage. Results: Plasma expression significantly higher advanced than those without. As progressed, increased, exception that livers. were lower cirrhosis fibrosis. Conclusion: Elevated circulating lncRNA progression prior development cirrhosis.

Language: Английский

Citations

37
Bone marrow mesenchymal stem cells inhibit hepatic fibrosis via the AABR07028795.2/rno-miR-667-5p axis DOI Creative Commons
Yuan Feng, Yanjie Li,

Mingxing Xu

et al.

Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 13(1)

Published: July 28, 2022

Abstract Background The mechanism of bone marrow mesenchymal stem cells (BMSCs) in treating hepatic fibrosis remains unclear. Methods TGF-β1-induced stellate cell (HSC)-T6 and CCl4-induced rats were treated with BMSCs. HSC-T6 activity was determined using the counting kit-8 assay, histology change evaluated hematoxylin eosin Masson staining. expression markers real-time quantitative PCR, Western blotting, immunocytochemistry. RNA sequencing (RNA-seq) used to screen lncRNAs involved effect BMSCs fibrosis, function fibrosis-associated lncRNA marker investigated. potential miRNA target predicted R software. interaction between verified luciferase report system immunoprecipitation (RIP) 293T cells. Results BMSC attenuated activation suppressed gene (MMP2, Collagen I, αSMA) at transcription translation levels. treatment also improves by decreasing genes suppressing collagen deposition liver. RNA-seq revealed that AABR07028795.2 (lnc-BIHAA1) downregulated after as compared those HSC-T6, subsequently, functional analysis showed lnc-BIHAA1 plays a beneficial role fibrosis. Luciferase assay RIP interacted miRNA, rno-miR-667-5p, functioning phenotype suppressor HSC-T6. Moreover, overexpression rno-miR-667-5p significantly reverses on Conclusions suppresses downregulating lnc-BIHAA1/rno-miR-667-5p signaling pathway HSCs. Our results provide scientific basis for establishing biological method liver

Language: Английский

Citations

19
Salvianolic acid B suppresses hepatic stellate cell activation and liver fibrosis by inhibiting the NF-κB signaling pathway via miR-6499-3p/LncRNA-ROR DOI
Rong Wang, Shengnan Li, Panpan Chen

et al.

Phytomedicine, Journal Year: 2022, Volume and Issue: 107, P. 154435 - 154435

Published: Sept. 6, 2022

Language: Английский

Citations

18
Dysregulation of murine long noncoding single-cell transcriptome in nonalcoholic steatohepatitis and liver fibrosis DOI

Kritika Karri,

David J. Waxman

RNA, Journal Year: 2023, Volume and Issue: 29(7), P. 977 - 1006

Published: April 4, 2023

LncRNAs comprise a heterogeneous class of RNA-encoding genes typified by low expression, nuclear enrichment, high tissue-specificity, and functional diversity, but the vast majority remain uncharacterized. Here, we assembled mouse liver noncoding transcriptome from >2000 bulk RNA-seq samples discovered 48,261 liver-expressed lncRNAs, novel. Using these lncRNAs as single-cell transcriptomic reference set, elucidated lncRNA dysregulation in models fat diet-induced nonalcoholic steatohepatitis carbon tetrachloride-induced fibrosis. Trajectory inference analysis revealed zonation patterns across lobule each major cell population. Perturbations expression were common several disease-associated types, including steatohepatitis-associated macrophages, hallmark fatty disease progression, collagen-producing myofibroblasts, central feature Single-cell-based gene regulatory network using bigSCale2 linked individual to specific biological pathways, network-essential with functions identified their centrality metrics. For subset promoter sequences network-defined target significantly enriched for triplex formation, providing independent mechanistic support lncRNA–target linkages predicted networks. These findings elucidate cell-type specificities, spatial patterns, associated networks, temporal during hepatic progression. A have human orthologs are promising candidates biomarkers therapeutic targets.

Language: Английский

Citations

11