Spectacular role of epididymis and bio-active cargo of nano-scale exosome in sperm maturation: A review

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 164, P. 114889 - 114889

Published: May 18, 2023

The epididymis is responsible for post-testicular sperm maturation as it provides a favorable environment spermatozoa to gain the ability movement and fertilization. recent evidence has shown that, are vulnerable dynamic variations driven by various cellular exposure mechanisms mediated epididymosomes. Exosomes provide new insight into mechanism of intercellular communication because they direct transfer several important bio-active cargo elements (proteins, lipid, DNA, mRNA, microRNA, circular RNA, long noncoding RNA) between spermatozoa. In broad sense, proteomic analysis exosomes from indicates number proteins that involved in motility, acrosomal reaction, prevent pre-mature capacitation male infertility. Pinpointing, how reproductive disorders associated with nano-scale exosome tract. Therefore, current review presents regarding distinctive characteristics functions tract both pathological physiological developments, argue these vesicles serve an regulator reproduction, fertility, disease susceptibility.

Language: Английский

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Zinc and Its Impact on the Function of the Testicle and Epididymis

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 8991 - 8991

Published: Aug. 19, 2024

Zinc (Zn) is an essential trace element; it exhibits a plethora of physiological properties and biochemical functions. It plays pivotal role in regulating the cell cycle, apoptosis, DNA organization, as well protein, lipid, carbohydrate metabolism. Among other important processes, Zn reproductive health. The ZIP ZnT proteins are responsible for mobilization within cell. inert antioxidant through its interaction with variety enzymes to regulate redox system, including metallothioneins (MTs), metalloenzymes, gene regulatory proteins. system great importance; such spermatogenesis sperm maturation that occur testicle epididymis, respectively, depend on this element their development function. modulates synthesis androgens, testosterone, these so deficiency related alterations parameters lead male infertility.

Language: Английский

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Contemporary Use of ICSI and Epigenetic Risks to Future Generations

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(8), P. 2135 - 2135

Published: April 11, 2022

Since the birth of Louise Brown in 1978 via IVF, reproductive specialists have acquired enormous knowledge and refined several procedures, which are nowadays applied assisted technology (ART). One most critical steps this practice is fertilization process. In early days a remarkable concern was unpleasant outcomes failed fertilization, overtaken by introducing intracytoplasmic sperm injection (ICSI), delineating real breakthrough modern ART. ICSI became standard soon used as common method to fertilize oocytes. It has been for severe male factor infertility non-male factors, such unexplained or advanced maternal age, without robust scientific evidence. However, applying blindly not free potential detrimental consequences since novel studies report possible health offspring. DNA methylation epigenetic alterations cells infertile men might help explain some adverse effects reported on future generations. Collected data concerning children over past thirty years seems support notion that there be an increased risk disorders, congenital malformations, chromosomal alterations, subfertility babies born following compared naturally conceived children. it still elucidated what level these associated with cause technique. This review provides overview mechanisms imprinting use ART, particular ICSI. also highlights contribution embryo regulation risks vitro culture conditions dysregulation. Lastly, summarizes literature disorders after

Language: Английский

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Arboviruses and symbiotic viruses cooperatively hijack insect sperm-specific proteins for paternal transmission

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 9, 2023

Abstract Arboviruses and symbiotic viruses can be paternally transmitted by male insects to their offspring for long-term viral persistence in nature, but the mechanism remains largely unknown. Here, we identify sperm-specific serpin protein HongrES1 of leafhopper Recilia dorsalis as a mediator paternal transmission reovirus Rice gall dwarf virus (RGDV) previously undescribed Virgaviridae family, filamentous (RdFV). We show that mediates direct binding virions sperm surfaces subsequent via interaction with both capsid proteins. Direct proteins simultaneously invasion two into reproductive organs. Moreover, arbovirus activates expression suppress conversion prophenoloxidase active phenoloxidase, potentially producing mild antiviral melanization defense. Paternal scarcely affects fitness. These findings provide insights how different cooperatively hijack insect without disturbing functions.

Language: Английский

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Melatonin alleviated fluoride-induced impairment of spermatogenesis and sperm maturation process via Interleukin-17A

Food and Chemical Toxicology, Journal Year: 2023, Volume and Issue: 178, P. 113867 - 113867

Published: June 1, 2023

Language: Английский

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Sperm Metabolism

Molecular Reproduction and Development, Journal Year: 2024, Volume and Issue: 91(10)

Published: Oct. 1, 2024

ABSTRACT Bioenergetics plays a crucial role in sperm functions, including motility, capacitation‐related protein modifications, oocyte recognition and interaction, all of which are essential for fertilization. Sperm metabolism is recognized as flexible, responding to environmental cues energetic demands during ejaculation, the journey along female tract, until Recent studies suggest that metabolic functions relevant beyond fertilization may influence zygote embryo development, impacting paternal‐derived effects on offspring development health. In recent years, homeostasis have gained increasing interest male reproduction research. Given implications fertility‐related processes, this field not only human fertility but also livestock research, semen conservation, assisted reproductive techniques. Newly developed assessment tools allowing better understanding under different conditions identifying species‐specific peculiarities. This review aims discuss current knowledge mammalian metabolism, focusing features, changes journey, potential contributions translational research biotechnologies. Furthermore, we propose future perspectives bioenergetics

Language: Английский

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Association of seminal plasma zinc levels with human semen quality and its toxic effects on sperm motility

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 284, P. 116889 - 116889

Published: Aug. 25, 2024

Infertility has become one of the most common chronic diseases among reproductive- aged individuals, and male factors account for about 50 %. Zinc is a trace element that essential sperm quality reproductive system. Although several current studies with small sample sizes have investigated this relationship, conclusions been still controversial. Here, using large population involved 25,915 participants from Changzhou Maternity Child Health Care Hospital, we revealed an inverted "U"-shaped trend between seminal plasma zinc concentrations motility PR/PR + NP (PR, progressive motility; NP, non-progressive motility). The results showed highest values observed in group intermediate (Group 2, 0.25-2.11 mmol/L). mean were 43.17 ± 19.03 % 56.64 20.28 %, respectively. And lowest came out levels 4, > 3.04 In vitro cell experiments also caused dose-dependent cytotoxicity GC-2 cells at threshold value. Furthermore, RNA-seq analysis demonstrated high exerted toxic effects on through immune injury. Taken together, our findings suggested moderate amounts are crucial human reproduction excessive may adverse fertility.

Language: Английский

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Subchronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(47)

Published: Nov. 11, 2024

Forecasted increases in the prevalence and severity of extreme weather events accompanying changes climatic behavior pose potential risk to reproductive capacity humans animals ecological agricultural significance. While several studies have revealed that heat stress induced by challenges such as testicular insulation can elicit a marked negative effect on male system, particularly production spermatozoa, less is known about immediate impact function following subchronic whole-body exposure elevated ambient temperature. To address this knowledge gap, we exposed unrestrained mice conditions emulate wave (daily cycle 8 h at 35 °C followed 16 25 °C) for period 7 d. Neither testes or epididymides heat-exposed exhibited evidence gross histological change, similarly, spermatozoa males retained their functionality ability support embryonic development. However, embryos generated from experienced pronounced gene expression linked acceleration early embryo development, aberrant blastocyst hatching, increased fetal:placental weight ratio. Such were causally associated with an altered sperm small noncoding RNA (sncRNA) profile, these developmental phenotypes recapitulated microinjection wild-type sired control RNAs extracted spermatozoa. data highlight even relatively modest excursions temperature affect identify sncRNA profile particular point vulnerability imposed environmental stress.

Language: Английский

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Sperm Ion Transporters and Channels in Human Asthenozoospermia: Genetic Etiology, Lessons from Animal Models, and Clinical Perspectives

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(7), P. 3926 - 3926

Published: April 1, 2022

In mammals, sperm fertilization potential relies on efficient progression within the female genital tract to reach and fertilize oocyte. This fundamental property is supported by flagellum, an evolutionarily conserved organelle that provides mechanical force for propulsion motility. Importantly several functional maturation events occur during journey of cells through tracts are necessary activation flagellar beating acquisition potential. Ion transporters channels located at surface have been demonstrated be involved in these processes, particular, downstream signaling pathways promotion novel biochemical electrophysiological properties cells. We performed a systematic literature review describe currently known genetic alterations humans affect ion result asthenozoospermia, pathophysiological condition defined reduced or absent motility observed nearly 80% infertile men. also present physiological relevance mechanisms additional identified mouse. Finally, considering state-of-the art, we discuss future perspectives terms therapeutics asthenozoospermia male contraception.

Language: Английский

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Male Sex Hormones, Metabolic Syndrome, and Aquaporins: A Triad of Players in Male (in)Fertility

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 1960 - 1960

Published: Jan. 19, 2023

Infertility is becoming a chronic and emerging problem in the world. There resistant stigma that this health condition mostly due to female, although literature supports responsibility for onset of infertility equally shared between both sexes more or less equal proportions. Nevertheless, male sex hormones, particularly testosterone (T), are key players male-related infertility. Indeed, hypogonadism, which also characterized by changes T levels, one most common causes its incidence has been interconnected increased prevalence metabolic diseases. Recent data highlight role aquaporin (AQP)-mediated water solute diffusion homeostasis testicular cells suggesting strong correlation AQPs function, metabolism cells, recent studies showed sexual hormone concentrations can change expression pattern function AQPs. Herein, we review up-to-date information on involvement AQP-mediated permeability men with syndrome deficit, highlighting putative mechanisms show an interaction AQPs, may contribute

Language: Английский

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