Lab on a Chip,
Journal Year:
2023,
Volume and Issue:
23(19), P. 4265 - 4275
Published: Jan. 1, 2023
The
CRISPR
Cas
system,
as
a
novel
nucleic
acid
detection
tool,
is
often
hindered
by
cumbersome
experimental
procedures,
complicated
reagent
transfer
processes,
and
associated
aerosol
pollution
risks.
In
this
study,
an
integrated
platform
named
"up
down
chip"
was
developed,
which
combined
RT-RAA
technology
for
amplification,
DNase-dead
Cas12a-modified
magnetic
beads
specific
recognition
of
target
acid,
HRP-TMB
chromogenic
reaction
signal
output
in
different
chambers
single
microfluidic
chip.
were
migrated
up-and-down
manner
between
through
driving,
achieving
"sample-in,
result-out"
mode.
By
introducing
homemade
heating
box
temperature
control
during
the
using
naked
eye
or
smartphone
APP
color-based
reading,
no
professional
precise
instruments
required
platform.
Using
platform,
highly
sensitive
HIV-1
genome
low
250
copies
(CPs)
per
mL
achieved
within
100
min
while
maintaining
good
performance
against
common
variants
well
excellent
specificity
anti-interference
ability.
addition,
compared
with
qRT-PCR,
it
also
exhibited
accuracy
56
spiked
plasma
samples,
indicating
its
promising
potential
clinical
application.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2022,
Volume and Issue:
12
Published: Feb. 17, 2022
Hepatitis
C
virus
(HCV)
infection
is
a
global
public
health
threat.
Reaching
the
World
Health
Organization’s
objective
for
eliminating
viral
hepatitis
by
2030
will
require
precise
disease
diagnosis.
While
immunoassays
and
qPCR
play
significant
role
in
detecting
HCV,
rapid
accurate
point-of-care
testing
important
pathogen
identification.
This
study
establishes
reverse
transcription
recombinase-aided
amplification–lateral
flow
dipstick
(RT-RAA-LFD)
assay
to
detect
HCV.
The
intact
workflow
was
completed
within
30
min,
detection
limit
synthesized
C/E1
plasmid
gene-containing
10
copies/μl.
In
addition,
test
showed
good
specificity,
with
no
cross-reactivity
observed
A
virus,
B
HIV,
syphilis,
human
papillomavirus
virus.
Using
extracted
RNAs
from
46
anti-HCV
antibody-positive
samples,
RT-RAA-LFD
100%
positive
negative
concordance
rates
qPCR.
summary,
established
this
suitable
clinical
of
HCV
at
community
level
remote
areas.
Biosensors,
Journal Year:
2024,
Volume and Issue:
14(6), P. 265 - 265
Published: May 22, 2024
Traditional
single
nucleic
acid
assays
can
only
detect
one
target
while
multiple
targets
simultaneously,
providing
comprehensive
and
accurate
information.
Fluorescent
microspheres
in
multiplexed
detection
offer
high
sensitivity,
specificity,
multiplexing,
flexibility,
scalability
advantages,
enabling
precise,
real-time
results
supporting
clinical
diagnosis
research.
However,
face
challenges
like
complexity,
costs,
sample
handling
issues.
The
review
explores
the
recent
advancements
applications
of
fluorescent
detection.
It
discusses
versatility
various
fields,
such
as
disease
diagnosis,
drug
screening,
personalized
medicine.
highlights
possibility
adjusting
performance
by
modifying
concentrations
carrier
forms,
allowing
for
tailored
applications.
emphasizes
potential
microsphere
technology
revolutionizing
advancing
health,
treatment,
medical
Applied Physics Reviews,
Journal Year:
2023,
Volume and Issue:
10(3)
Published: Aug. 7, 2023
Mpox
is
an
infectious
viral
disease,
and,
as
of
January
12,
2023,
about
84
560
distinct
cases
have
been
detected,
involving
110
countries
since
May
2022.
Most
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)-based
detection
methods
require
long
assay
time
because
the
pre-amplification
to
target
nucleic
acids.
Herein,
we
designed
a
new
prototypal
mpox
biosensor
that
allows
amplification-free
acid
by
combining
CRISPR/Cas12b
system
and
ultrasensitive
graphene
field-effect
transistor
(gFET).
The
CRISPR/Cas12b–gFET
enabled
virus
(MPXV)
DNA
targets
at
sensitivity
∼1
aM
within
20
min.
We
several
sgRNAs
recognize
genes
MPXV
then
Cas12b
protein
cleaved
form
double-strand
break.
specificity
was
verified
among
other
homologous
Orthopoxviruses,
sgRNA
could
distinguish
between
recent
2022
West
African
with
mutation
site,
showing
its
potential
application
in
detection.
Thus,
demonstrated
CRISPR–gFET
technology
serve
sensitive
rapid
diagnostic
tool
for
viruses
future.
