Comprehensive transcriptomic analysis reveals turnip mosaic virus infection and its aphid vector Myzus persicae cause large changes in gene regulatory networks and co-transcription of alternative spliced mRNAs in Arabidopsis thaliana

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 30, 2025

Abstract Background Virus infection and herbivory can alter the expression of stress-responsive genes in plants. This study employed high-throughput transcriptomic alternative splicing analysis to understand separate combined impacts on host gene Arabidopsis thaliana by Myzus persicae (green peach aphid), turnip mosaic virus (TuMV). Results By investigating changes transcript abundance, data shows that aphids feeding infected plants intensify number differentially expressed stress responsive compared challenge individual stressors. presents evidence combination virus-vector-host interactions induces significant hormone secondary metabolite biosynthesis, as well downstream factors involved feedback loops within signaling pathways. also expressions are regulated through pre-mRNA use transcription start termination sites. Conclusions These suggest complex genetic occur adapt challenges posed viruses they vector. provides more advanced analyses could be used future dissect mechanisms mediating tripartite inform breeding programs.

Language: Английский

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ABA as a downstream signal actively participates in phthalanilic acid mediated cold tolerance of common beans (Phaseolus vulgaris)

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 220, P. 109594 - 109594

Published: Jan. 31, 2025

Language: Английский

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Non-coding RNAs fine-tune the balance between plant growth and abiotic stress tolerance

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 12, 2022

To survive in adverse environmental conditions, plants have evolved sophisticated genetic and epigenetic regulatory mechanisms to balance their growth abiotic stress tolerance. An increasing number of non-coding RNAs (ncRNAs), including small (sRNAs) long (lncRNAs) been identified as essential regulators which enable coordinate multiple aspects responses stresses through modulating the expression target genes at both transcriptional posttranscriptional levels. In this review, we summarize recent advances understanding ncRNAs-mediated prioritization towards plant or tolerance stresses, especially cold, heat, drought salt stresses. We highlight diverse roles evolutionally conserved microRNAs (miRNAs) interfering (siRNAs), underlying phytohormone-based signaling crosstalk regulating between also review current discoveries regarding potential ncRNAs memory plants, offer descendants for better fitness. Future ncRNAs-based breeding strategies are proposed optimize maximize crop yield under changing climate.

Language: Английский

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The autophagy receptor NBR1 directs the clearance of photodamaged chloroplasts

eLife, Journal Year: 2023, Volume and Issue: 12

Published: April 18, 2023

The ubiquitin-binding NBR1 autophagy receptor plays a prominent role in recognizing ubiquitylated protein aggregates for vacuolar degradation by macroautophagy. Here, we show that upon exposing Arabidopsis plants to intense light, associates with photodamaged chloroplasts independently of ATG7, core component the canonical machinery. coats both surface and interior chloroplasts, which is then followed direct engulfment organelles into central vacuole via microautophagy-type process. relocalization does not require chloroplast translocon complexes embedded envelope but instead greatly enhanced removing self-oligomerization mPB1 domain NBR1. delivery NBR1-decorated vacuoles depends on UBA2 independent ubiquitin E3 ligases SP1 PUB4, known ubiquitylation proteins. Compared wild-type plants, nbr1 mutants have altered levels subset proteins display abnormal density sizes high light exposure. We postulate that, as lose integrity, cytosolic reach ubiquitylate thylakoid stroma are recognized autophagic clearance. This study uncovers new function damaged microautophagy.

Language: Английский

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Subcellular compartmentalization in the biosynthesis and engineering of plant natural products

Biotechnology Advances, Journal Year: 2023, Volume and Issue: 69, P. 108258 - 108258

Published: Sept. 16, 2023

Language: Английский

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Molecular priming with H2O2 and proline triggers antioxidant enzyme signals in maize seedlings during drought stress

Biochimica et Biophysica Acta (BBA) - General Subjects, Journal Year: 2024, Volume and Issue: 1868(7), P. 130633 - 130633

Published: May 16, 2024

Language: Английский

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Deciphering the functional roles of transporter proteins in subcellular metal transportation of plants

Planta, Journal Year: 2023, Volume and Issue: 258(1)

Published: June 14, 2023

Language: Английский

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Nitrogen‐mediated volatilisation of defensive metabolites in tomato confers resistance to herbivores

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(8), P. 3227 - 3240

Published: May 13, 2024

Abstract Plants synthesise a vast array of volatile organic compounds (VOCs), which serve as chemical defence and communication agents in their interactions with insect herbivores. Although nitrogen (N) is critical resource the production plant metabolites, its regulatory effects on defensive VOCs remain largely unknown. Here, we investigated effect N content tomato ( Solanum lycopersicum ) tobacco cutworm Spodoptera litura ), notorious agricultural pest, using biochemical molecular experiments combination behavioural performance analyses. We observed that leaves different contents, S. showed distinct feeding preference growth developmental performance. Particularly, metabolomics profiling revealed limited availability conferred resistance upon plants to likely associated biosynthesis emission metabolite α‐humulene repellent. Moreover, exogenous application elicited significant repellent response against Thus, our findings unravel key factors involved N‐mediated herbivores pave way for innovation management improve responses facilitate pest control strategies within agroecosystems.

Language: Английский

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Bacteria–organelle communication in physiology and disease

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(7)

Published: May 15, 2024

Bacteria, omnipresent in our environment and coexisting within body, exert dual beneficial pathogenic influences. These microorganisms engage intricate interactions with the human impacting both health disease. Simultaneously, certain organelles cells share an evolutionary relationship bacteria, particularly mitochondria, best known for their energy production role dynamic interaction each other organelles. In recent years, communication between bacteria mitochondria has emerged as a new mechanism regulating host's physiology pathology. this review, we delve into communications host shedding light on collaborative regulation of immune response, metabolism, aging, longevity. Additionally, discuss bacterial organelles, including chloroplasts, lysosomes, endoplasmic reticulum (ER).

Language: Английский

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Enhanced stress resilience in potato by deletion of Parakletos

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: June 18, 2024

Abstract Continued climate change impose multiple stressors on crops, including pathogens, salt, and drought, severely impacting agricultural productivity. Innovative solutions are necessary to develop resilient crops. Here, using quantitative potato proteomics, we identify Parakletos, a thylakoid protein that contributes disease susceptibility. We show knockout or silencing of Parakletos enhances resistance oomycete, fungi, bacteria, whereas its overexpression reduces resistance. In response biotic stimuli, -overexpressing plants exhibit reduced amplitude reactive oxygen species Ca 2+ signalling, does the opposite. homologues have been identified in all major Consecutive years field trials demonstrate deletion Phytophthora infestans increases yield. These findings demark susceptibility gene, which can be exploited enhance crop resilience towards abiotic stresses low-input agriculture.

Language: Английский

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