Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 19, 2023

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

The autophagy receptor NBR1 directs the clearance of photodamaged chloroplasts DOI Creative Commons
Han Nim Lee, Jenu V. Chacko, Ariadna González‐Solís

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: April 18, 2023

The ubiquitin-binding NBR1 autophagy receptor plays a prominent role in recognizing ubiquitylated protein aggregates for vacuolar degradation by macroautophagy. Here, we show that upon exposing Arabidopsis plants to intense light, associates with photodamaged chloroplasts independently of ATG7, core component the canonical machinery. coats both surface and interior chloroplasts, which is then followed direct engulfment organelles into central vacuole via microautophagy-type process. relocalization does not require chloroplast translocon complexes embedded envelope but instead greatly enhanced removing self-oligomerization mPB1 domain NBR1. delivery NBR1-decorated vacuoles depends on UBA2 independent ubiquitin E3 ligases SP1 PUB4, known ubiquitylation proteins. Compared wild-type plants, nbr1 mutants have altered levels subset proteins display abnormal density sizes high light exposure. We postulate that, as lose integrity, cytosolic reach ubiquitylate thylakoid stroma are recognized autophagic clearance. This study uncovers new function damaged microautophagy.

Language: Английский

Citations

23
Vacuolar Degradation of Plant Organelles DOI
Marisa S. Otegui, Charlotte Steelheart, Wenlong Ma

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3036 - 3056

Published: April 24, 2024

Plants continuously remodel and degrade their organelles due to damage from metabolic activities environmental stressors, as well an integral part of cell differentiation programs. Whereas certain use local hydrolytic enzymes for limited remodeling, most the pathways that control partial or complete dismantling rely on vacuolar degradation. Specifically, selective autophagic play a crucial role in recognizing sorting plant organelle cargo clearance, especially under cellular stress conditions induced by factors like heat, drought, damaging light. In these short reviews, we discuss mechanisms degradation chloroplasts, mitochondria, endoplasmic reticulum, Golgi, peroxisomes, with emphasis autophagy, recently discovered autophagy receptors organelles, crosstalk other catabolic pathways.

Language: Английский

Citations

7
Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants DOI

Erika Isono,

Jianming Li, Pablo Pulido

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3074 - 3098

Published: May 3, 2024

Proteome composition is dynamic and influenced by many internal external cues, including developmental signals, light availability, or environmental stresses. Protein degradation, in synergy with protein biosynthesis, allows cells to respond various stimuli adapt reshaping the proteome. degradation mediates final irreversible disassembly of proteins, which important for quality control eliminate misfolded damaged as well entire organelles. Consequently, it contributes cell resilience buffering against organellar damage caused Moreover, plays roles signaling, transcriptional translational events. The intricate task recognizing specific proteins achieved specialized systems that are tailored substrate's physicochemical properties subcellular localization. These recognize diverse substrate cues collectively referred "degrons," can assume a range configurations. They molecular surfaces recognized E3 ligases ubiquitin-proteasome system but also be considered general features other systems, autophagy even proteases. Here we provide an overview newest developments field, delving into processes recognition elucidating pathways through they recruited degradation.

Language: Английский

Citations

7
Autophagosome biogenesis and organelle homeostasis in plant cells DOI

Xiaohong Zhuang,

Baiying Li, Liwen Jiang

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3009 - 3024

Published: March 27, 2024

Autophagy is one of the major highly inducible degradation processes in response to plant developmental and environmental signals. In different stimuli, cellular materials, including proteins organelles, can be sequestered into a double membrane autophagosome structure either selectively or nonselectively. The formation an as well its delivery vacuole involves complex dynamic processes. identification characterization conserved autophagy-related (ATG) their related regulators have greatly advanced our understanding molecular mechanism underlying biogenesis function cells. Autophagosome tightly regulated by coordination multiple ATG non-ATG selective cargo recruitment. This review updates current knowledge biogenesis, with special emphasis on core machinery that drives autophagosome-organelle interactions under abiotic stress conditions.

Language: Английский

Citations

5
Recent advances in cellular degradation and nuclear control of leaf senescence DOI
Lei Pei, Fei Yu, Xiayan Liu

et al.

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(18), P. 5472 - 5486

Published: July 15, 2023

Abstract Senescence is the final stage of plant growth and development, a highly regulated process at molecular, cellular, organismal levels. When triggered by age, hormonal, or environmental cues, plants actively adjust their metabolism gene expression to execute progression senescence. Regulation senescence vital for reallocation nutrients sink organs, ensure reproductive success adaptations stresses. Identification characterization hallmarks leaf are great importance understanding molecular regulatory mechanisms senescence, breeding future crops with more desirable traits. Tremendous progress has been made in elucidating genetic network underpinning metabolic cellular changes In this review, we focus on three – chlorophyll chloroplast degradation, loss proteostasis, activation senescence-associated genes (SAGs), discuss recent findings players crosstalk pathways.

Language: Английский

Citations

13
The interplay between autophagy and chloroplast vesiculation pathways under dark‐induced senescence DOI Open Access
Jessica A. S. Barros, João Henrique F. Cavalcanti, Karla Pimentel

et al.

Plant Cell & Environment, Journal Year: 2023, Volume and Issue: 46(12), P. 3721 - 3736

Published: Aug. 24, 2023

In cellular circumstances where carbohydrates are scarce, plants can use alternative substrates for energetic maintenance. plants, the main protein reserve is present in chloroplast, which contains most of total leaf proteins and represents a rich source nitrogen amino acids. Autophagy plays key role chloroplast breakdown, well-recognised symptom both natural stress-induced plant senescence. Remarkably, an autophagic-independent route degradation associated with vesiculation (CV) gene was previously demonstrated. During extended darkness, CV highly induced absence autophagy, contributing to early senescence phenotype atg mutants. To further investigate under dark-induced conditions, mutants low expression (amircv) double amircv1xatg5 were characterised. Following darkness treatment, no aberrant phenotypes observed amircv single mutants; however, displayed altered dismantling membrane structures these conditions. Metabolic characterisation revealed that functional lack autophagy leads higher impairment acid release differential organic accumulation during starvation The data obtained discussed context terms metabolism regulation degradation.

Language: Английский

Citations

12
Does the polyubiquitination pathway operate inside intact chloroplasts to remove proteins? DOI Creative Commons
Klaas J. van Wijk, Zach Adam

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 2984 - 2989

Published: April 29, 2024

Language: Английский

Citations

4
Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC DOI Creative Commons
Ting Pan,

Yangxuan Liu,

Xufan Hu

et al.

Cell Reports, Journal Year: 2023, Volume and Issue: 42(10), P. 113208 - 113208

Published: Oct. 1, 2023

Clathrin-mediated vesicular formation and trafficking are responsible for molecular cargo transport signal transduction among organelles. Our previous study shows that CHLOROPLAST VESICULATION (CV)-containing vesicles (CVVs) generated from chloroplasts chloroplast degradation under abiotic stress. Here, we show CV interacts with the clathrin heavy chain (CHC) induces vesicle budding toward cytosol inner envelope membrane. In defective mutants of CHC2 dynamin-encoding DRP1A, CVV releasing impeded. The mutations inhibit CV-induced hypersensitivity to water Moreover, CV-CHC2 interaction is impaired by oxidized GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPC). GAPC1 overexpression suppresses CV-mediated stress, while silencing alleviates gapc1gapc2 plant Together, our work identifies a pathway clathrin-assisted outward chloroplast, which involved in stress response.

Language: Английский

Citations

11
Possible role of autophagy in microbial volatile pollutant-induced starch degradation and expression of hypoxia responsive genes DOI

Chung-Chih Huang,

Ching‐Han Chang,

Tu-Trinh Thi Truong

et al.

Environmental Pollution, Journal Year: 2025, Volume and Issue: 367, P. 125663 - 125663

Published: Jan. 9, 2025

Language: Английский

Citations

0
Multifaceted roles of the ATG8 protein family in plant autophagy: from autophagosome biogenesis to cargo recognition DOI Creative Commons
Yixin Wu, Rui Xu,

Xiaohong Zhuang

et al.

Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 168981 - 168981

Published: Feb. 1, 2025

Language: Английский

Citations

0