Development of in vitro model of exosome transport in microfluidic gut-brain axis-on-a-chip

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(19), P. 4581 - 4593

Published: Jan. 1, 2024

This study introduces a microfluidic gut–brain axis-on-a-chip model that simulates exosome delivery from the gut to brain using convective flow. Integrating real-time imaging with simulations, it advances research.

Language: Английский

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Insights into the unique roles of extracellular vesicles for gut health modulation: Mechanisms, challenges, and perspectives

Current Research in Microbial Sciences, Journal Year: 2024, Volume and Issue: 7, P. 100301 - 100301

Published: Jan. 1, 2024

Language: Английский

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Development of a dual-flow tissue perfusion device for modeling the gastrointestinal tract–brain axis

Biomicrofluidics, Journal Year: 2023, Volume and Issue: 17(5)

Published: Sept. 1, 2023

Despite the large number of microfluidic devices that have been described over past decade for study tissues and organs, few become widely adopted. There are many reasons this lack adoption, primarily constructed a single purpose or because they highly complex require relatively expensive investment in facilities training. Here, we describe microphysiological system (MPS) is simple to use provides fluid channels above below cells, tissue biopsies, maintained on disposable, poly(methyl methacrylate), carrier held between polycarbonate outer plates. All other fittings standard Luer sizes ease adoption. The can be coated with cells both sides generate membrane barriers, established series allow medium flow from one cell layer another. Furthermore, containing easily removed after treatment device visualized recovered additional off-chip analysis. A 0.4 μm monolayers proved most effective maintaining separate flows, allowing apical basal surfaces perfused independently. panel different lines (Caco-2, HT29-MTX-E12, SH-SY5Y, HUVEC) were successfully MPS up 7 days, either alone connected series. presence tight junctions mucin was expressed as expected by Caco-2 HT-29-MTX-E12, Concanavalin showing uniform staining. Addition Annexin V PI showed viability these >80% at days. Bacterial extracellular vesicles (BEVs) produced Bacteroides thetaiotaomicron labeled 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbo-cyanine perchlorate (DiD) used model component human colonic microbiota translocating an surface differentiated SH-SY5Y neuronal cultured devices. newly adapted, changing maintain spheroids, pieces, slices biopsy joined variety processes. layers made more through addition multiple types and/or patterning matrix ability culture adjacent another cell:cell transfer, e.g., passive active drug virus bacterial entry BEV uptake transfer.

Language: Английский

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Extracellular vesicles: Function, resilience, biomarker, bioengineering, and clinical implications

Tzu Chi Medical Journal, Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Extracellular vesicles (EVs) have emerged as key players in intercellular communication, disease pathology, and therapeutic innovation. Initially overlooked cellular debris, EVs are now recognized vital mediators of cell-to-cell ferrying a cargo proteins, nucleic acids, lipids, providing resilience response to stresses. This review provides comprehensive overview EVs, focusing on their role biomarkers diagnosis, functional significance physiological pathological processes, the potential bioengineering for applications. offer promising avenue noninvasive diagnosis monitoring, reflecting state originating cells. Their diagnostic spans spectrum diseases, including cancer, cardiovascular disorders, neurodegenerative infectious diseases. Moreover, presence bodily fluids such blood, urine, cerebrospinal fluid enhances utility, presenting advantages over traditional methods. Beyond diagnostics, mediate crucial roles facilitating transfer bioactive molecules between communication modulates various processes tissue regeneration, immune modulation, neuronal communication. Dysregulation EV-mediated is implicated diseases highlighting potential. Bioengineering techniques avenues manipulating applications, from isolation purification engineering targeted delivery systems. These approaches hold promise developing novel therapeutics tailored specific revolutionizing personalized medicine. However, challenges standardization, scalability, regulatory approval need addressing successful clinical translation. Overall, represent dynamic frontier biomedical research with vast therapeutics,

Language: Английский

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Emerging microfluidic gut-on-a-chip systems for drug development

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

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