Serological Biomarkers at Hospital Admission and Hospitalization Treatments Are Not Related to Sensitization-Associated Symptoms in Patients with Post-COVID Pain DOI Creative Commons
César Fernández‐de‐las‐Peñas, Carlos Guijarro, Juan Torres‐Macho

et al.

Pathogens, Journal Year: 2023, Volume and Issue: 12(10), P. 1235 - 1235

Published: Oct. 12, 2023

Current evidence suggests that a group of patients who had survived coronavirus disease, 2019 (COVID-19) and developed post-COVID pain can exhibit altered nociceptive processing. The role serological biomarkers hospitalization treatments in is unclear. This study aimed to investigate the association received during with sensitization-associated symptoms COVID-19 survivors pain. One hundred eighty-three (n = 183) been hospitalized due one urban hospital Madrid (Spain) first wave pandemic were assessed face-to-face interview 9.4 (SD 3.4) months after hospitalization. Levels 19 biomarkers, data, obtained from records. Sensitization-associated (Central Sensitization Inventory, CSI), sleep quality (Pittsburgh Sleep Quality Index, PSQI), catastrophism (Pain Catastrophizing Scale), anxiety/depressive level (Hospital Anxiety Depression Scale, HADS) assessed. prevalence was 40.9% 75). Twenty-nine (38.6%) symptoms. Overall, no differences data identified according presence analysis revealed exhibited higher lymphocyte count lower urea levels than those without symptoms, but small. Pain depressive levels, not fatigue, dyspnea, brain fog, anxiety or poor sleep, individuals In conclusion, this are associated at admission received.

Language: Английский

Fecal bacteria transplantation replicates aerobic exercise to reshape the gut microbiota in mice to inhibit high-fat diet-induced atherosclerosis DOI Creative Commons

Jie Men,

Hao Li,

Chenbin Cui

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0314698 - e0314698

Published: Feb. 4, 2025

Aerobic exercise exerts a significant impact on the gut microbiota imbalance and atherosclerosis induced by high-fat diet. However, whether fecal transplantation, based aerobic exercise, can improve progression remains unexplored. In this study, we utilized male C57 mice to establish models of atherosclerosis, followed transplantation(Fig 1a). Firstly, analyzed body weight, somatotype, adipocyte area, aortic HE images model mice. Our findings revealed that diet -induced exhibited elevated lipid accumulation, larger more severe progression. Additionally, assessed plasma levels, inflammatory factors, composition in each group displayed dyslipidemia along with responses reduced diversity as well abundance beneficial bacteria. Subsequently performing transplantation demonstrated experienced weight loss accompanied accumulation while normalizing their profile; furthermore it significantly improved blood lipids inflammation markers thereby exhibiting notable anti- effects. The suggest modify diet-induced atherosclerosis(Fig 1b). Moreover, these effects be effectively transmitted through offering promising therapeutic approach for managing atherosclerosis.

Language: Английский

Citations

1
The Role of Mitochondria in Statin-Induced Myopathy DOI

G.M. Bell,

Anastasia Thoma,

Iain P. Hargreaves

et al.

Drug Safety, Journal Year: 2024, Volume and Issue: 47(7), P. 643 - 653

Published: March 16, 2024

Language: Английский

Citations

7
Idebenone ameliorates statin-induced myotoxicity in atherosclerotic ApoE−/− mice by reducing oxidative stress and improving mitochondrial function DOI

Wenfei Yu,

Wenjing Wu,

Dandan Zhao

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(5), P. 167157 - 167157

Published: April 4, 2024

Language: Английский

Citations

5
PGC1-α-driven mitochondrial biogenesis contributes to a cancer stem cell phenotype in melanoma DOI Creative Commons
Fabrizio Fontana, Chiara Macchi, Martina Anselmi

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2023, Volume and Issue: 1870(1), P. 166897 - 166897

Published: Sept. 25, 2023

Little is known about the metabolic regulation of cancer stem cells (CSCs) in melanoma. Here, we used A375 and WM115 cell lines to dissect role mitochondria conferring CSC traits. Notably, observed that melanospheres, be enriched ABCG2+ CSCs, showed higher mitochondrial mass compared with their adherent counterpart. In particular, they displayed increased PGC1-α expression oxidative phosphorylation (OXPHOS) complex levels, leading a switch characterized by enhanced membrane potential, oxygen consumption, ATP synthesis ROS production. Interestingly, silencing resulted suppression features, including clonogenic ability, migration, spheroid formation ABCG2 enrichment. Similarly, XCT790 SR-18292, two inhibitors, were able not only reduce melanoma tumorigenicity invasion but also block melanosphere growth propagation proliferation. conclusion, improved biogenesis associated stem-like phenotype melanoma, therapeutically targeting mitochondria-enriched subpopulation might overcome tumor progression.

Language: Английский

Citations

10
Integrated metabolomics and network pharmacology analysis to reveal the mechanisms of naringin against atherosclerosis DOI

Gaoning Zhang,

Xiaoyi Yin, Xiao Tang

et al.

Journal of Pharmacy and Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Objectives The purpose of this study was to explore the mechanism naringin in atherosclerotic mice from perspective network pharmacology and non-targeted metabolomics. Methods ApoE−/− were induced establish an model pharmacodynamics potential atherosclerosis (AS). Pathological section blood lipid levels used evaluate intervention effects. core targets, metabolites, related pathways alleviating predicted through metabolomics analysis. Furthermore, inflammatory factors pathway-related protein expression detected using ELISA Western blot methods. Key findings It turned out that compared with group, could reduce development degree mice. suggested PI3K-AKT pathway important for interfere AS. Serum metabolic data collected analyzed, a total 27 biomarkers identified, involving vitamin B6 metabolism, arginine retinol metabolism. experiment verified inhibited inflammation AS PI3K-AKT/TLR4/NF-κB pathway. Conclusions This provides strategy combining alleviation by offers new idea its application.

Language: Английский

Citations

0
PCSK9 Confers Inflammatory Properties to Extracellular Vesicles Released by Vascular Smooth Muscle Cells DOI Open Access
Maria Francesca Greco, Alessandra Stefania Rizzuto, M Zara

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 13065 - 13065

Published: Oct. 28, 2022

Vascular smooth muscle cells (VSMCs) are key participants in both early- and late-stage atherosclerosis influence neighbouring possibly by means of bioactive molecules, some which packed into extracellular vesicles (EVs). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is expressed secreted VSMCs. This study aimed to unravel the role PCSK9 on VSMCs-derived EVs terms content functionality. were isolated from human VSMCs overexpressing (VSMCPCSK9-EVs) tested endothelial cells, monocytes, macrophages a model zebrafish embryos. Compared released wild-type VSMCs, VSMCPCSK9-EVs caused rise expression adhesion molecules pro-inflammatory cytokines monocytes. These acquired an increased migratory capacity, reduced oxidative phosphorylation proteins involved immune response effector processes. Concerning macrophages, enhanced inflammatory milieu uptake oxidized low-density lipoproteins, whereas capacity was reduced. When injected embryos, favoured recruitment toward site injection. The results present provide evidence that plays EVs, at least those derived vascular origin.

Language: Английский

Citations

15
Low-dose atorvastatin protects skeletal muscle mitochondria in high-fat diet-fed mice with mitochondrial autophagy inhibition and fusion enhancement DOI Creative Commons
Peng Zheng, Qian Zhang, Wenjing Ma

et al.

European Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 959, P. 176085 - 176085

Published: Oct. 6, 2023

Despite the great clinical benefits of statins in cardiovascular diseases, their widespread use may lead to adverse muscle reactions associated with mitochondrial dysfunction. Some studies have demonstrated that provide substantial improvement skeletal health mice. Our previous study found oral treatment atorvastatin (Ator, 3 mg/kg) protected myocardial mitochondria high-fat diet (HFD)-fed Therefore, this aimed explore influence low-dose Ator (3 on under cholesterol overload. Male C57BL/6J mice were fed a HFD for 18 weeks and orally administered during last 12 weeks. had no effects elevated serum glucose levels HFD-fed Serum creatine kinase cross-sectional area cells not affected by feeding or treatment. Increased expression PINK1-LC3 II (activated mitophagy), MFN2 (fusion), PGC-1α (biogenesis) proteins was induced muscles Treatment inhibited PINK1 LC3 protein expression, but further promoted MFN1, MFN2, OPA1 expression. The impairments quality morphology attenuated Ator. Furthermore, enhanced oxidation capacity restored ATP production reveals has protective effect mice, likely through inhibiting mitophagy enhancing fusion. This suggests be one Ator-mediated targets.

Language: Английский

Citations

5
Lentilactobacillus kefiri SGL 13 and Andrographis paniculata alleviate dextran sulfate sodium induced colitis in mice DOI Creative Commons

Laura Manna,

Eleonora Rizzi,

Eleonora Bafile

et al.

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: Feb. 13, 2023

Inflammatory bowel diseases (IBD) are chronic inflammatory conditions that typically involve diarrhea, abdominal pain, fatigue, and weight loss, with a dramatic impact on patients' quality of life. Standard medications often associated adverse side effects. Thus, alternative treatments such as probiotics great interest. The purpose the present study was to evaluate effects oral administration Lentilactobacillus kefiri (basonym: Lactobacillus kefiri) SGL 13 Andrographis paniculata, namely, Paniculin 13™, dextran sodium sulfate (DSS)- treated C57BL/6J mice.Colitis induced by administering 1.5% DSS in drinking water for 9 days. Forty male mice were divided into four groups, receiving PBS (control), DSS, 13™ + 13™.The results showed body loss Disease Activity Index (DAI) score improved 13™. Moreover, ameliorated DSS-induced dysbiosis, modulating gut microbiota composition. gene expression MPO, TNFα iNOS colon tissue reduced these data matched histological results, supporting efficacy reducing response. No administration.In conclusion, could be an effective add-on approach conventional therapies IBD.

Language: Английский

Citations

4
Pharmacotherapy of the Lipid-Lowering Drugs: Update on Efficacy and Risk DOI Open Access
Sabata Pierno, Olimpia Musumeci

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 996 - 996

Published: Jan. 4, 2023

Lipid-lowering drugs are widely used for the prevention and cure of cardiovascular diseases (CVD) [...].

Language: Английский

Citations

4
Mechanism of static loading injury in human skeletal muscle cells DOI
Yuan Yuan, Hui Zhang, Yafang Li

et al.

American Journal of Translational Research, Journal Year: 2024, Volume and Issue: 16(4), P. 1135 - 1144

Published: Jan. 1, 2024

To establish a cellular-level mechanical injury model for human skeletal muscle cells and investigate changes in the effect mechanism after such injuries.

Language: Английский

Citations

1