Neuronal and astrocyte NCX isoform/splice variants: How do they participate in Na+ and Ca2+ signalling?

Cell Calcium, Journal Year: 2023, Volume and Issue: 116, P. 102818 - 102818

Published: Oct. 24, 2023

Language: Английский

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Adiponectin Alleviates Cell Injury due to Cerebrospinal Fluid from Multiple Sclerosis Patients by Inhibiting Oxidative Stress and Proinflammatory Response

Biomedicines, Journal Year: 2023, Volume and Issue: 11(6), P. 1692 - 1692

Published: June 12, 2023

Multiple sclerosis (MS) is the most common disabling neurological disease characterized by chronic inflammation and neuronal cell viability impairment. Based on previous studies reporting that adiponectin exhibits neuroprotective effects in some models of neurodegenerative diseases, we analyzed AdipoRon treatment, alone or combination with cerebrospinal fluid patients MS (MS-CSF), to verify whether this adipokine acts basal cellular processes. To aim, SH-SY5Y U-87 cells (models glial cells, respectively) were exposed MS-CSF co-treatment AdipoRon. The was determined via MTT assay, possible underlying mechanisms investigated alterations oxidative stress inflammation. assay confirmed did not affect both lines; whereas, when used MS-CSF, it reduces inhibitory lines. In addition, treatment causes an increase pro-inflammatory cytokines, whereas determines reduction anti-inflammatory IL-10. Interestingly, co-administration counteracts MS-CSF-induced production enhancement conclusion, our data suggest cytotoxic induced lines one potential molecular might occur Further vivo vitro are essential confirm could be a neuro-protectant candidate against injury.

Language: Английский

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Improvement effects of green tea and pumpkin oils on myelin oligodendrocyte glycoprotein-induced Multiple sclerosis in rats

Journal of Functional Foods, Journal Year: 2023, Volume and Issue: 111, P. 105876 - 105876

Published: Nov. 10, 2023

Multiple sclerosis (MS) is a demyelinating disorder of the central nervous system (CNS) associated with significant progressive neurodegeneration. There lot interest in use plant-based essential oils traditional medicine to treat and prevent human illnesses, including MS. This research aimed assess neuroprotective effects green tea oil (GTO) pumpkin (PO) against myelin oligodendrocyte glycoprotein (MOG)-induced MS Wistar rats as well investigate underlying molecular mechanisms. The were divided into four groups: group 1, normal control; 2, MOG-injected; groups 3 4, MOG-injected treated 5 ml/kg body weight each GTO PO, respectively. chemical profiles components within PO identified using gas chromatography-mass spectrometry (GC–MS). Treatment substantially improved decreased dopamine, serotonin, norepinephrine, acetylcholine levels brain rats. It also suppressed elevated epinephrine levels. histological injuries cortical tissue notably after supplementing PO. Furthermore, lipid peroxidation serum INF-β concentration significantly lower GSH, SOD, GPx, coenzyme Q10, α-tocopherol enhanced by supplementaion. Additionally, administration upregulated Nrf2, Bcl-2, PCNA while downregulated TNF-α, NF-κB, iNOS, p53, Bax expression Taken together, these findings suggest that efficiently ameliorate MOG-induced via enhancing antioxidant, anti-inflammatory, anti-apoptotic effects.

Language: Английский

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Comparing Single-Cell Transcriptomes of Blood and Cerebrospinal Fluid Leukocytes in Multiple Sclerosis

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 9, 2024

Abstract Background Multiple sclerosis (MS) is a chronic autoimmune disorder of the central nervous system, marked by inflammation, demyelination, and neurodegeneration. Diagnosis complex due to overlapping symptoms with other neurological conditions, typically relying on clinical evaluation, exams, tests like magnetic resonance imaging (MRI) cerebrospinal fluid (CSF) analysis. Recent advances in technology, particularly single-cell analysis blood CSF leukocytes, hold promise for enhancing MS diagnosis providing insights into immune cell involvement at molecular level, potentially enabling more precise diagnostics personalized treatments. Method We acquired RNA Sequence (RNA-Seq) data ( GSE138266 ) from website National Institutes Health United States (NIH), comprising samples patients diagnosed idiopathic intracranial hypertension (IIH) MS. Our focused identifying genes, pathways gene ontology terms distinct expression patterns compared IIH. Results identified clear differences profiles between MS, contrasting leukocyte The increased genes suggests boost activity regulation cellular proliferation, while decreased points disruptions across various functional categories. Gene identifies upregulated associated differentiation, apoptotic regulation, responses downregulated suggest signaling cascades myelination processes. Similarly, Reactome pathway unveils related cycle mechanisms, indicative oxygen transport metabolism. Conclusion study offers thorough examination transcriptomic data, unveiling unique patterns, terms, linked pathophysiology. Notably, our findings identify CD69 HNRNPK as potential key driving progression. By clarifying IIH, enhances grasp pathogenesis promising targets diagnostic therapeutic interventions.

Language: Английский

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Inflammation and neurodegeneration in multiple sclerosis

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 321 - 345

Published: Jan. 1, 2024

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