Cited by Preserving Blood–Brain Barrier Integrity in Ischemic Stroke: a Review on MSCs-sEVs Content and Potential Molecular Targets

Exosomes in brain diseases: Pathogenesis and therapeutic targets DOI

Qingying Si, Linlin Wu,

Deshui Pang

et al.

MedComm, Journal Year: 2023, Volume and Issue: 4(3)

Published: June 1, 2023

Exosomes are extracellular vesicles with diameters of about 100 nm that naturally secreted by cells into body fluids. They derived from endosomes and wrapped in lipid membranes. involved intracellular metabolism intercellular communication. contain nucleic acids, proteins, lipids, metabolites the cell microenvironment cytoplasm. The contents exosomes can reflect their cells' origin allow observation tissue changes states under disease conditions. Naturally have specific biomolecules act as "fingerprint" parent cells, changed pathological conditions be used biomarkers for diagnosis. low immunogenicity, small size, cross blood-brain barrier. These characteristics make unique engineering carriers. incorporate therapeutic drugs achieve targeted drug delivery. carriers therapy still infancy, but exosome provides a new perspective cell-free therapy. This review discussed relationship occurrence treatment some neuropsychiatric diseases. In addition, future applications diagnosis disorders were evaluated this review.

Language: Английский

Citations

Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis DOI

Qiongqiong Zhang,

Peiying Deng,

Suhui Chen

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 20, 2023

Electroacupuncture (EA) and induced pluripotent stem cell (iPSC)-derived small extracellular vesicles (iPSC-EVs) have substantial beneficial effects on ischemic stroke. However, the detailed mechanisms remain unclear. Here, we explored underlying regulation of EA iPSC-EVs in microbiome-gut-brain axis (MGBA) after Ischemic stroke mice (C57BL/6) were subjected to middle cerebral artery occlusion (MCAO) or Sham surgery. treatments significantly improved neurological function neuronal intestinal tract injury, downregulated levels IL-17 expression upregulated IL-10 brain colon tissue ischemia−reperfusion. also modulated microbiota composition diversity as well differential distribution species intestines Our results demonstrated that regulated immunity through MGBA microbes, reducing damage following ischemia positively impacting outcomes findings provide new insights into application combined with a treatment for

Language: Английский

Citations

Exosome-like nanoparticles derived from fruits, vegetables, and herbs: innovative strategies of therapeutic and drug delivery DOI

Bo Zhao,

Hangjuan Lin,

Xinchi Jiang

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(12), P. 4598 - 4621

Published: Jan. 1, 2024

Over the past ten years, significant advancements have been made in exploring plant-derived exosome-like nanoparticles (PELNs) for disease therapeutics and drug delivery. PELNs, as inherent nanoscale particles comprised of proteins, lipids, nucleic acids, secondary metabolites, exhibit capacity cellular uptake by human cells. This intercellular interaction transcends biological boundaries, effectively influencing functions animals. PELNs outstanding biocompatibility, low immunogenicity, enhanced safety, environmentally friendly sustainability. article summarized preparation methods characteristics PELNs. It provided a systematic review varied roles derived from fruits, vegetables, herbs The challenges their production application were discussed, future prospects this rapidly evolving field explored.

Language: Английский

Citations

M1-type microglia-derived exosomes contribute to blood–brain barrier damage DOI

Wen Jiang,

Yan Wu,

Ailan Pang

et al.

Brain Research, Journal Year: 2024, Volume and Issue: 1835, P. 148919 - 148919

Published: April 6, 2024

Language: Английский

Citations

BMSCs-derived small extracellular vesicles antagonize cerebral endothelial Caveolin-1 driven autophagic degradation of tight-junction proteins to protect blood-brain barrier post-stroke DOI

Yiyang Li,

Xingping Quan,

Jinxia Hu

et al.

International Journal of Biological Sciences, Journal Year: 2025, Volume and Issue: 21(2), P. 842 - 859

Published: Jan. 1, 2025

Bone marrow mesenchymal stem cells (BMSCs) -derived extracellular vesicles (EVs), especially small EVs (sEVs), were vastly reported to enable multiple restorative effects on ischemic stroke, yet the protective mechanism of blood-brain barrier (BBB) has not been fully illustrated.In present study, we investigated therapeutic and BMSCs-derived sEVs BBB injury after stroke.In-vivo, administering transient middle cerebral artery occlusion (tMCAo) mice mitigated brain infarct volume, permeability neural apoptosis, improved blood flow perfusion neurological function.Simultaneously, vascular endothelial overexpressed Caveolin-1 (Cav-1) together with its strong co-localization autophagosome protein LC3B suppressed, ZO-1 Occludin expressions enhanced, whose results consistent those oxygen-glucose-deprivation/reperfusion (OGD/R)-insulted (BECs) in vitro.Furthermore, by employing Cav-1 siRNA pcDNA3.1 transfection, Co-immunoprecipitation, cycloheximide assay, molecular docking, it proved that was an essential upstream autophagy activation, contributing tight-junction proteins delegation via autophagy-lysosomal pathway.Altogether, our study demonstrates novel Cav-1-dependent autophagic disruption integrity BMSC-sEVs treatment can reverse such hazard cascades.

Language: Английский

Citations

Engineered exosomes derived from stem cells: a new brain-targeted strategy DOI

Ahmed Waqas,

Songze Huang,

Lukui Chen

et al.

Expert Opinion on Drug Delivery, Journal Year: 2024, Volume and Issue: 21(1), P. 91 - 110

Published: Jan. 2, 2024

Using engineered exosomes produced from stem cells is an experimental therapeutic approach for treating brain diseases. According to reports, preclinical research has demonstrated notable neurogenesis and angiogenesis effects using modified cell-derived exosomes. These biological nanoparticles have a variety of anti-apoptotic, anti-inflammatory, antioxidant properties that make them very promising nervous system disorders.

Language: Английский

Citations

Human-induced pluripotent stem cell–derived neural stem cell exosomes improve blood–brain barrier function after intracerebral hemorrhage by activating astrocytes via PI3K/AKT/MCP-1 axis DOI

Conglin Wang, Fangyuan Cheng,

Zhaoli Han

et al.

Neural Regeneration Research, Journal Year: 2024, Volume and Issue: 20(2), P. 518 - 532

Published: April 16, 2024

JOURNAL/nrgr/04.03/01300535-202502000-00029/figure1/v/2024-05-28T214302Z/r/image-tiff Cerebral edema caused by blood-brain barrier injury after intracerebral hemorrhage is an important factor leading to poor prognosis. Human-induced pluripotent stem cell-derived neural cell exosomes (hiPSC-NSC-Exos) have shown potential for brain repair in central nervous system diseases. In this study, we explored the impact of hiPSC-NSC-Exos on preservation and underlying mechanism. Our results indicated that intranasal delivery mitigated neurological deficits, enhanced integrity, reduced leukocyte infiltration a mouse model hemorrhage. Additionally, decreased immune infiltration, activated astrocytes, secretion inflammatory cytokines like monocyte chemoattractant protein-1, macrophage protein-1α, tumor necrosis factor-α post-intracerebral hemorrhage, thereby improving microenvironment. RNA sequencing hiPSC-NSC-Exo PI3K/AKT signaling pathway astrocytes protein-1 secretion, integrity. Treatment with inhibitor LY294002 or neutralizing agent C1142 abolished these effects. summary, our findings suggest maintains part downregulating through activation astrocytes.

Language: Английский

Citations

Therapeutic gene delivery by mesenchymal stem cell for brain ischemia damage: Focus on molecular mechanisms in ischemic stroke DOI

Raed Obaid Saleh,

Ali A. Majeed,

Ria Margiana

et al.

Cell Biochemistry and Function, Journal Year: 2024, Volume and Issue: 42(2)

Published: March 1, 2024

Abstract Cerebral ischemic damage is prevalent and the second highest cause of death globally across patient populations; it as a substantial reason morbidity mortality. Mesenchymal stromal cells (MSCs) have garnered significant interest potential treatment for cerebral damage, shown in stroke, because their potent intrinsic features, which include self‐regeneration, immunomodulation, multi‐potency. Additionally, MSCs are easily obtained, isolated, cultured. Despite this, there number obstacles that hinder effectiveness MSC‐based treatment, such adverse microenvironmental conditions both vivo vitro. To overcome these obstacles, naïve MSC has undergone modification processes to enhance its innate therapeutic qualities. Genetic preconditioning (with medications, growth factors, other substances) two main categories into techniques can be separated. This field advanced significantly still attracting attention innovation. We examine cutting‐edge methods preserving even improving natural biological functions relation adhesion, migration, homing target site, survival, delayed premature senescence. address use genetically altered stroke‐induced damage. Future strategies result addressing difficulties associated with also discussed.

Language: Английский

Citations

Exosomes: The endogenous nanomaterials packed with potential for diagnosis and treatment of neurologic disorders DOI

Xuelin Tang, Yuxuan He,

Jinchi Liu

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2024, Volume and Issue: 239, P. 113938 - 113938

Published: May 3, 2024

Language: Английский

Citations

The dual role of microglia in intracerebral hemorrhage DOI

Yue Sun,

Wentao Sun,

Jiawei Liu

et al.

Behavioural Brain Research, Journal Year: 2024, Volume and Issue: 473, P. 115198 - 115198

Published: Aug. 10, 2024

Language: Английский

Citations