A Novel Biocidal Nanocomposite: Spherical Silica with Silver Ions Anchored at the Surface DOI Open Access
Magdalena Laskowska, Paweł Kowalczyk, Agnieszka Karczmarska

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 545 - 545

Published: Dec. 29, 2022

This article is devoted to a novel class of antimicrobial agents: nanocomposites composed spherical silica and silver ions located at the silica’s surface with assumed distribution. Such materials are in high demand due increasing threat from bacterial strains that becoming resistant currently known antibiotics. In particular, we focus on make it possible limit growth colonies variety tactile surfaces. this paper, present method for preparing silica-based nanocomposite containing analysis their properties. Our research revealed presence tested induces very oxidative stress bacteria cell, damaging modifying DNA, creating oxidized guanines, cytosines, or adenines, which causes its rapid destruction, leading cell death.

Language: Английский

Effect of the support alkyl chain nature in the functional properties of the immobilized lipases DOI
Diandra de Andrades, Pedro Abellanas-Pérez, Javier Rocha‐Martín

et al.

Enzyme and Microbial Technology, Journal Year: 2025, Volume and Issue: 184, P. 110583 - 110583

Published: Jan. 11, 2025

Language: Английский

Citations

3
A comprehensive review of lipase-catalyzed acidolysis as a method for producing structured glycerides DOI
Veymar G. Tacias-Pascacio, Pedro Abellanas-Pérez, Diandra de Andrades

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142878 - 142878

Published: April 1, 2025

Language: Английский

Citations

2
Enzyme loading in the support and medium composition during immobilization alter activity, specificity and stability of octyl agarose-immobilized Eversa Transform DOI Creative Commons
Guilherme J. Sabi,

Leonardo de Souza,

Pedro Abellanas-Pérez

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139667 - 139667

Published: Jan. 9, 2025

Language: Английский

Citations

1
The Effects of Buffer Nature on Immobilized Lipase Stability Depend on Enzyme Support Loading DOI Open Access
Pedro Abellanas-Pérez, Diego Carballares, Javier Rocha‐Martín

et al.

Catalysts, Journal Year: 2024, Volume and Issue: 14(2), P. 105 - 105

Published: Jan. 26, 2024

The lipases from Thermomyces lanuginosus (TLL) and Candida antarctica (B) (CALB) were immobilized on octyl-agarose beads at 1 mg/g (a loading under the capacity of support) by overloading support with enzymes. These biocatalysts compared in their stabilities 10 mM sodium phosphate, HEPES, Tris-HCl pH 7. Lowly loaded CALB was more stable than highly preparation, while TLL this effect smaller. Phosphate very negative for stability biocatalyst moderately using both loadings. enzymes HEPES presented a different response as function enzyme (e.g., lowly CALB, similar buffers, but it clearly smaller biocatalysts). Moreover, specific activity versus p-nitrophenol butyrate, triacetin R- or S-methyl mandelate depended buffer, loading, interaction between them. In some cases, almost twice expected could be obtained octyl-CALB, depending buffer. A co-interaction effects specificity buffer nature detected.

Language: Английский

Citations

5
One-Pot Synthesis of 1,3,5-Trisubstitued Pyrazoles via Immobilized Thermomyces lanuginosus Lipase (TLL) on a Metal–Organic Framework DOI Creative Commons
Zeynab Rangraz, Mostafa M. Amini, Zohreh Habibi

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(17), P. 19089 - 19098

Published: April 17, 2024

A regioselective enzyme-catalyzed system is selected for the synthesis of 1,3,5-trisubstituted pyrazole derivatives by adding phenyl hydrazines, nitroolefins, and benzaldehydes. The reaction performed in a one-pot vessel with yield ranging from 49 to 90%. TLL@MMI, immobilized Thermomyces lanuginosus lipase (TLL) on multivariate MOF-5/IRMOF-3 (MMI), showed good performance catalysis this reaction. prepared biocatalyst was characterized FTIR, XRD, SEM, EDX. thermal solvent stability TLL@MMI investigated MeOH EtOH after 24 h incubation. In presence 100% concentrations EtOH, has 80% activity.

Language: Английский

Citations

4
Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique DOI Creative Commons
Diána Balogh‐Weiser,

Alexandra Molnár,

Gergő Dániel Tóth

et al.

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(4), P. 1174 - 1174

Published: April 7, 2023

The application of enzyme-based therapies has received significant attention in modern drug development. Lipases are one the most versatile enzymes that can be used as therapeutic agents basic skin care and medical treatment related to excessive sebum production, acne, inflammation. traditional formulations available for treatment, such creams, ointments or gels, widely applied; however, their use is not always accompanied by good penetration properties, stability, patient adherence. Nanoformulated drugs offer possibility combining enzymatic small molecule formulations, making them a new exciting alternative this field. In study polymeric nanofibrous matrices made polyvinylpyrrolidone polylactic acid were developed, entrapping lipases from Candida rugosa Rizomucor miehei antibiotic compound nadifloxacin. effect type polymers investigated, nanofiber formation process was optimized provide promising topical treatment. Our experiments have shown entrapment electrospinning induced two orders magnitude increase specific enzyme activity lipases. Permeability investigations indicated all lipase-loaded masks capable delivering nadifloxacin human epidermis, confirming viability formulation method medications.

Language: Английский

Citations

9
Harnessing biocatalysis as a green tool in antibiotic synthesis and discovery DOI Creative Commons
Guilherme Fernandes, Seong‐Heun Kim, Daniele Castagnolo

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(41), P. 30396 - 30410

Published: Jan. 1, 2024

Biocatalysis offers a sustainable approach to drug synthesis, leveraging the high selectivity and efficiency of enzymes.

Language: Английский

Citations

1
Hen egg white lysozyme encapsulated in ZIF-8 for performing promiscuous enzymatic Mannich reaction DOI Creative Commons
Hamid R. Kalhor,

Zeinab Piraman,

Yasaman Fathali

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 107807 - 107807

Published: Sept. 1, 2023

Hen egg white lysozyme (HEWL) was exploited for the synthesis of β-amino carbonyl compounds through a direct and three-component Mannich reaction in aqueous, confirming high chemoselectivity toward imine. In order to further extend applications enzyme, HEWL encapsulated using metal-organic framework (MOF). The reactivity, stereoselectivity, reusability enzyme were investigated. significantly enhanced as compared non-encapsulated enzyme. A mutated version containing Asp52Ala (D52A), lacking important catalytical residue, has lost bacterial site activity against Micrococcus luteus (M. luteus) while D52A variant displayed an increased rate reaction, indicating different residue involved promiscuous reaction. Based on site-directed mutagenesis, molecular docking, dynamic studies, it proposed that π-stacking, H-bond interactions, presence water active may play crucial roles mechanism

Language: Английский

Citations

3
Lipase-Catalyzed Phospha-Michael Addition Reactions under Mild Conditions DOI Creative Commons

Yuelin Xu,

Fengxi Li, Jinglin Ma

et al.

Molecules, Journal Year: 2022, Volume and Issue: 27(22), P. 7798 - 7798

Published: Nov. 12, 2022

Organophosphorus compounds are the core structure of many active natural products. The synthesis these is generally achieved by metal catalysis requiring specifically functionalized substrates or harsh conditions. Herein, we disclose phospha-Michael addition reaction biphenyphosphine oxide with various substituted β-nitrostyrenes benzylidene malononitriles. This biocatalytic strategy provides a direct route for C-P bonds good functional group compatibility and simple practical operation. Under optimal conditions (styrene (0.5 mmol), Novozym 435 (300 U), EtOH (1 mL)), lipase leads to formation organophosphorus in yields up 94% at room temperature. Furthermore, confirm role catalytic triad this reaction. new system will have broad applications organic synthesis.

Language: Английский

Citations

5
A Novel Biocidal Nanocomposite: Spherical Silica with Silver Ions Anchored at the Surface DOI Open Access
Magdalena Laskowska, Paweł Kowalczyk, Agnieszka Karczmarska

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 24(1), P. 545 - 545

Published: Dec. 29, 2022

This article is devoted to a novel class of antimicrobial agents: nanocomposites composed spherical silica and silver ions located at the silica’s surface with assumed distribution. Such materials are in high demand due increasing threat from bacterial strains that becoming resistant currently known antibiotics. In particular, we focus on make it possible limit growth colonies variety tactile surfaces. this paper, present method for preparing silica-based nanocomposite containing analysis their properties. Our research revealed presence tested induces very oxidative stress bacteria cell, damaging modifying DNA, creating oxidized guanines, cytosines, or adenines, which causes its rapid destruction, leading cell death.

Language: Английский

Citations

2