Involvement of aquaporin 5 in Sjögren’s syndrome

Autoimmunity Reviews, Journal Year: 2023, Volume and Issue: 22(3), P. 103268 - 103268

Published: Jan. 5, 2023

Language: Английский

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Aquaporin-5 Dynamic Regulation

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 1889 - 1889

Published: Jan. 18, 2023

Aquaporin-5 (AQP5), belonging to the aquaporins (AQPs) family of transmembrane water channels, facilitates osmotically driven flux across biological membranes and movement hydrogen peroxide CO2. Various mechanisms have been shown dynamically regulate AQP5 expression, trafficking, function. Besides fulfilling its primary permeability function, has downstream effectors playing roles in various cellular processes. This review provides a comprehensive overview current knowledge upstream gain an in-depth understanding physiological pathophysiological processes involving AQP5.

Language: Английский

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Aquaporin regulates cell rounding through vacuole formation during endothelial-to-hematopoietic transition

Development, Journal Year: 2023, Volume and Issue: 150(11)

Published: June 1, 2023

ABSTRACT Endothelial-to-hematopoietic transition (EHT) is crucial for hematopoietic stem cell (HSC) generation. During EHT, the morphology of hemogenic endothelial cells (HECs) changes from flat and adherent to spherical cells, which detach dorsal aorta. HECs attain a rounded shape in mitosis-independent manner before adhesion termination, suggesting an atypical cell-rounding mechanism. However, direct mechanisms underlying this change during EHT remain unclear. Here, we show that large vacuoles were transiently formed avian HECs, aquaporin 1 (AQP1) was localized vacuole plasma membranes. Overexpression AQP1 non-HECs induced ectopic expansion, rounding subsequent detachment endothelium into bloodstream, mimicking EHT. Loss redundant AQP functions by CRISPR/Cas9 gene editing impeded morphological Our findings provide first evidence indicate segregation regulated water influx vacuoles. These important insights further exploration cell/tissue morphogenesis through water-adoptive cellular responses.

Language: Английский

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Narrowed pore conformations of aquaglyceroporins AQP3 and GlpF

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 20, 2025

Aquaglyceroporins such as aquaporin−3 (AQP3) and its bacterial homologue GlpF facilitate water glycerol permeation across lipid bilayers. X-ray crystal structures of showed open pore conformations, AQP3 has also been predicted to adopt this conformation. Here we present cryo-electron microscopy rat in different narrowed conformations. In n-dodecyl-β-D-maltopyranoside detergent micelles, aromatic/arginine constriction filter residues containing Tyr212 form a 2.8-Å diameter pore, whereas 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) nanodiscs, inserts into the pore. Molecular dynamics simulation shows Tyr212-in conformation is stable largely suppresses permeability. reconstituted POPC liposomes exhibits permeability, suggesting that may be altered during permeation. Y212F Y212T mutant suggest aromatic residue drives pore-inserted The conserved AQP7 GlpF, but neither structure AQP3-like nanodiscs. Unexpectedly, covered by an intracellular loop, loop flexible not primarily related Our findings illuminate unique structural diversity aquaglyceroporins. aquaglyceroporin channels AQP3, AQP7, permeate glycerol, remains unclear. Here, authors AQP3-specific which selective tyrosine inserted

Language: Английский

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Acetazolamide as an aquaporin 1 inhibitor mitigates rheumatoid arthritis by reducing angiogenesis via the modulation of the FAK-PI3K/Akt signaling pathway

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 158, P. 114845 - 114845

Published: May 12, 2025

Language: Английский

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Aryl hydrocarbon receptor impairs circadian regulation in Alzheimer's disease: Potential impact on glymphatic system dysfunction

European Journal of Neuroscience, Journal Year: 2024, Volume and Issue: 60(2), P. 3901 - 3920

Published: June 25, 2024

Circadian clocks maintain diurnal rhythms of sleep-wake cycle 24 h that regulate not only the metabolism an organism but also many other periodical processes. There is substantial evidence circadian regulation impaired in Alzheimer's disease. properties known to be disturbed patients, such as integrity blood-brain barrier (BBB) well glymphatic flow controls waste clearance from brain. Interestingly, evolutionarily conserved transcription factor, is, aryl hydrocarbon receptor (AhR), impairs function core clock proteins and thus could disturb rhythmicity BBB. abundant activation AhR signalling inhibits expression major proteins, brain muscle arnt-like 1 (BMAL1), regulator (CLOCK) period (PER1) different experimental models. The robustly increased brains protein level enriched astrocytes It seems since it (i) BBB, which cooperatively interconnected with system brain, (ii) neuroinflammation dysbiosis gut microbiota generate potent activators AhR, are able impair flow. I will examine current indicating functions BBB involved development pathology.

Language: Английский

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The multifaceted role of aquaporins in physiological cell migration

AJP Cell Physiology, Journal Year: 2023, Volume and Issue: 325(1), P. C208 - C223

Published: May 29, 2023

Cell migration is an essential process that underlies many physiological processes, including the immune response, organogenesis in embryo, and angiogenesis, as well pathological processes such cancer metastasis. Cells have at their disposal a variety of migratory behaviors mechanisms seem to be specific cell type microenvironment. Research over past two decades has elucidated water channel protein family aquaporins (AQPs) regulator migration-related from physical phenomena biological signaling pathways. The roles AQPs play are both type- isoform-specific; thus, large swath information accumulated researchers seek identify responses across these distinct variables. There does not universal role migration; complex interplay between volume management, pathway activation, few identified circumstances, gene expression regulation, shown intricate, perhaps paradoxical, migration. objective this review provide organized integrated collection recent work by which regulate

Language: Английский

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Molecular mechanisms of saliva secretion and hyposecretion

European Journal Of Oral Sciences, Journal Year: 2024, Volume and Issue: 132(2)

Published: Jan. 8, 2024

Abstract The exocrine salivary gland secretes saliva, a fundamental body component to maintain oral homeostasis. Saliva is composed of water, ions, and proteins such as amylase, mucins, immunoglobulins that play essential roles in the digestion food, lubrication, prevention dental caries periodontitis. An increasing number people experience saliva hyposecretion due aging, medications, Sjögren's syndrome, radiation therapy for head neck cancer. However, current treatments are mostly limited temporary symptomatic relief. This review explores molecular mechanisms underlying secretion provide insight into putative therapeutic targets treatment. Proteins implicated pathways, including Ca 2+ ‐signaling proteins, aquaporins, soluble N‐ethylmaleimide‐sensitive factor attachment protein receptors, tight junctions, aberrantly expressed localized patients with hyposecretion, syndrome. Analysis studies on suggests crosstalk between fluid secretory pathways via /protein kinase C cAMP/protein A regulates secretion. Impaired two may contribute hyposecretion. Future research detailed regulatory information define novel generate strategies

Language: Английский

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Metabolomic Analysis of Stephania tetrandra–Astragalus membranaceus Herbal Pair-Improving Nephrotic Syndrome Identifies Activation of IL-13/STAT6 Signaling Pathway

Pharmaceuticals, Journal Year: 2023, Volume and Issue: 16(1), P. 88 - 88

Published: Jan. 8, 2023

The Stephania tetrandra−Astragalus membranaceus herbal pair (FH) is a classic widely used in the treatment of nephrotic syndrome (NS). effects tetrandra (FJ) and Astragalus (HQ) on NS have been reported, but mechanism their combination improvement are still unclear. model was established by injecting adriamycin into tail vein. FH intervention reduced levels serum triglyceride, total cholesterol, interleukin-6 (IL-6), blood urea nitrogen (BUN), urinary protein, gene expression aquaporin 2 (AQP2) arginine vasopressin (AVP) rats. In addition, improved kidney injury rats inhibiting interleukin 13 (IL-13), phospho-signal transducers, activators transcription 6 (p-STAT6), Bax, cleaved-caspase3, while promoting Bcl-2. By comprehensive comparison multiple indexes, lipid metabolism, glomerular filtration rate, inflammation were superior to that FJ HQ. Metabonomic studies showed that, compared with HQ, significantly regulated tricarboxylic acid (TCA) cycle, cysteine methionine alanine, aspartic glutamic metabolism. Pearson correlation analysis succinic L-aspartic negatively correlated cystatin C (Cys C) BUN (p < 0.05). summary, could reduce renal improve through IL-13/STAT6 signal pathway, regulating endogenous metabolic pathways, such as TCA AQP2 AVP genes. This study provides strategy reveal NS, also reasonable way clarify compatibility traditional Chinese medicine.

Language: Английский

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Aquaporin-7-Mediated Glycerol Permeability Is Linked to Human Sperm Motility in Asthenozoospermia and during Sperm Capacitation

Cells, Journal Year: 2023, Volume and Issue: 12(15), P. 2003 - 2003

Published: Aug. 5, 2023

Osmoregulation plays a vital role in sperm function, encompassing spermatogenesis, maturation, and fertilization. Aquaglyceroporins, subclass of aquaporins (AQPs), facilitate the transport water glycerol across membrane, with serving as an important substrate for bioenergetics. This study aimed to elucidate significance AQP-mediated permeability motility. The presence localization AQP3 AQP7 human were assessed using immunofluorescence. Subsequently, spermatozoa obtained from normozoospermic individuals (n = 30) was measured, stopped-flow light scattering, after incubation specific aquaporin inhibitors targeting (DFP00173), (Z433927330), or general aquaglyceroporin (phloretin). Sperm asthenozoospermic men utilized evaluate AQP7-mediated permeability, compare it that men. Furthermore, hypermotile capacitated cells examined, determine expression membrane permeability. predominantly observed tail region, while present head, midpiece, sperm. Our findings indicate key inhibition resulted 55% decrease diffusion membrane. Importantly, this impairment evident individuals, suggesting dysregulation transport, despite similar levels. Conversely, increased sperm, compared non-capacitated Hence, may serve valuable indicator motility, be crucial function.

Language: Английский

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