Current Medicinal Chemistry, Journal Year: 2023, Volume and Issue: 31(31), P. 4987 - 5003
Published: July 27, 2023
Cardiovascular diseases remain the leading cause of death worldwide; therefore, there is increasing attention to developing physiological-related in vitro cardiovascular tissue models suitable for personalized healthcare and preclinical test. Recently, more complex powerful
Language: Английский
Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 511 - 536
Published: June 27, 2023
Bioinks are formulations of biomaterials and living cells, sometimes with growth factors or other biomolecules, while extrusion bioprinting is an emerging technique to apply deposit these bioinks biomaterial solutions create three-dimensional (3D) constructs architectures mechanical/biological properties that mimic those native human tissue organs. Printed have found wide applications in engineering for repairing treating tissue/organ injuries, as well vitro modelling testing validating newly developed therapeutics vaccines prior their use humans. Successful printing subsequent rely on the formulated bioinks, including rheological, mechanical, biological properties, process. This article critically reviews latest developments bioprinting, focusing bioink synthesis characterization, influence Key issues challenges also discussed along recommendations future research.
Language: Английский
Citations
98
Nanomaterials, Journal Year: 2024, Volume and Issue: 14(6), P. 531 - 531
Published: March 16, 2024
Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic adds complexity to traditional cell cultivation methods. most commonly used technique involves cultivating cells on flat surface in two-dimensional format due its simplicity. A three-dimensional (3D) provide microenvironment for surrounding cells. There two main techniques obtaining 3D structures based the presence scaffolding. Scaffold-free consist spheroid technologies. Meanwhile, scaffold contain organoids all constructs that various types scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels one extensively studied forms potential culture up 4D bioprinted biomaterials. bioprinting is important create scaffolds. versatility this allows many different inks, mainly hydrogels, as well inorganic substances. Increasing amounts data evidence vast usage engineering personalized medicine, with area application being regeneration skin musculoskeletal systems. Recent papers also indicate increasing vivo tests products which further strengthen importance branch emphasize need extensive research safe humansbiomimetic tissues organs. In review article, we recent advancements field preceded by an overview technologies led development complex type culture.
Language: Английский
Citations
22
Molecular and Cellular Biochemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 21, 2025
Abstract Endothelial cells (ECs) are arranged side-by-side to create a semi-permeable monolayer, forming the inner lining of every blood vessel (micro and macrocirculation). Serving as first barrier for circulating molecules cells, ECs represent main regulators vascular homeostasis being able respond environmental changes, either physical or chemical signals, by producing several factors that regulate tone cellular adhesion. Healthy endothelium has anticoagulant properties prevent adhesion leukocytes platelets walls, contributing resistance thrombus formation, regulating inflammation, smooth muscle cell proliferation. Many risk cardiovascular diseases (CVDs) promote endothelial expression chemokines, cytokines, molecules. The resultant activation can lead dysfunction (ECD). In vitro models ECD allow study molecular mechanisms disease provide research platform screening potential therapeutic agents. Even though alternative available, such animal ex vivo models, in offer higher experimental flexibility reproducibility, making them valuable tool understanding pathophysiological diseases, CVDs. Therefore, this review aims synthesize currently available regarding ECD, emphasizing This work will focus on 2D culture (endothelial lines primary ECs), 3D systems (scaffold-free scaffold-based), (such organ-on-a-chip). We dissect role external stimuli—chemical mechanical—in triggering ECD.
Language: Английский
Citations
1
Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11
Published: May 25, 2023
Three-dimensional (3D) bioprinting is an advanced tissue engineering technique that has received a lot of interest in the past years. We aimed to highlight characteristics articles on 3D bioprinting, especially terms research hotspots and focus. Publications related from 2007 2022 were acquired Web Science Core Collection database. have used VOSviewer, CiteSpace, R-bibliometrix perform various analyses 3,327 published articles. The number annual publications increasing globally, trend expected continue. United States China most productive countries with closest cooperation development investment funds this field. Harvard Medical School Tsinghua University are top-ranked institutions China, respectively. Dr. Anthony Atala Ali Khademhosseini, researchers may provide opportunities for interested researchers. Tissue Engineering Part A contributed largest publication number, while Frontiers Bioengineering Biotechnology was attractive journal potential. As keywords Bio-ink, Hydrogels (especially GelMA Gelatin), Scaffold decellularized extracellular matrix), extrusion-based engineering, vitro models (organoids particularly) analyzed current study. Specifically, topics “new bio-ink investigation,” “modification cell viability vascularization,” “application organoids model” “research personalized regenerative medicine” predicted be future research.
Language: Английский
Citations
22
Advanced Materials, Journal Year: 2023, Volume and Issue: 35(35)
Published: April 27, 2023
Abstract Cancer kills millions of individuals every year all over the world (Global Observatory). The physiological and biomechanical processes underlying tumor are still poorly understood, hindering researchers from creating new, effective therapies. Inconsistent results preclinical research, in vivo testing, clinical trials decrease drug approval rates. 3D tumor‐on‐a‐chip (ToC) models integrate biomaterials, tissue engineering, fabrication microarchitectures, sensory actuation systems a single device, enabling reliable studies fundamental oncology pharmacology. This review includes critical discussion about their ability to reproduce microenvironment (TME), advantages drawbacks existing architectures, major components techniques. focus is on current materials micro/nanofabrication techniques used manufacture reproducible microfluidic ToC for large‐scale trial applications.
Language: Английский
Citations
20
Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(5)
Published: May 1, 2023
Liver disease is one of the serious threats to human life and health. Three-dimensional (3D) liver models, which simulate structure function natural tissue in vitro, have become a common demand medical, scientific pharmaceutical fields nowadays. However, complex cellular composition multi-scale spatial arrangement make it extremely challenging construct models vitro. According HepaRG preference printing strategy, formulation bioink system with opposite charge optimized. The sodium alginate-based 1 dipeptide-based 2 are used ensure structural integrity provide flexible designability, respectively. HepaRG/HUVECs/LX-2-laden organoids biomimetic lobule fabricated by multicellular 3D droplet-based bioprinting mimic cell heterogeneity, extracellular matrix (ECM) features. can maintain distribution within printed lobule-like after 7 days culture. Compared 2D monolayer culture, constructed show high viability, ALB secretion urea synthesis levels. This study provides layer-by-layer strategy for vitro construction structure, giving meaningful insights new drugs, modelling, regeneration.
Language: Английский
Citations
18
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2512 - 2512
Published: Feb. 21, 2024
Bone differentiation is crucial for skeletal development and maintenance. Its dysfunction can cause various pathological conditions such as rickets, osteoporosis, osteogenesis imperfecta, or Paget's disease. Although traditional two-dimensional cell culture systems have contributed significantly to our understanding of bone biology, they fail replicate the intricate biotic environment tissue. Three-dimensional (3D) spheroid cultures gained widespread popularity addressing defects. This review highlights advantages employing 3D investigate differentiation. It their capacity mimic complex in vivo cellular interactions pivotal homeostasis. The exploration models research offers enhanced physiological relevance, improved predictive capabilities, reduced reliance on animal models, which advancement safer more effective strategies drug development. Studies highlighted transformative potential expanding biology developing targeted therapeutic interventions bone-related disorders. explores how demonstrated promise unraveling mechanisms governing homeostasis responses pharmacological agents.
Language: Английский
Citations
8
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130604 - 130604
Published: March 5, 2024
Language: Английский
Citations
8
Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)
Published: Dec. 16, 2023
In vitro models are necessary to study the pathophysiology of disease and development effective, tailored treatment methods owing complexity heterogeneity breast cancer large population affected by it. The cellular connections tumor microenvironments observed in vivo often not recapitulated conventional two-dimensional (2D) cell cultures. Therefore, developing 3D that mimic complex architecture physiological circumstances tumors is crucial for advancing our understanding illness. A scaffold-free model mimics allowing cells self-assemble/pattern into structures, contrast with other rely on artificial scaffolds. It possible this model, whether applied using patient-derived primary (fibroblasts, endothelial cells, cells), can accurately replicate heterogeneity. complicated interactions between different types modelled integrating critical components microenvironment, such as extracellular matrix, vascular growth factors. Tissue interactions, immune infiltration, effects milieu drug resistance be studied model. mimicking a useful tool studying molecular basis disease, identifying new therapeutic targets, evaluating modalities. provides more physiologically appropriate high-throughput platform screening compound library 96-384 well format. We critically discussed rapid personalized strategies accelerated platforms close gap traditional 2D culture investigations.
Language: Английский
Citations
15
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: May 1, 2024
Abstract Stem cells (SCs) have been used therapeutically for decades, yet their applications are limited by factors such as the risk of immune rejection and potential tumorigenicity. Extracellular vesicles (EVs), a key paracrine component stem cell potency, overcome drawbacks cell-free therapeutic agent play an important role in treating various diseases. However, EVs derived from two-dimensional (2D) planar culture SCs low yield face challenges large-scale production, which hinders clinical translation EVs. Three-dimensional (3D) culture, given its ability to more realistically simulate vivo environment, can not only expand large quantities, but also improve activity EVs, changing content improving effects. In this review, we briefly describe advantages EV-related applications, provide overview 3D finally focus on specific future perspectives different SCs. Graphical
Language: Английский
Citations
6