International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(19), P. 14722 - 14722
Published: Sept. 29, 2023
Cellular
asymmetry
is
an
important
element
of
efficiency
in
the
compartmentalization
intracellular
chemical
reactions
that
ensure
efficient
tissue
function.
Improving
current
3D
printing
methods
by
using
cellular
essential
producing
complex
tissues
and
organs
such
as
liver.
The
use
cell
spots
containing
at
least
two
cells
basement
membrane-like
bio
support
materials
allows
to
be
tethered
points
on
membrane
with
another
order
maintain
asymmetry.
Our
model
a
new
type
bioprinter
uses
oriented
multicellular
complexes
This
novel
approach
necessary
replace
sequential
slow
processes
organogenesis
rapid
growth
organ
printing.
extracellular
matrix
process
bioprinting
one
preserve
thus
biological
metabolic
efficiency.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: March 25, 2024
Gelatin
Methacryloyl
(GelMA)
is
one
of
the
most
used
biomaterials
for
a
wide
range
applications,
such
as
drug
delivery,
disease
modeling
and
tissue
regeneration.
GelMA
obtained
from
gelatin,
which
can
be
derived
different
sources
(e.g.,
bovine
skin,
porcine
skin),
through
substitution
reactive
amine
hydroxyl
groups
with
methacrylic
anhydride
(MAA).
The
degree
functionalization
(DoF)
tuned
by
varying
MAA
amount
used;
thus,
protocols,
reaction
efficiency,
have
been
developed,
using
various
alkaline
buffers
phosphate-buffered
saline,
DPBS,
or
carbonate-bicarbonate
solution).
Obviously,
DoF
modulation
has
an
impact
on
final
properties,
so
deep
investigation
features
hydrogel
must
carried
on.
purpose
this
study
to
investigate
how
gelatin
synthesis
methods
affect
literature
lacks
direct
systematic
comparisons
between
these
parameters,
especially
methods.
aim
facilitate
choice
source
method
according
needs
desired
application.
Hence,
chemical
physical
properties
formulations
were
assessed,
determining
DoFs,
mechanical
viscoelastic
rheological
analysis,
water
absorption
swelling
capacity
enzymatic
degradation
rates.
Biological
tests
lung
adenocarcinoma
cells
(A549)
performed.
Moreover,
since
3D
bioprinting
rapidly
evolving
technology
thanks
possibility
precise
deposition
cell-laden
(bioinks)
mimic
structures
several
tissues,
potential
bioinks
tested
multi-material
approach,
revealing
its
printability
versatility
in
applications.
Abstract
With
the
aging
and
obesity
era,
increasing
incidence
of
diabetes
diabetic
complications,
especially
non‐healing
wounds,
imposes
a
serious
economic
burden
on
both
patients
society.
The
complex
microenvironments,
including
hyperglycemia,
bacterial
infection,
ischemia,
nerve
damage,
lead
to
prolonged
inflammation
proliferation
phase
wounds.
Mesenchymal
stem
cell‐derived
small
extracellular
vesicles
(MSC‐sEVs),
which
contain
rich
variety
therapeutic
molecules,
have
been
chased
for
decades
because
their
potential
roles
in
cellular
communication,
tissue
regeneration,
drug
delivery.
As
powerful
tools
controlled‐sustained
release
sEVs,
biocompatible
hydrogels
applied
wide
range
biomedical
applications.
Herein,
we
first
summarize
pathological
features
such
as
angiopathy,
neuropathy,
immune
cell
dysfunction.
Then,
discuss
biological
properties,
performance,
stability
pure
MSC‐sEVs.
After
that,
components,
application
patterns,
responsiveness
hydrogels.
Next,
loading
avenues
MSC‐sEVs
into
hydrogel,
behaviors
sEVs
from
hydrogels,
influence
crosslinking
method
hydrogel‐sEV
composites.
Finally,
provide
an
overview
current
applications
loaded
with
novel
cell‐free
engineering
system
managing
wounds
propose
critical
unsolved
issues.
This
review
is
expected
meaningful
guidance
developing
wound
management.
Journal of Pain Research,
Journal Year:
2023,
Volume and Issue:
Volume 16, P. 3441 - 3462
Published: Oct. 1, 2023
Background:
Biomechanical
research
on
the
lumbar
intervertebral
disc
(IVD)
provides
valuable
information
for
diagnosis,
treatment,
and
prevention
of
related
diseases,
has
received
increasing
attention.
Using
bibliometric
methods
visualization
techniques,
this
study
investigates
first
time
status
development
trends
in
field,
with
aim
providing
guidance
support
subsequent
research.
Methods:
The
Science
Citation
Index
Expanded
(SCI-Expanded)
within
Web
Core
Collection
(WoSCC)
database
was
used
as
data
source
to
select
literature
published
from
2003
2022
biomechanical
IVD.
VOSviewer
1.6.19
CiteSpace
6.2.R2
software,
well
online
analysis
platform
metrology,
were
utilized
generate
scientific
knowledge
maps
visual
display
analysis.
Results:
United
States
is
most
productive
country
Ulm
University
making
largest
contribution.
Wilke
HJ
both
prolific
author
one
highly
cited
authors,
while
Adams
MA
author.
Spine,
J
Biomech,
Eur
Spine
J,
,
Clin
Biomech
are
not
only
journals
highest
number
publications,
but
also
journals.
main
topics
field
include
constructing
validating
three-dimensional
(3D)
finite
element
model
(FEM)
spine,
measuring
intradiscal
pressure,
exploring
effects
risk
factors
degeneration,
studying
mechanical
responses
different
torque
load
combinations,
classifying
degeneration
based
magnetic
resonance
images
(MRI),
which
hot
themes
recent
years.
Conclusion:
This
systematically
reviews
system
biomechanics
IVD,
references
further
Keywords:
disc,
biomechanics,
bibliometric,
analysis,
VOSviewer,
Health Science Reports,
Journal Year:
2024,
Volume and Issue:
7(6)
Published: June 1, 2024
Abstract
Background
and
Aims
The
advent
of
3D
printing
has
revolutionized
plastic
surgery
prosthetic
devices,
providing
personalized
solutions
for
patients
with
traumatic
injuries,
deformities,
appearance‐related
conditions.
This
review
offers
a
comprehensive
overview
printing's
applications,
advantages,
limitations,
future
prospects
in
these
fields.
Methods
A
literature
search
was
conducted
PubMed,
Google
Scholar,
Scopus
studies
on
surgery.
Results
significantly
contributed
to
medical
interventions,
benefits
like
enhanced
design
flexibility,
reduced
production
time,
improved
patient
outcomes.
Using
computer‐aided
(CAD)
software,
precise
models
tailored
patient's
anatomy
can
be
created,
ensuring
better
fit,
functionality,
comfort.
allows
intricate
geometries,
leading
aesthetic
outcomes
patient‐specific
limbs
orthoses.
historical
development
printing,
key
milestones,
breakthroughs
are
highlighted.
Recent
progress
bioprinting
tissue
engineering
shows
promising
applications
regenerative
medicine
transplantation.
integration
AI
automation
enhances
surgical
planning
Emerging
trends
treatment
precision
potential
game‐changers.
However,
challenges
technical
considerations,
economic
implications,
ethical
issues
exist.
Addressing
advancing
research
materials,
processes,
long‐term
crucial
widespread
adoption.
Conclusion
underscores
the
increasing
adoption
healthcare
its
impact
devices.
It
emphasizes
importance
evaluating
current
state
addressing
knowledge
gaps
through
foster
further
advancements
Bioengineering,
Journal Year:
2024,
Volume and Issue:
11(9), P. 862 - 862
Published: Aug. 23, 2024
This
paper
reports
an
experimental
study
on
the
compatibility
of
human
bronchial
epithelial
(HBE)
cells
in
a
collagen-alginate
bioink.
The
was
assessed
using
culture
well
method
with
three
bioink
compositions
prepared
from
10%
alginate
solution
and
neutralized
TeloCol-10
mg/mL
collagen
stock
solution.
Cell
viability,
quantified
by
(live
cell
count-dead
count)/live
count
within
HBE
cell-laden
hydrogel,
evaluated
live/dead
assay
Day
0
to
6.
Experimental
results
demonstrated
that
4:1
composition
exhibited
highest
viability
6
(85%),
outperforming
1:4
composition,
which
showed
75%
45%,
respectively.
Additionally,
live
for
0,
trend
persisted
through
Days
1
6,
underscoring
its
superior
performance
maintaining
promoting
proliferation.
These
findings
show
higher
compared
other
two
compositions.
3D Printing in Medicine,
Journal Year:
2025,
Volume and Issue:
11(1)
Published: Feb. 17, 2025
Bioprinting
is
a
tissue
engineering
technique
that
rapidly
evolving
to
include
complex
clinical
applications.
However,
there
limited
evidence
describing
how
far
bioprinting
has
progressed
past
the
pre-clinical
stage.
Thus,
we
conducted
scoping
review
assess
landscape
of
studies,
including
interventional
and
observational
trials,
involving
by
charting
trends
in
general
characteristics,
application,
trial
design.
The
term
"bioprint"
its
variants
were
searched
five
databases
(ICTRP,
ScanMedicine,
CENTRAL,
NIHCC,
HCCTD)
two
registries
(ClinicalTrials.gov,
PHRR)
on
22
February
2024.
This
was
followed
duplicate
removal
dual
independent
finalize
inclusion
list.
We
included
trials
published
or
translated
English
mentioning
their
design,
while
excluded
those
did
not
adhere
our
definition
bioprinting.
Finally,
data
charted
synthesized
narratively.
Of
36
total
search
records,
11
met
criteria.
Registration
dates
ranged
from
2016
2023,
with
China
conducting
most
globally.
Four
had
results,
remaining
still
progress.
aimed
implant
bioprinted
tissues
made
autologous
cells,
blood
vessels,
trachea,
external
ear,
wound
dressings.
other
seven
studies
aiming
bioprint
cell-laden
vitro
models
study
conditions
such
as
cancer.
early
stages
research,
focus
producing
patient-specific
for
cancer
precision
medicine
regenerative
purposes.
More
standardized
reporting
bioprinting-related
information
needed
improve
research
transparency
replicability.
As
body
grows,
may
be
used
framework
monitor
translation
over
years.
Journal of Multidisciplinary Healthcare,
Journal Year:
2025,
Volume and Issue:
Volume 18, P. 1249 - 1268
Published: Feb. 28, 2025
Pelvic
organ
prolapse
(POP)
has
become
a
significant
public
health
issue,
with
its
prevalence
increasing
proportionally
age.
Despite
the
considerable
number
of
biomechanical
studies
reported
on
POP,
there
remains
lack
systematic
approach
to
summarize
and
synthesize
all
existing
research.
The
Web
Science
Core
Collection
(WoSCC)
database
was
used
as
data
source
select
literature
published
from
2003
2023
related
research
POP.
We
employed
various
visualization
software
generate
scientific
knowledge
maps,
facilitating
analysis
visual
representation.
This
study
included
292
publications,
comprising
252
articles
(86.3%)
40
review
(13.7%).
United
States
emerged
leading
nation
in
terms
productivity,
University
Porto
making
contributions.
Robust
partnerships
are
maintained
by
countries
institutions
involved.
Moali
PA
stands
out
most
prolific
author,
while
Deprest
J
exhibits
exemplary
levels
collaboration.
Notably,
journal
Int
Urogynecol
highest
publication
rate
citation
frequency,
contribution
demonstrating
academic
influence
field.
Keyword
cluster
reveal
that
key
areas
include
validating
finite
element
(FE)
models
pelvic
floor
structures,
studying
interactions
among
support
systems,
evaluating
impact
vaginal
delivery,
assessing
effects
mesh
or
3D-printed
materials
POP
repair,
remodeling
connective
tissue
patients,
performance
evaluations
tissues.
Future
will
likely
focus
development
personalized
regenerative
treatment
strategies.
Moreover,
advancements
machine
learning,
medicine
approaches,
multimodal
large-scale
FE
modeling
offer
promising
insights
for
development.
presents
comprehensive
system
directions
biomechanics
providing
valuable
guidance
future
endeavors.
Pharmaceutics,
Journal Year:
2025,
Volume and Issue:
17(4), P. 456 - 456
Published: April 1, 2025
Advances
in
drug
delivery
systems
adapted
with
regenerative
medicine
have
transformed
healthcare
by
introducing
innovative
strategies
to
treat
(and
repair
many
instances)
disease-impacted
regions
of
the
human
body.
This
review
provides
a
comprehensive
analysis
latest
developments
and
challenges
integrating
technologies
medicine.
Recent
advances
technologies,
including
design
biomaterials,
localized
techniques,
controlled
release
guided
mathematical
models,
are
explored
illustrate
their
role
enhancing
therapeutic
precision
efficacy.
Additionally,
approaches
analyzed,
focus
on
extracellular
matrix
components,
stem
cell-based
therapies,
emerging
for
organ
regeneration
both
soft
hard
tissue
vitro
model
engineering.
In
particular,
also
discusses
applications
cellular
cells,
immune
endothelial
specialized
cells
such
as
chondrocytes
osteoblasts,
highlights
advancements
cell
methods
cell–cell
interaction
modulation.
addition,
future
directions
pivotal
trends
emphasizing
importance
interdisciplinary
collaboration
cutting-edge
innovations
provided
address
successful
outcomes
