Temporomandibular Joint Degeneration Arises Spontaneously in STR/Ort Mice and is Prevented by Targeted Aggrecanase Inhibition DOI Creative Commons
Kazuhiro Ooi, Kazuhiro Yamamoto, Yutaka Kobayashi

et al.

Osteoarthritis and Cartilage Open, Journal Year: 2025, Volume and Issue: 7(2), P. 100599 - 100599

Published: March 12, 2025

Temporomandibular joint osteoarthritis (TMJ-OA) is painful and causes masticatory dysfunction, but current treatment limited to symptom relief due an incomplete appreciation of aetiology. Herein, we develop morphological histological methods for quantitative evaluation TMJ-OA severity examine whether STR/Ort mice, which are genetically predisposed spontaneous knee OA, exhibit protection against upon genetic gain-of-function modification aggrecanase-selective mutant tissue inhibitor metalloproteinase (TIMP)-3. We established changes in mandibular condylar head adapted from human criteria, developed verified the utility damage scoring OARSI system. Mutant TIMP3 containing extra alanine at N-Terminus ([-1A] TIMP-3 was overexpressed CBA mice. Morphological condyle TMJ cartilage degradation were evaluated quantified using micro-CT histology mice aged 10, 20 40 weeks. Whilst no evidence observed 10 weeks, bone erosion osteophyte formation appeared by with remarkable deformity resorption weeks STR/Ort, not parental strain. less severe week-old [-1A]TIMP-3 overexpressing compared wild-type Using our new mouse system have found that OA affects joints other than Genetic (TIMP)-3 also affords vivo chondroprotection this TMJ-OA.

Language: Английский

Challenges in Optimizing Nanoplatforms Used for Local and Systemic Delivery in the Oral Cavity DOI Creative Commons
Dorin Ioan Cocoș, Olimpia Dumitriu Buzia, Alin Laurenţiu Tatu

et al.

Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(5), P. 626 - 626

Published: May 7, 2024

In this study, we focused on innovative approaches to improve drug administration in oral pathology, especially by transmucosal and transdermal pathways. These improvements refer the type of microneedles used (proposing needles saw), use certain enhancers such as essential oils (which, besides amplifier action, also have intrinsic actions health), associations active substances with synergistic well copolymeric membranes, cemented directly tooth. We propose a review principles release at level mucosa main systems pathology. Controlled failure applicable pathology include following: fast dissolving films, mucoadhesive tablets, hydrogels, intraoral composite wafers, smart drugs. The novelty elements brought paper possibilities optimizing localized delivery system osteoarthritis temporomandibular joint, neuropathic pain, cancer, periodontitis, pericoronitis, maintaining health. would like mention possibility incorporating natural products into controlled paying special attention oils.

Language: Английский

Citations

20
Fostamatinib alleviates temporomandibular joint osteoarthritis by maintaining cartilage homeostasis through MAPK/NF-κB and AKT/mTOR pathways DOI
Yuxin Zhang, Dahe Zhang, Qingyu Xu

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 113996 - 113996

Published: Jan. 5, 2025

Language: Английский

Citations

1
Decoding Chronic Jaw Pain: Key Nature of Temporomandibular Disorders in Slovak Patients DOI Open Access
Peter Kizek, Veronika Pačutová, Vladimíra Schwartzová

et al.

Bratislavské lekárske listy/Bratislava medical journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract Background Temporomandibular disorder (TMD) affects the jaw joint and muscles, causing pain, movement issues symptoms like clicking sounds. More common in women, TMD is linked to factors stress, bruxism posture. It often coexists with headaches neck causes being a mix of physical psychological factors. Therefore, we decided examine Slovak patients better understand their specific experiences. Methods Patients were recruited from Department Stomatology Maxillofacial Surgery at University Hospital Slovakia 2017 2024, resulting sample 380 patients. Inclusion criteria required adults aged 18–90 symptoms, such as pain difficulties, diagnosed by maxillofacial surgeon. completed paper-based questionnaires on background information; characteristics symptoms; medical history, prior treatment. Results We confirm that predominantly female (81.1%) tends be chronic condition, 58.6% having experienced it for over 6 months. A significant proportion reported moderate severe (42–26.4%) nearly half (43.7%) constant pain. Movement-triggered was (67.8%), most sound phenomena (96.9%) difficulty opening mouth (65.6%). Stress played role, 43.7% experiencing higher emotional burden 32.4% reporting stress-related worsening symptoms. Additionally, 46.3% worked sedentary environments, potentially contributing condition. Conclusion Most experience multidimensional interplay physical, social

Language: Английский

Citations

1
S-propargyl-cysteine attenuates temporomandibular joint osteoarthritis by regulating macrophage polarization via Inhibition of JAK/STAT signaling DOI Creative Commons

Wenyi Cai,

Antong Wu,

Zhongxiao Lin

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: April 7, 2025

Abstract Background Temporomandibular joint osteoarthritis (TMJ-OA) is a disease characterized by cartilage degradation and synovial inflammation, with limited effective treatment currently. Synovial macrophage polarization pivotal in TMJ-OA progression, making it promising therapeutic aspect. This study investigated the effects of S-propargyl-cysteine (SPRC), an endogenous H2S donor, on its potential alleviating TMJ-OA. Methods A MIA-induced rat model LPS-stimulated RAW264.7 macrophages were employed to evaluate SPRC vivo vitro. TMJ bone analyzed via micro-CT histological methods, while markers expression assessed RT-qPCR, western blot, immunofluorescence. RNA sequencing was performed macrophages, JAK2/STAT3 signaling pathway validated using JAK2-specific inhibitor AG490. The direct primary condylar chondrocytes examined evaluating ECM synthesis degradation. Co-culture experiments further macrophage-chondrocyte interactions. Results significantly alleviated damage model, as demonstrated improved volume structure. reduced pro-inflammatory M1 infiltration enhanced anti-inflammatory M2 polarization. effectively inhibited JAK2/STAT3, leading reduction inflammatory markers, including TNF-α, IL-6, iNOS. revealed that SPRC-treated macrophage-conditioned medium chondrocyte metabolic activity restored integrity. Conclusions SPRC-modulated alleviates JAK/STAT downregulation, thereby reducing inflammation These findings position candidate for provide insights into novel strategies targeting synovium-cartilage crosstalk. Graphical abstract

Language: Английский

Citations

1
Equine Models of Temporomandibular Joint Osteoarthritis: A Review of Feasibility, Biomarkers, and Molecular Signaling DOI Creative Commons
Tomasz Jasiński, Ḃ. Turek, Michał Kaczorowski

et al.

Biomedicines, Journal Year: 2024, Volume and Issue: 12(3), P. 542 - 542

Published: Feb. 28, 2024

Osteoarthritis (OA) of the temporomandibular joint (TMJ) occurs spontaneously in humans and various animal species, including horses. In humans, obtaining tissue samples is challenging clinical symptoms appear late disease progression. Therefore, genetically modified, induced, naturally occurring models play a crucial role understanding pathogenesis evaluating potential therapeutic interventions for TMJ OA. Among models, equine OA model characterized by slow, age-related progression, wide range examinations, imaging modalities that can be performed on horses, as well easy synovial fluid collection. The morphological functional similarities structures both species make an excellent opportunity to track progression response treatment. However, much work remains carried out determine utility human biomarkers main biomarkers, IL-1, IL-6, TGF-β, TNF-α, PGE2 have been recently investigated model. majority cartilage degradation, chondrocyte hypertrophy, angiogenesis, overload—as any signaling pathways—have not studied so far. it would advisable focus further research specimens, considering mediators signaling.

Language: Английский

Citations

7
Endothelial Stat3 activation promotes osteoarthritis development DOI Creative Commons
Jiadong Li, Wen-Cai Zhang, Xinru Liu

et al.

Cell Proliferation, Journal Year: 2023, Volume and Issue: 56(12)

Published: June 13, 2023

Abstract The mechanism of the balance between subchondral angiogenesis and articular damage within osteoarthritis (OA) progression remains a mystery. However, lack specific drugs leads to limited clinical treatment options for OA, frequently failing prevent eventual joint destruction in patients. Increasing evidence suggests that bone precedes cartilage injury, while proliferating endothelial cells (ECs) induce abnormal formation. Signal transducer activator transcription 3 (Stat3) is triggered by multiple cytokines OA microenvironment. Here, we observed elevated Stat3 activation H‐type vessels. Endothelial will lead stronger cell proliferation, migration simulating ECs OA. In contrast, either inhibition or knockdown expression could relieve such alterations. More interestingly, blocking alleviated angiogenesis‐mediated osteogenic differentiation chondrocyte lesions. inhibitor reversed surgically induced vessel hyperplasia vivo, significantly downregulating volume number. Due reduced angiogenesis, deterioration loss were alleviated. Overall, our data suggest an essential trigger development. Therefore, targeted blockade novel promising therapeutic regimen

Language: Английский

Citations

14
Platelet-rich plasma therapy for temporomandibular joint osteoarthritis: A randomized controlled trial DOI
Shasha Liu, Lili Xu, Likun Liu

et al.

Journal of Cranio-Maxillofacial Surgery, Journal Year: 2023, Volume and Issue: 51(11), P. 668 - 674

Published: Oct. 7, 2023

Language: Английский

Citations

13
Botulinum toxin A attenuates osteoarthritis development via inhibiting chondrocyte ferroptosis through SLC7Al1/GPX4 axis DOI
Lian Zeng, Yanping Liu,

Qingsong Wang

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(5), P. 167215 - 167215

Published: May 6, 2024

Language: Английский

Citations

5
Clinical effectiveness of polynucleotides TMJ Injections compared to physiotherapy. A 3 months randomized clinical trial DOI Creative Commons
Niccolò Cenzato,

Roberta Crispino,

A. Russillo

et al.

British Journal of Oral and Maxillofacial Surgery, Journal Year: 2024, Volume and Issue: 62(9), P. 807 - 812

Published: July 20, 2024

IntroductionJoint visco-supplementation is an effective therapeutic approach against signs and symptoms of osteoarthrosic/arthritic degeneration mandibular condyle. Supplements choice are usually delivered inside TMJs through intra-articular infiltrations. The present study aims to assess the clinical effectiveness peri-capsular injections polinucleotides (PN) hyaluronic acid (HA).Materials methods60 patients suffering from TMJ osteoarthrosis were recruited divided in 2 groups (test group: n=30, injections; control self-delivered physiotherapeutic exercises). For each patient, maximum opening, right left lateral excursion VAS recorded at 4 different timepoints by a blinded investigator. Paired Student's t-test was used compare consecutive measurements parameters evaluated within groups. Unpaired student's for between-group comparisons.ResultsAll investigated displayed progressive improvement both groups, which more accentuated test group. decrease score differed significantly between (p<0.0001); opening showed continuous increase baseline (37.70±8.33 mm) T3 (39.68±7.64 mm), reflecting improvement, but it not statistically significant (P=0.089 respect T0).ConclusionPN HA represent minimally invasive treatment option reducing pain improving kinematic.

Language: Английский

Citations

5
Therapeutic Effects of PEG-Modified Polyamide Amine Dendrimer for Cell Free DNA Adsorption in Temporomandibular Joint Osteoarthritis DOI

Yuzhuo Hei,

Jingyi Du,

Zhizhao Deng

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(30), P. 39153 - 39164

Published: July 17, 2024

Temporomandibular joint osteoarthritis (TMJ OA) is characterized by the degeneration of cartilage and subchondral bone. In this study, we observed a significant increase in cell-free DNA (cfDNA) levels during progression TMJ OA. Bioinformatics analysis identified TLR9 as pivotal molecule OA pathogenesis. The polyamidoamine (PAMAM) dendrimer well-structured, highly branched, reactive nature, exhibits robust binding clearance capabilities for cfDNA. However, abundant amino groups on surface PAMAM lead to its inherent toxicity. To mitigate this, PEG-5000 was conjugated dendrimers, enhancing safety. Our results indicate that PEG-PAMAM effectively inhibits upregulation protein OA, significantly suppressing activation p-IκBα/p-NF-κB signaling pathway subsequently decreasing chondrocyte inflammation apoptosis, evidenced both vivo vitro experiments. We conclude safe effective material applications, offering promising therapeutic strategy targeting cfDNA clearance.

Language: Английский

Citations

4