Single-Cell RNA-Seq Analysis Reveals Four Stages of Replicative Senescence in Human Umbilical Vein Endothelial Cells DOI Creative Commons

Minseo Ahn,

Junil Kim, Jae Ho Seo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 15, 2023

Abstract Cellular senescence is a phenomenon marked by an irreversible growth arrest with altered physiological properties. Many studies have focused on the characteristics of cells that already entered senescent state. However, to elucidate mechanisms cellular aging, it essential investigate gradual transition proliferative into cells. We assumed complex and multi-step process, each stage exhibits unique traits. To test this hypothesis, we utilized publicly available single-cell RNA-Seq (scRNA-Seq) data from human umbilical vein endothelial (HUVECs) undergoing replicative senescence. employed Seurat Monocle 3 capture proliferating states in HUVECs. Four clusters were identified, cluster displayed distinct expression patterns markers senescence-associated secretory phenotypes (SASPs). also SCENIC identify core transcription factors (TFs) during While majority TFs exhibited linear trend, HMGB1, FOSL1, SMC3, RAD21, SOX4, XBP1 showed fluctuating Furthermore, these slight differences ionizing radiation (IR) model Overall, our study unveils phase identifies trends may play pivotal roles process. These findings highlight intricate nature provide new insights process aging.

Language: Английский

The Expression Level of SOX Family Transcription Factors’ mRNA as a Diagnostic Marker for Osteoarthritis DOI Open Access
Kamila Baran, Ewa Brzeziańska‐Lasota, Jakub Kryczka

et al.

Journal of Clinical Medicine, Journal Year: 2025, Volume and Issue: 14(4), P. 1176 - 1176

Published: Feb. 11, 2025

Background/Objectives: Osteoarthritis (OA) is the most common degenerative and chronic joint disease a leading cause of pain disability in adults worldwide. The SRY-related HMG box (SOX) family transcription factors (TFs) play crucial role during pathogenesis OA; however, their exact mechanisms remain unexplored. aim our study was to conduct bioinformatics analysis interactions SOX-5, SOX-9, SOX-11 with other proteins, as well OA pathogenesis. Methods:SOX5, SOX9, SOX11 mRNA expression levels articular cartilage subchondral bone synovium from knee patients were assessed using qPCR method. group consisted thirty-one (n = 31). Total RNA isolated affected unaffected area joint. Results: Our results revealed regulatory network between SOX-11, various proteins involved collective interactions, which are regulation extracellular matrix (ECM) organization, response stimulus, gene expression, inflammatory response, condensation, ossification chondrocytes. Higher SOX5, noted OA-affected compared control tissue (p 0.00015, p 0.0024 > 0.05, respectively, Mann–Whitney U-test). All studied genes demonstrated elevated stage 4 than those 3 0.05; Lower found 0.0003, 0.05 0.0007, Decreased SOX9 3; SOX5 higher Conclusions: research show that SOX TFs development OA, contributing formation pathological changes not only cartilage, but also synovial membrane. may serve potential molecular diagnostic biomarkers for detecting could indicate progression this disease; observations require further investigation.

Language: Английский

Citations

1
Insights into dietary phytochemicals targeting Parkinson's disease key genes and pathways: A network pharmacology approach DOI

Devi Soorya Narayana Sasikumar,

Premkumar Thiruselvam,

Vino Sundararajan

et al.

Computers in Biology and Medicine, Journal Year: 2024, Volume and Issue: 172, P. 108195 - 108195

Published: Feb. 20, 2024

Language: Английский

Citations

7
Single‐cell sequencing unveils the impact of aging on the progenitor cell diversity in the telencephalon of the female killifish N. furzeri DOI Creative Commons
Rajagopal Ayana, Caroline Zandecki, Jolien Van houcke

et al.

Aging Cell, Journal Year: 2024, Volume and Issue: 23(10)

Published: July 1, 2024

Abstract The African turquoise killifish ( Nothobranchius furzeri ) combines a short lifespan with spontaneous age‐associated loss of neuro‐regenerative capacity, an intriguing trait atypical for teleost. impact aging on the cellular composition adult stem cell niches, leading to this dramatic decline in postnatal neuro‐ and gliogenesis, remains elusive. Single‐cell RNA sequencing telencephalon young female short‐lived GRZ‐AD strain unveiled progenitors glial non‐glial nature, different excitatory inhibitory neuron subtypes, as well non‐neural types. Sub‐clustering identified four radial glia (RG) types, two progenitor (NGP) intermediate (intercell) states. Two astroglia‐like, one ependymal, neuroepithelial‐like (NE) RG subtype were found at locations forebrain line their role, while proliferative, active NGPs spread throughout. Lineage inference pointed NE‐RG start intercessor populations glio‐ neurogenesis. Upon aging, single‐cell revealed major perturbations proportions astroglia intercell states, molecular signatures specific including altered MAPK, mTOR, Notch, Wnt pathways. This catalog regeneration‐competent telencephalon, combined evidence aging‐related transcriptomic changes, presents useful resource understand basis age‐dependent neuroplasticity. data is also available through online database killifishbrain_scseq ).

Language: Английский

Citations

4
SOXs: Master architects of development and versatile emulators of oncogenesis DOI

Saloni,

Manisha Sachan,

Rahul Johari

et al.

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: unknown, P. 189295 - 189295

Published: March 1, 2025

Language: Английский

Citations

0
SOX family transcription factors as therapeutic targets in wound healing: A comprehensive review DOI
Penghui Ye,

Rifang Gu,

Huan Zhu

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 253, P. 127243 - 127243

Published: Oct. 6, 2023

Language: Английский

Citations

4
Decoding vascular aging: implications for atherosclerosis progression and clinical intervention DOI Creative Commons
Silumbwe Ceaser Wankumbu,

Xiao‐Man Ji,

Ming Xu

et al.

Published: Aug. 2, 2024

Age-related pathologies, particularly cardiovascular disorders, pose a significant global health concern. The World Health Organization (WHO) predicts an increase in advanced mortality by 2030 unless critical interventions are implemented. Atherosclerosis remains the major cause of various diseases. Hence, this review focused on interaction between known mechanisms vascular aging, disease manifestation, and progression during atherosclerosis. In review, we highlighted five altered models: genomic instability, neurohormonal deregulation, epigenetics, protein regulation, gut microbiome. articles were selected from indexed scientific databases. It is important to note that equally interrelated with other aging pathways, such as inflammation senescence. conclusion, atherosclerosis multifaceted cholesterol-lowering therapy has been widely used. However, more than one specific action line required eradicate or slow down its manifestation. Equally, establishing balance stressors resulting injuries stress buffering treatment promising therapeutic targets reviewed include angiotensin (1–7)/MAS axis, microbiome, histone deacetylases, DNA repair systems, noncoding RNAs, β3/dopamine adrenoceptors, senescence checkpoints.

Language: Английский

Citations

1
The Effects of Health Promotion Program on Healthy Aging, Wellbeing and Health Promoting Behaviour among Community Dwelling Older Adults DOI Open Access
Eman Elsayed,

Eman Radwan,

Doaa El Sayed Fadila

et al.

NILES Journal forGeriatric and Gerontology/NILES Journal for Geriatric and Gerontology, Journal Year: 2024, Volume and Issue: 7(2), P. 417 - 442

Published: May 25, 2024

Background: One of the most essential needs all people is providing, enhancing health level, and adopting a lifestyle that promotes health. It significant practical way to keep older persons' wellbeing promote healthy aging. Aim: To investigate effects promotion program on aging, promoting behaviour among community dwelling adults. Methods: Four randomly selected centers out total 38 were used for study, which was carried in Mansoura District within Dakahlia governorate. Based inclusion exclusion criteria, purposive sample 94 adults from 110 persons enrolled above-mentioned setting selected, 75 included final analysis. Tools: tools used; Structured Interview Questionnaire, Healthy Aging Instrument (HAI), Modified Wellbeing Assessment Tool, Health Promoting Lifestyle Profile-II Questionnaire (HPLP-II). Results: All aging factors, domains, dimensions study group showed improvement after implementation (p <0.0001). This appears young old married female, with university education, who still working, had enough income, living family, good status. Also, highly statistically positive correlations (p<0.001) found between wellbeing, group. Conclusion: has effect adults' behaviour. Recommendations: decrease prevalence chronic illnesses multiple complications due lack lifestyle, it recommended implement programs.

Language: Английский

Citations

0
HES1 revitalizes the functionality of aged adipose-derived stem cells by inhibiting the transcription of STAT1 DOI Creative Commons
Chengcheng Li, Sen Ren, Chengqi Yan

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 5, 2024

The effectiveness of adipose-derived stem cells (ADSCs) in therapy diminishes with age. It has been reported that transcription factors (TFs) play a crucial role the aging and functionality cells. Nevertheless, there is limited understanding regarding involvement TFs mechanism ADSCs. RNA sequencing (RNA-seq) was utilized to discern differentially expressed genes ADSCs obtained from donors varying ages. exhibiting significant variations across age groups were identified subsequently validated. manipulated exhibit either enhanced expression or reduced levels HES1 STAT1 via lentivirus transfection small interfering (siRNA) techniques. impact these genetic alterations on ADSCs' proliferation, migration, cellular senescence assessed using EdU, transwell, senescence-activated β-galactosidase (SA-β-gal) staining assays. DNA sequences bound by investigated through CUT & Tag assay. Lastly, therapeutic efficacy aged overexpression evaluated skin injury model male Sprague-Dawley rats. 678 showed differential between young old (Y-ADSCs O-ADSCs), 47 being TFs. Notably, TF hairy enhancer split 1 (HES1) notably donors. Introducing resulted improved function suppression senescence, while reducing had opposite effect. Mechanistically, found interact promoter region another TF, signal transducer activator (STAT1), inhibit its transcription. Knocking down could fully reverse negative effects caused decreased ADSCs, leading reduction secretion pro-inflammatory cytokines such as TNF-α, IL-6, IL-8. Ultimately, restoring demonstrated potential promoting wound healing. acts an inhibitor progression modulation expression, suggesting promising avenue for rejuvenating senescent improving

Language: Английский

Citations

0
Single-Cell RNA-Seq Analysis Reveals Four Stages of Replicative Senescence in Human Umbilical Vein Endothelial Cells DOI Creative Commons

Minseo Ahn,

Junil Kim, Jae Ho Seo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 15, 2023

Abstract Cellular senescence is a phenomenon marked by an irreversible growth arrest with altered physiological properties. Many studies have focused on the characteristics of cells that already entered senescent state. However, to elucidate mechanisms cellular aging, it essential investigate gradual transition proliferative into cells. We assumed complex and multi-step process, each stage exhibits unique traits. To test this hypothesis, we utilized publicly available single-cell RNA-Seq (scRNA-Seq) data from human umbilical vein endothelial (HUVECs) undergoing replicative senescence. employed Seurat Monocle 3 capture proliferating states in HUVECs. Four clusters were identified, cluster displayed distinct expression patterns markers senescence-associated secretory phenotypes (SASPs). also SCENIC identify core transcription factors (TFs) during While majority TFs exhibited linear trend, HMGB1, FOSL1, SMC3, RAD21, SOX4, XBP1 showed fluctuating Furthermore, these slight differences ionizing radiation (IR) model Overall, our study unveils phase identifies trends may play pivotal roles process. These findings highlight intricate nature provide new insights process aging.

Language: Английский

Citations

0