Postoperative Organ Dysfunction Risk Stratification Using Extracellular Vesicle-Derived circRNAs in Pediatric Congenital Heart Surgery DOI Creative Commons
Fahd Alhamdan, Koichi Yuki

Cells, Journal Year: 2024, Volume and Issue: 13(17), P. 1417 - 1417

Published: Aug. 25, 2024

Breakthroughs in surgical and medical techniques have significantly improved outcomes for children with congenital heart disease (CHD), but research continues to address the ongoing challenge of organ dysfunction after surgery, particularly neonates infants. Our study explored circular RNAs (circRNAs) within plasma-derived extracellular vesicles (EVs) infants undergoing CHD surgery. Post-surgery EV circRNAs showed dramatic expression changes between (OD) control groups. Tissue injury-related pathways were consistent across pre- post-surgery OD. The top two significant predicted tissue sources these originated from respiratory system, aligning fact that all patients OD arm experienced dysfunction. Five circRNAs, namely circ-CELSR1, circ-PLXNA1, circ-OBSL1, circ-DAB2IP, circ-KANK1, correlated PELOD (Pediatric Logistic Organ Dysfunction) score demonstrated high performance (AUC = 0.95), supporting potential as prognostic markers. These findings pave way promising tools managing post-surgical potentially guiding therapeutic strategies CHD.

Language: Английский

Research trends and hotspots of circular RNA in cardiovascular disease: A bibliometric analysis DOI Creative Commons

Zehui Xu,

Chong Guan,

Ziji Cheng

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 9(3), P. 930 - 944

Published: April 17, 2024

From a global perspective, cardiovascular diseases (CVDs), the leading factor accounting for population mortality, and circRNAs, RNA molecules with stable closed-loop structures, have been proven to be closely related. The latent clinical value potential role of circRNAs in CVDs attracting increasing, active research interest, but bibliometric studies this field are still lacking. Thus, study, we conducted analysis by using software such as VOSviewer, CiteSpace, Microsoft Excel, R package determine current progress hotspots ultimately provide an overview development trends future frontiers field. In our based on search strategy, total 1206 publications published before July 31, 2023 were accessed from WOSCC database. According findings, there is notable increasing trend circRNA CVDs. China was found dominant country terms publication number, lack high-quality articles significant fault. A cluster co-cited references indicated that dilated cardiomyopathy, AMI, cardiac hypertrophy greatest objects concern. contrast, keywords high importance has ascribed MI, abdominal aortic aneurysm, cell proliferation, coronary artery diseases.

Language: Английский

Citations

4
Circular RNAs Variously Participate in Coronary Atherogenesis DOI Creative Commons

Liudmila V. Dergunova,

Margarita A. Vinogradina, Ivan B. Filippenkov

et al.

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 45(8), P. 6682 - 6700

Published: Aug. 13, 2023

Over the past decade, numerous studies have shown that circular RNAs (circRNAs) play a significant role in coronary artery atherogenesis and other cardiovascular diseases. They belong to class of non-coding arise as result non-canonical splicing premature RNA, which results formation closed single-stranded circRNA molecules lack 5′-end caps 3′-end poly(A) tails. circRNAs broad post-transcriptional regulatory activity. Acting sponge for miRNAs, compete with mRNAs binding acting competing endogenous RNAs. Numerous are involved circRNA–miRNA–mRNA axes associated pathogenesis cardiomyopathy, chronic heart failure, hypertension, atherosclerosis, disease. Recent сirc_0001445, circ_0000345, circ_0093887, сircSmoc1-2, circ_0003423 disease (CAD) an atheroprotective effect, while circ_0002984, circ_0029589, circ_0124644, circ_0091822, circ_0050486 possess proatherogenic effect. With their high resistance endonucleases, promising diagnostic biomarkers therapeutic targets. This review aims provide updated information on involvement atherogenesis-related CAD. We also discuss main modern approaches detecting studying interactions, well prospects using targets treatment

Language: Английский

Citations

10
Identification and validation of key genes associated with cell senescence in acute myocardial infarction DOI Creative Commons
Weidong Zhao,

Guofu Zhu,

Tianshu Chu

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 19, 2025

Cellular senescence can cause heart failure. However, studies on diagnostic markers related to cellular in acute myocardial infarction (AMI) have not been reported. Therefore, this study explores the mechanism of key genes through a bioinformatics approach. AMI datasets were obtained from gene expression omnibus (GEO) database, and 3,058 (CSRGs) extracted Molecular Signatures Database (MSigDB). First, differentially expressed (DEGs) screened by differential analysis,and then Weighted co-expression network analysis (WGCNA) was performed module. CSRGs, DEGs module intersected acquire genes, candidate also out constructing protein interaction (PPI) network.Afterwards, subjected machine learning approach identify enrichment analyses individual Finally, immuno-infiltrative analysis, competing-endogenous RNA (ceRNA) drug-gene networks construction conducted. Besides, validated quantitative real-time polymerase chain reaction (qRT-PCR). Screening for two (ATP6V0B DYNLL1) 914 DEGs, they involved functional pathways such as 'mast cell activation', 'cytosolic ribosome'. Thereafter, we found infiltration neutrophils, CD4 T cells memory resting gamma delta notably different between control samples. Pearson correlation suggested that neutrophils had highest positive with ATP6V0B (Cor = 0.7), while negative DYNLL1 -0.6). The ceRNA one mRNA (DYNLL1), miRNA (hsa-miR-330-5p) four circRNAs. Eventually, drug-ATP6V0B 74 nodes 73 edges, drug-DYNLL1 119 118 edges. qRT-PCR trend consistent result bioinformatic analysis. Notably, significantly down-regulated case group. Identified AMI, which is great significance diagnosis molecular targeted therapy AMI.

Language: Английский

Citations

0
Physical exercise and epigenetic modifications in skeletal muscle, brain, and heart DOI Creative Commons

Xi Zheng,

Xueli Liu, Yuqian Guo

et al.

Epigenetics & Chromatin, Journal Year: 2025, Volume and Issue: 18(1)

Published: March 21, 2025

The origins of many diseases can be traced to the dynamic interplay genetic predispositions and environmental exposures post-birth. Epigenetic modifications have recently gained prominence as a significant mediator between information factors, influencing occurrence progression disease. There is burgeoning body evidence supports that physical exercise, acting an external stimulus, exerts discernible impact on major epigenetic modifications, including histone DNA methylation, RNA non-coding RNA. This effect assumes pivotal role in pathogenesis various human diseases. Exploring molecular mechanisms through which exercise enhances health holds promise deepening our understanding how it improves physiological functions, mitigates disease risks, establishes theoretical foundation for employing non-pharmacological intervention prevention treatment.

Language: Английский

Citations

0
RNA-mediated epigenetic regulation in exercised heart: Mechanisms and opportunities for intervention DOI
Lijun Wang,

Wensi Wan,

Shuang Zhang

et al.

Molecular Aspects of Medicine, Journal Year: 2024, Volume and Issue: 97, P. 101274 - 101274

Published: April 22, 2024

Language: Английский

Citations

2
Circ_0008571 modulates the phenotype of vascular smooth muscle cells by targeting miR-145-5p in intracranial aneurysms DOI
Zhiwen Lu,

Shijie Zhu,

Yina Wu

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(6), P. 167278 - 167278

Published: June 2, 2024

Language: Английский

Citations

2
Non-Coding RNA-Mediated Gene Regulation in Cardiovascular Disorders: Current Insights and Future Directions DOI
Muhammad Abubakar,

Mohsin Hajjaj,

Zil e Zehra Naqvi

et al.

Journal of Cardiovascular Translational Research, Journal Year: 2023, Volume and Issue: 17(4), P. 739 - 767

Published: Dec. 13, 2023

Language: Английский

Citations

5
Potential new therapeutic targets: Association of microRNA with atherosclerotic plaque stability DOI Creative Commons
Pan Huang

International Journal of Immunopathology and Pharmacology, Journal Year: 2023, Volume and Issue: 37

Published: July 5, 2023

Cardiovascular disease represents a major threat to human health with vascular atherosclerosis contributing significantly its cause, particularly due the ease of rupture atherosclerotic plaques. The stability plaques is influenced by variety factors, including intraplaque neovascularization, inflammatory response, smooth muscle cells and macrophages, core lipid volumes. Thus, investigation factors affecting great significance for development new drugs treatment diseases. MicroRNAs are small single-stranded non-coding RNAs between 17 22 nucleotides in length. They translated together untranslated region (UTR) target gene mRNA, where completeness base-pairing can influence translation or degradation genes. thus regulate expression at post-transcriptional level have been demonstrated be extensively involved regulation various plaque stability. In this paper, we review microRNA development, stability, relationship microRNAs aim describing mechanisms which affect protein relation progression (including rupture) suggest therapeutic targets disease.

Language: Английский

Citations

4
Exploring circular RNAs as biomarkers for Parkinson’s disease and their expression changes after aerobic exercise rehabilitation DOI
Flávia Maria Campos de Abreu,

Deborah Almeida de Oliveira,

Sabrina Simplício de Araujo Romero Ferrari

et al.

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(4)

Published: July 29, 2024

Language: Английский

Citations

1
Down-regulation of the Smad signaling by circZBTB46 via the Smad2-PDLIM5 axis to inhibit type I collagen expression DOI Open Access
Jing Yu,

Wen-Zhao Yan,

Xinhua Zhang

et al.

Journal of Geriatric Cardiology, Journal Year: 2023, Volume and Issue: 20(6), P. 431 - 447

Published: June 1, 2023

Abnormal type I collagen (COL1) expression is associated with the development of many cardiovascular diseases. The TGF-beta/Smad signaling pathway and circRNAs have been shown to regulate COL1 gene expression, but underlying molecular mechanisms are still not fully understood.Gain- loss-of-function experiments were prformed study effect circZBTB46 on alpha 2 chain (COL1A2). Co-immunoprecipitation assay was performed observe interaction between two proteins. RNA immunoprecipitation biotin pull-down PDLIM5.In this study, we investigated role in regulating COL1A2 human vascular smooth muscle cells (VSMCs). We found that expressed VSMCs TGF-beta inhibits formation by downregulating KLF4 through activation Smad pathway. CircZBTB46 induced TGF-beta. Mechanistically, mediates Smad2 PDLIM5, resulting inhibition subsequent downregulation expression. Furthermore, decreased, while increased abdominal aortic aneurysm tissues, indicating circZBTB46-mediated regulation synthesis plays a crucial homeostasis development.CircZBTB46 identified as novel inhibitor VSMCs, highlighting importance PDLIM5

Language: Английский

Citations

3